Effects of mycophenolate mofetil on cutaneous lupus erythematosus in (NZB × NZW) F1 mice

AbstractBackgroundFew studies have evaluated the effects and precise molecular mechanism of mycophenolate mofetil (MMF) in the treatment of human cutaneous lupus erythematosus (CLE). Our findings shed light on the therapeutic effects of MMF in a UVB-induced NZB × NZW (NZBW) F1 CLE mouse model.MethodsContinuous MMF treatment (60 mg/kg/day) was administered up to Day 50 from the beginning of UVB induction (Day 0; 20 weeks old), as the pathologic features of CLE are present after 50 days. The therapeutic effects of MMF treatment in NZBW lupus mice were examined by comparing histopathological changes, lupus band test (deposition of immune complexes at the dermal–epidermal junction) and colocalization of autoantibodies with a dermal autoantigen Dsg3, and by evaluating the associations of local matrix metalloprotease activities.ResultsMMF improved survival in the NZBW lupus mice from 35.7% to 81.8%. The proteinuria, blood urea nitrogen, and interleukin 6 levels were significantly reduced after MMF treatment. The dermal lymphocytic infiltration, deposition of immune complexes at the dermal–epidermal junction, colocalized autoantibodies with Dsg3, and epidermal matrix metalloprotease activity were also attenuated in MMF-treated NZBW F1 mice.ConclusionThe results confirmed that MMF could substantially attenuate skin damage due to CLE in the NZBW F1 mouse model

Structural and cognitive deficits in chronic carbon monoxide intoxication: a voxel-based morphometry study

BACKGROUND: Patients with carbon monoxide (CO) intoxication may develop ongoing neurological and psychiatric symptoms that ebb and flow, a condition often called delayed encephalopathy (DE). The association between morphologic changes in the brain and neuropsychological deficits in DE is poorly understood. METHODS: Magnetic resonance imaging and neuropsychological tests were conducted on 11 CO patients with DE, 11 patients without DE, and 15 age-, sex-, and education-matched healthy subjects. Differences in gray matter volume (GMV) between the subgroups were assessed and further correlated with diminished cognitive functioning. RESULTS: As a group, the patients had lower regional GMV compared to controls in the following regions: basal ganglia, left claustrum, right amygdala, left hippocampus, parietal lobes, and left frontal lobe. The reduced GMV in the bilateral basal ganglia, left post-central gyrus, and left hippocampus correlated with decreased perceptual organization and processing speed function. Those CO patients characterized by DE patients had a lower GMV in the left anterior cingulate and right amygdala, as well as lower levels of cognitive function, than the non-DE patients. CONCLUSIONS: Patients with CO intoxication in the chronic stage showed a worse cognitive and morphologic outcome, especially those with DE. This study provides additional evidence of gray matter structural abnormalities in the pathophysiology of DE in chronic CO intoxicated patients

Structural insights into the electron/proton transfer pathways in the quinol:fumarate reductase from Desulfovibrio gigas

The membrane-embedded quinol:fumarate reductase (QFR) in anaerobic bacteria catalyzes the reduction of fumarate to succinate by quinol in the anaerobic respiratory chain. The electron/proton-transfer pathways in QFRs remain controversial. Here we report the crystal structure of QFR from the anaerobic sulphate-reducing bacterium Desulfovibrio gigas (D. gigas) at 3.6 Å resolution. The structure of the D. gigas QFR is a homo-dimer, each protomer comprising two hydrophilic subunits, A and B, and one transmembrane subunit C, together with six redox cofactors including two b-hemes. One menaquinone molecule is bound near heme b_L in the hydrophobic subunit C. This location of the menaquinone-binding site differs from the menaquinol-binding cavity proposed previously for QFR from Wolinella succinogenes. The observed bound menaquinone might serve as an additional redox cofactor to mediate the proton-coupled electron transport across the membrane. Armed with these structural insights, we propose electron/proton-transfer pathways in the quinol reduction of fumarate to succinate in the D. gigas QFR

Experimental ten-photon entanglement

Quantum entanglement among multiple spatially separated particles is of fundamental interest, and can serve as central resources for studies in quantum nonlocality, quantum-to-classical transition, quantum error correction, and quantum simulation. The ability of generating an increasing number of entangled particles is an important benchmark for quantum information processing. The largest entangled states were previously created with fourteen trapped ions, eight photons, and five superconducting qubits. Here, based on spontaneous parametric down-converted two-photon entanglement source with simultaneously a high brightness of ~12 MHz/W, a collection efficiency of ~70% and an indistinguishability of ~91% between independent photons, we demonstrate, for the first time, genuine and distillable entanglement of ten single photons under different pump power. Our work creates a state-of-the-art platform for multi-photon experiments, and provide enabling technologies for challenging optical quantum information tasks such as high-efficiency scattershot boson sampling with many photons.Comment: 65 pages, supplementary information included, with all raw data. to appear in Physical Review Letter

Revisit of tensor-meson nonet in resonance chiral theory

We study the properties of the lowest multiplet of light-flavor tensor meson resonances, i.e. $f_2(1270)$, $a_2(1320)$, $K_2^*(1430)$, and $f_2'(1525)$, within the resonance chiral theory approach. The higher-order resonance chiral operators, including the light-quark mass and $1/N_C$ corrections, are simultaneously incorporated in our study. The use of resonance chiral expressions allows us to analyze not only the relevant experimental data but also in the meantime the lattice results at unphysical quark masses, including the masses of the lowest multiplet of tensor resonances and their decay widths into two pseudoscalar mesons. In addition, the radiative decays of the tensor resonances into one photon plus one pseudoscalar meson and two photons are also studied.Comment: 18 pages, 3 tables, 3 figures. To match the published versio