Genetic Requirements for Intra-Chromosomal Deletions

Chromosomal deletions are one of the most dangerous types of DNA damage and often arise as a result of inappropriately repaired DNA double strand breaks (DSB). These breaks are usually formed either in an induced manner from exogenous damage such as radiation, or more commonly caused from spontaneous replication errors. If there is a single strand break during replication and it is not repaired properly, as the replication fork progresses it can lead to the formation of a DSB. When there is a DSB present, there is the opportunity for a chromosomal deletion to occur. If the break is in between non-tandem direct repeats, the DNA repair machinery will degrade what is between the direct repeats through a process called Single Strand Annealing (SSA). This massive loss of DNA is what is known as a chromosomal deletion. Using an assay that in Schizosaccharomyces pombe, we can detect DSBs and determine DNA repair pathways through a selection screen of yeast cells with inactivated DNA repair genes. We generated an in vivo assay that reports exclusively SSA. We validated the assay by showing its dependence on rad52+ and independence rad51+. However, we show that earlier events epistatic to rad52+ and rad51+ have differential requirements for deletions vs. other forms of repair. Here, we delineate a more detailed epistatic pathway for intrachromosomal deletions.No embargoAcademic Major: Biolog

The Ethical Basis for Veganism

This chapter examines the ethical case that can be mounted for veganism. Because there has been comparatively little discussion in ethics focused directly on veganism, the central aim of this chapter is threefold: to orient readers to (some of) the most important philosophical literature relevant to the topic, to provide a clear explanation of the current state of the ethical case for veganism, and to focus attention on the most important outstanding or underexplored questions in this domain. The chapter examines the range of positions that deserve to be called ethical veganism, and some of the types of reasons that philosophers can potentially appeal to in arguing for veganism. It then spells out the core of the most promising case for veganism, which argues directly for the wrongness of making animals suffer and die. The chapter then considers three ways of arguing from this conclusion to an ethical defense of the vegan lifestyle, which appeal respectively to the ethical significance of the effects of individual use of animal products, of group efficacy, and of complicity with wrongdoing. The chapter concludes by examining several neglected complications facing the ethical case for veganism

A Key and Annotated List of the Scutelleroidea of Michigan (Hemiptera)

Excerpt: Although Hussey (1922) compiled a list of the Hemiptera of Berrien County, and Stoner (1922) contributed a list of the Scutelleroidea of the Douglas Lake region, no publications have dealt with Michigan Scutelleroidea on a state-wide basis. However, collections in the Entomology Museum of Michigan State University (MSU), East Lansing, and in the Museum of Zoology of the University of Michigan (UMMZ), Ann Arbor, indicate that collecting has been extensive throughout the state (Fig. 1). The key and annotated list are based on material I identified in these two collection

A Rule Set for the Future

This volume, Digital Young, Innovation, and the Unexpected, identifies core issues concerning how young people's use of digital media may lead to various innovations and unexpected outcomes. The essays collected here examine how youth can function as drivers for technological change while simultaneously recognizing that technologies are embedded in larger social systems, including the family, schools, commercial culture, and peer groups. A broad range of topics are taken up, including issues of access and equity; of media panics and cultural anxieties; of citizenship, consumerism, and labor; of policy, privacy, and IP; of new modes of media literacy and learning; and of shifting notions of the public/private divide. The introduction also details six maxims to guide future research and inquiry in the field of digital media and learning. These maxims are "Remember History," "Consider Context," "Make the Future (Hands-on)," "Broaden Participation," "Foster Literacies," and "Learn to Toggle." They form a kind of flexible rule set for investigations into the innovative uses and unexpected outcomes now emerging or soon anticipated from young people's engagements with digital media

A List of the Scutelleroidea (Hemiptera) of Southern Illinois

A list of the Scutelleroidea of southern Illinois is presented with information on the state distribution of each species

Italian via email: From an online project of learning and teaching towards the development of a multi‐cultural discourse community

This paper seeks to illustrate how the use of Internet resources (specifically email and the Web) can affect and enhance language learning and cultural understanding, modify the learning environment, reduce the barriers which time, space and societal differences may create, be a source of motivation, and redefine the role of teachers and learners. Although it is based on an on‐going project, it already provides practical evidence of some advantages email and Internet resources can bring to the language learner and to the teacher. A detailed evaluation of the language outcomes is under way, but incomplete at the time of writing. This paper is nevertheless more concerned with other variables of language learning and teaching which the author considers fundamental to reach a successful degree of language use

Life History of \u3ci\u3eNeoplea Striola\u3c/i\u3e (Hemiptera: Pleidae)

The life history of Neoplea striola was studied in southern Illinois during 1983-1985. This apparently univoltine species overwintered as adults and became active in early March. First instars appeared in mid-May followed by marked overlapping of the subsequent instars. No active adults were found after November

Noteworthy Range Extensions of Three Emesine Species (Heteroptera: Reduviidae)

The first records of Empicoris culiciformis and E. winnemana from Michigan and of Pseudometapterus umbrosus from Illinois are reported. All represent considerable extensions of their recorded ranges

Life History of \u3ci\u3eMesovelia Mulsanti\u3c/i\u3e (Hemiptera: Mesoveliidae) in Southern Illinois

The life history of Mesovelia mulsanti was studied during 1983-1986. This species was apparently trivoltine with a partial 4th generation at the end of the year. It overwintered as eggs. First ins tars appeared in mid-April followed by marked overlapping of the subsequent instars and generations. The first adults appeared in early May. No active individuals were found after November