Genistein-induced proteome changes in the human endometrial carcinoma cell line, ishikawa

Epidemiological studies have shown that Asian populations display a lower incidence of hormone-dependant cancers, cardiovascular disease, osteoporosis, and menopausal ailments compared to Western societies. Available data support the proposal that lower incidence is associated with the high dietary consumption of isoflavones, such as genistein. This study used two-dimensional electrophoresis to characterize the effect of genistein on the proteome of an endometrial tumor cell model, namely the Ishikawa cell line. Proteome maps displaying approx 1800 proteins were obtained from cells treated with vehicle or genistein at physiologically attainable concentrations of 0.5, 5, or 50 μM or supra-physiological concentration, 500 μM. The effects of genistein on protein expression were characterized using image analysis software. A total 65 protein spots displayed a significant decrease in expression and 32 proteins displayed a significant increase in expression. Of these protein spots, 29 were randomly selected for characterization by matrix assisted laser desorption/ionization tandem mass spectrometry, yielding 18 different proteins. This type of analysis enabled the characterization of a wide range of cellular proteins and allowed for the identification of functional and biochemical pathways that may be regulated or affected by genistein, including cellular transcription, cell proliferation, stress response, or modulation of oncogenic pathways.15 page(s

Decolonising the 'red' reflex test : transitioning from terminology based on colour to anatomy

Peer reviewe

Maternal Prenatal Depression and Anxiety Affect Neonatal Neurobehavior

Prenatal depression and anxiety have been linked to impaired infant neurobehavior including altered attention, responsivity, and quality of movement. No known studies have assessed individual and joint effects of prenatal depressive and anxiety on neonatal neurobehavioral outcomes. The current study compared these outcomes among (1) mothers diagnosed with comorbid Depressive and Anxiety Disorders, (2) mothers diagnosed with only Depressive Disorders, (3) mothers diagnosed with only Anxiety Disorders, and (4) mothers with no prenatal diagnosis. Maternal prenatal diagnoses were assessed by the SCID-I. Offspring neurobehavior was assessed from 1-2 to 30 days using the NNNS. There was a between-subject\u27s effect (n=248) of maternal diagnostic group on infants\u27 self-regulation, quality of movement, and hypertonicity from birth to 30 days. Mothers with no diagnosis had infants with better self-regulation than those with depression or anxiety, better quality of movement than those with depression, and were less likely to have hypertonic infants than those with individual or comorbid depression. Maternal diagnoses may differentially affect offspring neurobehavioral profiles

Das planetarische politisch(e) Denken

No abstract

Creating testable questions in practical conservation:a process and 100 questions

It is now clear that the routine embedding of experiments into conservation practice is essential for creating reasonably comprehensive evidence of the effectiveness of actions. However, an important barrier is the stage of identifying testable questions that are both useful but also realistic to carry out without a major research project. We identified approaches for generating such suitable questions. A team of 24 participants crowdsourced suggestions, resulting in a list of a hundred possible tests of actions.</p

Wheaton Quarterly

Fall 2016 issue of the Wheaton QuarterlyBetween the Lines: I think, therefore I am, right?, pg.2@PRESIDENTHANNO: Sparking possibilities, pg.3Around the Dimple: Lead the way, pg.4Around the Dimple: Graduates heading off into the world of work, pg.6Around the Dimple: Fulbright wins, pg.8Around the Dimple: The code of learning, pg.9Around the Dimple: Story to screen, pg.10Around the Dimple: Tribeca Film Festival screens documentary student had hand in editing, pg.11Around the Dimple: Digital humanities leads to Beinecke award, pg.12Around the Dimple: First Wheaton freshman wins Projects for Peace grant, pg.12Around the Dimple: Teaching the power of presence, pg.13Around the Dimple: Wheaton appoints new provost, pg.14Around the Dimple: Two professors win Whiting fellowships, pg.15Panorama: Cancer through a historical lens, pg.16Panorama: Publications, Honors and Creative Works, pg.17Field Report: Playing it forward, pg.18Field Report: On the right track, pg.19Snap Shots: Talent show, pg.20Courage, compassion and forgiveness, pg.22What can you do with a philosophy degree?, pg.26Alumnae/i Association News: Backstage in spotlight, pg.32Alumnae/i Association News: Welcome new board members, pg.33Alumnae/i Association News: Reunion 2016, pg.34Class Notes: Getting down to business, pg.36Class Notes: Mesmerizing tales, pg.37Class Notes: Empowered woman, pg.38Class Notes: Returning to campus to offer opportunities, pg.39Class Notes: Stay connected, pg.40Class Notes: Brick by brick, pg.41Class Notes (removed), pg.42In Memoriam, pg.62End Page: Commencement Reunion by the numbers, pg.6

Creating testable questions in practical conservation: a process and 100 questions

It is now clear that the routine embedding of experiments into conservation practice is essential for creating reasonably comprehensive evidence of the effectiveness of actions. However, an important barrier is the stage of identifying testable questions that are both useful but also realistic to carry out without a major research project. We identified approaches for generating such suitable questions. A team of 24 participants crowdsourced suggestions, resulting in a list of a hundred possible tests of actions