Advancing the cultural study of personality and identity: models, methods, and outcomes

Personality and identity are overlapping but distinct constructs. This article describes the three-level model of personality and, specifically, how identity fits within it. Importantly, identity operates at multiple levels of personality, depending on whether the focus is on domain-specific identity development processes or the integrated life story on a broad scale. The strength of the three-level model of personality is then described with respect to a) how it provides an ideal framework for cultural psychological inquiries into universality and specificity, b) how it is well suited for mixed methods research that combines quantitative and qualitative methods, and c) how different levels of personality may be related to psychological outcomes.Personality and identity are overlapping but distinct constructs. This article describes the three-level model of personality and, specifically, how identity fits within it. Importantly, identity operates at multiple levels of personality, depending on whether the focus is on domain-specific identity development processes or the integrated life story on a broad scale. The strength of the three-level model of personality is then described with respect to a) how it provides an ideal framework for cultural psychological inquiries into universality and specificity, b) how it is well suited for mixed methods research that combines quantitative and qualitative methods, and c) how different levels of personality may be related to psychological outcomes

Advancing the cultural study of personality and identity: models, methods, and outcomes

Personality and identity are overlapping but distinct constructs. This article describes the three-level model of personality and, specifically, how identity fits within it. Importantly, identity operates at multiple levels of personality, depending on whether the focus is on domain-specific identity development processes or the integrated life story on a broad scale. The strength of the three-level model of personality is then described with respect to a) how it provides an ideal framework for cultural psychological inquiries into universality and specificity, b) how it is well suited for mixed methods research that combines quantitative and qualitative methods, and c) how different levels of personality may be related to psychological outcomes

Sources of fear in American society: Representations in short horror fiction, 1950s-present

This study examines the ways in which short American horror fiction has always revolved around fundamental fears of mortality, and how these fears have shifted across the span of three specific timeframes. Using a historical lens, this study also explores what the specific nature of mortality fears, as reflected in particular instances of short horror fiction, historically reveal about contemporaneous cultural attitudes toward end of life issues, loss, doubt, and grief. This study also traces how the perceptions of mortality have dynamically changed in American society from 1950s to present times in accordance with powerful historical events, varying cultural contexts, and compelling social issues

Moving Finite Element Solution of Discontinuous Open Channel Flow

Over the years, rapidly varying channel flow and discontinuities in the solution of fluid mechanics problems have provided stimulation and challenge to numerical modelers. Traditional finite difference and finite element methods produce accurate but oscillatory solutions. Attempts to selectively eliminate these parasitic waves have been only partially successful in that the cost of a smoother profile was a lower accuracy solution. It is common to employ either internal and external dissipation parameters or a provision of dispersive interface. In this thesis, the problem of rapidly varying open channel flow is represented by a pair of nonlinear partial differential equations which are solved by a powerful moving finite element technique. The method developed in this research is based on the linking of a novel Lagrangian mode solution with the convenience of the Eularian grid at each time step. This second order scheme was employed in solving a variety of devised and reported open channel flow problems with near discontinuities. Comparisons with solutions obtained using the finite difference and finite element methods with Crank-Nicholson centred weightings demonstrates the quality improvements which have been achieved by this moving element scheme. The basic scheme was further generalized in both spatial and temporal dimensions. Sensitivity analysis of these generalized parameters established the grid size relaxations for a variety of problems. The moving element technique solved near discontinuous and gradually varied flow problems both in supercritical and subcritical regimes. An alternate form of Petrov-Galerkin weighting function was tested and found to give promising results. Further experimentation and testing are required before implementation. The robustness of the solution procedure is indicated by the adaptation of the model from the numerical and laboratory experiment stage to field problems. The model was successfully applied to the Teton Dam break flood and flood routing problem in the (Ontario) Grand River basin. Sensitivity analysis with very mild sloped channels with topographical features such as sudden expansions and offchannel storage suggest that the Eulerian-Lagrangian mode algorithm provides the missing link between the fluid mechanics of discontinuities and a practical tool for the modelling of rapidly varying open channel flow.Doctor of Philosophy (PhD

Identification of a Cryptic Pocket in Methionine Aminopeptidase-II Using Adaptive Bandit Molecular Dynamics Simulations and Markov State Models

Methionine aminopeptidase-II (MetAP-II) is a metalloprotease, primarily responsible for the cotranslational removal of the N-terminal initiator methionine from the nascent polypeptide chain during protein synthesis. MetAP-II has been implicated in angiogenesis and endothelial cell proliferation and is therefore considered a validated target for cancer therapeutics. However, there is no effective drug available against MetAP-II. In this study, we employ Adaptive Bandit molecular dynamics simulations to investigate the structural dynamics of the apo and ligand-bound MetAP-II. Our results focus on the dynamic behavior of the disordered loop that is not resolved in most of the crystal structures. Further analysis of the conformational flexibility of the disordered loop reveals a hidden cryptic pocket that is predicted to be potentially druggable. The network analysis indicates that the disordered loop region has a direct signaling route to the active site. These findings highlight a new way to target MetAP-II by designing inhibitors for the allosteric site within this disordered loop region

The hepatitis E virus Orf3 protein protects cells from mitochondrial depolarization and death

The biology and pathogenesis of hepatitis E virus are poorly understood due to the lack of an in vitro culture or infection models. The viral Orf3 protein activates the cellular mitogen-activated protein kinase pathway and is likely to modulate the host cell environment for efficient viral replication. We screened for cellular genes whose transcription was differentially up-regulated in an Orf3-expressing stable cell line (ORF3/4). The gene for mitochondrial voltage-dependent anion channel (VDAC) was one such candidate. The up-regulation of VDAC in ORF3/4 cells was confirmed by Northern and Western blotting in various cell lines. Transfection of ORF3/4 cells with an ORF3-specific small interfering RNA led to a reduction in VDAC protein levels. VDAC is a critical mitochondrial outer membrane protein, and its overexpression results in apoptosis. Surprisingly, Orf3-expressing cells were protected against staurosporine-induced cell death by preservation of mitochondrial potential and membrane integrity. A small interfering RNA-mediated reduction in Orf3 and VDAC levels also made cells sensitive to staurosporine. Chemical cross-linking showed Orf3-expressing cells to contain higher levels of oligomeric VDAC. These cells also contained higher levels of hexokinase I that directly interacted with VDAC. This interaction is known to preserve mitochondrial potential and prevent cytochrome c release. We report here the first instance of a viral protein promoting cell survival through such a mechanism

Azo dyes decolourisation by ABTS-oxidases (Laccases) from a fungus from tropical tree

Lignin is an important constitute of the wood and thus the organism like fungi and insects growing on wood produce some extracellular lignolytic enzymes. In fungi these enzymes include different oxidases and peroxidases. Lignin peroxidases, manganese peroxidases, versatile peroxidases and laccases have been characterised from fungi and their roles in wood decay has been established. Here we report ABTS-oxidases from an unidentified fungus isolated from tree bark sample from Kuantan, Malaysia. After the liquid media cultivation, the crude extracellular protein content was extracted and analysed by polyacrylamide gel electrophoresis. SDS-PAGE, native PAGE and zymography were carried out. Azo dyes, brilliant green, trypan blue, direct red 80 and eriochrome black T were used for decolourisation activity of the isolated fungus on solid medium. The results suggest that the extracellular proteins contain laccase enzyme activity. Out of the four azo dyes that were used for dye decolourisation assay, at least three were decolourised to different extents by the fungus

Examination of Psychometric Properties of the Need for Closure Scale-Short Form among Turkish College Students

Amaç: Bilişsel tamamlanma ihtiyacı (BTİ), son zamanlarda davranışsal nöro-bilimin önem kazanmaya başlayan konularından biridir ve bireyin belirli bir konuda karmaşa ve belirsizlik yerine kesin bir bilgiye ulaşma ihtiyacı, karar verme arzusudur. Bu çalışmanın amacı, tamamlanma ihtiyacını ölçen "Tamamlanma İhtiyacı Ölçeği-Kısa Formu'nun" Türk üniversite öğrencilerinde uygunluğunu incelemektirIntroduction: The need for cognitive closure (NFC), defined as the desire for “an answer on a given topic, any answer … compared to confusion and ambiguity,” is a topic that has become increasingly important in behavioral neuroscience. The present study aimed to assess the applicability of a measure of need for cognitive closure, the “Need for Closure Scale-Short Form” (NFC-SF), for Turkish college students.Methods: Data from a total of 219 participants aged 19–29 years (male n=61, 27.9%; female n=158, 72.1%; Meanage=23.6 years, SD=3.06 years) were used to conduct validity and reliability analyses. Of the participants, 138 (63.0%; Meanage=22.62 years; SD=2.45 years) were university students, and the remainder had graduated from university (n=81; 37%; Meanage=24.16 years; SD=3.24 years

A Study on the Contributing Factors of Major Landslides in Malaysia

Landslide is one of the most prominent geo-hazard that accounts for colossal losses every year. The contributing factors of landslides in Malaysia are reasonably different from rest of the world. According to a study, the most dominant factor that catalysis the slope failure in many countries of the world is geological conditions. However, in case of Malaysia; most of the landslides occur as a consequence of flawed design, improper construction and non-maintenance of slopes which correlates with the human errors. The statistics of Malaysia shows that highest number of landslides took place in 1996 with 71 cases which is followed by 68 cases in 1995. According to the findings of Highland Towers (1993) landslide, the main causes of failure were inaccuracies in design, poor supervision during the construction and inadequate drainage system. Similarly, in case of Taman Hillview and Bukit Antarabangsa landslide, it has been revealed that improper design practices and poor drainage system supplemented with prolonged rain were the significant causes of the cataclysm. Therefore, based on the investigations on retrospective landslides, this study proposes to incorporate Human Reliability Assessment technique as a part of working strategy for slopes along with strong adherence to the design guidelines in order to minimize the likelihood of landslides

In Vitro Antioxidant and In Vivo Hepatoprotective Activity of Leave Extract of Raphanus Sativus in Rats Using CCL4 Model

Background: Raphanus sativus is reported to have a variety of biological activities. This work screened the hepato-protective and antioxidant activity of ethanol (ERS), and aqueous (ARS), extracts of leaves of Raphanus sativus in Carbon tetrachloride (CCl4), model in rats.Material and Methods: The extracts were subjected to antioxidant tests (Total reducing power and Total phenolic content), and preliminary phytochemical screening. A pilot study was done on 100 and 300 mg/kg extracts, form which 300 mg was chosen for further experiments. The albino rats (200-250 grams), were divided into 5 groups of 6 animals each (n=6). There were three control groups comprising of normal control (normal saline -1ml/kg), negative control group (CCl4 1ml/kg in olive oil in a ratio of 1:1 v/v), and positive control group (Silymarin 50mg/kg). The Test drugs were given in a dose of 300 mg/kg for both ERS and ARS extract for 7 days. Biochemical parameters (AST, ALT, Alkaline phosphatase, Total Bilirubin), histo-pathological examination of liver and in vivo antioxidant tests [CAT, GSH and MDA] were done.Results: The phytochemical study showed the presence of flavanoids, terpenoids, alkaloids, saponins and sterols. A dose dependent increase in the oxidative potential was observed in both the extracts with total phenolic content 70.1 and 44.4 GAE/g extract for ERS and ARS respectively. ERS 300mg/kg showed a significant (p<0.001) increase in levels of AST, ALT and alkaline phosphatase as compared to negative control (percentage hepatoprotection =45.3%) while ARS 300 mg/kg (p<.01) group showed 30% hepatoprotection. The GSH (p<0.001) and CAT (p<0.05) in ERS and ARS were significantly increased while MDA levels were decreased (P< 0.01), as compared negative control. The findings were confirmed histo-pathological examination.Conclusion: The ethanol and aqueous extract of Raphanus sativus have partial hepatoprotection against CCl4 toxicity.Key words: Raphanus sativus, hepatoprotection, CCl4, antioxidant