GAME: Grb and All-sky Monitor Experiment

We describe the GRB and All-sky Monitor Experiment (GAME) mission submitted by a large international collaboration (Italy, Germany, Czech Repubblic, Slovenia, Brazil) in response to the 2012 ESA call for a small mission opportunity for a launch in 2017 and presently under further investigation for subsequent opportunities. The general scientific objective is to perform measurements of key importance for GRB science and to provide the wide astrophysical community of an advanced X-ray all-sky monitoring system. The proposed payload was based on silicon drift detectors (~1-50 keV), CdZnTe (CZT) detectors (~15-200 keV) and crystal scintillators in phoswich (NaI/CsI) configuration (~20 keV-20 MeV), three well established technologies, for a total weight of ~250 kg and a required power of ~240 W. Such instrumentation allows a unique, unprecedented and very powerful combination of large field of view (3-4 sr), a broad energy energy band extending from ~1 keV up to ~20 MeV, an energy resolution as good as ~300 eV in the 1-30 keV energy range, a source location accuracy of ~1 arcmin. The mission profile included a launch (e.g., by Vega) into a low Earth orbit, a baseline sky scanning mode plus pointed observations of regions of particular interest, data transmission to ground via X-band (4.8 Gb/orbit, Alcantara and Malindi ground stations), and prompt transmission of GRB / transient triggers.Comment: 13 pages, 8 figures, published in International Journal of Modern Physics

Carboplatin-Induced Bilateral Papilledema: A Case Report

We report on a patient with carboplatin-induced bilateral papilledema, as it was described in the 1970s for cisplatin. Loss of visual accuracy up to full blindness, often loss of color vision and scotomas can be seen as a result of cortical blindness, macula degeneration, retrobulbar neuritis and papilledema. These symptoms are mostly unilateral and initially mild, so that more chemotherapy is given before the diagnosis is made. The symptoms are usually reversible within weeks to months after cessation of the platinum treatment. The therapeutic strategy is stopping the platinum treatment. In addition the empiric use of corticosteroids is suggested

Study of Muscle Cell Dedifferentiation after Skeletal Muscle Injury of Mice with a Cre-Lox System

Background: Dedifferentiation of muscle cells in the tissue of mammals has yet to be observed. One of the challenges facing the study of skeletal muscle cell dedifferentiation is the availability of a reliable model that can confidentially distinguish differentiated cell populations of myotubes and non-fused mononuclear cells, including stem cells that can coexist within the population of cells being studied. Methodology/Principal Findings: In the current study, we created a Cre/Lox-β-galactosidase system, which can specifically tag differentiated multinuclear myotubes and myotube-generated mononuclear cells based on the activation of the marker gene, β-galactosidase. By using this system in an adult mouse model, we found that β-galactosidase positive mononuclear cells were generated from β-galactosidase positive multinuclear myofibers upon muscle injury. We also demonstrated that these mononuclear cells can develop into a variety of different muscle cell lineages, i.e., myoblasts, satellite cells, and muscle derived stem cells. Conclusions/Significance: These novel findings demonstrated, for the first time, that cellular dedifferentiation of skeletal muscle cells actually occurs in mammalian skeletal muscle following traumatic injury in vivo. © 2011 Mu et al

Simultaneous expression of Oct4 and genes of three germ layers in single cell-derived multipotent adult progenitor cells

Future application of adult stem cells in clinical therapies largely depends on the successful isolation of homogeneous stem cells with high plasticity. Multipotent adult progenitor cells (MAPCs) are thought to be a more primitive stem cell population capable of extensive in vitro proliferation with no senescence or loss of differentiation capability. The present study was aimed to find a less complicated and more economical protocol for obtaining single cell-derived MAPCs and understand the molecule mechanism of multi-lineage differentiation of MAPCs. We successfully obtained a comparatively homogeneous population of MAPCs and confirmed that single cell-derived MAPCs were able to transcribe Oct4 and genes of three germ layers simultaneously, and differentiate into multiple lineages. Our observations suggest that single cell-derived MAPCs under appropriate circumstances could maintain not only characteristics of stem cells but multi-lineage differentiation potential through quantitative modulation of corresponding regulating gene expression, rather than switching on expression of specific genes

The potential risks and impact of the start of the 2015–2016 influenza season in the WHO European Region: a rapid risk assessment

Background: Countries in the World Health Organization (WHO) European Region are reporting more severe influenza activity in the 2015–2016 season compared to previous seasons. Objectives: To conduct a rapid risk assessment to provide interim information on the severity of the current influenza season. Methods: Using the WHO manual for rapid risk assessment of acute public health events and surveillance data available from Flu News Europe, an assessment of the current influenza season from 28 September 2015 (week 40/2015) up to 31 January 2016 (week 04/2016) was made compared with the four previous seasons. Results: The current influenza season started around week 51/2015 with higher influenza activity reported in Eastern Europe compared to Western Europe. There is a strong predominance of influenza A(H1N1)pdm09 compared to previous seasons, but the virus is antigenically similar to the strain included in the seasonal influenza vaccine. Compared to the 2014/2015 season, there was a rapid increase in the number of severe cases in Eastern European countries with the majority of such cases occurring among adults aged < 65 years. Conclusions: The current influenza season is characterized by an early start in Eastern European countries, with indications of a more severe season. Currently circulating influenza A(H1N1)pdm09 viruses are antigenically similar to those included in the seasonal influenza vaccine, and the vaccine is expected to be effective. Authorities should provide information to the public and health providers about the current influenza season, recommendations for the treatment of severe disease and effective public health measures to prevent influenza transmission

Linked read technology for assembling large complex and polyploid genomes

Background: Short read DNA sequencing technologies have revolutionized genome assembly by providing high accuracy and throughput data at low cost. But it remains challenging to assemble short read data, particularly for large, complex and polyploid genomes. The linked read strategy has the potential to enhance the value of short reads for genome assembly because all reads originating from a single long molecule of DNA share a common barcode. However, the majority of studies to date that have employed linked reads were focused on human haplotype phasing and genome assembly. Results: Here we describe a de novo maize B73 genome assembly generated via linked read technology which contains ~ 172,000 scaffolds with an N50 of 89 kb that cover 50% of the genome. Based on comparisons to the B73 reference genome, 91% of linked read contigs are accurately assembled. Because it was possible to identify errors with \u3e 76% accuracy using machine learning, it may be possible to identify and potentially correct systematic errors. Complex polyploids represent one of the last grand challenges in genome assembly. Linked read technology was able to successfully resolve the two subgenomes of the recent allopolyploid, proso millet (Panicum miliaceum). Our assembly covers ~ 83% of the 1 Gb genome and consists of 30,819 scaffolds with an N50 of 912 kb. Conclusions: Our analysis provides a framework for future de novo genome assemblies using linked reads, and we suggest computational strategies that if implemented have the potential to further improve linked read assemblies, particularly for repetitive genomes

THESEUS::A key space mission concept for Multi-Messenger Astrophysics

The recent discovery of the electromagnetic counterpart of the gravitational wave source GW170817, has demonstrated the huge informative power of multi-messenger observations. During the next decade the nascent field of multi-messenger astronomy will mature significantly. Around 2030, third generation gravitational wave detectors will be roughly ten times more sensitive than the current ones. At the same time, neutrino detectors currently upgrading to multi km^3 telescopes, will include a 10 km^3 facility in the Southern hemisphere that is expected to be operational around 2030. In this review, we describe the most promising high frequency gravitational wave and neutrino sources that will be detected in the next two decades. In this context, we show the important role of the Transient High Energy Sky and Early Universe Surveyor (THESEUS), a mission concept proposed to ESA by a large international collaboration in response to the call for the Cosmic Vision Programme M5 missions. THESEUS aims at providing a substantial advancement in early Universe science as well as playing a fundamental role in multi-messenger and time-domain astrophysics, operating in strong synergy with future gravitational wave and neutrino detectors as well as major ground- and space-based telescopes. This review is an extension of the THESEUS white paper (Amati et al. 2017), also in light of the discovery of GW170817/GRB170817A that was announced on October 16th, 2017.Comment: 25 pages, 13 figures, accepted to Advances in Space Research with minor revisions. Details on the THESEUS instrumentation, science case and expected performances can also be found in Amati et al. 2017 (arXiv:1710.04638) and in the presentations of the THESEUS Workshop 2017 (http://www.isdc.unige.ch/theseus/workshop2017-programme.html). v2 few typos correcte

Incidence of SARS-CoV-2 in people with cystic fibrosis in Europe between February and June 2020

Background: Viral infections can cause significant morbidity in cystic fibrosis (CF). The current Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) pandemic could therefore have a serious impact on the health of people with CF (pwCF). Methods: We used the 38-country European Cystic Fibrosis Society Patient Registry (ECFSPR) to collect case data about pwCF and SARS-CoV-2 infection. Results: Up to 30 June 2020, 16 countries reported 130 SARS-CoV-2 cases in people with CF, yielding an incidence of 2.70/1000 pwCF. Incidence was higher in lung-transplanted patients (n=23) versus non-transplanted patients (n=107) (8.43 versus 2.36 cases/1000). Incidence was higher in pwCF versus the age-matched general population in the age groups &lt;15, 15-24, and 25-49 years (p&lt;0.001), with similar trends for pwCF with and without lung transplant. Compared to the general population, pwCF (regardless of transplantation status) had significantly higher rates of admission to hospital for all age groups with available data, and higher rates of intensive care, although not statistically significant. Most pwCF recovered (96.2%), however 5 died, of whom 3 were lung transplant recipients. The case fatality rate for pwCF (3.85%, 95% CI: 1.26-8.75) was non-significantly lower than that of the general population (7.46%; p=0.133). Conclusions: SARS-CoV-2 infection can result in severe illness and death for pwCF, even for younger patients and especially for lung transplant recipients. PwCF should continue to shield from infection and should be prioritized for vaccination