Raufutter als Alleinfutter für Kaninchen – Auswirkungen auf das Fettsäurenmuster des Fleisches

Four groups of eight New Zealand hybrid rabbits were fed ryegrass meal only, alfalfa meal only, ryegrass and oats 1:1, or alfalfa and oats 1:1. Diets were offered ad libitum in pelleted form from 5-30 weeks of age, when they were slaughtered. Intramuscular fatty acid profiles were determined in the Musculus quadriceps of the left hindleg. Feed intake was not statistically different between the four groups, nor was carcass weight. The main effect of the forage-only diets on the fatty acid profiles was a decrease of monounsaturated and an increase of polyunsaturated fatty acid (PUFA) proportions. Within PUFA, the n-3 fatty acids more than doubled with forage-only compared to forage-oats diets, while the n-6 fatty acids slightly decreased. In general, the proportion of n-3 fatty acids in intramuscular fat of forage-only fed rabbits was extraordinarily high compared to any other meat of agricultural origin. The results demonstrate a specific advantage of roughage-based diets in the nutrition of productive herbivores, which is also known for ruminants

Multi-Dimensional, Compressible Viscous Flow on a Moving Voronoi Mesh

Numerous formulations of finite volume schemes for the Euler and Navier-Stokes equations exist, but in the majority of cases they have been developed for structured and stationary meshes. In many applications, more flexible mesh geometries that can dynamically adjust to the problem at hand and move with the flow in a (quasi) Lagrangian fashion would, however, be highly desirable, as this can allow a significant reduction of advection errors and an accurate realization of curved and moving boundary conditions. Here we describe a novel formulation of viscous continuum hydrodynamics that solves the equations of motion on a Voronoi mesh created by a set of mesh-generating points. The points can move in an arbitrary manner, but the most natural motion is that given by the fluid velocity itself, such that the mesh dynamically adjusts to the flow. Owing to the mathematical properties of the Voronoi tessellation, pathological mesh-twisting effects are avoided. Our implementation considers the full Navier-Stokes equations and has been realized in the AREPO code both in 2D and 3D. We propose a new approach to compute accurate viscous fluxes for a dynamic Voronoi mesh, and use this to formulate a finite volume solver of the Navier-Stokes equations. Through a number of test problems, including circular Couette flow and flow past a cylindrical obstacle, we show that our new scheme combines good accuracy with geometric flexibility, and hence promises to be competitive with other highly refined Eulerian methods. This will in particular allow astrophysical applications of the AREPO code where physical viscosity is important, such as in the hot plasma in galaxy clusters, or for viscous accretion disk models.Comment: 26 pages, 21 figures. Submitted to MNRA

Stable expression and functional characterization of a Na+-taurocholate cotransporting green fluorescent protein in human hepatoblastoma HepG2 cells

Sodium-dependent uptake of bile acids from blood is aliver-specific function which is mediated by theNa+-taurocholate cotransporting polypeptide(Ntcp). We report the stable expression of aNa+-taurocholate cotransporting green fluorescentfusion protein in the human hepatoblastoma cell lineHepG2, normally lacking Ntcp expression. Ntcp-EGFPassociated green fluorescence colocalized with Ntcpimmunofluorescence in the plasma membrane. Intransfected HepG2 cells, the fusion protein mediatedthe sodium-dependent uptake of the bile acidtaurocholate (Km: 24.6 μmol/l) and of the anionicsteroids estrone-3-sulfate and dehydroepiandrosteronesulfate. We conclude that the Ntcp-EGFP fusion proteinfollows the sorting route of Ntcp, is functionallyidentical to Ntcp and could be used to monitor proteintrafficking in living HepG2 cell

Significance of Coprophagy for the Fatty Acid Profile in Body Tissues of Rabbits Fed Different Diets

Four groups of eight New Zealand hybrid rabbits were fattened with ad libitum access to the following pelleted experimental diets: ryegrass meal or alfalfa meal fed either alone or with oats meal in a ratio of 1:1. After 25weeks they were slaughtered and dissected. Fatty acid (FA) profiles of caecotrophs (re-ingested fermentation products of the caecum), perirenal adipose tissue and intramuscular fat in the Musculus quadriceps were determined. With high proportions of branched-chain FA (BFA) and trans FA, and increased proportions of saturated FA relative to the diets, the caecotroph FA profile showed a clear fingerprint of anaerobe microbial lipid metabolism including biohydrogenation. By contrast, the FA profiles of adipose and lean tissue comprised high proportions of polyunsaturated FA (PUFA), whilst BFA and trans FA occurred in much lower proportions compared to the caecotrophs. Thus, coprophagy did not substantially modify the FA composition of the tissues investigated. Use of forage-only diets, compared to the oats supplemented diets, led to extraordinary high proportions of n-3 PUFA (including 18:3 and long-chain n-3) in the fat of adipose (21.3 vs. 6.7%) and lean tissue (15.4 vs. 5.7%). The forage type diet (grass vs. alfalfa) had smaller effects on the FA profiles. Indications of diet effects on endogenous desaturation, chain elongation and differential distribution of functional FA between the two tissues investigated were foun

BLUF Domain Function Does Not Require a Metastable Radical Intermediate State

BLUF (blue light using flavin) domain proteins are an important family of blue light-sensing proteins which control a wide variety of functions in cells. The primary light-activated step in the BLUF domain is not yet established. A number of experimental and theoretical studies points to a role for photoinduced electron transfer (PET) between a highly conserved tyrosine and the flavin chromophore to form a radical intermediate state. Here we investigate the role of PET in three different BLUF proteins, using ultrafast broadband transient infrared spectroscopy. We characterize and identify infrared active marker modes for excited and ground state species and use them to record photochemical dynamics in the proteins. We also generate mutants which unambiguously show PET and, through isotope labeling of the protein and the chromophore, are able to assign modes characteristic of both flavin and protein radical states. We find that these radical intermediates are not observed in two of the three BLUF domains studied, casting doubt on the importance of the formation of a population of radical intermediates in the BLUF photocycle. Further, unnatural amino acid mutagenesis is used to replace the conserved tyrosine with fluorotyrosines, thus modifying the driving force for the proposed electron transfer reaction; the rate changes observed are also not consistent with a PET mechanism. Thus, while intermediates of PET reactions can be observed in BLUF proteins they are not correlated with photoactivity, suggesting that radical intermediates are not central to their operation. Alternative nonradical pathways including a keto–enol tautomerization induced by electronic excitation of the flavin ring are considered

Implementing antisemitism studies in German teacher education

The integration of antisemitism as a topic for teacher education is urgent, especially in the social sciences Education regarding contemporary antisemitism must be combined with an analysis of racism and historical-political perspectives on the Shoah to succeed in post-migrant society Slight changes of the curricula are a reliable base for further development A nexus between schools, universities and actors of civil society is needed in order to target antisemitism International summer schools, German-Israeli study groups or collaborative workshops are exemplary pilot projects in order to establish competence regarding education critical of antisemitism   Purpose: Following the current rise of antisemitism globally and in Germany, this paper examines the current situation regarding Antisemitism in teacher education. The paper aims to make a contribution for the demand of an implementation of education critical of Antisemitism in teacher education. Approach: Combining experience from university teacher training and the field of extracurricular political education we appoint perspectives for a sustainable implementation of education critical of antisemitism in teacher training via best practice examples. We evaluate the potentials of field trips, international study groups and networks between university and agents located in civil society. Findings: The revised curricula for Social Sciences in the state of North-Rhine-Westphalia (and formerly Berlin) name current antisemitism for the first time, indicating an urgent need for professionalization in Social Science teacher education where the facets of antisemitism only play a minor role. Hence, expertise and existing resource must be stabilized, a structural implementation and funding is needed in order to target the challenging topic of antisemitism. Research limitations/implications: This paper functions as a preliminary research in order to examine the given shape of Social Science teacher education and Antisemitism in Germany. A comprehensive, structured analyses of all teacher training facilities as well as extracurricular agents working in the field of education critical of antisemitism could be useful in order to pool expertise. Nonetheless this paper encourages local collaborations between university and civiliety actors

Recipes for spin-based quantum computing

Technological growth in the electronics industry has historically been measured by the number of transistors that can be crammed onto a single microchip. Unfortunately, all good things must come to an end; spectacular growth in the number of transistors on a chip requires spectacular reduction of the transistor size. For electrons in semiconductors, the laws of quantum mechanics take over at the nanometre scale, and the conventional wisdom for progress (transistor cramming) must be abandoned. This realization has stimulated extensive research on ways to exploit the spin (in addition to the orbital) degree of freedom of the electron, giving birth to the field of spintronics. Perhaps the most ambitious goal of spintronics is to realize complete control over the quantum mechanical nature of the relevant spins. This prospect has motivated a race to design and build a spintronic device capable of complete control over its quantum mechanical state, and ultimately, performing computations: a quantum computer. In this tutorial we summarize past and very recent developments which point the way to spin-based quantum computing in the solid-state. After introducing a set of basic requirements for any quantum computer proposal, we offer a brief summary of some of the many theoretical proposals for solid-state quantum computers. We then focus on the Loss-DiVincenzo proposal for quantum computing with the spins of electrons confined to quantum dots. There are many obstacles to building such a quantum device. We address these, and survey recent theoretical, and then experimental progress in the field. To conclude the tutorial, we list some as-yet unrealized experiments, which would be crucial for the development of a quantum-dot quantum computer.Comment: 45 pages, 12 figures (low-res in preprint, high-res in journal) tutorial review for Nanotechnology; v2: references added and updated, final version to appear in journa

Are sketch-and-precondition least squares solvers numerically stable?

Sketch-and-precondition techniques are popular for solving large least squares (LS) problems of the form $Ax=b$ with $A\in\mathbb{R}^{m\times n}$ and $m\gg n$. This is where $A$ is ``sketched" to a smaller matrix $SA$ with $S\in\mathbb{R}^{\lceil cn\rceil\times m}$ for some constant $c>1$ before an iterative LS solver computes the solution to $Ax=b$ with a right preconditioner $P$, where $P$ is constructed from $SA$. Popular sketch-and-precondition LS solvers are Blendenpik and LSRN. We show that the sketch-and-precondition technique is not numerically stable for ill-conditioned LS problems. Instead, we propose using an unpreconditioned iterative LS solver on $(AP)y=b$ with $x=Py$ when accuracy is a concern. Provided the condition number of $A$ is smaller than the reciprocal of the unit round-off, we show that this modification ensures that the computed solution has a comparable backward error to the iterative LS solver applied to a well-conditioned matrix. Using smoothed analysis, we model floating-point rounding errors to provide a convincing argument that our modification is expected to compute a backward stable solution even for arbitrarily ill-conditioned LS problems.Comment: 22 page

Fundamental accuracy-resolution trade-off for timekeeping devices

From a thermodynamic point of view, all clocks are driven by irreversible processes. Additionally, one can use oscillatory systems to temporally modulate the thermodynamic flux towards equilibrium. Focusing on the most elementary thermalization events, this modulation can be thought of as a temporal probability concentration for these events. There are two fundamental factors limiting the performance of clocks: On the one level, the inevitable drifts of the oscillatory system, which are addressed by finding stable atomic or nuclear transitions that lead to astounding precision of today's clocks. On the other level, there is the intrinsically stochastic nature of the irreversible events upon which the clock's operation is based. This becomes relevant when seeking to maximize a clock's resolution at high accuracy, which is ultimately limited by the number of such stochastic events per reference time unit. We address this essential trade-off between clock accuracy and resolution, proving a universal bound for all clocks whose elementary thermalization events are memoryless.Comment: 5 + 7 pages, 8 figures, published versio

Are sketch-and-precondition least squares solvers numerically stable?

Sketch-and-precondition techniques are efficient and popular for solving large least squares (LS) problems of the form ⁢= with ∈ℝ× and ≫. This is where is “sketched” to a smaller matrix ⁢ with ∈ℝ⌈⁢⌉× for some constant >1 before an iterative LS solver computes the solution to ⁢= with a right preconditioner , where is constructed from ⁢. Prominent sketch-and-precondition LS solvers are Blendenpik and LSRN. We show that the sketch-and-precondition technique in its most commonly used form is not numerically stable for ill-conditioned LS problems. For provable and practical backward stability and optimal residuals, we suggest using an unpreconditioned iterative LS solver on (⁢)⁢= with =⁢. Provided the condition number of is smaller than the reciprocal of the unit roundoff, we show that this modification ensures that the computed solution has a backward error comparable to the iterative LS solver applied to a well-conditioned matrix. Using smoothed analysis, we model floating-point rounding errors to argue that our modification is expected to compute a backward stable solution even for arbitrarily ill-conditioned LS problems. Additionally, we provide experimental evidence that using the sketch-and-solve solution as a starting vector in sketch-and-precondition algorithms (as suggested by Rokhlin and Tygert in 2008) should be highly preferred over the zero vector. The initialization often results in much more accurate soluti