A Sarcoplasmic Reticulum Localized Protein Phosphatase Regulates Phospholamban Phosphorylation and Promotes Ischemia Reperfusion Injury in the Heart.

Phospholamban (PLN) is a key regulator of sarcolemma calcium uptake in cardiomyocyte, its inhibitory activity to SERCA is regulated by phosphorylation. PLN hypophosphorylation is a common molecular feature in failing heart. The current study provided evidence at molecular, cellular and whole heart levels to implicate a sarcolemma membrane targeted protein phosphatase, PP2Ce, as a specific and potent PLN phosphatase. PP2Ce expression was elevated in failing human heart and induced acutely at protein level by β -adrenergic stimulation or oxidative stress in cardiomyocytes. PP2Ce expression in mouse heart blunted β-adrenergic response and exacerbated ischemia/reperfusion injury. Therefore, PP2Ce is a new regulator for cardiac function and pathogenesis

1-aminocyclopropane-1-carboxylate deaminase-producing Agrobacterium tumefaciens has higher ability for gene transfer into plant cells

Agrobacterium-mediated gene transfer is widely used for plant molecular genetics, and efficient techniques are required. Recent studies show that ethylene inhibits the gene transfer. To suppress ethylene evolution, we introduced 1-aminocyclopropane-1-carboxylate (ACC) deaminase into Agrobacterium tumefaciens. The ACC deaminase-producing A. tumefaciens showed higher ability of gene transfer in plants

Ethylene production in plants during transformation suppresses vir gene expression in Agrobacterium tumefaciens

•Ethylene evolution from plants inhibits Agrobacterium-mediated genetic transformation, but the mechanism is little understood. In this study, the possible role of ethylene in Agrobacterium-mediated genetic transformation was clarified.•It was tested whether or not plant ethylene sensitivity affected genetic transformation; the sensitivity might regulate bacterial growth during co-cultivation and vir gene expression in Agrobacterium tumefaciens. For these experiments, melon (Cucumis melo) was used, in which ethylene sensitivity was controlled by chemicals, and Arabidopsis ethylene-insensitive mutants.•Agrobacterium-mediated genetic transformation was inhibited in ethylene-sensing melon, whereas, in Arabidopsis ethylene-insensitive mutant, it was enhanced. However, the ethylene sensitivity did not affect bacterial growth. vir gene expression was inhibited by application of plant exudate from ethylene-sensitive plants. The inhibitory effect of the ethylene sensitivity on genetic transformation relieved the activation of vir gene expression in A. tumefaciens with vir gene inducer molecule (acetosyringone, AS) or A. tumefaciens mutant strain which has constitutive vir gene expression.•These results indicate that ethylene evolution from a plant inoculated with A. tumefaciens inhibited vir gene expression in A. tumefaciens through the ethylene signal transduction in the plant, and, as a result, Agrobacterium-mediated genetic transformation was inhibited

Conceptual metaphors for visual/auditory perception in Swedish

This study aims to illustrate how visual and auditory perception are conceptu­alized in Swedish and what differences there are between them. Previous studies often discuss perception in relation to the oppositely directed motions between the perceiver and the object perceived. In the Perceiver-as-Source type, perception occurs when our eyes/gaze reach the object perceived. In the Perceived-as-Source type, perception takes place when sense stimuli reach the perceiver. The data show two differences between visual and auditory perception. First, we find more metaphorical expressions for visual perception than for auditory perception. Second, we also find that, while visual perception has a stronger connection to the Perceiver-as-Source type, auditory perception is more strongly connected to the Perceived-as-Source type. These two differen­ces are explained by the function of the perceptual organs

Unique Phenotypes of Endothelial Cells in Developing Arteries: A Lesson from the Ductus Arteriosus

Endothelial cells (ECs) play a critical role in regulating vascular pathophysiology. Various growth factors and relaxation factors such as vascular endothelial growth factor (VEGF) and nitric oxide (NO), which are derived from ECs, are known to maintain homeostasis and regulate vessel remodeling. Although the inner lumens of all types of vessels are covered by an EC monolayer, the characteristics of ECs differ in each tissue and developing stage of a vessel. Previously, we identified the heterogeneity of ECs of the ductus arteriosus (DA) by analyzing its gene profiles. The DA is a fetal artery that closes immediately after birth due to the changes in concentrations of oxygen and vasoactive factors such as NO and prostaglandin E. Studying the unique gene profile of ECs in the DA can therefore uncover the novel key genes involved in developing vascular function and morphology such as O2 sensitivity and physiological vascular remodeling. A comprehensive gene analysis identified a number of genes related to morphogenesis and development in the DA. In this chapter, we discuss the heterogeneity of vascular ECs in the developing vessel in the DA

<Original Article> Age-Related Changes in the Spontaneous Motor Activity during the Dark Phase in the Male Mouse

Spontaneous motor activity was studied in first generation (F1) hybrid C57BL/6 x C3H male mice at the ages of 6, 18 and 36 months. Activity was recorded for 10 consecutive hours during the dark phase, using a capacitance-induction motility monitor. The recordings were taken simultaneously at 2 levels of sensitivity; total activity (T) for the fine and gross movements and large activity (L) for the gross movements. The data were measured in 1 hour intervals and then stored on discs for micro-computer analysis. The T activity decreased with age. Changes in the mean hourly scores of T and L activity with time during the 10 hour dark phase differed among the 3 age groups. The results suggest that spontaneous motor activity during the dark phase is a useful parameter of changes in the motor function in aged mice.journal articl