Observation of O+ 4P-4D0 lines in proton aurora over Svalbard

Spectra of a proton aurora event show lines of O+ 4P-4D0 multiplet (4639–4696 Å) enhanced relative to the N2 +1N(0,2) compared to normal electron aurora. Conjugate satellite particle measurements are used as input to electron and proton transport models, to show that p/H precipitation is the dominant source of both the O+ and N2 +1N emissions. The emission cross-section of the multiplet in p collisions with O and O2 estimated from published work does not explain the observed O+ brightness, suggesting a higher emission cross-section for low energy p impact on O

Ocean currents shape the microbiome of Arctic marine sediments

Prokaryote communities were investigated on the seasonally stratified Alaska Beaufort Shelf (ABS). Water and sediment directly underlying water with origin in the Arctic, Pacific or Atlantic oceans were analyzed by pyrosequencing and length heterogeneity-PCR in conjunction with physicochemical and geographic distance data to determine what features structure ABS microbiomes. Distinct bacterial communities were evident in all water masses. Alphaproteobacteria explained similarity in Arctic surface water and Pacific derived water. Deltaproteobacteria were abundant in Atlantic origin water and drove similarity among samples. Most archaeal sequences in water were related to unclassified marine Euryarchaeota. Sediment communities influenced by Pacific and Atlantic water were distinct from each other and pelagic communities. Firmicutes and Chloroflexi were abundant in sediment, although their distribution varied in Atlantic and Pacific influenced sites. Thermoprotei dominated archaea in Pacific influenced sediments and Methanomicrobia dominated in methane-containing Atlantic influenced sediments. Length heterogeneity-PCR data from this study were analyzed with data from methane-containing sediments in other regions. Pacific influenced ABS sediments clustered with Pacific sites from New Zealand and Chilean coastal margins. Atlantic influenced ABS sediments formed another distinct cluster. Density and salinity were significant structuring features on pelagic communities. Porosity co-varied with benthic community structure across sites and methane did not. This study indicates that the origin of water overlying sediments shapes benthic communities locally and globally and that hydrography exerts greater influence on microbial community structure than the availability of methane

Root exudate analogues accelerate CO 2 and CH 4 production in tropical peat

Root exudates represent a large and labile carbon input in tropical peatlands, but their contribution to carbon dioxide (CO2) and methane (CH4) production remains poorly understood. Changes in species composition and productivity of peatland plant communities in response to global change could alter both inputs of exudates and associated greenhouse gas emissions. We used manipulative laboratory incubations to assess the extent to which root exudate quantity and chemical composition drives greenhouse gas emissions from tropical peatlands. Peat was sampled from beneath canopy palms (Raphia taedigera) and broadleaved evergreen trees (Campnosperma panamensis) in an ombrotrophic wetland in Panama. Root exudate analogues comprising a mixture of sugars and organic acids were added in solution to peats derived from both species, with CO2 and CH4 measured over time. CO2 and CH4 production increased under most treatments, but the magnitude and duration of the response depended on the composition of the added labile carbon mixture rather than the quantity of carbon added or the botanical origin of the peat. Treatments containing organic acids increased soil pH and altered other soil properties including redox potential but did not affect the activities of extracellular hydrolytic enzymes. CO2 but not CH4 production was found to be linearly related to microbial activity and redox potential. Our findings demonstrate the importance of root exudate composition in regulating greenhouse gas fluxes and propose that in situ plant species changes, particularly those associated with land use change, may account for small scale spatial variation in CO2 and CH4 fluxes due to species specific root exudate compositions

The biogeography of abundant and rare bacterioplankton in the lakes and reservoirs of China.

Bacteria play key roles in the ecology of both aquatic and terrestrial ecosystems; however, little is known about their diversity and biogeography, especially in the rare microbial biosphere of inland freshwater ecosystems. Here we investigated aspects of the community ecology and geographical distribution of abundant and rare bacterioplankton using high-throughput sequencing and examined the relative influence of local environmental variables and regional (spatial) factors on their geographical distribution patterns in 42 lakes and reservoirs across China. Our results showed that the geographical patterns of abundant and rare bacterial subcommunities were generally similar, and both of them showed a significant distance-decay relationship. This suggests that the rare bacterial biosphere is not a random assembly, as some authors have assumed, and that its distribution is most likely subject to the same ecological processes that control abundant taxa. However, we identified some differences between the abundant and rare groups as both groups of bacteria showed a significant positive relationship between sites occupancy and abundance, but the abundant bacteria exhibited a weaker distance-decay relationship than the rare bacteria. Our results implied that rare subcommunities were mostly governed by local environmental variables, whereas the abundant subcommunities were mainly affected by regional factors. In addition, both local and regional variables that were significantly related to the spatial variation of abundant bacterial community composition were different to those of rare ones, suggesting that abundant and rare bacteria may have discrepant ecological niches and may play different roles in natural ecosystems

An advanced light scattering apparatus for investigating soft matter onboard the International Space Station

Colloidal solids (COLIS) is a state-of-the-art light scattering setup developed for experiments onboard the International Space Station (ISS). COLIS allows for probing the structure and dynamics of soft matter systems on a wide range of length scales, from a few nm to tens of microns, and on time scales from 100 ns to tens of hours. In addition to conventional static and dynamic light scattering, COLIS includes depolarized dynamic light scattering, a small-angle camera, photon correlation imaging, and optical manipulation of thermosensitive samples through an auxiliary near-infrared laser beam, thereby providing a unique platform for probing soft matter systems. We demonstrate COLIS through ground tests on standard Brownian suspensions, and on protein, colloidal glasses, and gel systems similar to those to be used in future ISS experiments

Tara Pacific Expedition\u27s atmospheric measurements of marine aerosols across the Atlantic and Pacific Oceans: Overview and preliminary results

Marine aerosols play a significant role in the global radiative budget, in clouds\u27 processes, and in the chemistry of the marine atmosphere. There is a critical need to better understand their production mechanisms, composition, chemical properties, and the contribution of ocean-derived biogenic matter to their mass and number concentration. Here we present an overview of a new dataset of in situ measurements of marine aerosols conducted over the 2.5-yr Tara Pacific Expedition over 110, 000 km across the Atlantic and Pacific Oceans. Preliminary results are presented here to describe the new dataset that will be built using this novel set of measurements. It will characterize marine aerosols properties in detail and will open a new window to study the marine aerosol link to the water properties and environmental conditions

Far-ultraviolet aurora identified at comet 67P/ Churyumov-Gerasimenko

Having a nucleus darker than charcoal, comets are usually detected from Earth through the emissions from their coma. The coma is an envelope of gas that forms through the sublimation of ices from the nucleus as the comet gets closer to the Sun. In the far-ultraviolet portion of the spectrum, observations of comae have revealed the presence of atomic hydrogen and oxygen emissions. When observed over large spatial scales as seen from Earth, such emissions are dominated by resonance fluorescence pumped by solar radiation. Here, we analyse atomic emissions acquired close to the cometary nucleus by the Rosetta spacecraft and reveal their auroral nature. To identify their origin, we undertake a quantitative multi-instrument analysis of these emissions by combining coincident neutral gas, electron and far-ultraviolet observations. We establish that the atomic emissions detected from Rosetta around comet 67P/Churyumov-Gerasimenko at large heliocentric distances result from the dissociative excitation of cometary molecules by accelerated solar-wind electrons (and not by electrons produced from photo-ionization of cometary molecules). Like the discrete aurorae at Earth and Mars, this cometary aurora is driven by the interaction of the solar wind with the local environment. We also highlight how the oxygen line O I at wavelength 1,356 Å could be used as a tracer of solar-wind electron variability

Saturn's atmospheric response to the large influx of ring material inferred from Cassini INMS measurements

During the Grand Finale stage of the Cassini mission, organic-rich ring material was discovered to be flowing into Saturn's equatorial upper atmosphere at a surprisingly large rate. Through a series of photochemical models, we have examined the consequences of this ring material on the chemistry of Saturn's neutral and ionized atmosphere. We find that if a substantial fraction of this material enters the atmosphere as vapor or becomes vaporized as the solid ring particles ablate upon atmospheric entry, then the ring-derived vapor would strongly affect the composition of Saturn's ionosphere and neutral stratosphere. Our surveys of Cassini infrared and ultraviolet remote-sensing data from the final few years of the mission, however, reveal none of these predicted chemical consequences. We therefore conclude that either (1) the inferred ring influx represents an anomalous, transient situation that was triggered by some recent dynamical event in the ring system that occurred a few months to a few tens of years before the 2017 end of the Cassini mission, or (2) a large fraction of the incoming material must have been entering the atmosphere as small dust particles less than ~100 nm in radius, rather than as vapor or as large particles that are likely to ablate. Future observations or upper limits for stratospheric neutral species such as HC$_3$N, HCN, and CO$_2$ at infrared wavelengths could shed light on the origin, timing, magnitude, and nature of a possible vapor-rich ring-inflow event.Comment: accepted in Icaru

Two-Stage Clustering (TSC): A Pipeline for Selecting Operational Taxonomic Units for the High-Throughput Sequencing of PCR Amplicons

Clustering 16S/18S rRNA amplicon sequences into operational taxonomic units (OTUs) is a critical step for the bioinformatic analysis of microbial diversity. Here, we report a pipeline for selecting OTUs with a relatively low computational demand and a high degree of accuracy. This pipeline is referred to as two-stage clustering (TSC) because it divides tags into two groups according to their abundance and clusters them sequentially. The more abundant group is clustered using a hierarchical algorithm similar to that in ESPRIT, which has a high degree of accuracy but is computationally costly for large datasets. The rarer group, which includes the majority of tags, is then heuristically clustered to improve efficiency. To further improve the computational efficiency and accuracy, two preclustering steps are implemented. To maintain clustering accuracy, all tags are grouped into an OTU depending on their pairwise Needleman-Wunsch distance. This method not only improved the computational efficiency but also mitigated the spurious OTU estimation from ‘noise’ sequences. In addition, OTUs clustered using TSC showed comparable or improved performance in beta-diversity comparisons compared to existing OTU selection methods. This study suggests that the distribution of sequencing datasets is a useful property for improving the computational efficiency and increasing the clustering accuracy of the high-throughput sequencing of PCR amplicons. The software and user guide are freely available at http://hwzhoulab.smu.edu.cn/paperdata/