Holliday junction resolvase in Schizosaccharomyces pombe has identical endonuclease activity to the CCE1 homologue YDC2

A novel Holliday junction resolving activity has been identified in fractionated cell extracts of the fission yeast Schizosaccharomyces pombe . The enzyme catalyses endonucleolytic cleavage of Holliday junction-containing chi DNA and synthetic four-way DNA junctions. The activity cuts with high specificity a synthetic four-way junction containing a 12 bp core of homologous sequences but has no activity on another four-way junction (with a fixed crossover point), a three-way junction, linear duplex DNA or duplex DNA containing six mismatched nucleotides in the centre. The major cleavage sites map as single nicks in the vicinity of the crossover point, 3' of a thymidine residue. These data indicate that the activity has a strong DNA structure selectivity as well as a limited sequence preference; features similar to the Holliday junction resolving enzymes RuvC of Escherichia coli and the mitochondrial CCE1 (cruciform-cuttingenzyme 1) of Saccharomyces cerevisiae. A putative homologue of CCE1 in S.pombe (YDC2_SCHPO) has been identified through a search of the sequence database. The open reading frame of this gene has been cloned and the encoded protein, YDC2, expressed in E.coli . The purified recombinant YDC2 exhibits Holliday junction resolvase activity and is, therefore, a functional S.pombe homologue of CCE1. The resolvase YDC2 shows the same substrate specificity and produces identical cleavage sites as the activity obtained from S. pombe cells. Both YDC2 and the cellular activity cleave Holliday junctions in both orientations to give nicks that can be ligated in vitro. The partially purified Holliday junction resolving enzyme in fission yeast is biochemically indistinguishable from recombinant YDC2 and appears to be the same protein

Why do we observe a weak force? The hierarchy problem in the multiverse

Unless the scale of electroweak symmetry breaking is stabilized dynamically, most of the universes in a multiverse theory will lack an observable weak nuclear interaction. Such "weakless universes" could support intelligent life based on organic chemistry, as long as other parameters are properly adjusted. By taking into account the seemingly-unrelated flavor dynamics that address the hierarchy of quark masses and mixings, we show that such weakless (but hospitable) universes can be far more common than universes like ours. The gauge hierarchy problem therefore calls for a dynamical (rather than anthropic) solution.Comment: 5 pages, 1 figure. v2: Minor modifications, reference added. v3: Minor clarifications; matches published versio

Financing Ecological Farming in Africa : A Guide For International Donors

This report provides a resource to the donor community to facilitate the provision of support to ecological farming across Africa. Donor is defined broadly including: governments providing bilateral overseas development assistance, multilateral financial institutions, philanthropies, and international (UN) development organisations.It focuses on four primary channels as effective conduits for scaling up investment into ecological farming: academic and public research and training institutions; communityseed banks and exchange networks; public procurement schemes and producer organisations and cooperatives. It analysed eleven ecological farming initiatives from around the world involving support from donor organisations

Implications of the CDF t \bar t Forward-Backward Asymmetry for Hard Top Physics

The CDF collaboration has recently reported a large deviation from the standard model of the t \bar t forward-backward asymmetry in the high invariant mass region. We interpret this measurement as coming from new physics at a heavy scale Lambda, and perform a model-independent analysis up to O(1/Lambda^4). A simple formalism to test and constrain models of new physics is provided. We find that a large asymmetry cannot be accommodated by heavy new physics that does not interfere with the standard model. We show that a smoking gun test for the heavy new physics hypothesis is a significant deviation from the standard model prediction for the t \bar t differential cross section at large invariant mass. At M_{t\bar t}>1 TeV the cross section is predicted to be at least twice that of the SM at the Tevatron, and for M_{t\bar t}>1.5 TeV at least three times larger than the SM at the LHC.Comment: 12 pages, 3 figures. v2: Minor corrections in the text, references added. v3: Extended discussion on QCD NLO corrections, added short discussion on LHC dijet constraints; matches published versio

Type 1 diabetes

Type 1 diabetes is a chronic autoimmune disease characterised by insulin deficiency and resultant hyperglycaemia. Knowledge of type 1 diabetes has rapidly increased over the past 25 years, resulting in a broad understanding about many aspects of the disease, including its genetics, epidemiology, immune and β-cell phenotypes, and disease burden. Interventions to preserve β cells have been tested, and several methods to improve clinical disease management have been assessed. However, wide gaps still exist in our understanding of type 1 diabetes and our ability to standardise clinical care and decrease disease-associated complications and burden. This Seminar gives an overview of the current understanding of the disease and potential future directions for research and care

Atmospheric Abundances, Trends and Emissions of CFC-216ba, CFC-216ca and HCFC-225ca

The first observations of the feedstocks, CFC-216ba (1,2-dichlorohexafluoropropane) and CFC-216ca (1,3-dichlorohexafluoropropane), as well as the CFC substitute HCFC-225ca (3,3-dichloro-1,1,1,2,2-pentafluoropropane), are reported in air samples collected between 1978 and 2012 at Cape Grim, Tasmania. Present day (2012) mixing ratios are 37.8 ± 0.08 ppq (parts per quadrillion; 1015) and 20.2 ± 0.3 ppq for CFC-216ba and CFC-216ca, respectively. The abundance of CFC-216ba has been approximately constant for the past 20 years, whilst that of CFC-216ca is increasing, at a current rate of 0.2 ppq/year. Upper tropospheric air samples collected in 2013 suggest a further continuation of this trend. Inferred annual emissions peaked 421 at 0.18 Gg/year (CFC-216ba) and 0.05 Gg/year (CFC-216ca) in the mid-1980s and then decreased sharply as expected from the Montreal Protocol phase-out schedule for CFCs. The atmospheric trend of CFC-216ca and CFC-216ba translates into continuing emissions of around 0.01 Gg/year in 2011, indicating that significant banks still exist or that they are still being used. HCFC-225ca was not detected in air samples collected before 1992. The highest mixing ratio of 52 ± 1 ppq was observed in 2001. Increasing annual emissions were found in the 1990s (i.e., when HCFC-225ca was being introduced as a replacement for CFCs). Emissions peaked around 1999 at about 1.51 Gg/year. In accordance with the Montreal Protocol, restrictions on HCFC consumption and the short lifetime of HCFC-225ca, mixing ratios declined after 2001 to 23.3 ± 0.7 ppq by 2012

Lessons from Recent Measurements of D-\bar D Mixing

An impressive progress in measurements of the D-\bar D mixing parameters has been made in recent years. We explore the implications of these measurements to models of new physics, especially in view of recent upper bounds on the amount of CP violation. We update the constraints on non-renormalizable four-quark operators. We show that the experiments are close to probing minimally flavor violating models with large tan beta. The data challenge models with a scale of order TeV where the flavor violation in the down sector is suppressed by alignment and, in particular, certain classes of supersymmetric models and of warped extra dimension models.Comment: 20 pages, 1 figure. Added references, minor corrections and clarifications. Matches published versio

The spatial variations of lightning during small Florida thunderstorms

Networks of field mills (FM's) and lightning direction finders (LDF's) were used to locate lightning over the NASA KSC on three storm days. Over 90 percent of all cloud-to-ground (CG) flashes that were detected by the LDF's in the study area were also detected by the LDF's. About 17 percent of the FM CG events could be fitted to either a monopole or a dipole charge model. These projected FM charge locations are compared to LDF locations, i.e., the ground strike points. It was found that 95 percent of the LDF points are within 12 km of the FM charge, 75 percent are within 8 km, and 50 percent are within 4 km. For a storm on 22 Jul. 1988, there was a systematic 5.6 km shift between the FM charge centers and the LDF strike points that might have been caused by the meteorological structure of the storm