Direct and long-lasting effects elicited by repeated drug administration on 50-kHz ultrasonic vocalizations are regulated differently: Implications for the study of the affective properties of drugs of abuse

Several studies suggest that 50-kHz ultrasonic vocalizations (USVs) may indicate a positive affective state in rats, and these vocalizations are increasingly being used to investigate the properties of psychoactive drugs. Previous studies, however, have focused on dopaminergic psychostimulants and morphine, whereas little is known about how other drugs modulate 50-kHz USVs. To further elucidate the neuropharmacology of 50-kHz USVs, the present study characterized the direct and long-lasting effects of different drugs of abuse, by measuring the number of 50-kHz USVs and their 'trill' subtype emitted by adult male rats. Rats received repeated administrations of amphetamine (2 mg/kg, i.p.), 3,4-methylenedioxymethamphetamine (MDMA, 7.5 mg/kg, i.p.), morphine (7.5 mg/kg, s.c.), or nicotine (0.4 mg/kg, s.c.), on either consecutive or alternate days (five administrations in total) in a novel environment. Seven days later, rats were re-exposed to the drug-paired environment, subjected to USVs recording, and then challenged with the same drug. Finally, 7 d after the challenge, rats were repeatedly exposed to the drug-paired environment and vocalizations were measured. Amphetamine was the only drug to stimulate 50-kHz USVs and 'trill' subtype emission during administration and challenge. Conversely, all rats emitted 50-kHz USVs when re-exposed to the test cage, and this effect was most marked in morphine-treated rats, and less evident in nicotine-treated rats. This study demonstrates that the direct and long-lasting effects of drugs on 50-kHz USVs are regulated differently, providing a better understanding of the usefulness of these vocalizations in the study of psychoactive drugs

Associative memory on a small-world neural network

We study a model of associative memory based on a neural network with small-world structure. The efficacy of the network to retrieve one of the stored patterns exhibits a phase transition at a finite value of the disorder. The more ordered networks are unable to recover the patterns, and are always attracted to mixture states. Besides, for a range of the number of stored patterns, the efficacy has a maximum at an intermediate value of the disorder. We also give a statistical characterization of the attractors for all values of the disorder of the network.Comment: 5 pages, 4 figures (eps

Evolution of foot-and-mouth disease virus intra-sample sequence diversity during serial transmission in bovine hosts

RNA virus populations within samples are highly heterogeneous, containing a large number of minority sequence variants which can potentially be transmitted to other susceptible hosts. Consequently, consensus genome sequences provide an incomplete picture of the within- and between-host viral evolutionary dynamics during transmission. Foot-and-mouth disease virus (FMDV) is an RNA virus that can spread from primary sites of replication, via the systemic circulation, to found distinct sites of local infection at epithelial surfaces. Viral evolution in these different tissues occurs independently, each of them potentially providing a source of virus to seed subsequent transmission events. This study employed the Illumina Genome Analyzer platform to sequence 18 FMDV samples collected from a chain of sequentially infected cattle. These data generated snap-shots of the evolving viral population structures within different animals and tissues. Analyses of the mutation spectra revealed polymorphisms at frequencies >0.5% at between 21 and 146 sites across the genome for these samples, while 13 sites acquired mutations in excess of consensus frequency (50%). Analysis of polymorphism frequency revealed that a number of minority variants were transmitted during host-to-host infection events, while the size of the intra-host founder populations appeared to be smaller. These data indicate that viral population complexity is influenced by small intra-host bottlenecks and relatively large inter-host bottlenecks. The dynamics of minority variants are consistent with the actions of genetic drift rather than strong selection. These results provide novel insights into the evolution of FMDV that can be applied to reconstruct both intra- and inter-host transmission routes

Self-propelled particles with fluctuating speed and direction of motion

We study general aspects of active motion with fluctuations in the speed and the direction of motion in two dimensions. We consider the case in which fluctuations in the speed are not correlated to fluctuations in the direction of motion, and assume that both processes can be described by independent characteristic time-scales. We show the occurrence of a complex transient that can exhibit a series of alternating regimes of motion, for two different angular dynamics which correspond to persistent and directed random walks. We also show additive corrections to the diffusion coefficient. The characteristic time-scales are also exposed in the velocity autocorrelation, which is a sum of exponential forms.Comment: to appear in Phys. Rev. Let

Involvement of Glutamate NMDA Receptors in the Acute, Long-Term, and Conditioned Effects of Amphetamine on Rat 50kHz Ultrasonic Vocalizations

BACKGROUND: Rats emit 50kHz ultrasonic vocalizations (USVs) in response to either natural or pharmacological pleasurable stimuli, and these USVs have emerged as a new behavioral measure for investigating the motivational properties of drugs. Earlier studies have indicated that activation of the dopaminergic system is critically involved in 50kHz USV emissions. However, evidence also exists that non-dopaminergic neurotransmitters participate in this behavioral response. METHODS:To ascertain whether glutamate transmission plays a role in 50kHz USV emissions stimulated by amphetamine, rats received five amphetamine (1-2mg/kg, i.p.) administrations on alternate days in a test cage, either alone or combined with the glutamate N-methyl-D-aspartate receptor antagonist MK-801 (0.1-0.5mg/kg, i.p.). Seven days after treatment discontinuation, rats were re-exposed to the test cage to assess drug conditioning, and afterwards received a drug challenge. USVs and locomotor activity were evaluated, along with immunofluorescence for Zif-268 in various brain regions and spontaneous alternation in a Y maze. RESULTS:Amphetamine-treated rats displayed higher 50kHz USV emissions and locomotor activity than vehicle-treated rats, and emitted conditioned vocalizations on test cage re-exposure. Rats co-administered amphetamine and MK-801 displayed lower and dose-dependent 50kHz USV emissions, but not lower locomotor activity, during repeated treatment and challenge, and scarce conditioned vocalization compared with amphetamine-treated rats. These effects were associated with lower levels of Zif-268 after amphetamine challenge and spontaneous alternation deficits. CONCLUSIONS: These results indicate that glutamate transmission participates in the acute, long-term, and conditioned effects of amphetamine on 50kHz USVs, possibly by influencing amphetamine-induced long-term neuronal changes and/or amphetamine-associated memories

Polar bulges and polar nuclear discs: the case of NGC 4698

The early-type spiral NGC 4698 is known to host a nuclear disc of gas and stars which is rotating perpendicularly with respect to the galaxy main disc. In addition, the bulge and main disc are characterised by a remarkable geometrical decoupling. Indeed they appear elongated orthogonally to each other. In this work the complex structure of the galaxy is investigated by a detailed photometric decomposition of optical and near-infrared images. The intrinsic shape of the bulge was constrained from its apparent ellipticity, its twist angle with respect to the major axis of the main disc, and the inclination of the main disc. The bulge is actually elongated perpendicular to the main disc and it is equally likely to be triaxial or axisymmetric. The central surface brightness, scalelength, inclination, and position angle of the nuclear disc were derived by assuming it is infinitesimally thin and exponential. Its size, orientation, and location do not depend on the observed passband. These findings support a scenario in which the nuclear disc is the end result of the acquisition of external gas by the pre-existing triaxial bulge on the principal plane perpendicular to its shortest axis and perpendicular to the galaxy main disc. The subsequent star formation either occurred homogeneously all over the extension of the nuclear disc or through an inside-out process that ended more than 5 Gyr ago.Comment: 6 pages, 3 figures. Accepted for publication in MNRAS Letter

Accurate classification of 75 counterparts of objects detected in the 54 month Palermo Swift/BAT hard X-ray catalogue

Through an optical campaign performed at 4 telescopes located in the northern and the southern hemispheres, we have obtained optical spectroscopy for 75 counterparts of unclassified or poorly studied hard X-ray emitting objects detected with Swift/BAT and listed in the 54 month Palermo BAT catalogue. All these objects have also observations taken with Swift/XRT, ROSAT or Chandra satellites which allowed us to reduce the high energy error box and pinpoint the most likely optical counterpart/s. We find that 69 sources in our sample are Active Galactic Nuclei (AGNs); of them, 35 are classified as type 1 (with broad and narrow emission lines), 33 are classified as type 2 (with only narrow emission lines) and one is an high redshift QSO; the remaining 6 objects are galactic cataclysmic variables (CVs). Among type 1 AGNs, 32 are objects of intermediate Seyfert type (1.2-1.9) and one is Narrow Line Seyfert 1 galaxy; for 29 out of 35 type 1 AGNs, we have been able to estimate the central black hole mass and the Eddington ratio. Among type 2 AGNs, two display optical features typical of the LINER class, 3 are classified as transition objects, 1 is a starburst galaxy and 2 are instead X-ray bright, optically normal galaxies. All galaxies classified in this work are relatively nearby objects (0.006 - 0.213) except for one at redshift 1.137.Comment: 19 pages, 5 figures, 6 tables, accepted for publications on Astronomy and Astrophysics, main journal. arXiv admin note: text overlap with arXiv:1206.509

Hepatitis C virus genotypes in liver transplant recipients: Impact on posttransplant recurrence, infections, response to interferon-α therapy and outcome

Background. End-stage liver disease due to hepatitis C virus (HCV) is the most common indication for liver transplantation in U.S. veterans. We investigated the influence of HCV genotypes on the incidence and timing of recurrent HCV hepatitis, survival, infectious morbidity, and response to interferon-α therapy in this unique patient population. Methods. HCV genotype was determined by direct sequencing of the NS5 region of HCV with type-specific primers. Results. Genotype 1a (66%, 32/47) was the predominant genotype. Type 1b was found in 25% (12/47) of patients and type 2b was found in 9% (4/47). His topathologically recurrent HCV hepatitis developed in 53% (25/47) of the patients after transplantation. This group included 45% (14/31) of the patients with type 1a, 67% (8/12) of the patients with type 1b, and 25% (1/4) of the patients with type 2b (P>0.5). The time to recurrence and the severity of HCV recurrence as defined by aminotransferase levels or Knodell scores were not different among the three genotypes. There was a trend toward a higher incidence of major infections in patients with type 1b (75%) versus type 1a (48%) and type 2b (50%) (P=0.11). The response to interferon-α therapy did not differ significantly among the genotypes. Mortality at 5 years was 16% (5/31) in patients with genotype 1a, 42% (5/12) in patients with genotype 1b, and 50% (2/4) in patients with genotype 2b (P=0.06). Conclusions. The incidence, time to recurrence, and response to interferon-α therapy did not differ be tween the various genotypes in our liver transplant recipients. However, there was a trend toward higher infectious morbidity and overall mortality in patients with genotype 1b after transplantation

Collective modes of coupled phase oscillators with delayed coupling

We study the effects of delayed coupling on timing and pattern formation in spatially extended systems of dynamic oscillators. Starting from a discrete lattice of coupled oscillators, we derive a generic continuum theory for collective modes of long wavelength. We use this approach to study spatial phase profiles of cellular oscillators in the segmentation clock, a dynamic patterning system of vertebrate embryos. Collective wave patterns result from the interplay of coupling delays and moving boundary conditions. We show that the phase profiles of collective modes depend on coupling delays.Comment: 5 pages, 2 figure