Dependable Distributed Computing for the International Telecommunication Union Regional Radio Conference RRC06

The International Telecommunication Union (ITU) Regional Radio Conference (RRC06) established in 2006 a new frequency plan for the introduction of digital broadcasting in European, African, Arab, CIS countries and Iran. The preparation of the plan involved complex calculations under short deadline and required dependable and efficient computing capability. The ITU designed and deployed in-situ a dedicated PC farm, in parallel to the European Organization for Nuclear Research (CERN) which provided and supported a system based on the EGEE Grid. The planning cycle at the RRC06 required a periodic execution in the order of 200,000 short jobs, using several hundreds of CPU hours, in a period of less than 12 hours. The nature of the problem required dynamic workload-balancing and low-latency access to the computing resources. We present the strategy and key technical choices that delivered a reliable service to the RRC06

HPV vaccination of immunocompromised hosts.

It is well-established that immunocompromised people are at increased risk of HPV-related disease compared with those who are immunocompetent. Prophylactic HPV sub-unit vaccines are safe and immunogenic in immunocompromised people and it is strongly recommended that vaccination occur according to national guidelines. When delivered to immunocompromised populations, HPV vaccines should be given as a 3-dose regimen

Anaphe - OO Libraries and Tools for Data Analysis

The Anaphe project is an ongoing effort to provide an Object Oriented software environment for data analysis in HENP experiments. A range of commercial and public domain libraries is used to cover basic functionalities; on top of these libraries a set of HENP-specific C++ class libraries for histogram management, fitting, plotting and ntuple-like data analysis has been developed. In order to comply with the user requirements for a command-line driven tool, we have chosen to use a scripting language (Python) as the front-end for a data analysis tool. The loose coupling provided by the consequent use of (AIDA compliant) Abstract Interfaces for each component in combination with the use of shared libraries for their implementation provides an easy integration of existing libraries into modern scripting languages thus allowing for rapid application development. This integration is simplified even further using a specialised toolkit (SWIG) to create "shadow classes" for the Python language, which map the definitions of the Abstract Interfaces almost at a one-to-one level. This paper will give an overview of the architecture and design choices and will present the current status and future developments of the project

Weight Loss and Illness Severity in Adolescents With Atypical Anorexia Nervosa.

BACKGROUND:Lower weight has historically been equated with more severe illness in anorexia nervosa (AN). Reliance on admission weight to guide clinical concern is challenged by the rise in patients with atypical anorexia nervosa (AAN) requiring hospitalization at normal weight. METHODS:We examined weight history and illness severity in 12- to 24-year-olds with AN (n = 66) and AAN (n = 50) in a randomized clinical trial, the Study of Refeeding to Optimize Inpatient Gains (www.clinicaltrials.gov; NCT02488109). Amount of weight loss was the difference between the highest historical percentage median BMI and admission; rate was the amount divided by duration (months). Unpaired t tests compared AAN and AN; multiple variable regressions examined associations between weight history variables and markers of illness severity at admission. Stepwise regression examined the explanatory value of weight and menstrual history on selected markers. RESULTS:Participants were 16.5 ± 2.6 years old, and 91% were of female sex. Groups did not differ by weight history or admission heart rate (HR). Eating Disorder Examination Questionnaire global scores were higher in AAN (mean 3.80 [SD 1.66] vs mean 3.00 [SD 1.66]; P = .02). Independent of admission weight, lower HR (β = -0.492 [confidence interval (CI) -0.883 to -0.100]; P = .01) was associated with faster loss; lower serum phosphorus was associated with a greater amount (β = -0.005 [CI -0.010 to 0.000]; P = .04) and longer duration (β = -0.011 [CI -0.017 to 0.005]; P = .001). Weight and menstrual history explained 28% of the variance in HR and 36% of the variance in serum phosphorus. CONCLUSIONS:Weight history was independently associated with markers of malnutrition in inpatients with restrictive eating disorders across a range of body weights and should be considered when assessing illness severity on hospital admission

ENGINEERING STUDIES OF AN EXPERIMENTAL OPEN OCEAN SEAWEED FARM DESIGN

An experimental seaweed cultivation system was designed for offshore and exposed locations. Novel features were incorporated to address major barriers to the expansion of seaweed, including limited nearshore and sheltered marine locations and risk of marine animal entanglement. A novel modular mooring geometry was developed to improve stability, reliability, and spatial efficiency to achieve an economical and robust structure viable in high wave energy environments. Stiff composite (e.g. fiberglass) rods were used to replace components typically made of rope, including mooring lines and seaweed growth substrate (cultivation lines), as a means to mitigate risk of marine animal entanglement. This dissertation presents a collection of studies testing and evaluating the farm structural design, its component technologies, and the associated engineering tools and methods. A demonstration farm applying the experimental design concept was designed, deployed, planted with sugar kelp, instrumented, monitored, harvested and recovered at a shallow but exposed location in the Gulf of Maine. Observations indicated structural robustness, with survival and successful kelp growth in several severe winter storms, one with wave heights up to 5.9 m. Noted areas for improvement included kelp attachment strength to the fiberglass growth substrate, and mooring tension management for farm operations and reduced kelp loss. Instruments for measuring local waves, currents and mooring tensions were deployed on and near the demonstration farm. Response amplitude operators and linear regression models of tension response statistics were calculated from datasets of weeks-long in-situ measurements, providing insight into the behavior of the demonstration farm and its novel mooring geometry. The system was found to exhibit a strong sensitivity to tide level, a desirably mild response to waves with periods of 6 to 15 seconds, a problematic response to high frequency waves with period of 1 to 6 seconds, a relatively low magnitude low frequency response associated with wave group envelopes and a tendency for damaging shock loading when mooring stiffness was elevated. The demonstration kelp farm was numerically simulated in ocean conditions measured at the deployment site using two models: one defined by design phase assumptions, and another informed by detailed measurements of the deployed system. Engineering relevant results were compared amongst the two model variants, and to in-situ measurements. Results confirmed that when model inputs more accurately reflected reality, especially mooring pretension and kelp biomass, the replicability of in situ measurements improved substantially. For at least one mooring component, differences between simulation results and in situ measurements, in the context of significant tension magnitudes, were within margins typically accepted for aquaculture mooring engineering purposes. Two versions of a commercial scale kelp farm using the novel mooring geometry were evaluated for their structural behavior and costs: one used conventional nylon ropes throughout (except anchor chain) and the other used fiberglass rods as mooring lines and cultivation lines. Numerical simulations of the two farm variants in design-relevant wave and current conditions (for the Gulf of Maine) indicated substantially higher loads on the composite rod based farm, however, economic analyses using these results indicated only a marginal differences in required structural capital investment. Using the demonstration farm as a case study, anchoring for exposed seaweed farms was considered from the perspective of anchor type selection, site characterization, anchor design, installation approaches, and risk mitigation methods. During decommissioning of the demonstration farm several of the anchors used, including a novel multi-line / multi-shaft helical anchor, were pulled to failure under measured tensile loads. These measurements were compared to original engineering design targets, revealing that assumptions informed by geophysical surveys and sediment sampling contributed to conservative estimates for anchor holding capacity

Altered Toll-like receptor expression and function in HPV-associated oropharyngeal carcinoma

Toll-like receptors (TLRs) have been widely investigated due to their importance in the inflammatory response and possible links to tumor promotion/regression and prognosis. In cancers with an infective etiology, such as human papillomavirus (HPV)-associated Oropharyngeal Squamous Cell Carcinoma (OPSCC), TLR responses may be activated and play a role in tumorigenesis. Our aim was to assess the expression of all TLRs in OPSCC cell lines (both HPV+and HPV-) by qPCR, Western Blot and flow cytometry and assess their response to TLR ligands lipopolysaccharide (LPS), LPS ultra-pure (LPS-UP) and peptidoglycan (PGN) by analyzing IL-8 and IL-6 production. We also immunostained 61 OPSCC tissue samples with anti-TLR4. Results showed lower TLR1 and TLR6 mRNA expression and higher TLR9 protein expression in HPV+when compared to HPV-OPSCC cells. TLR4 expression did not vary by HPV status in OPSCC cells, but TLR4 expression was significantly lower in HPV+OPSCC tissues. After stimulation with PGN, only one cell line (HPV+) did not secrete IL-6 or IL-8. Furthermore, HPV+OPSCC lines showed no IL-6 or IL-8 production on treatment with LPS/LPS-UP. The data suggest changes in TLR4 signaling in HPV+OPSCC, since we have shown lower tissue expression of TLR4 and no pro-inflammatory response after stimulation with LPS and LPS-UP. Also, it suggests that OPSCC may respond to HPV infection by increased expression of TLR9. This study demonstrates differences in expression and function of TLRs in OPSCC, which are dependent on HPV status, and may indicate subversion of the innate immune response by HPV infection