Effects of infestation by cowpea aphid (Homoptera: Aphididae) on different growth stages of resistant and susceptible cowpea cultivars

Field studies were conducted to characterise the effects of infestations by adult and nymphal stages of cowpea aphid, Aphis craccivora Koch, on the growth and yield of cowpea, Vigna unguiculata (L.) Walp. Seedling, flowering, and podding stage plants of aphid-resistant (cv. ICV-12) and aphid-susceptible (cv. ICV-1) cowpea cultivars were used in the studies. Four treatments (consisting of infestations with adult and nymphal aphids, caged controls and uncaged controls) were administered on plants for 22 days post-treatment. Eight parameters of crop success were measured: extended leaf heights (ELH); plant mortality; incidence of sooty mould; incidence and abundance of natural enemy species; crop growth parameters (net assimilation rate, [NAR] in g/ dm2/day, and crop growth rate [CGR] in g/dm2 land surface/day); and plant yields (seeds per pod, weight per seed). Data were analysed using analysis of variance (ANOVA), orthogonal contrasts and 95% confidence intervals (C.I.). There were no significant (P > 0.05) differences between adult and nymphal infestations or between caged and uncaged controls, so the respective sets of data were combined for comparisons of aphid infestations with control treatments. Infestations caused severe plant stunting and other growth deformities, drastic yield reductions, higher plant mortality, greater incidence of natural enemies and abundance of Cheilomenes spp. on cv. ICV-1 than on cv. ICV-12, and on infested and uninfested plants. Aphid infestations did not significantly affect the incidence of sooty mould on plants of cv. ICV-12 or cv. ICV-

Pattern and rate of within-field dispersal and bionomics of the cowpea aphid, Aphis craccivora (Aphididae), on selected cowpea cultivars

Comparative field studies were conducted during the rainy and dry seasons at the Mbita Point Field Station of ICIPE in southwestern Kenya, to investigate the pattern and rate of dispersal of Aphis craccivora on aphid-resistant (ICV-12) and aphid-susceptible (ICV-1) cowpea cultivars in relation to key weather factors. The effects of the dispersal trends on crop performance and aphid population dynamics were analysed. Treatments consisted of initial aphid releases at the north, south, west, east and centre of test plots and uninfested controls maintained on plants for 22 days. Parameters recorded included: pattern (direction of spread of aphids) and rate (number of aphid-infested plants at a given time) in test plots; crop growth and yields and associated factors such as incidence of sooty mould and plant mortality, and aphid density and associated factors, including the incidence of natural enemy species, particularly coccinellids. Dispersal was fastest when releases were made in the west, north and centre of plots, and resulted in adveVse effects on ICV-1 growth and yields. Infestations of ICV-12 did not significantly affect crop performance. There was an apparent direct, positive relationship between wind direction and pattern of spread of aphid infestations, but this pattern was more apparent during the rainy season, when wind speeds were higher, than during the dry season. The incidence of natural enemy species correlated with the spread of aphid infestations, while the abundance of coccinellids correlated with aphid densit

Observable implications of geometrical and dynamical aspects of freeze-out in heavy ion collisions

Using an analytical parameterization of hadronic freeze-out in relativistic heavy ion collisions, we present a detailed study of the connections between features of the freeze-out configuration and physical observables. We focus especially on anisotropic freeze-out configurations (expected in general for collisions at finite impact parameter), azimuthally-sensitive HBT interferometry, and final-state interactions between non-identical particles. Model calculations are compared with data taken in the first year of running at RHIC; while not perfect, good agreement is found, raising the hope that a consistent understanding of the full freeze-out scenario at RHIC is possible, an important first step towards understanding the physics of the system prior to freeze-out.Comment: 36 pages, 56 figures, 2 tables; version accepted for publication in PRC: some figures, references and discussion added; now also discusses classical versus quantum statistic

Stylet Penetration Activities by Aphis craccivora (Homoptera: Aphididae) on Plants and Excised Plant Parts of Resistant and Susceptible Cultivars of Cowpea (Leguminosae)

Direct current electrical penetration graphs (DC-EPGs) were used to analyze the stylet penetration activities of cowpea aphid, Aphis craccivora Koch, on plants of aphid-resistant (ICV-12) and aphid-susceptible (ICV-1) cultivars of cowpea, Vigna unguiculata (L.) Walpers. Aphid stylet penetration on whole plants at seedling, flowering, and podding stages were studied in one experiment, and in another experiment excised leaves from seedling plants, excised flowers, and excised pods were tested. Electrical signals depicting the aphid stylet penetration activities on their host plants were amplified, recorded onto a paper chart recorder, and scored for specific waveform patterns. Compared with similar tissues of ICV-1, intact leaves and excised seedling foliage of ICV-12 plants caused severe disruption of aphid stylet penetration activities. This was manifested in frequent penetration attempts that were abruptly terminated or unsustained, and in shorter penetration times, signifying antixenosis resistance in ICV-12. There was reduced occurrence of E waveforms, which represent stylet activity in plant vascular tissues. Also, prior exposure of test aphids to plants of one cultivar did not significantly influence the expected stylet penetration activities on plants of the other cultivar. Overall, ICV-12 exhibited high levels of resistance against A. craccivor

Linking Community Participatory Research to Global Policymaking: Lessons Learned

The past two decades saw a proliferation of opportunities for the perspectives of people experiencing poverty and marginalisation to input into global policymaking spaces. So far these efforts have been contested, with attempts to embed participatory methodologies facilitating only limited consultation and falling into many pitfalls. While Participate was built on learning from previous attempts to influence global policy, we aimed to further understand participatory processes, and advance practical mechanisms for participation at every level of decision-making, from local to national and global. Participate emphasises the interactions between these levels and the importance of impacting multiple levels in order to create lasting, transformative change. This chapter describes what has been learnt about bringing the voices on the ground into global policymaking. We examine how and by whom the knowledge and evidence created were used, and the barriers and challenges to embedding this knowledge into global policy processes, through the following questions: • What have been the challenges to embedding participatory processes into global policy spaces? • What have these pathways of influence looked like, and what are some of the key lessons learned from these

Truly reconciled? A dyadic analysis of post-conflict social reintegration in Northern Uganda

In the aftermath of civil war or violent internal conflict, one of the key peacebuilding challenges is the reconciliation of former enemies who are members of the same small-scale societies. A failure of social reintegration may contribute to what is known as a conflict trap. To detect lingering hostile attitudes among a community’s various factions is crucial, but the approaches adopted in previous studies tend to focus on the impact of conflict on one or other aggregated indicator of social cohesion rather than on how violence-affected individuals regard and act towards their fellow community members. Here we demonstrate the value of concentrating on this latter dyadic component of social interactions and we use behavioural experiments and a social tie survey to assess, in an appropriately disaggregated manner, social cohesion in a post-conflict setting in northern Uganda. Whereas in self-reported surveys, ex-combatants appear to be well-connected, active members of their communities, the experiments unveil the continued reluctance of other community members to share or cooperate with them; fewer resources are committed to ex-combatants than to others, which is statistically significant. The dyadic nature of our analysis allows us to detect which groups are more prone to discriminate against ex-combatants, which may help facilitate targeted interventions

A novel method to improve temperature simulations of general circulation models based on ensemble empirical mode decomposition and its application to multi-model ensembles

A novel method based on the ensemble empirical mode decomposition (EEMD) method was developed to improve model performance. This method was evaluated by applying it to global surface air temperatures, which were simulated by eight general circulation models from the Coupled Model Intercomparison Project Phase 5 (CMIP5). The temperature simulations of the eight models were separated into their different components by EEMD. The model's performance improved after the first high-frequency component was removed from the original simulations by EEMD for each model, on both the global and continental scale. Moreover, EEMD was more effective in improving the model's performance compared to the wavelet transform method. The multi-model ensembles (MMEs) were calculated based on the EEMD-improved model simulations using the Average Ensemble Mean, Multiple Linear Regression, Singular Value Decomposition and Bayesian Model Averaging methods. The results showed that the MME forecasts performed better when the calculations were based on the EEMD-improved simulations as opposed to the original simulations on both the global and continental scale. Therefore, the results of the MME were further improved by using the EEMD-improved model simulations. This new method provides a simple way to improve model performance and can be easily applied to further improve MME simulations

Back to the Future: The Uses of Television in the Digital Age

This article considers some of the present-day issues, challenges and possibilities facing television broadcasting via a critical examination of the recently published Goldsmiths report on the future of public service television in the twenty-first century. Focusing mainly on UK terrestrial broadcasters (BBC, ITV, Channel 4 and Channel 5), the article summarises and expands on the report's key findings and recommendations, particularly in relation to questions concerning digitalisation, content, diversity, quality, marketisation, funding and national and regional heritage. The article argues that, despite the rise of the Internet and the proliferation of digital platforms, television viewing remains a common source of information and entertainment and is characterised by meaningful continuities. Additionally, the article outlines the vitally important role played by David Puttnam, chair of the Goldsmiths inquiry, in defending public service television through his active engagement with relevant parliamentary committees and as a widely respected media professional. Finally, the article reflects on the continuing relevance of the 1962 Pilkington Report on Broadcasting, which was similarly commissioned in order to evaluate the purposes of television. In so doing, the article suggests that Pilkington's criticisms of creeping commercialism and the ensuing regulatory proposals still represent a cogent engagement with the idea of public service broadcasting as a primary facilitator of deliberative democracy

In vitro anti-malarial interaction and gametocytocidal activity of cryptolepine

YesBackground: Discovery of novel gametocytocidal molecules is a major pharmacological strategy in the elimination and eradication of malaria. The high patronage of the aqueous root extract of the popular West African anti-malarial plant Cryptolepis sanguinolenta (Periplocaceae) in traditional and hospital settings in Ghana has directed this study investigating the gametocytocidal activity of the plant and its major alkaloid, cryptolepine. This study also investigates the anti-malarial interaction of cryptolepine with standard anti-malarials, as the search for new anti-malarial combinations continues. Methods: The resazurin-based assay was employed in evaluating the gametocytocidal properties of C. sanguinolenta and cryptolepine against the late stage (IV/V) gametocytes of Plasmodium falciparum (NF54). A fixed ratio method based on the SYBR Green I fluorescence-based assay was used to build isobolograms from a combination of cryptolepine with four standard anti-malarial drugs in vitro using the chloroquine sensitive strain 3D7. Results: Cryptolepis sanguinolenta ( IC50 = 49.65 nM) and its major alkaloid, cryptolepine ( IC50 = 1965 nM), showed high inhibitory activity against the late stage gametocytes of P. falciparum (NF54). In the interaction assays in asexual stage, cryptolepine showed an additive effect with both lumefantrine and chloroquine with mean ΣFIC50s of 1.017 ± 0.06 and 1.465 ± 0.17, respectively. Cryptolepine combination with amodiaquine at therapeutically relevant concentration ratios showed a synergistic effect (mean ΣFIC50 = 0.287 ± 0.10) whereas an antagonistic activity (mean ΣFIC50 = 4.182 ± 0.99) was seen with mefloquine. Conclusions: The findings of this study shed light on the high gametocytocidal properties of C. sanguinolenta and cryptolepine attributing their potent anti-malarial activity mainly to their effect on both the sexual and asexual stages of the parasite. Amodiaquine is a potential drug partner for cryptolepine in the development of novel fixed dose combinations

Blood Bank Practices for Sickle Cell Patients in North Carolina

Sickle cell disease (SCD) remains an important public health problem. Predominantly affecting African Americans, SCD is associated with significant health, financial, and psychosocial costs. Therefore, it is vital to find new ways to improve delivery of care to this patient population. The hospital blood bank plays a key role in delivering transfusion therapy to SCD patients. Because transfusion therapy represents the mainstay of treatment for most SCD patients, we hypothesized that a systematic review of blood bank practices for these patients might uncover new opportunities to improve this care. Current blood bank practices for SCD patient have not been previously described. This paper discusses blood bank practices for SCD patients in North Carolina. The layout is as follows. First, the underlying cause, history, and costs of sickle cell disease are described. Next, evidence supporting the use of transfusions for SCD management and its associated risks are considered. Data from a crosssectional study of NC blood bank practices for SCD patients are then presented. Finally, the significance and implications of this research are discussed.Master of Public Healt