Dying without Dignity: Homeless Deaths in Los Angeles County: 2000 - 2007

This report is an investigation into 2,815 homeless deaths in Los Angeles County between January, 2000 and May, 2007, based on statistics provided by the Los Angeles County Coroner's office. When a homeless person dies they do not often get the same sense of dying with dignity as a housed person. December 21st has been commemorated as the National Homeless Persons' Memorial Day by the National Coalition for the Homeless in partnership with the National Health Care for the Homeless Council for communities around the nation to commemorate the lives of homeless people that passed away.Local advocates and service providers celebrate the lives of thousands of homeless people in hundreds of cities around the nation with candlelight vigils, a reading of names, and other acts to remember the lives of those lost while living on the streets of our nation.This report is an investigation into homeless deaths in Los Angeles County between January, 2000 and May, 2007, based on statistics from the Los Angeles County Coroner's office. It is our hope that the homeless people who make up the statistics in this report did not die in vain and that policy makers move to implement the recommendations of this report in an effort to provide the dignity they did not find while living on the streets of our community. Equally important, to implement these strategies to help prevent the untimely deaths of homeless people in the future

AGRICULTURAL INDUSTRIALIZATION AND SUSTAINABLE DEVELOPMENT: A GLOBAL PERSPECTIVE

Agricultural industrialization and sustainable development issues are important contemporary areas of debate. This paper argues that the two processes are a consequence of a set of forces operating in our global system. It outlines a number of conceptual interactions between the two phenomena and examines economic development and sustainable development policy implications that appear to be logical extensions of the arguments presented.Agricultural industrialization, Sustainable agricultural development, Development assistance, Human capital, Market and policy failures, Public policy, Environmental Economics and Policy, Research and Development/Tech Change/Emerging Technologies,

Quick guide to monitoring economic impacts of ecosystem restoration and stewardship

16 pagesThis guide will help you develop an economic monitoring program by setting goals, selecting monitoring measures, collecting data, and reporting and using monitoring results. Specifically, it describes how to obtain and utilize detailed information about job numbers, job quality, wages, contracting and subcontracting opportunities, and other related economic impacts of restoration.This quick guide was made possible with funding from the Meyer Fund for a Sustainable Environment

Identification of Telomerase RNAs from Filamentous Fungi Reveals Conservation with Vertebrates and Yeasts

Telomeres are the nucleoprotein complexes at eukaryotic chromosomal ends. Telomeric DNA is synthesized by the ribonucleoprotein telomerase, which comprises a telomerase reverse transcriptase (TERT) and a telomerase RNA (TER). TER contains a template for telomeric DNA synthesis. Filamentous fungi possess extremely short and tightly regulated telomeres. Although TERT is well conserved between most organisms, TER is highly divergent and thus difficult to identify. In order to identify the TER sequence, we used the unusually long telomeric repeat sequence of Aspergillus oryzae together with reverse-transcription-PCR and identified a transcribed sequence that contains the potential template within a region predicted to be single stranded. We report the discovery of TERs from twelve other related filamentous fungi using comparative genomic analysis. These TERs exhibited strong conservation with the vertebrate template sequence, and two of these potentially use the identical template as humans. We demonstrate the existence of important processing elements required for the maturation of yeast TERs such as an Sm site, a 59 splice site and a branch point, within the newly identified TER sequences. RNA folding programs applied to the TER sequences show the presence of secondary structures necessary for telomerase activity, such as a yeast-like template boundary, pseudoknot, and a vertebrate-like three-way junction. These telomerase RNAs identified from filamentous fungi display conserved structural elements from both yeast and vertebrate TERs. These findings not only provide insights into the structure and evolution of a complex RNA but also provide molecular tools to further study telomere dynamics in filamentous fungi

Drum vortons in high density QCD

Recently it was shown that high density QCD supports of number of topological defects. In particular, there are U(1)_Y strings that arise due to K^0 condensation that occurs when the strange quark mass is relatively large. The unique feature of these strings is that they possess a nonzero K^+ condensate that is trapped on the core. In the following we will show that these strings (with nontrivial core structure) can form closed loops with conserved charge and currents trapped on the string worldsheet. The presence of conserved charges allows these topological defects, called vortons, to carry angular momentum, which makes them classically stable objects. We also give arguments demonstrating that vortons carry angular momentum very efficiently (in terms of energy per unit angular momentum) such that they might be the important degrees of freedom in the cores of neutron stars.Comment: 11 pages, accepted for publication in Physical Review

Fact sheet: The impacts of the woody biomass utilization program in Eastern Arizona

Utilizing woody biomass - small-diameter material and low-valued trees removed from forest restoration activities - on public lands may help reduce agency costs, enhance community wildfire protection, and create employment and economic activity. Yet small businesses adjacent to public land often lack the capacity to harvest and utilize biomass. Businesses face challenges such as limited access to capital and markets, technical assistance, and inconsistent material supply. From 2005-2010, the USDA Forest Service's Woody Biomass Utilization Grant (Woody BUG) program provided resources to address these barriers. We evaluated and compared the impacts of this program in eastern Oregon and in eastern Arizona. Here we summarize the findings from eastern Arizona

UV and infrared absorption spectra, atmospheric lifetimes, and ozone depletion and global warming potentials for CCl2FCCl2F (CFC-112), CCl3CClF2 (CFC-112a), CCl3CF3 (CFC-113a), and CCl2FCF3 (CFC-114a)

Abstract. The potential impact of the recently observed CCl2FCCl2F (CFC-112), CCl3CClF2 (CFC-112a), CCl3CF3 (CFC-113a), and CCl2FCF3 (CFC-114a) (chlorofluorocarbons, CFCs), on stratospheric ozone and climate are presently not well characterized. In this study, the UV absorption spectra of these CFCs were measured between 192.5–235 nm over the temperature range 207–323 K. Precise parameterizations of the UV absorption spectra are presented. A 2-D atmospheric model was used to evaluate the CFC atmospheric loss processes, lifetimes, ozone depletion potentials (ODPs), and the associated uncertainty ranges in these metrics. The CFCs are primarily removed in the stratosphere by short wavelength UV photolysis with calculated global annually averaged steady-state lifetimes (years) of 63.6 (61.9–64.7), 51.5 (50.0–52.6), 55.4 (54.3–56.3), and 105.3 (102.9–107.4) for CFC-112, CFC-112a, CFC-113a, and CFC-114a, respectively. The range of lifetimes given in parentheses where obtained by including the 2σ uncertainty in the UV absorption spectra and O(1D) rate coefficients in the model calculations. The 2-D model was also used to calculate the CFC ozone depletion potentials (ODPs) with values of 0.98, 0.86, 0.73, and 0.72 obtained for CFC-112, CFC-112a, CFC-113a, and CFC-114a, respectively. Using the infrared absorption spectra and lifetimes determined in this work, the CFCs global warming potentials (GWPs) were estimated to be 4260 (CFC-112), 3330 (CFC-112a), 3650 (CFC-113a), and 6510 (CFC-114a) for the 100-year time-horizon. </jats:p

A human embryonic kidney 293T cell line mutated at the Golgi -mannosidase II locus

Disruption of Golgi -mannosidase II activity can result in type II congenital dyserythropoietic anemia and can induce lupus-like autoimmunity in mice. Here, we isolate a mutant human embryonic kidney (HEK) 293T cell line, called Lec36, that displays sensitivity to ricin that lies between the parental HEK 293T cells, whose secreted and membrane-expressed proteins are dominated by complex-type glycosylation, and 293S Lec1 cells, which only produce oligomannose-type N-linked glycans. The stem cell marker, 19A, was transiently expressed in the HEK 293T Lec36 cells, and in parental HEK 293T cells with and without the potent Golgi -mannosidase II inhibitor, swainsonine. Negative-ion nano-electrospray ionization mass spectra of the 19A N-linked glycans from HEK 293T Lec36 and swainsonine-treated HEK 293T cells were qualitatively indistinguishable and, as shown by collision-induced dissociation spectra, dominated by hybrid-type glycosylation. Nucleotide sequencing revealed mutations in each allele of MAN2A1, the gene encoding Golgi -mannosidase II: a point mutation in one allele mapping to the active site and an in-frame deletion of twelve-nucleotides in the other. Expression of wild-type but not the mutant MAN2A1 alleles in Lec36 cells restored processing of the 19A reporter glycoprotein to complex-type glycosylation. The Lec36 cell line will be useful for expressing therapeutic glycoproteins with hybrid-type glycans and provides a sensitive host for detecting mutations in human MAN2A1 causing type II congenital dyserythropoietic anemia

Ultraparamagnetic cells formed through intracellular oxidation and chelation of paramagnetic iron

Making cells magnetic is a long‐standing goal of chemical biology, aiming to enable the separation of cells from complex biological samples and their visualization in vivo using magnetic resonance imaging (MRI). Previous efforts towards this goal, focused on engineering cells to biomineralize superparamagnetic or ferromagnetic iron oxides, have been largely unsuccessful due to the stringent required chemical conditions. Here, we introduce an alternative approach to making cells magnetic, focused on biochemically maximizing cellular paramagnetism. We show that a novel genetic construct combining the functions of ferroxidation and iron chelation enables engineered bacterial cells to accumulate iron in “ultraparamagnetic” macromolecular complexes, allowing these cells to be trapped with magnetic fields and imaged with MRI in vitro and in vivo. We characterize the properties of these cells and complexes using magnetometry, nuclear magnetic resonance, biochemical assays, and computational modeling to elucidate the unique mechanisms and capabilities of this paramagnetic concept