Development of self-repair nano-rod scaffold materials for implantation of osteosarcoma affected bone tissue

Osteosarcoma is the most widely recognized fatal bone disease in children and young adults. The osteosarcoma affected places of bone implant materials lose their activity after a period of time due to the possibility of regenerating sarcoma cells. Hence, the complete recovery of this disease is very challenging. Subsequently, new helpful methodologies, including natural antioxidant loaded bone implant materials, are effectively used to treat osteosarcoma cells. In this regard, nano-hydroxyapatite reinforced with a xylitol based poly(xylitol sebacate) PXS co-polymer together with a capsaicin loaded scaffold was investigated on osteosarcoma cells. The physicochemical properties of the scaffold were evaluated by FT-IR (Fourier transform infrared spectroscopy), SEM (scanning electron microscopy), TEM (transmission electron microscopy), and XRD (X-ray diffraction). The in vitro release and antioxidant activity of the capsaicin loaded nHAP/PXS/CAP scaffold were evaluated by UV-Visible spectroscopy. Cytotoxicity against the Saos-2 cancer line and cell viability in the osteoblast cell MG63 are reported. Eventually, the composite enlarges the creation of reactive oxygen species (ROS) in Saos-2 cells

A phosphorylated chitosan armed hydroxyapatite nanocomposite for advancing activity on<i>osteoblast</i>and<i>osteosarcoma</i>cells

Recently, applications of traditional medicine in tissue engineering have gained increasing attention.</p

Pulsed Electrodeposition of HAP/CPG-BSA/CUR Nanocomposite on Titanium Metal for Potential Bone Regeneration

Bioactive hydroxyapatite (HAP) composites are progressively predicted as successive materials in bone regeneration therapy. A nanostructured composite made from the mixture of hydroxyapatite, chitosan (C)-polyethylene glycol (P)-gelatin (G), and bovine serum albumin (BSA) (HAP/CPG- BSA) was prepared using ultra probe sonication followed by lyophilization. The anti-inflammatory and antioxidant-rich curcumin (CUR) molecules were impregnated on HAP/CPG-BSA composite for self-repairing bone regeneration. The physicochemical morphology of HAP/CPG-BSA/CUR composite was analyzed by FTIR, XRD, SEM, and TEM techniques. The 92.0% of CUR release was observed from HAP/CPG-BSA/CUR composite by UV–VIS spectroscopy at a λmax value of 420 nm. The in vitro studies of HAP/CPG-BSA/CUR against osteoblast-like (MG63) cells and fibroblast cells (L929) resulted from 90.0 and 91.0% of cell viability for significant improvement in osteoinduction, proliferation, and viability for bone regeneration. The in vivo results of bone-cell formation by the implantation suggest that HAP/CPG-BSA/CUR composite might be useful in applications of regeneration medicine. The outcome of this study shows the ability to favor bone regeneration and signifies that this may be a prospective substitute implant for elevated repairing of bone

Cisplatin-Loaded Graphene Oxide/Chitosan/Hydroxyapatite Composite as a Promising Tool for Osteosarcoma-Affected Bone Regeneration

Presently, tissue engineering approaches have been focused toward finding new potential scaffolds with osteoconductivity on bone-disease-affected cells. This work focused on the cisplatin (CDDP)-loaded graphene oxide (GO)/hydroxyapatite (HAP)/chitosan (CS) composite for enhancing the growth of osteoblast cells and prevent the development of osteosarcoma cells. The prepared composites were characterized for the confirmation of composite formation using Fourier transform infrared spectroscopy, scanning electron microscopy, transmission electron microscopy, and X-ray diffraction techniques. A flower like morphology was observed for the GO/HAP/CS-3/CDDP composite. UV−vis spectroscopy was used to observe the controlled release of CDDP from the GO/HAP/CS-3/CDDP composite, and 67.34% of CDDP was released from the composite over a time period of 10 days. The GO/HAP/CS-3/CDDP nanocomposites showed higher viability in comparison with GO/HAP/CS-3 on MG63 osteoblast-like cells and higher cytotoxicity against cancer cells (A549). The synthesized composite was found to show enhanced proliferative, adhesive, and osteoinductive effects on the alkaline phosphatase activity of osteoblast-like cells. Our results suggested that the CDDP-loaded GO/HAP/CS-3 nanocomposite has an immense prospective as a bone tissue replacement in the bone-cancer-affected tissues