Copper effect on the protein composition of photosystem II

The definitive version is available at: http://www.blackwell-synergy.com/doi/full/10.1111/j.1399-3054.2000.1100419.xWe provide data from in vitro experiments on the polypeptide composition, photosynthetic electron transport and oxygen evolution activity of intact photosystem II (PSII) preparations under Cu(II) toxicity conditions. Low Cu(II) concentrations (Cu(II) per PSII reaction centre unit≤230) that caused around 50% inhibition of variable chlorophyll a fluorescence and oxygen evolution activity did not affect the polypeptide composition of PSII. However, the extrinsic proteins of 33, 24 and 17 kDa of the oxygen-evolving complex of PSII were removed when samples were treated with 300 μM CuCl2 (Cu(II) per PSII reaction centre unit=1 400). The LHCII antenna complex and D1 protein of the reaction centre of PSII were not affected even at these Cu(II) concentrations. The results indicated that the initial inhibition of the PSII electron transport and oxygen-evolving activity induced by the presence of toxic Cu(II) concentrations occurred before the damage of the oxygen-evolving complex. Indeed, more than 50% inhibition could be achieved in conditions where its protein composition and integrity was apparently preserved.This work was supported by the Dirección General de Investigación Científica y Técnica (Grant PB98-1632).Peer reviewe

Interleukin 10 inhibits pro-inflammatory cytokine responses and killing of Burkholderia pseudomallei.

Melioidosis, caused by Burkholderia pseudomallei, is endemic in northeastern Thailand and Northern Australia. Severe septicemic melioidosis is associated with high levels of pro-inflammatory cytokines and is correlated with poor clinical outcomes. IL-10 is an immunoregulatory cytokine, which in other infections can control the expression of pro-inflammatory cytokines, but its role in melioidosis has not been addressed. Here, whole blood of healthy seropositive individuals (n = 75), living in N. E. Thailand was co-cultured with B. pseudomallei and production of IL-10 and IFN-γ detected and the cellular sources identified. CD3- CD14+ monocytes were the main source of IL-10. Neutralization of IL-10 increased IFN-γ, IL-6 and TNF-α production and improved bacteria killing. IFN-γ production and microbicidal activity were impaired in individuals with diabetes mellitus (DM). In contrast, IL-10 production was unimpaired in individuals with DM, resulting in an IL-10 dominant cytokine balance. Neutralization of IL-10 restored the IFN-γ response of individuals with DM to similar levels observed in healthy individuals and improved killing of B. pseudomallei in vitro. These results demonstrate that monocyte derived IL-10 acts to inhibit potentially protective cell mediated immune responses against B. pseudomallei, but may also moderate the pathological effects of excessive cytokine production during sepsis

Experiences of dementia in a foreign country: qualitative content analysis of interviews with people with dementia

Background: Dementia is a worldwide health concern of epidemic proportions. Research in the field of subjective experience of dementia suffers from a lack of diversity of their participants including immigrants. Different portraits of life with dementia could help us understand how people with dementia conceptualise their experiences of dementia and how they live. Our study aimed to explore the subjective experiences of living with dementia among Iranian immigrants in Sweden. Methods: Qualitative content analysis of interviews with fifteen people with dementia from Iranian immigrant backgrounds were conducted (8 females and 7 males). Results: Three themes and seven associated sub-themes were revealed. The themes included: Being a person with dementia means living with forgetfulness (personal sphere), living with forgetfulness in the private sphere means feeling incompetent but still loved, living with forgetfulness in the public sphere means feeling confident and secure but also isolated. Conclusions: Living with dementia for the participants meant living with forgetfulness. They experienced feeling incompetent but still loved within their families and feeling confident and secure but also isolated in the society. Educating people with dementia and their families about the course and process of dementia may help them understand the changes better and adjust their expectations. Our study can provide a basis for healthcare workers to understand the experiences of living with dementia from this specific perspective

Direct Simulation of a Solidification Benchmark Experiment

International audienceA solidification benchmark experiment is simulated using a three-dimensional cellular automaton-finite element solidification model. The experiment consists of a rectangular cavity containing a Sn-3 wt pct Pb alloy. The alloy is first melted and then solidified in the cavity. A dense array of thermocouples permits monitoring of temperatures in the cavity and in the heat exchangers surrounding the cavity. After solidification, the grain structure is revealed by metallography. X-ray radiography and inductively coupled plasma spectrometry are also conducted to access a distribution map of Pb, or macrosegregation map. The solidification model consists of solutions for heat, solute mass, and momentum conservations using the finite element method. It is coupled with a description of the development of grain structure using the cellular automaton method. A careful and direct comparison with experimental results is possible thanks to boundary conditions deduced from the temperature measurements, as well as a careful choice of the values of the material properties for simulation. Results show that the temperature maps and the macrosegregation map can only be approached with a three-dimensional simulation that includes the description of the grain structure

Diversity of Staphylococcus aureus Isolates in European Wildlife

Staphylococcus aureus is a well-known colonizer and cause of infection among animals and it has been described from numerous domestic and wild animal species. The aim of the present study was to investigate the molecular epidemiology of S. aureus in a convenience sample of European wildlife and to review what previously has been observed in the subject field. 124 S. aureus isolates were collected from wildlife in Germany, Austria and Sweden; they were characterized by DNA microarray hybridization and, for isolates with novel hybridization patterns, by multilocus sequence typing (MLST). The isolates were assigned to 29 clonal complexes and singleton sequence types (CC1, CC5, CC6, CC7, CC8, CC9, CC12, CC15, CC22, CC25, CC30, CC49, CC59, CC88, CC97, CC130, CC133, CC398, ST425, CC599, CC692, CC707, ST890, CC1956, ST2425, CC2671, ST2691, CC2767 and ST2963), some of which (ST2425, ST2691, ST2963) were not described previously. Resistance rates in wildlife strains were rather low and mecA-MRSA isolates were rare (n = 6). mecC-MRSA (n = 8) were identified from a fox, a fallow deer, hares and hedgehogs. The common cattle- associated lineages CC479 and CC705 were not detected in wildlife in the present study while, in contrast, a third common cattle lineage, CC97, was found to be common among cervids. No Staphylococcus argenteus or Staphylococcus schweitzeri-like isolates were found. Systematic studies are required to monitor the possible transmission of human- and livestock- associated S. aureus/MRSA to wildlife and vice versa as well as the possible transmission, by unprotected contact to animals. The prevalence of S. aureus/MRSA in wildlife as well as its population structures in different wildlife host species warrants further investigation

Identifikasi Pangan Lokal di Hunian dan Sosialisasi Penanganan Pangan Terhadap Hama Kutu Sitophilus Zea mays L. dengan Pestisida Alami

Research and devocation carried out the identification of the community aims to local food and socialization prevention as well as treatment of pest infestasion Sitophilus zea mays L. with natural pesticide, this activity carried out Bikekneno in the village, in South Mollo, Regency South-Central Timor, East Nusa Tenggara province. The event was held in a survey, socialization anda research into prevention as well as to pest infestation handling Sitophilus zea mays L. with natural pesticide. Based on the research food in the form of a corn plant food followed (59,6%) nut rice, arbila (19,0%), rice (13%), peanut (13%), green bean (12%), long beans (3%), squash wax (1%), and mos low (0%)beans maize. From this data be said that the village Bikekneno people food still in the form of a corn plant as prime food. Backup storage of food to villagers Bikekneno mostly kept in the furnance of fire found houselocal furnance. The food was influential storage pest shed on the growth oftenresulting in damage to food after people to harvest in the garden. If corn therefore a socialization of prevention as well as treatment of pest infestation Sitophilus zea mays L. With pesticide know the leaves of the pandanus, colored leaves, breadfruit leaves and the regulation of temperature in the good Bikekneno storage reserve in the villagePenelitian dan pengabdian masyarakat ini bertujuan untuk melakukan identifikasi pangan lokal dan sosialisasi pencegahan serta penanganan terhadap hama kutu Sitophilus zea mays l. dengan pestisida alami, Kegiatan ini dilaksanankan di Desa Bikekneno, Kecamatan Mollo Selatan, Kabupaten Timor Tengah Selatan, Provinsi Nusa Tenggara Timur. Kegiatan ini dilaksanakan menggunakan metode survey, sosialisasi dan riset tentang pencegahan serta penangan terhadap hama kutu Sitophilus zea mays l. dengan pestisida alami. Berdasarkan hasil penelitian masyarakat Desa Bikekneno memiliki potensi pangan lokal yang sangat besar. Masyarakat Desa Bikekneno 76,4% menyimpan cadangan makanan berupa tanaman jagung diikuti pangan Kacang Nasi (59,6%), Arbila (19,0%), Padi (13%), Kacang Tanah (13%), Kacang Hijau (12%), kacang panjang (3%), Labu lilin (1%) dan yang paling rendah yakni Kacang Jagung (0%). Dari data ini dapat dikatakan bahwa hasil pangan di masyakat desa Bikekneno masih berupa tanaman jagung sebagai pangan utama. Penyimpanan cadangan makanan pada masyarakat Desa Bikekneno sebagian besar disimpan di dapur Api tungku lokal (Rumah Bulat). Tempat peyimpanan pangan ini berpengaruh terhadap tingkat pertumbuhan hama gudang yang sering mengakibatkan kerusakan pada pangan setelah masyarakat melakukan panen di kebun. Jika masyarakat desa salah dalam menyimpan hasil panen pangan mereka maka pangan akan rusak oleh hama kutu jagung (Sitophylus zeamays). Oleh sebab itu dilakukan Sosialisasi dan simulasi tentang pencegahan serta penanganan terhadap hama kutu Sitophilus zea mays l.dengan petsisida alami berupa daun pandan, daun jambu, daun sukun dan pengaturan suhu yang baik pada tempat menyimpanan cadangan makanan di Desa Bikeknen

Роль інформатизації освітнього процесу для якісної підготовки фахівців

У статті розкрито роль інформатизації навчального процесу для якісної підготовки фахівців для досягнення в освітньому процесі системного бачення майбутньої інноваційної діяльності спеціалістів, сприйняття її як єдиної системи професійних функцій і завдань, віддаючи перевагу до інформатизації. Метою статті є з’ясувати роль інформатизації навчального процесу для якісної підготовки фахівців для досягнення системного бачення майбутньої інноваційної діяльності в освітньому процесі, сприйняття її як єдиної системи професійних функцій і завдань. , надаючи перевагу інформатизації. Методична концепція наукової підготовки майбутніх фахівців в умовах інформатизації навчального процесу для якісної підготовки фахівців відображає взаємозв’язок і взаємодію наукових підходів до дослідження фундаментальної проблеми. Інформатизація навчального процесу розглядалася в аспектах: системному, діяльнісному та інформаційному. Представлено програмні засоби для організації ефективного процесу інформатизації освіти для якісної підготовки фахівців. Визначено позитивні фактори інформатизації освітнього процесу, що підвищують ефективність підготовки здобувачів освіти та фактори, що сприяють оперативному оновленню та вирішенню завдань, наповненню змісту освітнього середовища шляхом застосування нових технологій.The article reveals the role of informatization of the educational process for high-quality training of specialists in order to achieve in the educational process a systemic vision of the future innovative activity of specialists, its perception as a single system of professional functions and tasks, giving preference to informatization. The purpose of the article is to clarify the role of informatization of the educational process for quality training of specialists to achieve a systemic vision of future innovative activity in the educational process, its perception as a single system of professional functions and tasks. , giving preference to informatization. The methodical concept of scientific training of future specialists in the conditions of informatization of the educational process for high-quality training of specialists reflects the relationship and interaction of scientific approaches to the study of a fundamental problem. Informatization of the educational process was considered in aspects

ВЕГЕТАТИВНІ РОЗЛАДИ ПРИ ХРОНІЧНОМУ ПАНКРЕАТИТІ ТА ПОЄДНАНИХ ШЛУНКОВО-КИШКОВИХ СТАНАХ

The article analyzes the literature on the role of autonomic dysfunction and other psychosomatic disorders in causing and progression of pancreatitis and associated gastrointestinal states. Investigated the influence of deviations balance regulation autonomic nervous system to the emergence of hemodynamic, metabolic, energy disorders in aforementioned pathological conditions.В статье проанализированы литературные источники о роли вегетативных дисфункций и других психосоматических расстройств в возникновении и течении панкреатита и объединенных желудочно-кишечных состояний. Исследуется влияние отклонения баланса регуляции вегетативной нервной системой появления гемодинамических, метаболических, энергетических нарушений при вышеуказанных патологических состояниях. У статті проаналізовано літературні джерела щодо ролі вегетативних дисфункцій та інших психосоматичних розладів у виникненні та перебігу панкреатиту і поєднаних шлунково-кишкових станів. Досліджується вплив відхилення балансу регуляції вегетативною нервовою системою на появу гемодинамічних, метаболічних, енергетичних порушень при вищевказаних патологічних станах.

Pelatihan Tata Cara Submit Artikel Melalui Open Journal System (OJS) Bagi Mahasiswa

Publikasi artikel ilmiah merupakan salah satu syarat kelulusan bagi mahasiswa program studi peternakan, banyak mahasiswa yang belum memahami secara benar tata cara submit artikel di jurnal ilmiah menggunakan Open Journal System (OJS), sehingga pelatihan ini dilakukan untuk membantu mahasiswa memahami cara mendaftar dan submit artikel ke OJS. Pelatihan ini telah dilaksanakan di Laboratorium Lapangan Lahan Kering Universitas Nusa Cendana pada tanggal 8 Februari 2024 Pukul 10.00 WITA, dan selesai pukul 12.00 WITA. Kegiatan ini dihadiri oleh mahasiswa semester 6 dan 8 sejumlah 16 orang. Pelatihan meliputi penjelasan tentang cara penulisan artikel, dan submit artikel di jurnal ilmiah menggunakan Open Journal System secara benar. Sebelum pelatihan dilakukan peserta ditanyakan terkait penulisan artikel, dan cara submit jurnal menggunakan OJS. Hal ini untuk mengukur pemahaman mahasiswa terkait materi yang disampaikan. Metode yang digunakan dalam pelatihan ini adalah pengenalan sistem OJS, dan dalam pelatihan artikel ilmiah dikirimkan melalui OJS. Pelatihan penyampaian artikel penelitian melalui OJS Selain penerapan sistem OJS, kegiatan tahap selanjutnya adalah pelatihan penyampaian artikel penelitian melalui OJS. Tujuannya adalah untuk membekali mahasiswa dengan pengetahuan dan keterampilan menulis dan mencatat makalah penelitian serta menyerahkan makalah penelitian melalui OJS. Selain itu tim pengabdi juga memberikan bimbingan kepada mahasiswa seperti formulir evaluasi untuk mengetahui apakah mahasiswa sudah memahami dengan benar cara submit artikel melalui OJS. Berdasarkan hasil kegiatan yang telah dilakukan, dapat dilihat bahwa dari total mahasiswa yang mengikuti kegiatan ini sebanyak 62,5% peserta sangat paham terhadap penjelasan yang disampaikan, 25% paham, sedangkan 12,5% kurang paham. Bimbingan dilakukan sejak artikel mahasiswa berhasil disubmit sampai artikel mahasiswa tersebut dinyatakan telah terbit pada salah satu jurnal. Pembimbingan dilakukan untuk mentransfer pengetahuan serta wawasan bagi mahasiswa, sehingga mahasiswa mampu memahami banyak tingkatan yang perlu dimengerti dalam penulisan, dan publikasi artikel