1,026 research outputs found

    The anti-adhesive and anti-aggregatory effects of phenolics from Trifolium species in vitro

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    Abstract The present in vitro study includes a compar-ative evaluation of anti-platelet (anti-thrombotic) proper-ties of plant phenolics, isolated from nine different clover (Trifolium) species. The analysis covered phenolic frac-tions isolated from T. alexandrinum L., T. fragiferum L., T. hybridum L., T. incarnatum L., T. pallidum Waldst et Kit., T. resupinatum L. var. majus Boiss, T. resupinatum L. var. resupinatum, T. scabrum L., and T. pratense L. (red clo-ver). The inhibitory effects of plant preparations (1–50 lg/ ml) on hemostatic functions of blood platelets were assessed by measurements of thrombin- or ADP-induced platelet adhesion to fibrinogen, platelet aggregation in platelet-rich plasma (activated with ADP or collagen), and by the determination of PF-4 secretion from platelet a-granules. The influence of T. phenolics on arachidonic cascade in blood platelets was also determined. T. resupinatum var. majus, T. resupinatum var. resupinatum, and T. scabrum had the strongest anti-platelet effects. These preparations displayed the most evident anti-adhe-sive and anti-aggregatory effects in response to all of the used agonists: thrombin (0.2 U/ml), ADP (10 lM), and collagen (2 lg/ml), and their inhibitory properties were also confirmed by an analysis of PF-4 secretion. T. scabrum and some of other examined clover species possess sig-nificantly higher concentrations of both isoflavones and other bioactive phenolics, when compared to red clover. The obtained results suggest that these clovers contain substances with potent anti-platelet properties

    Separation of Oligosaccharides from Lotus Seeds via Medium-pressure Liquid Chromatography Coupled with ELSD and DAD

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    peer-reviewedLotus seeds were identified by the Ministry of Public Health of China as both food and medicine. One general function of lotus seeds is to improve intestinal health. However, to date, studies evaluating the relationship between bioactive compounds in lotus seeds and the physiological activity of the intestine are limited. In the present study, by using medium pressure liquid chromatography coupled with evaporative light-scattering detector and diode-array detector, five oligosaccharides were isolated and their structures were further characterized by electrospray ionization-mass spectrometry and gas chromatography-mass spectrometry. In vitro testing determined that LOS3-1 and LOS4 elicited relatively good proliferative effects on Lactobacillus delbrueckii subsp. bulgaricus. These results indicated a structure-function relationship between the physiological activity of oligosaccharides in lotus seeds and the number of probiotics applied, thus providing room for improvement of this particular feature. Intestinal probiotics may potentially become a new effective drug target for the regulation of immunity

    Vibration reduction of cable-stayed bridge model retrofitted with SMA restrainers

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    Shape Memory Alloys (SMAs) are more and more frequently used in civil structures subjected to extreme dynamic loadings. Due to their unique properties, the SMAs are used for seismic retrofit of bridges, tall buildings and masts. The paper presents selected results of numerical calculations of a cable-stayed steel bridge model retrofitted with SMA restrainers. The original set of constitutive relationships describing pseudoelastic properties of SMA was used for this purpose. The formulation of SMA model is based on analysis of a special rheological scheme representing its constitutive properties. The SMA model was implemented within FEM software Abaqus using an user defined subroutine. It was proved that the application of SMA restrainers can reduce significantly vibrations and internal forces caused by seismic excitations

    Underinsurance in cyclone and flood environments: a case study in Cairns, Queensland

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    This article examines findings from a 2018 project in Cairns, Far North Queensland, on insurance and risk perception. Using a mixed-methods approach, the study explored how people regard insurance, if at all, and how they use it to reduce their risk of financial loss following a disaster event. Results suggest that while most home owners hold insurance, renters are less likely to have contents insurance leaving them financially vulnerable if losing their property. Cost of insurance is considered one barrier to taking out insurance, however, other issues such as risk perception, self-efficacy and trust in insurers are issues for both home owners and renters. The paper furthers knowledge of underinsurance in disaster-prone areas and provides vital information for understanding motivation to withdraw from insurance

    Effective Separation and Recovery of Manganese and Potassium from Biomass Ash by Solvent Extraction

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    Manganese (Mn) is considered an important, energy-critical metal due to its leading role in the production of electrochemical energy storage devices. One valuable source of Mn is hyperaccumulator plants used for the phytoremediation of contaminated soil. In this study, stems and leaves of ginger (Zingiber officinale), which accumulate Mn at moderate levels (∼0.2 wt %) and potassium (K) at high levels (>5 wt %), were analyzed to assess the potential of recovering metals from this plant. The extraction behaviors of Mn and K were studied using raw and ash samples (100−600 °C). It was crucial to set an appropriate incineration temperature (300 °C) to selectively extract K (∼96%) and Mn (∼90%) using water and nitric acid over two consecutive steps. Additionally, citric acid, a cost-effective and environmentally friendly solvent, was just as effective (∼85%) as nitric acid in extracting Mn. X-ray absorbance nearedge spectroscopy and X-ray diffraction analysis of the ash before and after extractions were applied to elucidate the extraction mechanism. The results revealed that selective extraction of both compounds was possible due to the change in the oxidative state of Mn(II) (soluble in water) into Mn(III) and Mn(IV) (insoluble in water) during sample incineration. Simultaneously, there were complex reactions associated with the changes within potassium carbonate compounds; however, these did not affect the K extraction efficiency
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