IR and RI: 外部システムからのスムーズな利用

DRF International Conference 2008 Open Access and Institutional Repository in Asia-Pacific (DRFIC 2008), 30th and 31st January, Osaka, JAPAN / デジタルリポジトリ連合国際会議2008 アジア・環太平洋地域におけるオープンアクセスと機関リポジトリ(DRFIC 2008), 平成20年1月30‐31日, 大阪大学 Poster Session No.5-poster / ポスターセッション No.5-ポスタ

Murine liver allograft transplantation: Tolerance and donor cell chimerism

Nonarterialized orthotopic liver transplantation with no immunosuppression was performed in 13 mouse‐strain combinations. Two strain combinations with major histocompatibility complex class I and class II and minor histocompatibility complex disparity had 20% and 33% survival of more than 100 days, but the other 11 combinations, including four that were fully allogeneic and all with only class I, class II or minor disparities, yielded 45% to 100% survival of more than 100 days. Long‐living recipients permanently accepted donor‐strain heterotopic hearts transplanted on the same day or donor‐strain skin 3 mo after liver transplantation, in spite of detectable antidonor in vitro activity with mixed lymphocyte reaction and cellmediated lymphocytotoxicity testing (split tolerance). In further donor‐specific experiments, liver grafts were not rejected by presensitized major histocompatibility complex class I‐disparate recipients and they protected donor‐strain skin grafts from second set (or any) rejection. Less frequently, liver transplantation rescued rejecting skin grafts placed 1 wk earlier in major histocompatibility complex class I, class II and minor histocompatibility complex, class II or minor histocompatibility complex‐disparate strain combinations. Donor‐derived leukocyte migration to the central lymphoid organs occurred within 1 to 2 hr after liver transplantation in all animals examined, persisted in the surviving animals until they were killed (>375 days), and was demonstrated with double‐immunolabeling to be multilineage. The relation of these findings to so‐called hepatic tolerogenicity and to tolerance in general is discussed. (HEPATOLOGY 1994;19:916–924.) Copyright © 1994 American Association for the Study of Liver Disease

EFEITO DO ESCORE DE CONDIÇÃO CORPORAL(ECC) SOBRE PARÂMETROS REPRODUTIVOS DE VACAS (Bos indicus) SUBMETIDAS À INSEMINAÇÂO ARTIFICIAL EM TEMPO FIXO (IATF)

This study evaluated the effect of BCS on reproductive parameters of Bos indicus cows submitted to IATF. Assessment data from farms in the states of Mato Grosso, São Paulo and Minas Gerais was performed. The animals were analyzed for BCS (scale 1-5) and correlated with the data of follicular dynamics and fertility obtained by synchronization protocol for TAI. Sonographic examinations were performed every 12 hours from implant removal to ovulation for evaluation of follicular dynamics. To fertility, sonographic examinations were performed on the withdrawal of the intravaginal device (D8) at the time of TAI (D10) and diagnosis of pregnancy (D40). Further, the rate of estrus was estimated to be considered when the animals showed a marked on the base of the tail at the moment of AI. It was observed that the diameter of the follicle during the removal of the device and the maximum diameter of the dominant follicle was lower in BCS 2.5 (7.4 ± 0.4, 9.7 ± 0.8, respectively) than in cows BCS 3.0 (9.4 ± 0.3, 12.7 ± 0.5, respectively). In addition, the ovulation rate was lower in the group of cows with low score (BCS = 2.5). To evaluate fertility, animals with BCS 2.5 had lower occurrence of estrus (47.6 %) and lower pregnancy rate (27.1 %) than cows with score 3 (62.1 % and 39.4 %) and 3.5 (67.5% and 45.3 %). We conclude that low ECC can affect reproductive parameters of Bos indicus compromising the reproductive efficiency of these animals.Objetivou-se com este estudo avaliar o efeito do ECC sobre parâmetros reprodutivos de vacas Bos indicus submetidas à IATF. Foi realizada a avaliação de dados de fazendas localizadas nos estados de Mato Grosso, São Paulo e Minas Gerais. Os animais foram analisados quanto ao ECC (escala 1-5) e correlacionados com os dados de dinâmica folicular e fertilidade obtidos pelo do protocolo de sincronização da IATF. Exames ultrassonográficos foram realizados a cada 12 horas a partir da retirada do implante até a ovulação para avaliação da dinâmica folicular. Para avaliação da fertilidade, exames ultrassonográficos foram realizados no dia da retirada do dispositivo intravaginal (D8), no momento da IATF (D10) e diagnóstico de gestação (D40). A taxa de estro foi estimada sendo a mesma considerada quando os animais não apresentavam a base da cauda marcada no momento da IA. Observou-se que o diâmetro do folículo na retirada do dispositivo e o diâmetro máximo do folículo dominante foram menores nas vacas com ECC 2,5 (7,4±0,4; 9,7±0,8, respectivamente) do que nas vacas de ECC 3,0 (9,4±0,3; 12,7±0,5, respectivamente). Além disso, a taxa de ovulação foi menor no grupo de vacas com baixo escore (ECC=2,5). Na avaliação da fertilidade, animais de ECC 2,5 apresentaram menor taxa de cio (47,6%) e menor taxa de prenhez (27,1%) do que vacas com de escore 3 (62,1% e 39,4%) e 3,5 (67,5% e 45,3%). Conclui-se que o baixo ECC é capaz de afetar parâmetros reprodutivos de vacas Bos indicus comprometendo a eficiência reprodutiva desses animais

IR and RI : Smooth Use from External Systems

DRFIC2008 Poster Session No.5DRFIC2008 ポスターセッション資料 5

EGFR and MET receptor tyrosine kinase-altered microRNA expression induces tumorigenesis and gefitinib resistance in lung cancers

The involvement of the MET oncogene in de novo and acquired resistance of non-small cell lung cancers (NSCLC) to tyrosine kinase inhibitors (TKIs) has been reported, but the precise mechanism by which MET overexpression contributes to TKI-resistant NSCLC remains unclear. MicroRNAs (miRNAs) negatively regulate gene expression and their dysregulation has been implicated in tumorigenesis. To understand the role of microRNAs in TKI-resistant NSCLC, we examined TK receptor-mediated microRNA changes. Here we report that miR-30b/c and miR-221/222, modulated by both EGF and MET receptors, and miR-103, -203, controlled only by MET, play important roles in gefitinib-induced apoptosis and epithelial-mesenchymal transition (EMT) of NSCLC cells, in vitro and in vivo, by inhibiting the expression of Bim, APAF-1, PKC-ε and SRC genes. The finding suggests that modulation of specific microRNAs may provide a therapeutic approach for future treatment of NSCLC

Pharmacological Potential of Cilostazol for Alzheimer’s Disease

Alzheimer’s disease (AD), a slow progressive form of dementia, is clinically characterized by cognitive dysfunction and memory impairment and neuropathologically characterized by the accumulation of extracellular plaques containing amyloid β-protein (Aβ) and neurofibrillary tangles containing tau in the brain, with neuronal degeneration and high level of oxidative stress. The current treatments for AD, e.g., acetylcholinesterase inhibitors (AChEIs), have efficacies limited to symptom improvement. Although there are various approaches to the disease modifying therapies of AD, none of them can be used alone for actual treatment, and combination therapy may be needed for amelioration of the progression. There are reports that cilostazol (CSZ) suppressed cognitive decline progression in patients with mild cognitive impairment or stable AD receiving AChEIs. Previously, we showed that CSZ suppressed Aβ-induced neurotoxicity in SH-SY5Y cells via coincident inhibition of oxidative stress, as demonstrated by reduced activity of nicotinamide adenine dinucleotide phosphate oxidase, accumulation of reactive oxygen species, and signaling of mitogen-activated protein kinase. CSZ also rescued cognitive impairment and promoted soluble Aβ clearance in a mouse model of cerebral amyloid angiopathy. Mature Aβ fibrils have long been considered the primary neurodegenerative factors in AD; however, recent evidence indicates soluble oligomers to initiate the neuronal and synaptic dysfunction related to AD and other protein-misfolding diseases. Further underscoring the potential of CSZ for AD treatment, we recently described the inhibitory effects of CSZ on Aβ oligomerization and aggregation in vitro. In this review, we discuss the possibility of CSZ as a potential disease-modifying therapy for the prevention or delay of AD

Long-stay Tourism : Elderly Japanese Tourists in the Cameron Highlands, Malaysia

Articledepartmental bulletin pape

Anti-Aggregation Effects of Phenolic Compounds on α-Synuclein

The aggregation and deposition of α-synuclein (αS) are major pathologic features of Parkinson\u27s disease, dementia with Lewy bodies, and other α-synucleinopathies. The propagation of αS pathology in the brain plays a key role in the onset and progression of clinical phenotypes. Thus, there is increasing interest in developing strategies that attenuate αS aggregation and propagation. Based on cumulative evidence that αS oligomers are neurotoxic and critical species in the pathogenesis of α-synucleinopathies, we and other groups reported that phenolic compounds inhibit αS aggregation including oligomerization, thereby ameliorating αS oligomer-induced cellular and synaptic toxicities. Heterogeneity in gut microbiota may influence the efficacy of dietary polyphenol metabolism. Our recent studies on the brain-penetrating polyphenolic acids 3-hydroxybenzoic acid (3-HBA), 3,4-dihydroxybenzoic acid (3,4-diHBA), and 3-hydroxyphenylacetic acid (3-HPPA), which are derived from gut microbiota-based metabolism of dietary polyphenols, demonstrated an in vitro ability to inhibit αS oligomerization and mediate aggregated αS-induced neurotoxicity. Additionally, 3-HPPA, 3,4-diHBA, 3-HBA, and 4-hydroxybenzoic acid significantly attenuated intracellular αS seeding aggregation in a cell-based system. This review focuses on recent research developments regarding neuroprotective properties, especially anti-αS aggregation effects, of phenolic compounds and their metabolites by the gut microbiome, including our findings in the pathogenesis of α-synucleinopathies

Long-Stay Tourism and International Retirement Migration : Japanese Retirees in Malaysia

departmental bulletin pape