Altered bactericidal activity aginst staphylococcus aureus in tuberculous bronchoalveolr lavage fluids

Altered bactericidal activity against Staphylococcus aureus in tuberculous bronchoalveolar lavage fluids. P. Selvaraj, N. Venkataprasad, V.K. Vijayan, P.R. Narayanan. ©ERS Journals Ltd 1994. ABSTRACT: We wished to evaluate the pulmonary defence capacity against common bacterial infections in patients with pulmonary tuberculosis. Bactericidal activity against Staphylococcus aureus of bronchoalveolar lavage fluids (cell-free supernatants) of patients with active (n=13) and inactive pulmonary tuberculosis (n=8), and normal individuals (n=6), were studied. The 2 and 4 h bactericidal activities were higher than the 0 h activity in lavage fluids of healthy subjects and patients with inactive pulmonary tuberculosis. Active tuberculous lavage fluids were equally competent in their bactericidal activity against S. aureus at 0 and 2 h, but a reduced S. aureus hilling was seen at 4 h of incubation. Estimation of total phospholipid levels revealed no significant difference between the various lavage fluids. This reduced hilling of S. aureus showed a relationship with the cellular components (lymphocytes and macrophages) of active tuberculous lavage fluids. A reduced hilling was associated with no lymphocytic alveolitis, and an increased hilling with lymphocytic alveolitis. This study suggests that alveolar lining material of patients with active pulmonary tuberculosis has less bactericidal activity against bacterial infections, such as S. aureus

Alveolar macrophages in patients with pulmonary tuberculosis

Total and HLA-DR positive alveolar macrophages were enumerated in the broncho-alveolar lavage fluids of active pulmonary tuberculosis, inactive pulmonary tuberculosis (treated cases), nontuberculous lung disease patients and normal healthy individuals. A significantly increased number of macrophages was found in the patient groups than the normal individuals: however, there was no difference in HLA-DR positive cells. Among the different patient groups, smokers had more macrophages as well as more HLA-DR positive macrophages than non-smokers and normal subjects Total number of alveolar macrophages of the active tuberculosis non-smoker group was significantly higher than the inactive tuberculosis non-smoker group and normal individuals. On the contrary, a slightly decreased number of HLA-DR positive cells was seen in the non-smoker group of active-TB patients. The present study suggests that infection and smoking induce the accumulation of macrophages in the lung. The implication of these findings in relation to tuberculosis is discussed

Procoagulant activity of bronchoalveolar lavage fluids taken from the site of tuberculous lesions

Procoagulant activity of bronchoalveolar lavage fluids taken from the site of tuberculous lesions. P. Selvaraj, N. Venkataprasad, V.K. Vijayan, R Prabhakar, P.R Narayanan. ©ERS Journals Ltd 1991. ABSTRACT: We wanted to determine the procoagulant activity (PCA) of bronchoalveolar lavage fluids, in order to understand the macrophage-mediated lung injury at the site of tuberculous lesion. Alveolar lavage fluids taken from the site of a lesion (radiologically abnormal site (RAS)) and an unaffected site (radiologically normal site (RNS)) of active pulmonary tuberculosis (TB) patients (n=7) and inactive (cured) patients (n=9) were studied for their PCA producing potential. The observed results were not significant using Mann-Whitney test, and thus all increases/decreases reported below are trends/ tendencies only. An increased PCA was seen in 4 out of 7 cell-free lavage supernatants of active- TB taken from the site of lesion (RAS), compared to only 1 out of 9 in inactive- TB. The PCA producing potential of the alveolar macrophages of RAS and RNS of active-TB patients was enhanced when the alveolar macrophages were cocultured with autologous peripheral blood lymphocytes under in vitro condition. Stimulation with purified protein derivative (PPD) of M. tuberculosis showed a variable (increased or decreased) PCA production. Peripheral blood monocytes and total mononuclear cells (monocytes + lymphocytes) of active-TB patients stimulated with or without PPD showed increased PCA production, compared with normal individuals and inactive-TB patients. The present study suggests that increased production of PCA by the alveolar macrophages, in collaboration with lymphocytes and other cells at the site of tuberculous lesions will result in fibrin formation. The deposition of fibrin in the alveoli may lead to further lung injury

Research and development in marine fisheries extension in Tamil Nadu

improvement In the standard of living of fishing households and fishing labor households and optimal utilisation of fishery resources for employment, production and export are the Important goals of marine fisheries extension service. Despite ttie increasing attention of the Government to fisheries extension work, majority of fisherfolk are caught In a vicious cycle of poverty and indebtness. The paper analyses the present strategies of fisheries extension service In Tamil Nadu, Identifies the pitfalls and lists suggestions for Improving the efficiency of fisheries extension work In the state. The extension teaching methods used for Individual contact, group contact and mass contact, the sul)Ject-matter fields covered In extension teaching with their relative Importance. The characteristics and extension personnel, research support provided, coordination among the various agencies Involved and participation of the clientele and their organisations are analysed and discussed. The analysis helped identifying the major Impediments to success of extension efforts. The analysis provided a tasks for evolving future extension strategies focussed on marine fisheries development. It provides suggestions in term of personnel requirement, effective extension methods, thrust areas among different subject matter- fields, co-ordination among organisations concerned with marine fisheries and extension research support. The recommendations will be of use to the organisations concerned with fisheries extension In their approach to tone up the service of fisheries extension

Vitamin D receptor and interleukin-1 receptor antagonist gene polymorphism in spinal tuberculosis

Our earlier studies revealed that both MHC (Major Histocomptibility Complex) and non-MHC genes are associated with the susceptibility to pulmonary tuberculosis (TB). To find out whether non-MHC genes such as vitamin D receptor (VDR) and interleukin-1 receptor antagonist (IL-1RA) genes are associated with the susceptibility to spinal TB (extrapulmonary form of TB), the present study was carried out in spinal TB patients (n=66) and spouses of TB patients (spinal-TB and pulmonary-TB) ( n = 80) (family contacts). A trend towards an increased per cent genotype frequency of IL-1RA genotype variant 22 (12.1%) was seen in spinal TB patients when compared to the controls (3.8%) (spouses of the patients) (P=0.057; odds ratio 3.5). No difference was observed in the frequency of VDR genotypes between the overall spinal TB patients and the family contacts. However, the VDR mutant genotype tt was seen at a higher frequency in female patients with TB spine (TBS) (12.8%) than female contacts (4.2%) ( P >0.05 not significant; odds ratio 3.5). Among the contacts, a significantly increased frequency of wild type genotype TT (wild homozygotes) was seen in female contacts (55.1%) than male contacts (16.1%) (P =0.0012). Similarly a significant decrease in tt genotype was seen in female contacts (4.1%) than male contacts (25.8%) (P=0.012). The present study suggests that IL-1RA genotype 22 may be associated with the susceptibility to spinal TB. Moreover, vitamin D receptor tt genotype may be associated with the susceptibility to spinal TB in female patients. The study reveals that multicandidate genes may be associated with the susceptibility to spinal TB

HLA-DR phenotypes and lymphocyte response to M. tuberculosis antigens and in cured spinal tuberculosis patients and their contacts

Background: Our earlier studies on Human Leucocyte Antigens (HLA) in pulmonary tuberculosis patients revealed the association of HLA-DR2 antigen with susceptibility to pulmonary TB and DR2 antigen has been shown to influence the immunity to tuberculosis. Objectives: The present study was carried out to find out whether HLA-DR antigens are associated with susceptibility to spinal tuberculosis. Moreover, the role of HLA-DR antigens on lymphoproliferative response to Mycobacterium tuberculosis culture filtrate antigens was studied using Lymphocyte Transformation Test (LTT). Material and Methods: HLA-DR genotyping and lymphoproliferative response was carried out in 63 cured spinal TB patients and 63 control subjects (spouses of pulmonary and spinal TB patients). Results: A trend towards an increased frequency of HLA-DR9 antigen was observed in spinal TB patients compared to controls. A significantly decreased lymphocyte response to M. tuberculosis antigens was observed in HLA-DR9 antigen positive control subjects compared to HLA- DR9 antigen negative subjects (P=0.0009) whereas increased response was observed with DR9 positive cured spinal TB patients compared to HLA-DR9 antigen negative patients. Further, HLADR3 antigen positive patients showed a decreased lymphocyte response compared to HLA-DR3 antigen negative patients (P<0.05). Conclusion: The study suggests that HLA-DR9 antigen either alone or in combination with other HLA antigen as lhplotype and non-HLA genes may be associated with susceptibility to spinal TB and play a regulatory role on the immune response to M. tuberculosis in spinal tuberculosis patients

Discovery and Development of Small Molecule Allosteric Modulators of Glycoprotein Hormone Receptors

Glycoprotein hormones, follicle-stimulating hormone (FSH), luteinizing hormone (LH), and thyroid stimulating hormone (TSH) are heterodimeric proteins with a common subunit and hormone-specific subunit. These hormones are dominant regulators of reproduction and metabolic processes. Receptors for the glycoprotein hormones belong to the family of G-protein coupled receptors (GPCR). FSH receptor (FSHR) and LH receptor (LHR) are primarily expressed in somatic cells in ovary and testis to promote egg and sperm production in women & men respectively. TSH receptor (TSHR) is expressed in thyroid cells and regulates the secretion of T3 & T4. Glycoprotein hormones bind to the large extracellular domain of the receptor and cause a conformational change in the receptor that leads to activation of more than one intracellular signaling pathway. Several small molecules have been described to activate/inhibit glycoprotein hormone receptors through allosteric sites of the receptor. Small molecule allosteric modulators have the potential to be administered orally to patients thus improving the convenience of treatment. It has been a challenge to develop a small molecule allosteric agonist for glycoprotein hormones that can mimic the agonistic effects of the large natural ligand to activate similar signaling pathways. However, in the past few years, there have been several promising reports describing distinct chemical series with improved potency in preclinical models. In parallel, proposal of new structural model for FSH receptor and in silico docking studies of small molecule ligands to glycoprotein hormone receptors provide a giant leap on the understanding of the mechanism of action of the natural ligands and new chemical entities on the receptors. This review will focus on the current status of small molecule allosteric modulators of glycoprotein hormone receptors, their effects on common signaling pathways in cells, their utility for clinical application as demonstrated in preclinical models, and use of these molecules as novel tools to dissect the molecular signaling pathways of these receptors

Collaborative decision making in complex work settings: a process of managing inter dependencies

There exists disparity between the conceptualization and occurrence of Collaborative Decision Making (CDM) in everyday work activities of complex work settings. Current notions in the field of Computer Supported Cooperative Work (CSCW) based on studies of decision making in groups typically portray CDM as an isolated event in which multiple personnel jointly undertake decision making. In the real world, however, decisions are made during work performance and interlaced with other processes and activities. Moreover, the complex work setting is a cooperative arrangement in which decision making is distributed. This research aims to alleviate the disparity by investigating how people in a complex working environment make decisions collaboratively. The original contribution to knowledge made by this thesis is the theory of CDM as a process of managing interdependencies. Field-studies conducted in an airport to examine the way CDM is undertaken during Air Traffic Control operations inform theory development. The study takes a qualitative approach and is guided by Grounded Theory Methodology (GTM). The findings of this research indicate that undertaking decision making in the cooperative arrangement of complex work settings requires managing the distributions and interconnections inherent in this setup. In addition, participation and contribution of personnel in decision making is found to be structured by the dependencies between their activities. These findings form the central focus of the theory leading to the depiction of CDM as a process of managing interdependencies. The theory presented in this thesis clarifies and extends existing views by explicating the differentiated process of CDM in the cooperative arrangement of a complex work setting. Based on this a new definition of CDM is formulated. In addition, a conceptual framework of ten parameters is derived to serve as a tool for analysing CDM taking place in a particular work setting. Application of this framework is demonstrated by analysing an aircraft accident report to draw insights about the occurrence of CDM in this setting

Seasonal changes in growth & alginic acid & mannitol contents in Sargassum ilicifolium (Turner) J. agardh & S. myriocystum J. agardh

Young plants of S. ilicifolium and S. myricosystum appear in April and May and reach the maximum size in September or October. Alginic acid yield varies with the seasonal growth behaviour of these alginophytes. with maximum yield in July or August. Alginic acid content varies from 22.3 to 30.8% in S. ilieifolium and from 15.9 to 34.5% in S. myriocyslum. Mannitol content ranges from 2 to 5 and 1.3 to 5% in S. jlicifolium and S. myriocysmm respectively. The suitable harv.esting period for getting the maximum yidd of algin ic acid appears to be between July and September