Metataxonomic Analysis of the Uterine Microbiota Associated with Low Fertility in Dairy Cows Using Endometrial Tissues Prior to First Artificial Insemination

The deterioration in reproductive performance in association with low fertility leads to significant economic losses on dairy farms. The uterine microbiota has begun to attract attention as a possible cause of unexplained low fertility. We analyzed the uterine microbiota associated with fertility by 16S rRNA gene amplicon sequencing in dairy cows. First, the alpha (Chao1 and Shannon) and beta (unweighted and weighted UniFrac) diversities of 69 cows at four dairy farms that had passed the voluntary waiting period before the first artificial insemination (AI) were analyzed with respect to factors including farm, housing style, feeding management, parity, and AI frequency to conception. Significant differences were observed in the farm, housing style, and feeding management, except parity and AI frequency to conception. The other diversity metrics did not show significant differences in the tested factors. Similar results were obtained for the predicted functional profile. Next, the microbial diversity analysis of 31 cows at a single farm using weighted UniFrac distance matrices revealed a correlation with AI frequency to conception but not with parity. In correlation with AI frequency to conception, the predicted function profile appeared to be slightly modified and a single bacterial taxon, Arcobacter, was detected. The bacterial associations related to fertility were estimated. Considering these, the uterine microbiota in dairy cows can be varied depending on the farm management practices and may become one of the measures for low fertility

Hydrogen sulfide activates TRPA1 and releases 5-HT from epithelioid cells of the chicken thoracic aorta

Epithelioid cells in the chicken thoracic aorta are chemoreceptor cells that release 5-HT in response to hypoxia. It is likely that these cells play a role in chemoreception similar to that of glomus cells in the carotid bodies of mammals. Recently, H2S was reported to be a key mediator of carotid glomus cell responses to hypoxia. The aim of the present study was to reveal the mechanism of action of H2S on 5-HT outflow from chemoreceptor cells in the chicken thoracic aorta. The 5-HT outflow induced by NaHS, an H2S donor, and Na2S3, a polysulfide, was measured by using a HPLC equipped with an electrochemical detector. NaHS (0.3-3 mM) caused a concentration-dependent increase in 5-HT outflow, which was significantly inhibited by the removal of extracellular Ca2+. outflow induced by NaHS (0.3 mM) was also significantly inhibited by voltage-dependent L- and N-type Ca2+ channel blockers and a selective TRPA1 channel blocker. Cinnamaldehyde, a TRPA1 agonist, mimicked the secretory response to H2S. 5-HT outflow induced by Na2S3 (10 M) was also inhibited by the TRPA1 channel blocker. Furthermore, the expression of TRPA1 was localized to 5-HT-containing chemoreceptor cells in the aortic wall. These findings suggest that the activation of TRPA1 and voltage-dependent Ca2+ channels is involved in H2S-evoked 5-HT release from chemoreceptor cells in the chicken aorta. (C) 2016 Elsevier Inc. All rights reserved

Molecular Pathology of Murine Ureteritis Causing Obstructive Uropathy with Hydronephrosis

Primary causes of urinary tract obstruction that induces urine retention and results in hydronephrosis include uroliths, inflammation, and tumors. In this study, we analyzed the molecular pathology of ureteritis causing hydronephrosis in laboratory rodents

Spontaneous Regression of Hepatocellular Carcinoma with Portal Vein Tumor Thrombus

An 83-year-old man underwent transcatheter arterial chemoembolization (TACE) for a 20-mm hepatocellular carcinoma (HCC) in Couinaud’s segment 4. Computed tomography (CT) 4 months after TACE showed tumor thrombus in the portal vein in addition to diffuse metastases and arterioportal shunts in the left lobe. Although we performed the best supportive care, the tumor thrombus in the portal vein and tumors in the left lobe had completely disappeared on CT 16 months after the TACE. Rapidly grown portal vein tumor thrombus and arterioportal shunt might be the causes of spontaneous regression of HCC, probably associated with tumor hypoxia

Decrease in an inwardly rectifying potassium conductance in mouse mammary secretory cells after forced weaning

application/pdfMammary glands are physiologically active in female mammals only during nursing. Immediately after weaning, most lactation-related genes are downregulated and milk production ceases. In our previous study, we have detected an inwardly rectifying potassium channel (Kir) 2.1-like current in mammary secretory (MS) cells freshly isolated from lactating mice. This current is highly sensitive to external Ba2+ . The potassium permeability of the Kir channels may contribute to the secretion and/or preservation of ions in milk. We hypothesized that the functions of the Kir channels in MS cells are regulated after weaning. To test this hypothesis, we examined the effect of forced weaning on the Ba2+ -sensitive Kir current and Kir2.1 expression in the mouse mammary glands. Twenty-four hours after weaning, the lumina of mammary acini were histologically enlarged by milk accumulation. The whole-cell patch-clamp analyses showed that the Ba2+ -sensitive Kir current in the post-weaning MS cells was smaller than in the lactating MS cells. The inward conductances of the current in the lactating and post-weaning cells were 4.25 ± 0.77 and 0.93 ± 0.34 nS, respectively. Furthermore, real-time PCR and Western blot analyses showed that Kir2.1 mRNA and protein expression decreased in the post-weaning mammary gland (mRNA, 90% reduction; protein, 47% reduction). Moreover, the local milk accumulation caused by teat sealing decreased Kir conductance in MS cells (2.74 ± 0.45 and 0.36 ± 0.27 nS for control and sealed mammary glands, respectively). This was concomitant with the reduction in the Kir2.1 mRNA expression. Our results suggest that milk stasis after weaning immediately decreases the Kir conductance in MS cells. This decrease in the Kir conductance may be partly caused by the reduction in the Kir2.1 mRNA and protein expression. These alterations during the post-weaning period may be involved in the cessation of ion secretion and/or preservation in the milk.journal articl

Quantitative and Qualitative Urinary Cellular Patterns Correlate with Progression of Murine Glomerulonephritis

The kidney is a nonregenerative organ composed of numerous functional nephrons and collecting ducts (CDs). Glomerular and tubulointerstitial damages decrease the number of functional nephrons and cause anatomical and physiological alterations resulting in renal dysfunction. It has recently been reported that nephron constituent cells are dropped into the urine in several pathological conditions associated with renal functional deterioration. We investigated the quantitative and qualitative urinary cellular patterns in a murine glomerulonephritis model and elucidated the correlation between cellular patterns and renal pathology

LEAP-2017 Simulation Exercise: Calibration of Constitutive Models and Simulation of the Element Tests

This paper presents a summary of the element test simulations (calibration simulations) submitted by 11 numerical simulation (prediction) teams that participated in the LEAP-2017 prediction exercise. A significant number of monotonic and cyclic triaxial (Vasko, An investigation into the behavior of Ottawa sand through monotonic and cyclic shear tests. Masters Thesis, The George Washington University, 2015; Vasko et al., LEAP-GWU-2015 Laboratory Tests. DesignSafe-CI, Dataset, 2018; El Ghoraiby et al., LEAP 2017: Soil characterization and element tests for Ottawa F65 sand. The George Washington University, Washington, DC, 2017; El Ghoraiby et al., LEAP-2017 GWU Laboratory Tests. DesignSafe-CI, Dataset, 2018; El Ghoraiby et al., Physical and mechanical properties of Ottawa F65 Sand. In B. Kutter et al. (Eds.), Model tests and numerical simulations of liquefaction and lateral spreading: LEAP-UCD-2017. New York: Springer, 2019) and direct simple shear tests (Bastidas, Ottawa F-65 Sand Characterization. PhD Dissertation, University of California, Davis, 2016) are available for Ottawa F-65 sand. The focus of this element test simulation exercise is to assess the performance of the constitutive models used by participating team in simulating the results of undrained stress-controlled cyclic triaxial tests on Ottawa F-65 sand for three different void ratios (El Ghoraiby et al., LEAP 2017: Soil characterization and element tests for Ottawa F65 sand. The George Washington University, Washington, DC, 2017; El Ghoraiby et al., LEAP-2017 GWU Laboratory Tests. DesignSafe-CI, Dataset, 2018; El Ghoraiby et al., Physical and mechanical properties of Ottawa F65 Sand. In B. Kutter et al. (Eds.), Model tests and numerical simulations of liquefaction and lateral spreading: LEAP-UCD-2017. New York: Springer, 2019). The simulated stress paths, stress strain responses, and liquefaction strength curves show that majority of the models used in this exercise are able to provide a reasonably good match to liquefaction strength curves for the highest void ratio (0.585) but the differences between the simulations and experiments become larger for the lower void ratios (0.542 and 0.515)

LEAP-2017: Comparison of the Type-B Numerical Simulations with Centrifuge Test Results

This paper presents comparisons of 11 sets of Type-B numerical simulations with the results of a selected set of centrifuge tests conducted in the LEAP-2017 project. Time histories of accelerations, excess pore water pressures, and lateral displacement of the ground surface are compared to the results of nine centrifuge tests. A number of numerical simulations showed trends similar to those observed in the experiments. While achieving a close match to all measured responses (accelerations, pore pressures, and displacements) is quite challenging, the numerical simulations show promising capabilities that can be further improved with the availability of additional high-quality experimental results