Qua Re qui possum non esse popularis: The representation of Populares in the Late Roman Republic.

The terms popularis and optimate have been employed in both ancient and modern literature to interpret late Roman Republican politics. The purpose of this work is to express the diversity and change of the popularis label from 133 to 88 B.C. as a consequence of developing political practices. A chronological assessment of five key popularis tribunes in this period; Ti. Sempronius Gracchus, G. Sempronius Gracchus, L. Appuleius Saturninus, M. Livius Drusus and P. Sulpicius Rufus determines the variation in political methodologies exploited by these men in response to an optimate opposition. An assessment of Cicero’s works then considers how the discrepancies exhibited by these politicians could be manipulated for oratorical advantage. This subsequently reveals the perception of pre-Sullan populares in the time of Cicero, a generation later. This work ultimately aims to demonstrate the individualistic nature of late Republican politicians, the evolution of political practice in the period and the diverse employment of political labels in contemporary sources

Ophyiulus in Victoria: results of millipede surveys from south-eastern Australia

The composition and ecology of the millipede fauna of Victoria remain poorly understood. We collected millipedes as part of a series of ecological arthropod surveys across south-eastern Australia, focusing mainly on Victoria. These samples almost exclusively contained millipedes from the introduced order Julida. We pursued species identification of the julids when it became apparent there were species other than the well-recorded Ommatoiulus moreleti (Lucas, 1860) (Portuguese millipede) in the samples. The majority of specimens were O. moreleti, but we also detected at least one species of Cylindroiulus Verhoeff, 1894, as well as an Ophyiulus Berlese, 1884, species, specimens of which have been identified as Ophyiulus cf. targionii. These are the first Ophyiulus records for Victoria to our knowledge. We present preliminary data on the abundance through the year of Ophyiulus. This is the first study to examine this species in Victoria and little is currently known about its likely impact on agriculture or on native species. Monitoring and research of the species in the future is therefore warranted

Biasing effects of receptor-ligand complexes on protein-unfolding statistics

Protein receptor-ligand pairs are increasingly used as specific molecular handles in single-molecule protein-unfolding experiments. Further, known marker domains, also referred to as fingerprints, provide unique unfolding signatures to identify specific single-molecule interactions, when receptor-ligand pairs themselves are investigated. We show here that in cases where there is an overlap between the probability distribution associated with fingerprint domain unfolding and that associated with receptor-ligand dissociation, the experimentally measured force distributions are mutually biased. This biasing effect masks the true parameters of the underlying free energy landscape. To address this, we present a model-free theoretical framework that corrects for the biasing effect caused by such overlapping distributions

Post-translational Sortase-mediated Attachment of High-strength Force Spectroscopy Handles

Single-molecule force spectroscopy greatly benefits from site-specific surface immobilization and specific probing with a functionalized cantilever. Here, we describe a streamlined approach to such experiments by covalently attaching mechanically stable receptors onto proteins of interest (POI) to improve pickup efficiency and specificity. This platform provides improved throughput, allows precise control over the pulling geometry, and allows for multiple constructs to be probed with the same ligand-modified cantilever. We employ two orthogonal enzymatic ligation reactions [sortase and phosphopantetheinyl transferase (Sfp)] to covalently immobilize POI to a pegylated surface and to subsequently ligate the POI to a mechanically stable dockerin domain at the protein’s C-terminus for use as a high-strength pulling handle. Our configuration permits expression and folding of the POI to proceed independently from the mechanically stable receptor used for specific probing and requires only two short terminal peptide sequences (i.e., ybbR-tag and sortase C-tag). We applied this system successfully to proteins expressed using in vitro transcription and translation reactions without a protein purification step and to purified proteins expressed in Escherichia coli

National Register of Historic Places Eligibility Testing of Site 41SM385 Within TxDOT\u27s Tyler District, Smith County, Texas

PBS&J, an Atkins company, was contracted by the North East Texas Regional Mobility Authority to conduct National Register of Historic Places eligibility testing of site 41SM385, a prehistoric campsite on a small rise above the floodplain of Indian Creek in western Smith County, Texas. Testing investigations were conducted during March and September 2009. The site was subjected to a systematic program of shovel testing, mechanical trenching, and hand excavation in an effort to identify cultural features or living surfaces and optimize recovery of diagnostic faunal, floral, and artifactual remains. The recovered cultural artifacts indicate that site 41SM385 represents a probable Woodland and Caddo‐aged occupation on a small rise on the creek floodplain. The Woodland component is based on recovered small Gary and Kent projectile points characteristic of Woodland culture of the region. The Caddo component is based on ceramic sherds of probable Early or Middle Caddo origin identified at the site. Radiocarbon dating of four ceramic sherds supports these assessments with three sherds dating to the Early to Middle Caddo periods and one sherd dating to the Woodland period. The lack of identified cultural features suggests that the Woodland component probably represents a series of ephemeral usages of the location, probably as short‐term campsites. The Caddo‐aged artifacts at the site probably represent a series of ephemeral usage of the location, either as a resource procurement locus ancillary to nearby site 41SM404 or as a short‐term campsite. The testing program failed to locate living surfaces or cultural features containing in situ artifactual or organic remains preserved on the site. The absence of cultural features and the paucity of lithic tools or ceramic remains make more‐meaningful functional interpretation infeasible. For this reason, the site lacks the data resources that would warrant National Register of Historic Places isting or designation as a State Archeological Landmark. No further work is recommended

Automated CT and MRI Liver Segmentation and Biometry Using a Generalized Convolutional Neural Network.

PurposeTo assess feasibility of training a convolutional neural network (CNN) to automate liver segmentation across different imaging modalities and techniques used in clinical practice and apply this to enable automation of liver biometry.MethodsWe trained a 2D U-Net CNN for liver segmentation in two stages using 330 abdominal MRI and CT exams acquired at our institution. First, we trained the neural network with non-contrast multi-echo spoiled-gradient-echo (SGPR)images with 300 MRI exams to provide multiple signal-weightings. Then, we used transfer learning to generalize the CNN with additional images from 30 contrast-enhanced MRI and CT exams.We assessed the performance of the CNN using a distinct multi-institutional data set curated from multiple sources (n = 498 subjects). Segmentation accuracy was evaluated by computing Dice scores. Utilizing these segmentations, we computed liver volume from CT and T1-weighted (T1w) MRI exams, and estimated hepatic proton- density-fat-fraction (PDFF) from multi-echo T2*w MRI exams. We compared quantitative volumetry and PDFF estimates between automated and manual segmentation using Pearson correlation and Bland-Altman statistics.ResultsDice scores were 0.94 ± 0.06 for CT (n = 230), 0.95 ± 0.03 (n = 100) for T1w MR, and 0.92 ± 0.05 for T2*w MR (n = 169). Liver volume measured by manual and automated segmentation agreed closely for CT (95% limit-of-agreement (LoA) = [-298 mL, 180 mL]) and T1w MR (LoA = [-358 mL, 180 mL]). Hepatic PDFF measured by the two segmentations also agreed closely (LoA = [-0.62%, 0.80%]).ConclusionsUtilizing a transfer-learning strategy, we have demonstrated the feasibility of a CNN to be generalized to perform liver segmentations across different imaging techniques and modalities. With further refinement and validation, CNNs may have broad applicability for multimodal liver volumetry and hepatic tissue characterization

Strong partnerships make good partners: Insights about physician-hospital relationships from a study of physician executives

While physicians are likely to respond favorably in concept to hospital-based disease management and other clinical programs, they are less likely to accept their structural and functional characteristics. Because of their role at the hospital-physician interface, hospital physician executives are often tasked with implementing such programs. Given the challenges involved, a deeper understanding of the role of these executives in building the hospital-physician relationship will therefore be an important contribution. To this end, we surveyed senior physician executives at hospitals and health systems (n = 326), to assess their view of the hospital-physician relationship at their institutions, focusing especially on the role of medical staff cohesion. This article presents several of our key findings, in particular that (1) many physician executives identified their medical staff as having relatively low cohesion and (2) the perceived level of medical staff cohesion correlated strongly with the level of physician support for organizational priorities, the degree of constructive physician involvement, and success in improving the physician-hospital relationship. In light of these findings, we conclude by offering concrete recommendations for physician executives and others seeking to build medical staff cohesion in the service of clinical improvement

Effects of risperidone on cognitive-motor performance and motor movements in chronically medicated children

This study was designed to explore the placebo-controlled effects of risperidone on cognitive-motor processes, dyskinetic movements, and behavior in children receiving maintenance risperidone therapy. Sixteen children aged 4-14 years with disruptive behavior were randomly assigned to drug order in a crossover study of risperidone and placebo for 2 weeks each. Dependent measures included tests of sustained attention, memory, visual matching, tremor, seat activity, abnormal movements, and parent behavior ratings. Results were compared by repeated measures ANOVA Fourteen boys and 2 girls with disruptive behavior and IQ ≤ 84 all completed the protocol. Risperidone was superior to placebo on response time (p = 0.01, ηP ^ 2 = 0.43) and seat movement (p < 0.05, ηP ^ 2 = 0.29) on a short-term memory task, and on a measure of static tremor (p = 0.05, ηP ^ 2 = 0.28). There was not a significant difference between treatment conditions on the Abnormal Involuntary Movement scale. Risperidone was superior to placebo on three subscales of the Nisonger Child Behavior Rating Form [Overly Sensitive (p < 0.01, ηP ^ 2 = 0.44), Conduct Problem (p = 0.02, ηP ^ 2 = 0.36), Hyperactivity (p = 0.03, ηP ^ 2 = 0.32)] and on the Hyperactivity/Noncompliance subscale of the Aberrant Behavior Checklist (p = 0.01, ηP ^ 2 = 0.41). Significant increases in heart rate (p = 0.05, ηP ^ 2 = 0.27) and weight (p = 0.02, ηP ^ 2 = 0.36) occurred in the risperidone condition. The findings suggest a beneficial effect of risperidone after several months of treatment on efficiency of responding, activity level, static tremor, and aspects of behavior