Effect of fuel molecular structure on soot formation in gas turbine combustion

The effect of fuel variations at the same hydrogen content on the formation of soot in a gas turbine combustor was studied. Six fuels were burned to a combustor over a matrix of about 50 test conditions with test conditions ranging over 500-1800 kPa (5-18 atm) pressure and 500-1000 K burner inlet temperature; fuel-air ratios were varied from 0.008-0.024. Flame radiation measurements were made through a sapphire window toward the end of the primary zone. The hydrogen content of the six test fuels ranged from 12.80 to 12.88%. Five fuels emphasized hydrocarbon types: (mono, di, and tricyclic), naphthenes (decalin) and partially hydrogenated aromatics (tetralin); the sixth fuel emphasized final boiling point

Fuel quality combustion analysis

A high pressure research combustor operating over a wide range of burner inlet conditions was used to determine the effects of fuel molecular structure on soot formation. Six test fuels with equal hydrogen content (12.8%) were blended to stress different molecular components and final boiling points. The fuels containing high concentrations (20%) of polycyclic aromatics and partially saturated polycyclic structures such as tetralin, produced more soot than would be expected from a hydrogen content correlation for typical petroleum based fuels. Fuels containing naphthenes such as decalin agreed with the hydrogen content correlation. The contribution of polycyclic aromatics to soot formation was equivalent to a reduction in fuel hydrogen content of about one percent. The fuel sensitivity to soot formation due to the polycyclic aromatic contribution decreased as burner inlet pressure and fuel/air ratio increased

Low-perigee aerodynamic heating during orbital flight of an atmosphere Explorer

An extensive, low-perigee orbital aerodynamic heating study was undertaken in support of the Atmosphere Explorer-C Temperature Alarm. State of the art of low-density, high-speed flows, some models of the earth's atmosphere, external flow-field definition, thermodynamic and transport properties of atmospheric gases, the accommodation coefficient orbital thermal environment, and correlation of theory and measurements are discussed. Aerodynamic heating rates are determined for eight selected orbits by means of a reduced, analytical model verified by both ground test and flight data. These heating rates are compared with classical free-molecule and first-order collision regime values

Convergent transcriptional profiles induced by endogenous estrogen and distinct xenoestrogens in breast cancer cells

Estrogen receptors display high levels of promiscuity in accommodating a wide range of ligand structures, but the functional consequence of changing receptor conformations in complex with distinct agonists is highly controversial. To determine variations in the transactivation capacity induced by different estrogenic agonists, we assessed global transcriptional profiles elicited by natural or synthetic xenoestrogens in comparison with the endogenous hormone 17β-estradiol. Human MCF7 and T47D carcinoma cells, representing the most frequently used model systems for tumorigenic responses in the mammary gland, were synchronized by hormone starvation during 48 h. Subsequently, a 24 h exposure was carried out with equipotent concentrations of the selected xenoestrogens or 17β-estradiol. Analysis of messenger RNA was performed on high-density oligonucleotide microarrays that display the sequences of 33 000 human transcripts, yielding a total of 181 gene products that are regulated upon estrogenic stimulation. Surprisingly, genistein (a phytoestrogen), bisphenol-A and polychlorinated biphenyl congener 54 (two synthetic xenoestrogens) produced highly congruent genomic fingerprints by regulating the same range of human genes. Also, the monotonous genomic signature observed in response to xenoestrogens is identical to the transcriptional effects induced by physiological concentrations of 17β-estradiol. This striking functional convergence indicates that the transcription machinery is largely insensitive to the particular structure of estrogen receptor agonists. The occurrence of such converging transcriptional programs reinforces the hypothesis that multiple xenoestrogenic contaminants, of natural or anthropogenic origin, may act in conjunction with the endogenous hormone to induce additive effects in target tissue

Assessment of slope failures off Vancouver Island revealed in EM300 multibeam bathymetry data

Multibeam bathymetric data acquired off Vancouver Island across the accretionary prism of the Cascadia subduction zone reveal a prominent segmentation of the deformation front with dominant azimuths of the ridges at ~120° and ~150° and abundant submarine landslides. Both these ridge-orientations are oblique to the direction of subduction (~45°). Ridges at a strike of ~120° show dominantly rectangular-shaped failure head-scarps and intact blocks of sediments within the failed sediment mass, whereas ridges with an azimuth of ~150° show curved head-scarps and incoherent debris in the failure mass. We propose that this systematic change in failure-style is related to the underlying thrust fault system producing steeper and taller ridges for azimuths around 150°, but less steep and tall ridges at 120°. Thus, debris-flow style failure is simply a result of higher kinetic forcing of the down-sliding sediment mass: more mixing and destruction of the coherent blocks for taller and steeper ridges, and blocks of intact sediment for gentle slopes and less elevated ridges. A segmentation of the deformation front and ridge alignment into two dominant azimuths could be a result of: a) complex interaction and competing forces from overall slab-pull (45°), b) re-activated faults orientated almost N-S (~175°) on the oceanic plate and overlying sediment cover (reflected in the magnetic stripes and abyssal plain strike-slip faulting), and c) relative orientation of the back-stop off Vancouver Island and accreted terranes (at ~127° following the coastline between Nootka Island and Port Renfrew). Extensional faulting is observed only at ridges with debris-flow style failure, which also are the ridges with larger height and steeper slopes. These extensional faults may be the result of over-steepening of the ridges and collapse of the sediment pile that can no longer withstand its own weight due to limited internal shear strength

Global-genome Nucleotide Excision Repair Controlled by Ubiquitin/Sumo Modifiers

Global-genome nucleotide excision repair (GG-NER) prevents genome instability by excising a wide range of structurally unrelated DNA base adducts and crosslinks induced by chemical carcinogens, ultraviolet (UV) radiation or intracellular metabolic by-products. As a versatile damage sensor, xeroderma pigmentosum group C (XPC) protein initiates this generic defense reaction by locating the damage and recruiting the subunits of a large lesion demarcation complex that, in turn, triggers the excision of aberrant DNA by endonucleases. In the very special case of a DNA repair response to UV radiation, the function of this XPC initiator is tightly controlled by the dual action of cullin-type CRL4DDB2 and sumo-targeted RNF111 ubiquitin ligases. This twofold protein ubiquitination system promotes GG-NER reactions by spatially and temporally regulating the interaction of XPC protein with damaged DNA across the nucleosome landscape of chromatin. In the absence of either CRL4DDB2 or RNF111, the DNA excision repair of UV lesions is inefficient, indicating that these two ubiquitin ligases play a critical role in mitigating the adverse biological effects of UV light in the exposed skin

Dendritic subglacial drainage systems in cold glaciers formed by cut-and-closure processes

The routing and storage of meltwater and the configuration of drainage systems in glaciers exert a profound influence on glacier behaviour. However, little is known about the hydrological systems of cold glaciers, which form a significant proportion of the total glacier population in the climate sensitive region of the High Arctic. Using glacio-speleological techniques, we obtained direct access to explore and survey three conduit systems and one moulin within the tongue area of Tellbreen, a small cold-based valley glacier in central Spitsbergen. More than 600 m of conduits were surveyed and mapped in plan, profile and cross-section view to analyse the configuration of the drainage system. The investigations revealed that cold-based glaciers can exhibit a dendritic drainage network with supra-, en- and subglacial components formed most likely by cut-and-closure processes as well as surface-to-bed drainage via moulins. Furthermore, we observed that water is stored within the glacier and released gradually via subglacial conduits during the winter months, forming a large and active icing in the proglacial area. The presence of supra-, en- and subglacial components, the surface-to-bed moulin and the dendritic subglacial drainage network suggest that existing models and understanding of the hydrology of cold glaciers needs to be re-evaluated, mostly concerning the different possible pathways and processes that form the hydrological system

Catalytic diesel particulate filters reduce the in vitro estrogenic activity of diesel exhaust

An in vitro reporter gene assay based on human breast cancer T47D cells (ER-CALUX®) was applied to examine the ability of diesel exhaust to induce or inhibit estrogen receptor (ER)-mediated gene expression. Exhaust from a heavy-duty diesel engine was either treated by iron- or copper/iron-catalyzed diesel particulate filters (DPFs) or studied as unfiltered exhaust. Collected samples included particle-bound and semivolatile constituents of diesel exhaust. Our findings show that all of the samples contained compounds that were able to induce ER-mediated gene expression as well as compounds that suppressed the activity of the endogenous hormone 17β-estradiol (E2). Estrogenic activity prevailed over antiestrogenic activity. We found an overall ER-mediated activity of 1.63 ± 0.31ng E2 CALUX equivalents (E2-CEQs) per m3 of unfiltered exhaust. In filtered exhaust, we measured 0.74 ± 0.07 (iron-catalyzed DPF) and 0.55 ± 0.09ng E2-CEQ m−3 (copper/iron-catalyzed DPF), corresponding to reductions in estrogenic activity of 55 and 66%, respectively. Our study demonstrates that both catalytic DPFs lowered the ER-mediated endocrine-disrupting potential of diesel exhaus

Short-patch correction of C/C mismatches in human cells

We examined whether the human nucleotide excision repair complex, which is specialized on the removal of bulky DNA adducts, also displays a correcting activity on base mismatches. The cytosine/cytosine (C/C) lesion was used as a model substrate to monitor the correction of base mismatches in human cells. Fibroblasts with different repair capabilities were transfected with shuttle vectors that contain a site-directed C/C mismatch in the replication origin, accompanied by an additional C/C mismatch in one of the flanking sequences that are not essential for replication. Analysis of the vector progeny obtained from these doubly modified substrates revealed that C/C mismatches were eliminated before DNA synthesis not only in the repair-proficient background, but also when the target cells carried a genetic defect in long-patch mismatch repair, in nucleotide excision repair, or when both pathways were deleted. Furthermore, cells deficient for long-patch mismatch repair as well as a cell line that combines mismatch and nucleotide excision repair defects were able to correct multiple C/C mispairs, placed at distances of 21-44 nt, in an independent manner, such that the removal of each lesion led to individual repair patches. These results support the existence of a concurrent short-patch mechanism that rectifies C/C mismatche