Diagnostic value of serum thiobarbituric acid reactive substances levels in pediatric acute appendicitis

Purpose: This study aims to show the diagnostic value of Serum Thiobarbituric Acid Reactive Substances in pediatric appendicitis. Method: Eighty-five pediatric patients hospitalized in the pediatric surgery ward with acute appendicitis and a control group of 50 pediatric patients with unspecific abdominal pain were included in this prospective case-control study. Forty-five patients whose pathology specimens confirmed acute appendicitis made up the final appendicitis group. Results: Patients with appendicitis had higher Serum Thiobarbituric Acid Reactive Substances (p<0.001) levels than the control group. In receiver operating characteristic analysis, areas under the curve were 0.654 for Serum Thiobarbituric Acid Reactive Substances. Conclusion: Serum Thiobarbituric Acid Reactive Substances test of patients with appendicitis provides limited accuracy in the diagnosis of appendicitis

Protective roles of Cimin grape tissues on oxidative stress markers in the cellular system model

Aim: To analyze the phenolic composition of the Cimin grape, which is 1 of 2 grape varieties with a protected geographical indication in Turkey and is used locally to treat the symptoms of some disorders such as impotence and cardiovascular diseases, and to investigate its antioxidant potency against oxidant mediators in the models of intra- and extracellular surroundings. Materials and methods: Cimin grape samples were classified into 5 groups according to the grape’s tissues and the extraction solvents used. Free radical scavenging (DPPH) and antilipid peroxidation product [thiobarbituric acid reactive substance (TBARS) and conjugated diene (CD)] levels of the grape tissues were extrapolated from the measurement of total phenolic and individual monomeric flavanol contents in each extract. Results: The seed methanolic extract had the highest total amount of flavanols, with the amount of catechin at 4.034 mM. The DPPH activities of the seed extracts were 2- to 10-fold higher than those of the other samples. The seed extract-treated erythrocyte and unfractionated human plasma also showed lower TBARS and CD values. In addition, regeneration of glutathione was more obvious in grape seed extracts than in the rest of the tissues. Conclusion: The underlying mechanism of these changes can be related mainly to increased antioxidant status. Cimin grape consumption may have beneficial effects on health maintenance

The effectiveness of electro-acupuncture in ovarian ischemia reperfusion injury

Electro-acupuncture is widely used in gynecology. Adnexal torsion is an important threat to ovarian reserves in women of reproductive age. This is the first study to investigate whether electro-acupuncture is beneficial in protecting ovarian reserves in case of adnexal torsion resulting in reperfusion injury. Thirty-two female Wistar Albino rats were randomized into four groups: the first group-sham operation, the second group-torsion/detorsion model, the third group-pre-acupunture + torsion/detorsion + post-acupuncture, and the fourth group-torsion/detorsion + post-acupuncture. The acupoints used were CV4 and bilateral SP6, Ex-CA1, Kid3, and ST36. In the third group, the acupoints were needled for two weeks before torsion, continuing for a further two weeks after torsion. In the fourth group, needling began after torsion and was maintained for two weeks. Both histological and biochemical parameters indicating ovarian reserves showed that electro-acupuncture applied to the above points exhibited an ameliorating effect on ovaries injured during ischemia/reperfusion. Electro-acupuncture may be capable of protecting against and preventing ischemia/reperfusion injury in case of ovarian torsion

Association of inflammatory and ischemic markers with posterior segment parameters in pseudoexfoliation syndrome and glaucoma

Objective: This study aimed to investigate the structural, vascular, and biochemical alterations in patients with pseudoexfoliation syndrome (PES) and pseudoexfoliative glaucoma (PXG) and to evaluate the associations between serum biomarkers, the retinal nerve fiber layer (RNFL), choroidal thickness (CT), and vessel density (VD) in these groups. Methods: All subjects underwent spectral-domain optical coherence tomography (SD-OCT) and OCT angiography (OCTA) to assess RNFL thickness, CT, and VD. Serum levels of inflammatory and oxidative stress biomarkers—including malondialdehyde (MDA), glutathione (GSH), interleukin-6 (IL-6), nitric oxide (NO), inducible NO synthase (iNOS), galectin-3, and SCUBE-1—were analyzed, and regression and ROC curve analyses were performed to evaluate predictive value and diagnostic performance. Results: A total of 80 patients were included and are listed as follows: 25 controls, 30 with PES, and 25 with PXG. There were no significant differences among groups in terms of age or gender. RNFL thickness, CT, and VD were significantly reduced in the PXG group compared to the PES and control groups (p < 0.001). PXG patients showed the most pronounced reductions in both peripapillary and macular CT, as well as superficial and deep VD. Serum iNOS, SCUBE-1, galectin-3, and MDA levels were significantly elevated in PXG, while GSH levels were lower (p < 0.001); NO levels showed no significant differences. In the PES and PXG groups, several ocular parameters correlated significantly with serum biomarkers, particularly iNOS, MDA, and GSH. Regression analysis in PXG patients identified iNOS and MDA as significant predictors of RNFL thickness and VD. ROC analysis demonstrated that MDA and GSH exhibited the highest diagnostic accuracy among the tested biomarkers for distinguishing PXG patients from controls. Conclusions: PXG is associated with significant structural, vascular, and biochemical alterations, including reduced RNFL thickness, choroidal thinning, and decreased VD. Altered serum levels of MDA and GSH were significantly associated with these ocular changes and demonstrated the highest diagnostic accuracy among the biomarkers evaluated. These findings support their potential utility as non-invasive biomarkers for distinguishing PXG from PES and healthy controls and for monitoring disease progression

The effect of white tea on serum TNF-α/NF-κB and immunohistochemical parameters in cisplatin-related renal dysfunction in female rats

The study was funded by a grant from the scientific research foundation of Recep Tayyip Erdogan University .Objective: Nephrotoxicity is the most important side effect of the antineoplastic drug cisplatin, thereby restricting its use. The aim of this study was to investigate the protective effects of white tea infusions (WT) against renal damage induced by cisplatin (CP) in rats by biochemical and histopathological means. Materials and methods: This study used 24 female Sprague Dawley rats at 12–14 weeks of age and weighing 250–300 g. Rats were divided into three groups: Control, CP and CP + WT groups. CP was injected 7 mg/kg i.p as a single dose/rat in the CP group. White tea was given at a dose of 0.5% (w/v) for 4 weeks. At the end of the experiment, blood urea nitrogen (BUN), creatinine, uric acid, tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6) and nuclear factor kappa B (NF-κB) along with caspase-3 in the kidney were evaluated in study. Results: BUN, creatinine, TNF-α NF-κB and IL-6 levels of the CP group showed a statisically significant increase in comparison to the control group. TNF-α NF-κB and IL-6 levels showed a statistically significant decrease in the CP + WT group with respect to the CP group. Caspase-3 levels in tubular epithelial cells decreased in CP + WT group compared with CP group (p = 0.02). Conclusion: White tea infusions reduced significantly the nephrotoxicity of CP. The anti-nephrotoxic feature of the infusion may be attributed primarily to its anti-inflammatory and anti-apoptotic characteristics

Comparison of inflammatory biomarkers for detection of coronary stenosis in patients with stable coronary artery disease

Erdogan, Turan/0000-0003-2986-5457; Cetin, Mustafa/0000-0001-6342-436X; yilmaz, adnan/0000-0003-4842-1173WOS: 000319223400015PubMed: 23329531BACKGROUND: the objective of the current study was to evaluate the role of various inflammatory biomarkers in detection of coronary stenosis in patients with stable coronary artery disease (CAD) and healthy people. METHODS: A total of 111 patients with stable coronary artery disease, and 66 healthy subjects were enrolled in the study. Serum levels of lipoprotein-associated-phospholipase A2 (Lp-PLA(2)), high-sensitivity C-reactive protein (hs-CRP), and myeloperoxidase (MPO) were measured to compare patient and control groups. RESULTS: Baseline characteristics were similar between healthy and patient groups, with the exception of age. ANCOVA and log-transformed data of inflammatory biomarkers revealed that, Lp-PLA(2) (p < 0,001) and hs-CRP (p < 0,05) levels in all patient groups were significantly higher than in the control group. Conversely, there was no significant difference in MPO levels among groups. CONCLUSIONS: in stable CAD patients, serum Lp-PLA(2) levels are more compatible than hsCRP and MPO levels in the detection of coronary stenosis.PfizerPfizer; ServierServier; Astra ZenecaAstraZenecaThis study was supported by the drug companies Pfizer, Servier and Astra Zeneca. the Authors thank all of the companies that helped during this investigation

Investigation of relationship between a non-coding rs599839 variant associated with sortilin (Sort1) gene region and lipoprotein subfractions in patients with coronary artery disease

Bu çalışmada Sort1 gen ekspresyonunun lipoprotein altsınıfları ve KAH ile ilişkisini incelendi. Ayrıca Sort1 ile aynı lokusta yer alan ve genin ekspresyonunu etkileyen rs599839 polimorfizmi çalışıldı. Çalışma grupları; anjiyografi sonucu çeşitli düzeylerde damar tıkanıklığı tespit edilen 162 hasta ve 49 sağlıklı bireylerden oluşturulmuştur. Çalışma gruplarında lipit profilleri (TAG, TK, LDL, HDL), serum apolipoprotein (ApoA, ApoB ve ApoE) seviyeleri ve CRP değeri otoanalizörde çalışıldı. Lipoproten altsınıfları Qantimetrix lipoprint sistem kullanılarak analiz edildi. Sort1 gen ekspresyonu düzeyi ise Human Sortilin TaqMan Gene Expression Assays kullanılarak qRT-PCR metoduyla ile belirlendi. Genomik DNA tam kandan izole edildi ve rs599839 polimorfiziminin belirlenmesi için genotipleme Polimeraz Zincir Reaksiyonu-Restiriksiyon Fragment Uzunluk Polimorfizmi (PCR-RFLP) metadu ile çalışıldı. Kontrol grubuna göre KAH grubunda Sort1 gen ekspresyonu anlamlı derecede artmıştır (p0,05). Çalışma grubunda lipoprotein altsınıfları karşılaştırıldığında; kontrol grubuna göre KAH'da aterojenik altfraksiyonlar (SdLDL-K ve SHDL) istatistiksel olarak daha yüksek bulunmuştur (p 0,05) were identified in Sort1 gene expression between variant allele groups of rs599839. When the lipoprotein subclasses were evaluated in the study groups; atherogenic subfractions (SdLDL-K and SHDL) were found to be statistically higher in CAD than control group (p <0,01). Patients with CAD had significantly higher levels of plasma atherogenic index (AIP), TG / HDL-C, Apo B / Apo AI, SdLDL / LbLDL and low LDL-C / Apo B levels compared to the controls (p <0,05). In conclusion, it has been found that Sort1 gene expression particularly has a linear relationship with both the atherogenic LDL phenotype and CAD

To determine LDL phenotypes using lipids, lipoproteins, apoproteins, and sdLDL through association rule mining

Objective: The atherogenic lipoprotein phenotype is closely associated with the risk assessment of Coronary Artery Disease (CAD) and the monitoring of treatment processes. Particularly, high levels of small dense low-density lipoprotein (sdLDL) and low levels of large buoy-ant low-density lipoprotein (lbLDL) are critical in determining Pattern B. This study aims to determine the lipid phenotype using the Association Rule Mining (ARM) method, based on concentrations of lipids, lipoproteins, apoproteins, and sdLDL.Materials and Methods: This retrospective case-control study utilized analytical research methods. Numerical variables were expressed as mean, standard deviation, median, and min-max values. Statistically significant differences were observed between the low-density lipoprotein (LDL) size categories in terms of triglycerides (TG), LDL, high-density lipoprotein (HDL), apolipoprotein B (ApoB), apolipoprotein E (ApoE), sdLDL, and lbLDL distributions. ARM was employed to detect the lipoprotein phenotype.Results: Statistically significant differences were found between the LDL size categories in distributions of TG, LDL, HDL, ApoB, ApoE, sdLDL, and lbLDL (p(TG)<0.001, p(LDL)=0.03, p(HDL)<0.001, p(ApoB)=0.016, p(ApoE)=0.004, p(sdLDL)<0.001, and p(lbLDL)<0.001). The ARM method revealed that the probability of phenotype B is 100% for sdLDL values in the range of 15.5-109 and lbLDL values in the range of 0-31.5.Conclusion: This study introduces a contemporary approach for detecting lipoprotein phenotypes using ARM, further substantiating the strong correlation between atherogenic phenotypes and sdLDL

Effect of white tea consumption on serum leptin, tnf-alpha and body weight in menopausal model rats

This work was supported by RTEU-BAP, TSA-2017-816.[No Asbtact Available]Turkish Neuroendocrinol So