Viscosity dependence of the rotational reorientation of coumarins by depolarization technique

Steady-​state and time-​resolved fluorescence behavior of coumarin 6 (C6) and coumarin 7 (C7) in alkane and alc. solvents has been investigated. Steady-​state absorption and emission spectral behavior of C6 and C7 was found not to be affected by both the solvents. Time-​resolved fluorescence anisotropy measurements showed that the rotational reorientation of the probe became faster in alc. A comparison of the exptl. measured rotational reorientation times of C6 and C7 were identical in a given solvent at a particular temp. The present study has been undertaken to examine the role of friction experienced by the polar solutes in a polar and non-​polar solvents. Mol. shape and size were similar but the friction experienced by these probes in n-​tridecane and n-​propanol solvents viscosities were varied by temp. However, it was obsd. that C6 and C7 rotates faster in alc. than alkane and the obsd. results were discussed in the last section

An Experimental and Computational Study of 2-(3-Oxo-3H-benzof chromen-1-ylmethoxy)-Benzoic Acid Methyl Ester

The various methods for studying polarities are based on the use of probe molecules, whose molecular spectral profile is significantly affected by the polarity of the medium. The absorption and emission spectra and dipole moments (µg and µe) of 2-(3-oxo-3H-benzofchromen-1-ylmethoxy)-benzoic acid methyl ester (2BME) are studied in solvents of different polarities at room temperature. The determination of dipole moments by solvatochromic shift using various relations and the change in dipole moment (�µ) were determined using Stokes shift with the variation of the solvent polarity parameter (ET N). The value of µe greater than µg indicating that the probe is more polar in the higher state. DFT and TDDFT theoretical analysis of dipole moment in the vacuum and with solvent, solvent accessible surface (SAS) and molecular electrostatic potential (MEP) are also performed. © 2017, Springer Science+Business Media, LLC

Activity map of the tammar X chromosome shows that marsupial X inactivation is incomplete and escape is stochastic

BACKGROUND: X chromosome inactivation is a spectacular example of epigenetic silencing. In order to deduce how this complex system evolved, we examined X inactivation in a model marsupial, the tammar wallaby (Macropus eugenii). In marsupials, X inactivation is known to be paternal, incomplete and tissue-specific, and occurs in the absence of an XIST orthologue. RESULTS: We examined expression of X-borne genes using quantitative PCR, revealing a range of dosage compensation for different loci. To assess the frequency of 1X- or 2X-active fibroblasts, we investigated expression of 32 X-borne genes at the cellular level using RNA-FISH. In female fibroblasts, two-color RNA-FISH showed that genes were coordinately expressed from the same X (active X) in nuclei in which both loci were inactivated. However, loci on the other X escape inactivation independently, with each locus showing a characteristic frequency of 1X-active and 2X-active nuclei, equivalent to stochastic escape. We constructed an activity map of the tammar wallaby inactive X chromosome, which identified no relationship between gene location and extent of inactivation, nor any correlation with the presence or absence of a Y-borne paralog. CONCLUSIONS: In the tammar wallaby, one X (presumed to be maternal) is expressed in all cells, but genes on the other (paternal) X escape inactivation independently and at characteristic frequencies. The paternal and incomplete X chromosome inactivation in marsupials, with stochastic escape, appears to be quite distinct from the X chromosome inactivation process in eutherians. We find no evidence for a polar spread of inactivation from an X inactivation center.This project was funded by grants to JAMG and PDW from the Australian Research Council

Cancer Informatics for Cancer Centers (CI4CC): Building a Community Focused on Sharing Ideas and Best Practices to Improve Cancer Care and Patient Outcomes.

Cancer Informatics for Cancer Centers (CI4CC) is a grassroots, nonprofit 501c3 organization intended to provide a focused national forum for engagement of senior cancer informatics leaders, primarily aimed at academic cancer centers anywhere in the world but with a special emphasis on the 70 National Cancer Institute-funded cancer centers. Although each of the participating cancer centers is structured differently, and leaders' titles vary, we know firsthand there are similarities in both the issues we face and the solutions we achieve. As a consortium, we have initiated a dedicated listserv, an open-initiatives program, and targeted biannual face-to-face meetings. These meetings are a place to review our priorities and initiatives, providing a forum for discussion of the strategic and pragmatic issues we, as informatics leaders, individually face at our respective institutions and cancer centers. Here we provide a brief history of the CI4CC organization and meeting highlights from the latest CI4CC meeting that took place in Napa, California from October 14-16, 2019. The focus of this meeting was "intersections between informatics, data science, and population science." We conclude with a discussion on "hot topics" on the horizon for cancer informatics

Bioactivity of Locally Available Plants on Cotton Whitefly, Bemisia tabaci and the fungus isolated from cotton

Aqueous, diethyl ether, chloroform, petroleum ether, N-hexane and benzene extracts of locally available plant species were tested for phytochemical and insecticidal bioactivity against cotton whitefly, Bemisia tabaci, under controlled conditions. This study is within bioprospection context, for utilizing local plant species as alternative in sustainable agriculture development. The leaf and stem extract was used. The whole plant extract of T.procumbens followed by N.oleander and V.rosea showed repellent and toxic effect against adult and second nymphal instars. Leaf extract of all three plants showed high inhibition activity against nymphal instars. In case of flower extract less inhibition activity was shown respectively. Fungi which grow on the cotton plant was screened, characterized and checked for antifungal activity against the extracts of the plant material. Phytochemical analysis was also carried out by standard protocols

A study of hypoglycemic effect of Caesalpinia bonduc extract on alloxan induced diabetic albino rats

Background: The objective of the study was to scientifically investigate the oral hypoglycemic activity of Caesalpinia bonduc on Alloxan induced diabetic albino rats. To compare the hypoglycemic effect of Caesalpinia bonduc with that of the standard drug Glibenclamide used in the treatment of diabetes mellitus.Methods: Adult healthy albino rats of wister strain of either sex weighing 150-200gms were included in the study. The animals were divided into 4 groups namely control, diabetic control, standard and test groups with 6 animals in each group. Diabetes was chemically induced using alloxan to produce hyperglycemia in rats. Standard drug Glibenclamide suspended in gum acacia was administered for standard group. Test drug Caesalpinia bonduc was administered for test group. Morning around 9 a.m. blood glucose levels were recorded on 1st, 3rd, 7th, 14th, 21st, and 28th days.Results: The control group of rats showed no variation. The diabetic control rats showed consistent hyperglycemia. Comparing the test drug Caesalpinia bonduc to the standard drug Glibenclamide, the test drug was 1.38 times more efficacious than the standard.Conclusions: The alcoholic extract of Caesalpinia bonduc (seeds) has shown more anti diabetic activity by lowering the blood glucose levels in diabetic rats significantly. These findings suggest that hypoglycemic potential of the test compound Caesalpinia bonduc is promising and found to be more significant than the standard compound

Quench Protection System of Superconducting Quadrupole Magnet: Simulation and Experimental Studies

The main motivation behind this thesis is to design an efficient quench protection system (QPS)for the superconducting quadruple magnet system under development at IUAC. A detailed the magneto-static simulations have been done for the quadruple magnet using OPERA-3D software to analyse the field profile and the magnet bore, fringe field and peak field in the conductor of the magnet system. Quench simulation with different QPS circuit configurations using OPERA-QUENCH code for a single NbTi coil and a quadrupole singlet is one of the major objectives of the thesis. One of the configurations of the protection circuit for single NbTi coil has been tested at 4.2K in a test rig. Single superconducting quadrupole coil is simulated in QUENCH package with adiabatic boundary condition for same circuit operated at current of 80A and 100A, it results in linear increment of final maximum temperature and resistance of coil after quenching. Depending on the protection circuit such as only resistor, back-to-back diode and back-to-back diode with resistor across the coil. The final temperature varies in between 38 to 47 K. The purpose of the heater power to initiate the quench in the magnet analysed and experimentally validated from the single coil experiment in LHe cryostat. The effect of dumping resistors of 0.91 and 2.5 ? results into the decrement in original time constant (t_C=1.5 s) to decay the remaining current from 1 s and 0.667 s respectively is characterized. Simulation study is extended to superconducting quadruple singlet with many combinations of circuits and gets an idea about mutual coupling between the coils during quench. A helium cryostat has been designed for testing of the QPS of the superconducting quadrupole doublet structure (QDS). And optimized it mechanically for minimum heat load of 21.4 W

EXPLORING TURBULENT WAKES IN A NON-UNIFORMLY STRATIFIED ENVIRONMENT FOR SUBMARINE DETECTION

This study aims to explore the behavior of turbulent wakes generated by a spherical submerged body propagating with constant speed in a non-uniformly stratified fluid. The investigation is based on a series of high-resolution numerical simulations in which the background stratification is systematically varied. We consider one linear and five non-linear temperature profiles and two sets of Froude numbers (Fr), Fr = 1.0 and Fr = 3.2. The analysis of dissipation of thermal variance (χ) shows that the shape of the wake for non-uniform profiles is more horizontally spread, and internal waves are much stronger than in linear stratification. Experiments with Fr = 1.0 show a rather asymmetric energy distribution caused by internal wave reflections from low-gradient regions. An idealized model demonstrates that internal waves emitted at horizontal angles shallower than roughly 64 degrees are reflected. For Fr = 3.2, internal waves are radiated at steeper angles and transmitted more. Using decay rates of χ, the maximum detection time of the wake can be estimated, showing that for Fr = 3.2, the thermal signal lasts four to five times longer than for Fr = 1.0. Furthermore, concave profiles produce signals lasting approximately twice as long as those for linear profiles, whereas low-gradient types have half the duration. This research is expected to assist in the development of non-traditional detection algorithms for undersea warfare.Office of Naval Research, 875 North Randolph Street, Arlington, VA 22203Outstanding ThesisCapitão de Corveta, Brazilian NavyApproved for public release. Distribution is unlimited