Investigation into the binding characteristics of plant and animal heterotrimeric G protein α subunits with hexacoordinate hemoglobins

Heterotrimeric G proteins are a class of signaling molecules found throughout the animal kingdom with an extremely high degree of complexity and regulation. These molecules have just recently been discovered in plants as well, albeit with an incredible lack of intricacies. Elsewhere, it has been reported that neuroglobin, a newly discovered hexacoordinate hemoglobin, interacts with the Gα subunit in animal systems. Plants as well have many hexacoordinate hemoglobins, termed nonsymbiotic hemoglobins, of unknown function. Because of the similarity between plant and animal Gα subunits, the assumption that these plant nonsymbiotic hemoglobins may play a similar role with plant Gα subunits was investigated. Using purified atGPA1 from Arabidopsis and Gα[subscript il] from rat, the binding to multiple hemoglobins was measured with surface plasmon resonance. Both Gα subunits interact similarly with hemoglobins from both animal and plant kingdoms; however, animal hemoglobins exhibit a strong nucleotide dependence while plant hemoglobins do not. Mutations near the E7L histidine also play a role in binding. These novel findings lay the groundwork for future study on Gα and hemoglobin interactions

The FANCM:p.Arg658* truncating variant is associated with risk of triple-negative breast cancer

Abstract: Breast cancer is a common disease partially caused by genetic risk factors. Germline pathogenic variants in DNA repair genes BRCA1, BRCA2, PALB2, ATM, and CHEK2 are associated with breast cancer risk. FANCM, which encodes for a DNA translocase, has been proposed as a breast cancer predisposition gene, with greater effects for the ER-negative and triple-negative breast cancer (TNBC) subtypes. We tested the three recurrent protein-truncating variants FANCM:p.Arg658*, p.Gln1701*, and p.Arg1931* for association with breast cancer risk in 67,112 cases, 53,766 controls, and 26,662 carriers of pathogenic variants of BRCA1 or BRCA2. These three variants were also studied functionally by measuring survival and chromosome fragility in FANCM−/− patient-derived immortalized fibroblasts treated with diepoxybutane or olaparib. We observed that FANCM:p.Arg658* was associated with increased risk of ER-negative disease and TNBC (OR = 2.44, P = 0.034 and OR = 3.79; P = 0.009, respectively). In a country-restricted analysis, we confirmed the associations detected for FANCM:p.Arg658* and found that also FANCM:p.Arg1931* was associated with ER-negative breast cancer risk (OR = 1.96; P = 0.006). The functional results indicated that all three variants were deleterious affecting cell survival and chromosome stability with FANCM:p.Arg658* causing more severe phenotypes. In conclusion, we confirmed that the two rare FANCM deleterious variants p.Arg658* and p.Arg1931* are risk factors for ER-negative and TNBC subtypes. Overall our data suggest that the effect of truncating variants on breast cancer risk may depend on their position in the gene. Cell sensitivity to olaparib exposure, identifies a possible therapeutic option to treat FANCM-associated tumors

Perceived influence of proenvironmental testimonials

Recommendations for communicators to make environmental issues more concrete in public align with the tenets of exemplification theory. Audiences may also engage with messages that they perceive as influencing them more than others, an outcome that aligns with the third-person effects framework. What is not well known is how these two areas of research intersect, namely, how exemplars about environmental issues may impact perceived message influence on the self-relative to others. This study examines the effects of testimonials on the perceived influence of environmental messages. Two experiments, each conducted simultaneously in Singapore and the Midwestern US, suggest that university students perceive themselves to be more influenced than others by proenvironmental messages. The second experiment shows that this perceptual bias is related to message desirability and individuals’ environmental values. Both experiments reveal location-specific effects, which is useful for understanding how to communicate environmental problems to global audiences.Accepted versio

Third-person perception of science narratives : the case of climate change denial

Science communicators are increasingly recognizing the potential of narratives to reach and influence audiences. However, do audiences recognize and consider this tactic when evaluating how such messages influence themselves and others? This study compares third-person perceptions of persuasive narrative and nonnarrative messages in a climate change context. Results suggest that individuals are aware of the influence of narratives and are able to resist this influence, but this is only when they perceive a message as having negative influence. Otherwise, individuals underestimate the influence of narratives on themselves. These findings add an audience-centered perspective to the current discussions on incorporating narratives within science communication

Third-Person Effects Of Pro-Environmental Narratives

This was a study of proenvironmental narrative

The FANCM:p.Arg658* truncating variant is associated with risk of triple-negative breast cancer

AbstractBreast cancer is a common disease partially caused by genetic risk factors. Germline pathogenic variants in DNA repair genes BRCA1, BRCA2, PALB2, ATM, and CHEK2 are associated with breast cancer risk. FANCM, which encodes for a DNA translocase, has been proposed as a breast cancer predisposition gene, with greater effects for the ER-negative and triple-negative breast cancer (TNBC) subtypes. We tested the three recurrent protein-truncating variants FANCM:p.Arg658*, p.Gln1701*, and p.Arg1931* for association with breast cancer risk in 67,112 cases, 53,766 controls, and 26,662 carriers of pathogenic variants of BRCA1 or BRCA2. These three variants were also studied functionally by measuring survival and chromosome fragility in FANCM−/− patient-derived immortalized fibroblasts treated with diepoxybutane or olaparib. We observed that FANCM:p.Arg658* was associated with increased risk of ER-negative disease and TNBC (OR = 2.44, P = 0.034 and OR = 3.79; P = 0.009, respectively). In a country-restricted analysis, we confirmed the associations detected for FANCM:p.Arg658* and found that also FANCM:p.Arg1931* was associated with ER-negative breast cancer risk (OR = 1.96; P = 0.006). The functional results indicated that all three variants were deleterious affecting cell survival and chromosome stability with FANCM:p.Arg658* causing more severe phenotypes. In conclusion, we confirmed that the two rare FANCM deleterious variants p.Arg658* and p.Arg1931* are risk factors for ER-negative and TNBC subtypes. Overall our data suggest that the effect of truncating variants on breast cancer risk may depend on their position in the gene. Cell sensitivity to olaparib exposure, identifies a possible therapeutic option to treat FANCM-associated tumors.</jats:p

The FANCM:p.Arg658* truncating variant is associated with risk of triple-negative breast cancer

Breast cancer is a common disease partially caused by genetic risk factors. Germline pathogenic variants in DNA repair genes BRCA1, BRCA2, PALB2, ATM, and CHEK2 are associated with breast cancer risk. FANCM, which encodes for a DNA translocase, has been proposed as a breast cancer predisposition gene, with greater effects for the ER-negative and triple-negative breast cancer (TNBC) subtypes. We tested the three recurrent protein-truncating variants FANCM:p.Arg658*, p.Gln1701*, and p.Arg1931* for association with breast cancer risk in 67,112 cases, 53,766 controls, and 26,662 carriers of pathogenic variants of BRCA1 or BRCA2. These three variants were also studied functionally by measuring survival and chromosome fragility in FANCM−/− patient-derived immortalized fibroblasts treated with diepoxybutane or olaparib. We observed that FANCM:p.Arg658* was associated with increased risk of ER-negative disease and TNBC (OR = 2.44, P = 0.034 and OR = 3.79; P = 0.009, respectively). In a country-restricted analysis, we confirmed the associations detected for FANCM:p.Arg658* and found that also FANCM:p.Arg1931* was associated with ER-negative breast cancer risk (OR = 1.96; P = 0.006). The functional results indicated that all three variants were deleterious affecting cell survival and chromosome stability with FANCM:p.Arg658* causing more severe phenotypes. In conclusion, we confirmed that the two rare FANCM deleterious variants p.Arg658* and p.Arg1931* are risk factors for ER-negative and TNBC subtypes. Overall our data suggest that the effect of truncating variants on breast cancer risk may depend on their position in the gene. Cell sensitivity to olaparib exposure, identifies a possible therapeutic option to treat FANCM-associated tumors