Heterogeneity of wheat endosperm proteolipids (CM proteins)

Proteins extracted with CHCl3-MeOH from wheat endosperm have been fractionated by Sephadex G-100 and the 15 000–20 000 MW range fraction, designated CM protein, has been examined by combined electrofocusing (pH range 5–8) and electrophoresis (pH 3.2) and the heterogeneity of the electrophoretic components has been ascertained. It has been shown by joint mapping and by sequential extraction that CM proteins are extracted by 70% EtOH but not by H2O, although they can be made water-soluble after dialysis against an acid buffer, pH 3.2, 3 M urea, without losing their solubility in CHCl3-MeOH mixtures. It is concluded that CM proteins fit the definition of a Folch—Lees proteolipid. The Triticum aestivum (genomes ABD) map can be reconstructed by mixing T. durum (AB) and Aegilops squarrosa (D). The low intragenomic variability of CM protein is confirmed

Extreme relativistic electron fluxes at geosynchronous orbit: Analysis of GOES E > 2 MeV electrons

Relativistic electrons (E > 1 MeV) cause internal charging on satellites and are an important space weather hazard. A key requirement in space weather research concerns extreme events and knowledge of the largest flux expected to be encountered over the lifetime of a satellite mission. This is interesting both from a scientific and practical point of view since satellite operators, engineers and the insurance industry need this information to better evaluate the effects of extreme events on their spacecraft. Here we conduct an extreme value analysis of daily averaged E > 2 MeV electron fluxes from the Geostationary Operational Environmental Satellites (GOES) during the 19.5 year period from 1 January 1995 to 30 June 2014. We find that the daily averaged flux measured at GOES West is typically a factor of ~2.5 higher than that measured at GOES East and we conduct independent analyses for these two locations. The 1 in 10, 1 in 50 and 1 in 100 year daily averaged E > 2 MeVelectron fluxes at GOES West are 1.84×105, 5.00×105 and 7.68×105 cm−2s−1sr−1 respectively. The corresponding fluxes at GOES East are 6.53×104, 1.98×105 and 3.25×105 cm−2s−1sr−1 respectively. The largest fluxes seen during the 19.5 year period on 29 July 2004 were particularly extreme and were seen by satellites at GOES West and GOES East. The extreme value analysis suggests that this event was a 1 in 50 year event

Evaluation of TypeSeq, a Novel High-Throughput, Low-Cost, Next-Generation Sequencing-Based Assay for Detection of 51 Human Papillomavirus Genotypes.

BackgroundHuman papillomaviruses (HPV) cause over 500 000 cervical cancers each year, most of which occur in low-resource settings. Human papillomavirus genotyping is important to study natural history and vaccine efficacy. We evaluated TypeSeq, a novel, next-generation, sequencing-based assay that detects 51 HPV genotypes, in 2 large international epidemiologic studies.MethodsTypeSeq was evaluated in 2804 cervical specimens from the Study to Understand Cervical Cancer Endpoints and Early Determinants (SUCCEED) and in 2357 specimens from the Costa Rica Vaccine Trial (CVT). Positive agreement and risks of precancer for individual genotypes were calculated for TypeSeq in comparison to Linear Array (SUCCEED). In CVT, positive agreement and vaccine efficacy were calculated for TypeSeq and SPF10-LiPA.ResultsWe observed high overall and positive agreement for most genotypes between TypeSeq and Linear Array in SUCCEED and SPF10-LiPA in CVT. There was no significant difference in risk of precancer between TypeSeq and Linear Array in SUCCEED or in estimates of vaccine efficacy between TypeSeq and SPF10-LiPA in CVT.ConclusionsThe agreement of TypeSeq with Linear Array and SPF10-LiPA, 2 well established standards for HPV genotyping, demonstrates its high accuracy. TypeSeq provides high-throughput, affordable HPV genotyping for world-wide studies of cervical precancer risk and of HPV vaccine efficacy

The main and accessory olfactory systems interact in the control of mate recognition and sexual behavior

Peer reviewe

The Bolocam Galactic Plane Survey IV: 1.1 and 0.35 mm Dust Continuum Emission in the Galactic Center Region

The Bolocam Galactic Plane Survey (BGPS) data for a six square degree region of the Galactic plane containing the Galactic center is analyzed and compared to infrared and radio continuum data. The BGPS 1.1 mm emission consists of clumps interconnected by a network of fainter filaments surrounding cavities, a few of which are filled with diffuse near-IR emission indicating the presence of warm dust or with radio continuum characteristic of HII regions or supernova remnants. New 350 {\mu}m images of the environments of the two brightest regions, Sgr A and B, are presented. Sgr B2 is the brightest mm-emitting clump in the Central Molecular Zone and may be forming the closest analog to a super star cluster in the Galaxy. The Central Molecular Zone (CMZ) contains the highest concentration of mm and sub-mm emitting dense clumps in the Galaxy. Most 1.1 mm features at positive longitudes are seen in silhouette against the 3.6 to 24 {\mu}m background observed by the Spitzer Space Telescope. However, only a few clumps at negative longitudes are seen in absorption, confirming the hypothesis that positive longitude clumps in the CMZ tend to be on the near-side of the Galactic center, consistent with the suspected orientation of the central bar in our Galaxy. Some 1.1 mm cloud surfaces are seen in emission at 8 {\mu}m, presumably due to polycyclic aromatic hydrocarbons (PAHs). A ~0.2\degree (~30 pc) diameter cavity and infrared bubble between l \approx 0.0\degree and 0.2\degree surrounds the Arches and Quintuplet clusters and Sgr A. The bubble contains several clumpy dust filaments that point toward Sgr A\ast; its potential role in their formation is explored. [abstract truncated]Comment: 76 pages, 22 figures, published in ApJ: http://iopscience.iop.org/0004-637X/721/1/137

SUNITINIB-INDUCED CARDIOTOXICITY EXPOSES MICROVASCULAR AND METABOLIC DERANGEMENTS IN THE FAILING HEART

Cardiac dysfunction is one of the largest obstacles to effective cancer treatment. Not only do a growing number of cancer patients have significant cardiovascular risk factors at the time of diagnosis, but a number of targeted therapies can themselves cause cardiac dysfunction. These newer treatment options are also increasingly effective, placing a growing emphasis on post-cancer quality of life. In some instances, a patient may outlive his or her cancer only to succumb to heart failure in as little as five years. One such targeted therapy is the small molecular receptor tyrosine kinase sunitinib. Sunitinib was developed in the early 2000’s as part of a wave of antiangiogenic drugs that target the vascular endothelial growth factor receptors and platelet-derived growth factor receptors. Unfortunately, up to 47% of patients treated with sunitinib develop severe hypertension and approximately 20% develop cardiac dysfunction. The aim of my dissertation is therefore twofold: First, to identify the mechanism of sunitinib-induced cardiotoxicity in order to develop strategies to prevent clinical cardiotoxicity and, secondly, to use this mechanistic insight as a discovery platform to identify new targets for the treatment of human heart failure. My work led to the discovery that coronary microvascular pericytes are a primary target of sunitinib. Furthermore, co-treatment with thalidomide can protect from sunitinib-induced cardiac dysfunction without altering the antitumor effect of the drug. Examination of sunitinib-induced metabolic changes revealed that sunitinib treatment leads to a profound increase in glucose uptake in the heart and induction of the M2 isoform of pyruvate kinase. Collectively, my work suggests that vessel normalization and the Warburg Effect may be novel targets for the treatment of human heart failure

Oceanography and life history predict contrasting genetic population structure in two Antarctic fish species

Understanding the key drivers of population connectivity in the marine environment is essential for the effective management of natural resources. Although several different approaches to evaluating connectivity have been used, they are rarely integrated quantitatively. Here, we use a ‘seascape genetics’ approach, by combining oceanographic modelling and microsatellite analyses, to understand the dominant influences on the population genetic structure of two Antarctic fishes with contrasting life histories, Champsocephalus gunnari and Notothenia rossii. The close accord between the model projections and empirical genetic structure demonstrated that passive dispersal during the planktonic early life stages is the dominant influence on patterns and extent of genetic structuring in both species. The shorter planktonic phase of C. gunnari restricts direct transport of larvae between distant populations, leading to stronger regional differentiation. By contrast, geographic distance did not affect differentiation in N. rossii, whose longer larval period promotes long-distance dispersal. Interannual variability in oceanographic flows strongly influenced the projected genetic structure, suggesting that shifts in circulation patterns due to climate change are likely to impact future genetic connectivity and opportunities for local adaptation, resilience and recovery from perturbations. Further development of realistic climate models is required to fully assess such potential impacts

Gestión del talento humano y rendimiento laboral en el personal de salud de un hospital II-1 La Libertad, 2024

La gestión del talento humano propicia el incremento del rendimiento laboral y representa una ventaja competitiva para las organizaciones, así esta investigación contribuye al objetivo de desarrollo sostenible 08, donde busca promover que las personas tengan el acceso a un empleo decente. El objetivo fue determinar en qué medida se relaciona la gestión del talento humano (GTH) y el rendimiento laboral (RL) del personal de salud de un Hospital II-1 de La Libertad, 2024. La metodología empleada fue de tipo aplicada, cuantitativa, no experimental y correlacional causal, con una muestra de 86 trabajadores, a quienes se les aplicó dos cuestionarios. Los resultados mostraron 46,5% refirió nivel regular de GTH, mientras que 54,7% tuvo buen RL, evidenciando asociación significativa, positiva de nivel bajo entre ambas variables, Rho 0,371 (p=<0,001); así también respecto a las dimensiones de GTH: selección y reclutamiento presentó un resultado de asociación de Rho de 0,324; seguido por capacitación y desarrollo con Rho de 0,366, y la dimensión de compensación Rho de 0,324. Se concluye que existe una correlación positiva y baja tanto entre la gestión del talento humano y sus dimensiones, respecto al rendimiento laboral del personal de salud de un Hospital II-1 de La Libertad, 2024

Assessing the Exchange of Knowledge Between Operations Management and other Fields: Some Challenges and Opportunities

Addressing a suspicion that the field of Operations Management (OM) draws substantially more knowledge from non-OM journals than those journals draw from OM journals in return, we studied the citations of the top 30 research journals of interest to our field. We conducted citation analyses of the three oldest OM journals over three decades in comparison to the 27 other journals representing the fields of Management, Operations Research/Management Science (OR/MS), Marketing, Practice, and Engineering. We examined both the entire 30-year period and then each decade separately. Our suspicions were confirmed—although citations from these 27 journals to these three OM journals have increased by a factor of 7 over the three decades, we in OM still cite these journals about twenty-five times more often than they cite our journals, giving an indication of the knowledge development and flows among these fields. We then describe some challenges for the field of OM in providing more research knowledge to other fields but also some opportunities that OM should be able to capitalize on, such as our historic ties to practice and our escalating research in strategic and organizational issues

Indiana community health workers: challenges and opportunities for workforce development

BackgroundAn interest in, and the need for, Community Health Workers (CHWs) in the United States is growing exponentially. CHWs possess a unique ability to relate to and build trust with communities in order to improve clinical outcomes, while building individual and community capacity. Given their critical role in addressing social determinants of health, expanding the CHW workforce is crucial. However, creating CHW jobs, facilitating training and certification, and establishing sustainable financing models to support this workforce has been challenging. MethodsA mixed-methods study consisting of an online survey and focus group discussions assessed the strengths, practices, and challenges to CHW workforce sustainability and expansion in the state of Indiana, including perspectives from both CHWs and employers. ResultsAcross 8 topics, mixed data analysis revealed 28 findings that were both complementary and unique across focus group and survey results. Results highlighted CHW skills and attributes, illustrated the recruitment and hiring process, and provided insight into measuring outcomes and outputs. Findings also indicated a need to build position validation, professional development, and billing and reimbursement capacity. ConclusionBuilding and sustaining the CHW workforce will require creating an evidence base of roles and impact, increasing awareness of existing reimbursement mechanisms, and sharing best practices across employer organizations to promote optimal recruitment, training, supervision, career development, and funding strategies