Human T cell glycosylation and implications on immune therapy for cancer

Glycosylation is an important post-translational modification, giving rise to a diverse and abundant repertoire of glycans on the cell surface, collectively known as the glycome. When focusing on immunity, glycans are indispensable in virtually all signaling and cell-cell interactions. More specifically, glycans have been shown to regulate key pathophysiological steps within T cell biology such as T cell development, thymocyte selection, T cell activity and signaling as well as T cell differentiation and proliferation. They are of major importance in determining the interaction of human T cells with tumor cells. In this review, we will describe the role of glycosylation of human T cells in more depth, elaborate on the importance of glycosylation in the interaction of human T cells with tumor cells and discuss the potential of cancer immunotherapies that are based on manipulating the glycome functions at the tumor immune interface

The contribution of maternal serum markers in the early prenatal diagnosis of molar pregnancies

The aim of this study was to evaluate the usefulness of maternal serum markers in the early prenatal diagnosis of molar pregnancies. The ultrasound features, cytogenetic and histopathological findings of 10 cases of molar pregnancy diagnosed at 11-13 weeks of gestation were compared retrospectively with the maternal serum concentrations of human chorionic gonadotrophin (HCG), alpha fetoprotein (AFP), pregnancy-associated plasma protein A (PAPP-A) and pregnancy-specific β1-glycoprotein (SP1). Free β-HCG and intact HCG concentrations were very high [≥ISOdia≥2.5 multiples of the median (MoM)] in all cases. AFP concentrations were extremely low in all cases of singleton complete moles (≤ISOdia≤0.5 MoM) and were high in one case of twin complete mole, in one case of triploid partial mole and two cases of euploid partial mole (≥ISOdia≥2.5 MoM). Serum PAPP-A and SP1 were high in complete moles. The combined use of ultrasound features, maternal serum proteins and fetal cytogenetic findings should enable the early differential diagnosis in utero and perinatal management of those molar pregnancies presenting with an anatomically normal fetu

Paracrine IL-2 Is Required for Optimal Type 2 Effector Cytokine Production

IL-2 is a pleiotropic cytokine that promotes the differentiation of Th cell subsets, including Th1, Th2, and Th9 cells, but it impairs the development of Th17 and T follicular helper cells. Although IL-2 is produced by all polarized Th subsets to some level, how it impacts cytokine production when effector T cells are restimulated is unknown. We show in this article that Golgi transport inhibitors (GTIs) blocked IL-9 production. Mechanistically, GTIs blocked secretion of IL-2 that normally feeds back in a paracrine manner to promote STAT5 activation and IL-9 production. IL-2 feedback had no effect on Th1- or Th17-signature cytokine production, but it promoted Th2- and Th9-associated cytokine expression. These data suggest that the use of GTIs results in an underestimation of the presence of type 2 cytokine-secreting cells and highlight IL-2 as a critical component in optimal cytokine production by Th2 and Th9 cells in vitro and in vivo

Low temperature mobility in hafnium-oxide gated germanium p-channel metal-oxide-semiconductor field-effect transistors

Effective mobility measurements have been made at 4.2 K on high performance high-k gated germanium p-type metal-oxide-semiconductor field effect transistors with a range of Ge/gate dielectric interface state densities. The mobility is successfully modelled by assuming surface roughness and interface charge scattering at the SiO2 interlayer/Ge interface. The deduced interface charge density is approximately equal to the values obtained from the threshold voltage and subthreshold slope measurements on each device. A hydrogen anneal reduces both the interface state density and the surface root mean square roughness by 20%

An effective formulation of coupled electromagnetic-TCAD simulation for extremely high frequency onward

This paper presents an effective formulation tailored for electromagnetic-technology computer-aided design coupled simulations for extremely-high-frequency ranges and beyond (> 50 GHz). A transformation of variables is exploited from the starting A-V formulation to the E-V formulation, combined with adopting the gauge condition as the equation for scalar potential. The transformation significantly reduces the cross-coupling between electric and magnetic systems at high frequencies, providing therefore much better convergence for iterative solution. The validation of such transformations is ensured through a careful analysis of redundancy in the coupled system and material properties. Employment of the advanced matrix permutation technique further alleviates the extra computational cost introduced by the variable transformation. Numerical experiments confirm the accuracy and efficiency of the proposed E-V formulation. © 2011 IEEE.published_or_final_versio

Hard X-ray polarimetry with Caliste, a high performance CdTe based imaging spectrometer

Since the initial exploration of soft gamma-ray sky in the 60's, high-energy celestial sources have been mainly characterized through imaging, spectroscopy and timing analysis. Despite tremendous progress in the field, the radiation mechanisms at work in sources such as neutrons stars and black holes are still unclear. The polarization state of the radiation is an observational parameter which brings key additional information about the physical process. This is why most of the projects for the next generation of space missions covering the tens of keV to the MeV region require a polarization measurement capability. A key element enabling this capability is a detector system allowing the identification and characterization of Compton interactions as they are the main process at play. The hard X-ray imaging spectrometer module, developed in CEA with the generic name of Caliste module, is such a detector. In this paper, we present experimental results for two types of Caliste-256 modules, one based on a CdTe crystal, the other one on a CdZnTe crystal, which have been exposed to linearly polarized beams at the European Synchrotron Radiation Facility. These results, obtained at 200-300 keV, demonstrate their capability to give an accurate determination of the polarization parameters (polarization angle and fraction) of the incoming beam. Applying a selection to our data set, equivalent to select 90 degrees Compton scattered interactions in the detector plane, we find a modulation factor Q of 0.78. The polarization angle and fraction are derived with accuracies of approximately 1 degree and 5%. The modulation factor remains larger than 0.4 when essentially no selection is made at all on the data. These results prove that the Caliste-256 modules have performances allowing them to be excellent candidates as detectors with polarimetric capabilities, in particular for future space missions.Comment: 17 pages, 14 figures, 2 tables in Experimental Astronomy, 201

The Microchannel X-ray Telescope on Board the SVOM Satellite

We present the Micro-channel X-ray Telescope (MXT), a new narrow-field (about 1{\deg}) telescope that will be flying on the Sino-French SVOM mission dedicated to Gamma-Ray Burst science, scheduled for launch in 2021. MXT is based on square micro pore optics (MPOs), coupled with a low noise CCD. The optics are based on a "Lobster Eye" design, while the CCD is a focal plane detector similar to the type developed for the seven eROSITA telescopes. MXT is a compact and light (<35 kg) telescope with a 1 m focal length, and it will provide an effective area of about 45 cmsq on axis at 1 keV. The MXT PSF is expected to be better than 4.2 arc min (FWHM) ensuring a localization accuracy of the afterglows of the SVOM GRBs to better than 1 arc min (90\% c.l. with no systematics) provided MXT data are collected within 5 minutes after the trigger. The MXT sensitivity will be adequate to detect the afterglows for almost all the SVOM GRBs as well as to perform observations of non-GRB astrophysical objects. These performances are fully adapted to the SVOM science goals, and prove that small and light telescopes can be used for future small X-ray missions.Comment: 6 pages, 6 figures, proceedings of the conference "Swift: 10 years of Discovery", Rome, December 2-5, 2014. To be published by Po