Eic1 links Mis18 with the CCAN/Mis6/Ctf19 complex to promote CENP-A assembly.

CENP-A chromatin forms the foundation for kinetochore assembly. Replication-independent incorporation of CENP-A at centromeres depends on its chaperone HJURP(Scm3), and Mis18 in vertebrates and fission yeast. The recruitment of Mis18 and HJURP(Scm3) to centromeres is cell cycle regulated. Vertebrate Mis18 associates with Mis18BP1(KNL2), which is critical for the recruitment of Mis18 and HJURP(Scm3). We identify two novel fission yeast Mis18-interacting proteins (Eic1 and Eic2), components of the Mis18 complex. Eic1 is essential to maintain Cnp1(CENP-A) at centromeres and is crucial for kinetochore integrity; Eic2 is dispensable. Eic1 also associates with Fta7(CENP-Q/Okp1), Cnl2(Nkp2) and Mal2(CENP-O/Mcm21), components of the constitutive CCAN/Mis6/Ctf19 complex. No Mis18BP1(KNL2) orthologue has been identified in fission yeast, consequently it remains unknown how the key Cnp1(CENP-A) loading factor Mis18 is recruited. Our findings suggest that Eic1 serves a function analogous to that of Mis18BP1(KNL2), thus representing the functional counterpart of Mis18BP1(KNL2) in fission yeast that connects with a module within the CCAN/Mis6/Ctf19 complex to allow the temporally regulated recruitment of the Mis18/Scm3(HJURP) Cnp1(CENP-A) loading factors. The novel interactions identified between CENP-A loading factors and the CCAN/Mis6/Ctf19 complex are likely to also contribute to CENP-A maintenance in other organisms.L.S. was supported by an EC FP7 Marie Curie International Incoming Fellowship (PIIF-GA-2010-275280) and an EMBO Long Term Fellowship (ALTF 1491-2010). The Darwin Trust and a Principal's Career Development scholarship supported N.R.T.T. The Wellcome Trust supported the work of R.C.A. (095021 and 065061) and J.R. (084229) along with funding from the European Commission Network of Excellence EpiGeneSys (HEALTH-F4-2010-257082) to R.C.A. The Wellcome Trust Centre for Cell Biology (092076) and mass spectrometry instrumentation (091020) are supported by funding from the Wellcome Trust. R.C.A. is a Wellcome Trust Principal Research Fellow

Visual Associative Learning in Restrained Honey Bees with Intact Antennae

A restrained honey bee can be trained to extend its proboscis in response to the pairing of an odor with a sucrose reward, a form of olfactory associative learning referred to as the proboscis extension response (PER). Although the ability of flying honey bees to respond to visual cues is well-established, associative visual learning in restrained honey bees has been challenging to demonstrate. Those few groups that have documented vision-based PER have reported that removing the antennae prior to training is a prerequisite for learning. Here we report, for a simple visual learning task, the first successful performance by restrained honey bees with intact antennae. Honey bee foragers were trained on a differential visual association task by pairing the presentation of a blue light with a sucrose reward and leaving the presentation of a green light unrewarded. A negative correlation was found between age of foragers and their performance in the visual PER task. Using the adaptations to the traditional PER task outlined here, future studies can exploit pharmacological and physiological techniques to explore the neural circuit basis of visual learning in the honey bee

... Ford's Theatre Society presents ... William Shakespeare's agreeable jest ... The Comedy of errors. [Wash.] NRT-at-Ford's Theatre, 1968.

Playbill in folder, printed in double columns on each side.; GB1/17/69.Page Order: Multipag

... Ford's Threatre Society presents ... Stephen Vincent Benet's Civil War saga ... John Brown's body. [Wash.] NRT-at-Ford's Theatre, 1968.

Playbill in folder, printed in double columns on each side. Prepared for opening night of NRT-at-Ford's Theatre, February 12, 1968.; GB1/17/69.Page Order: Multipag

... Ford's Theatre Society presents ... Oliver Goldmisth's lusty masterpiece ... She stoops to conquer, or, the Mistakes of a night. [Wash.] NRT-at-Ford's Theatre, 1968.

Playbill in folder, printed in double columns on each side.; GB1/17/69.Page Order: Multipag