Real Time- Polymerase Chain Reaction (RT-PCR) Test to Diagnose Brucellosis in Sheep in ANBAR Province

The aims of the study were to determine incidence ratio of brucellosis in aborted ewes in Anbar governorate using real time PCR technique and bacterial culture and evaluating the efficiency of bacterial culture. 50 samples of stomach contents of an aborted fetus and vaginal swabs from aborted ewes were collected. The incidence ratio of brucellosis in ewes in Anbar governorate were 72% in PCR test However in bacterial culture were 46%. The positive prediction value and Negative prediction value and Sensitivity and Specificity of bacterial culture were (100%, 51.8 %,63%,100%) respectively when use Real time PCR as an assessment test. Conclusions: Anbar province is endemic with brucellosis, especially in sheep. The Real Time PCR is a fast, easy and accurate test in detecting even small numbers of bacteria in a sample, Significant superiority of polymerase chain reaction technology over bacterial culture

The response of the maize nitrate transport system to nitrogen demand and supply across the lifecycle

The definitive version is available at www.newphytologist.comAn understanding of nitrate (NO3-) uptake throughout the lifecycle of plants, and how this process responds to nitrogen (N) availability, is an important step towards the development of plants with improved nitrogen use efficiency (NUE). NO3- uptake capacity and transcript levels of putative high- and low-affinity NO3- transporters (NRTs) were profiled across the lifecycle of dwarf maize (Zea mays) plants grown at reduced and adequate NO3-. Plants showed major changes in high-affinity NO3- uptake capacity across the lifecycle, which varied with changing relative growth rates of roots and shoots. Transcript abundances of putative high-affinity NRTs (predominantly ZmNRT2.1 and ZmNRT2.2) were correlated with two distinct peaks in high-affinity root NO3- uptake capacity and also N availability. The reduction in NO3- supply during the lifecycle led to a dramatic increase in NO3- uptake capacity, which preceded changes in transcript levels of NRTs, suggesting a model with short-term post-translational regulation and longer term transcriptional regulation of NO3- uptake capacity. These observations offer new insight into the control of NO3- uptake by both plant developmental processes and N availability, and identify key control points that may be targeted by future plant improvement programmes to enhance N uptake relative to availability and/or demand.Trevor Garnett, Vanessa Conn, Darren Plett, Simon Conn, Juergen Zanghellini, Nenah Mackenzie, Akiko Enju, Karen Francis, Luke Holtham, Ute Roessner, Berin Boughton, Antony Bacic, Neil Shirley, Antoni Rafalski, Kanwarpal Dhugga, Mark Tester, and Brent N. Kaise

Population, Land Use and Deforestation in the Pan Amazon Basin: a Comparison of Brazil, Bolivia, Colombia, Ecuador, Perú and Venezuela

This paper discusses the linkages between population change, land use, and deforestation in the Amazon regions of Brazil, Bolivia, Colombia, Ecuador, Perú, and Venezuela. We begin with a brief discussion of theories of population–environment linkages, and then focus on the case of deforestation in the PanAmazon. The core of the paper reviews available data on deforestation, population growth, migration and land use in order to see how well land cover change reflects demographic and agricultural change. The data indicate that population dynamics and net migration exhibit to deforestation in some states of the basin but not others. We then discuss other explanatory factors for deforestation, and find a close correspondence between land use and deforestation, which suggests that land use is loosely tied to demographic dynamics and mediates the influence of population on deforestation. We also consider national political economic contexts of Amazon change in the six countries, and find contrasting contexts, which also helps to explain the limited demographic-deforestation correspondence. The paper closes by noting general conclusions based on the data, topics in need of further research and recent policy proposals.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/42720/1/10668_2003_Article_6977.pd

Genome-wide identification, classification and transcriptional analysis of nitrate and ammonium transporters in Coffea

Abstract Nitrogen (N) is quantitatively the main nutrient required by coffee plants, with acquisition mainly by the roots and mostly exported to coffee beans. Nitrate (NO3–) and ammonium (NH4+) are the most important inorganic sources for N uptake. Several N transporters encoded by different gene families mediate the uptake of these compounds. They have an important role in source preference for N uptake in the root system. In this study, we performed a genome-wide analysis, including in silico expression and phylogenetic analyses of AMT1, AMT2, NRT1/PTR, and NRT2 transporters in the recently sequenced Coffea canephora genome. We analyzed the expression of six selected transporters in Coffea arabica roots submitted to N deficiency. N source preference was also analyzed in C. arabica using isotopes. C. canephora N transporters follow the patterns observed for most eudicots, where each member of the AMT and NRT families has a particular role in N mobilization, and where some of these are modulated by N deficiency. Despite the prevalence of putative nitrate transporters in the Coffea genome, ammonium was the preferential inorganic N source for N-starved C. arabica roots. This data provides an important basis for fundamental and applied studies to depict molecular mechanisms involved in N uptake in coffee trees

Nitrogen acquisition by roots: physiological and developmental mechanisms ensuring plant adaptation to a fluctuating resource

International audienceNitrogen (N) is one of the key mineral nutrients for plants and its availability has a major impact on their growth and development. Most often N resources are limiting and plants have evolved various strategies to modulate their root uptake capacity to compensate for both spatial and temporal changes in N availability in soil. The main N sources for terrestrial plants in soils of temperate regions are in decreasing order of abundance, nitrate, ammonium and amino acids. N uptake systems combine, for these different N forms, high- and low-affinity transporters belonging to multige families. Expression and activity of most uptake systems are regulated locally by the concentration of their substrate, and by a systemic feedback control exerted by whole-plant signals of N status, giving rise to a complex combinatory network. Besides modulation of the capacity of transport systems, plants are also able to modulate their growth and development to maintain N homeostasis. In particular, root system architecture is highly plastic and its changes can greatly impact N acquisition from soil. In this review, we aim at detailing recent advances in the identification of molecular mechanisms responsible for physiological and developmental responses of root N acquisition to changes in N availability. These mechanisms are now unravelled at an increasing rate, especially in the model plant Arabidopsis thaliana L.. Within the past decade, most root membrane transport proteins that determine N acquisition have been identified. More recently, molecular regulators in nitrate or ammonium sensing and signalling have been isolated, revealing common regulatory genes for transport system and root development, as well as a strong connection between N and hormone signalling pathways. Deciphering the complexity of the regulatory networks that control N uptake, metabolism and plant development will help understanding adaptation of plants to sub-optimal N availability and fluctuating environments. It will also provide solutions for addressing the major issues of pollution and economical costs related to N fertilizer use that threaten agricultural and ecological sustainability

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HR Activities of Tory Fashion Wear Ltd

I am a student of BBA program so an internship is a mandatory part of my university journey. I have completed my internship program from respected garments and the name of the manufacturing garments is “Tory Fashion Wear Ltd”. I have a great experience in this sector. I have worked there for 3 months and this three months I have not only worked there but also experienced it. In this sector, I have gained valuable knowledge and experience in Human Resource Management as my major was HRM. I have completed this report based on my experience and gather knowledge. This report is all about HR activities that I have educated in these three months. In this report, I have to pay attention to all the core activities of the Human Resource Department. How they manage the department, what are the core values of their department, how they provide their services to the employees these all are findings I have to originate and worked on it. In this report I have also described in detail what works I have done there like, CV screening, data entry, making report and policy, absenteeism list, job posting, assist to take interviews, help out to giving training, make an effort to motivate employees to do their job properly. Lend a hand to the development program teaches precise administration and organization technique. I originate in Tory Fashion Wear Ltd they were strictly following what their HR department notified to them and also very confidential especially focus on employees and their rights. They are very concern about their employee and as well as labor cause they are the actual asset for a garments industry. As it is a garment they have to focus on both sides employees of the office and on the other hand labor. When training arrange for both have to make plans. I have seen their facilities and other benefits and I tried to spotlight on that and also their evaluation process. I have also given some recommendation based on my findings and at the end of my report, I have attached the surveys and questionnaire