Influence of propolis biologically active substances on blood biochemical parameters and morphometric indicators of intestines of store pigs

The results of research in some cases allow to judge the change in the functional activity of the studied organs after the use of the preparation. The obtained factual material significantly supplements the information about the biological properties of the propolis water-alcohol emulsion. In practical veterinary medicine, the results of research can be taken into account when prescribing propolis for therapeutic and preventive purposes. Thus, as a result of the study, it was found that the most characteristic histological changes in the structural components of the intestinal wall are observed in the ileum of young pigs and pigs of the experimental groups. The height of this intestine villi in young pigs of the experimental group is 280.97 ± 3.37 microns, and in the control group - 193.89 ± 2.66 microns (p&lt; 0.001). In pigs treated with the preparation, the villi also have a higher height - 256.89 ± 1.62 microns compared to 163.96 ± 0.61 microns in the control group. In young pigs and pigs of the control groups, the shape of the ileum villi is finger-shaped or bottle-shaped, and in animals of the experimental groups their forms are more differentiated - bottle-shaped, leaf-shaped and dome-shaped. This will allow store pigs receiving propolis preparation to better absorb the nutrients of the diet and gain weight faster. The research results are of interest to researchers developing propolis preparations for oral use.</jats:p

DYNAMICS OF INSULIN-LIKE GROWTH FACTOR-1 (IGF-1) IN CHILDREN AFTER AB0-INCOMPATIBLE LIVER TRANSPLANTATION

It is shown that liver transplantation (LT) from donor with incompatible blood type (AB0i) may be effective and safe, but the impact of such operation upon the various systems of the body has not been investigated yet. Insulinlike growth factor-1 (IGF-1) is synthesized in the liver and mediates the action of growth hormone. The level of IGF-1 is a marker of the processes of cell proliferation and tissue regeneration. Aim. To evaluate levels of IGF-1 in children-recipients with liver transplant from AB0i (incompatible) and AB0c (compatible) donors.Materials and methods. 140 children aged 3 to 36 (19,5 ± 16,5 months) with congenital diseases of the hepatobiliar system, 58 of them boys, were surveyed. All patients underwent transplantation of left lateral liver sector from living related donors: 111 children were transplanted with fragment of the liver from AB0c donors, 29 – from AB0i donors; in 10 children with AB0i liver before and/or after LT operation anti-group antibodies (anti-A/B) were revealed. The concentration of IGF-1 was determined by ELISA using specifi c kits (Immunodiagnostic System, USA) in samples of blood plasma, which were received up to a month and a year after a liver transplant.Results. Average level of IGF-1 21,0 ± 29,5 μg/l in patients before LT was signifi cantly lower than in healthy children (52,2 ± 26,3 μg/l, p &lt; 0,001) and did not vary in children, having received later a piece of liver from a compatible (AB0c) donor and from donor AB0i (23,5 ± 30,9 and 21,2 ± 23,2 μg/l respectively, p = 0,70). In patients with anti-A/B prior to surgery average level of IGF-1 was not different from that of the patients without antibodies (32,6 ± 27,6 and 22,3 ± 29,6 μg/l respectively, p = 0,4). One month after LT level of IGF-1 has increased both in the general group, and in patients with AB0c and AV0i liver (92,1 ± 77,8 and 131,2 ± 106,7 μg/l respectively, p = 0,09). The level of IGF-1 was not varied in the group with antibodies (152,5 + 150,4 μg/l) and without them (95,9 ± 77,0 μg/l). A year after LT the average level of IGF-1 in recipients of AV0c and AV0i liver was not varied and was signifi cantly higher than before LT (82,0 + 60,7 and 91,2 ± 77,8 μg/l, p &lt; 0,005 and p = 0,03 respectively). The content of IGF-1 in patients with anti-A/B and without them (104,7 ± 67,5 and 84,7 ± 63,7 μg/l respectively) also did not differ.Conclusion: the results of our studies have shown that restoration of the level of IGF-1 is not dependent on transplantation of compatible or incompatible blood type liver, as well as on the availability of anti-group antibodies

Immunological markers in the diagnosis of inflammatory bowel diseases in children

Objective. To study the specificities of autoimmune response in children suffering from Crohn's disease and interrelationships between autoagression parameters. Patients and methods. We examined 62 patients aged 2–17 years with a confirmed diagnosis of Crohn’s disease. The examination included flow cytometric immunophenotyping of lymphocytes, examination of serum concentrations of total and specific immunoglobulins IgE against allergens of the fungi Saccharomyces cerevisiae and Candida albicans (enzyme-linked immunosorbent assay – ELISA), circulating immune complexes (CICs), concentrations of autoantibodies (AB) against S. cerevisiae (ASCA) IgA and IgG, anti-neutrophil cytoplasmic AB (ANCA MPO, ANCA PR3), antinuclear AB (ANAs), AB against DNAds, DNAss, autoantibodies against antigens of the small and large intestines, and the pancreas (ELISA). Results. Activation of helper T cells, decreased NK cell levels, higher levels of γδ-Т cells were detected. Specific IgЕ against к S. cerevisiae could be noted in 70.9% of patients. In 35.5% of cases, higher levels of ASCA (IgA, IgG) were found. Blood concentrations of AB against DNAds, DNAss exceeded the norm in 4.8 and 16.1% of patients, respectively. Higher levels of CICs were found in 20 (32.3%) of patients. ANAs concentrations corresponded to the norm. Strong positive correlations between many parameters of autoimmune response are indicative of the intensity of the immunopathological process in CD. Conclusion. Immunological diagnosis in CD is necessary for assessing the severity of disease, for optimization of differential diagnosis, establishment of a prognosis and selection of individual therapy for regulation of immune responses. Key words: autoantibodies, Crohn’s disease, immunodiagnosis, immunophenotyping of lymphocytes</jats:p

META-TECHNOLOGIES OF BUSINESS AND PROFESSIONAL CONSULTING IN EDUCATIONAL ORGANIZATIONS

The article is devoted to the analysis of the main principles of business and professional consulting as a practice of interpersonal relations and the being of its subjects. The main problems of clients connected with violations of their intrapersonal and interpersonal relations, as well as relations to their own life (being) in general, subjects of business consulting as participants in business processes and life, in general, are considered. The purpose of the study is to analyze the basic principles or meta-technologies of business and professional consulting in educational organizations. The article summarizes the basic concepts and concepts of business and professional consulting, reflecting its meaning as helping the socio-psychological practice of developing people and educational organizations. Relying on the tetrad of principles of psychological counselling, isolated in the school of C.R. Rogers, as well as the leading principles of organizational counselling in educational organizations, the authors consider their manifestations and features in the main aspects and dimensions of advisory interaction. Traditionally, such dimensions are distinguished: power/management system, competence/experience system, love/system of relations. Each of these dimensions is associated with one or another intention of consultative relations in educational organizations: transformational, dialogical, and relational. Within each of these intentions, the basic principles of business and professional consulting in educational organizations are manifested as meta-technologies or meta-technology groups. In addition, the authors consider the principles of business and professional consulting in educational organizations in relation to the life of the consultant and the client as a whole, as "meta-technologies of life".</jats:p

PECULIARITIES OF SARS-COV-2 NUCLEOCAPSID IN CHILDREN DURING THE COVID-19 EPIDEMIC OF 2020

The aim of the study is to summarize the results of a study of seroprevalence of children to SARS-CoV-3, obtained during the implementation of the Rospotrebnadzor program to assess seroprevalence to the NC antigen SARS-CoV-2 of the population of 26 regions of the Russian Federation, conducted during the COVID-19 epidemic in 2020. Research methods: the study of seroprevalence in 26 model territories of the Russian Federation was carried out according to a unified methodology developed by Rospotrebnadzor with the participation of the St. Petersburg Pasteur Institute of Epidemiology and Microbiology. The method provided for the formation of a group of volunteers in a model federative entity, including children aged 1–17 years, in which the presence of antibodies to the SARS-CoV-2 nucleocapsid was determined in venous blood plasma by the enzyme-linked immunosorbent assay (ELISA). The examination of volunteers was carried out in 3 stages: June-August, September-October, December. Results: the study found that in most of the areas surveyed, children had a higher level of seroprevalence than adults. It is shown that high seroprevalence is accompanied by statistically significant decrease in morbidity (p0.05). The analysis of seroprevalence at all three stages revealed at least 3 variants of its dynamics: 1) continuous growth of seroprevalence in parallel with the development of the epidemic process; 2) an episodic change in the periods of increase and decrease in the level of seroprevalence; 3) a continuous decrease in seroprevalence at the level of the epidemic process. Comparative determination of seroconversion levels in three age groups – 1–6, 7–13 and 14–17 years – revealed no statistically significant differences between these age groups have been identified. Conclusion: children aged 1–17 years in most of the surveyed territories of the Russian Federation have a higher seroprevalence to SARS-CoV2 than adults. The increase in seroprevalence in children is accompanied by a decrease in the incidence of COVID-19.</jats:p