Tratamento de imagens landsat para identificação de açaizal em floresta de várzea.

O estuário amazônico é formado por um emaranhado de ilhas, onde existem as florestas de várzeas, áreas de especial importância devido aos elevados valores de produtividade e fertilidade dos solos. Entre os produtos não madeireiros mais importantes dessas áreas destaca-se o açaizeiro (Euterpe oleracea). Nos últimos anos verifica-se uma grande expansão dos açaizais como resultado do crescimento do mercado do açaí no estado do Amapá. Tal expansão pode ser explicada tanto pelo plantio de novas áreas como pelo manejo de açaizais nativos, resultando no aumento da densidade de indivíduos de açaí e redução dos indivíduos das espécies florestais. O mapeamento realizado por meio de imagens de sensoriamento remoto, ainda que com limitações, pode facilitar a avaliação mais aprofundada das áreas de várzeas, tendo em conta o potencial produtivo da mesma. O presente estudo tem como foco os açaizais, nativos e manejados, concentrados na foz do rio Mazagão Velho, uma área de várzea localizada no sudeste do município de Mazagão, estado do Amapá, no estuário do rio Amazonas, onde estão estabelecidos transectos para estudo de manejo florestal e ecologia em áreas de várzea por meio do projeto Florestam executado pela EMBRAPA-Amapá. Para o estudo utilizou-se imagens Landsat 5/TM, do ano 2004 e 2008, selecionadas considerando a menor cobertura de nuvens possível, e imagem Landsat 8/OLI para o ano 2013. O processamento digital das imagens incluiu correção geométrica, fusão entre imagens de 30 e 15m, transformação para imagens-fração de vegetação, solo e agua pura, seguido da aplicação de técnicas de segmentação e classificação por região, com a utilização dos softwares SPRING e ENVI. O mapa resultante da classificação foi organizado em classes de cobertura de solo: floresta de várzea, açaizal em várzea, solo exposto e água. A validação do mapa foi estimada a partir de informações coletadas em campo: pontos de descrição da classe de cobertura de açaizal em várzea. Esses pontos permitiram a geração da matriz de confusão para avaliar a qualidade da classificação. O trabalho apresenta o mapa resultante do processamento das imagens e os resultados mostram que a cobertura de açaizal em várzea sofreu aumento significativo nos últimos anos.GC102

Correspondenceless 3D-2D registration based on expectation conditional maximization

Cum Laude Poster AwardConference Theme: Visualization, Image-Guided Procedures, and Modeling3D-2D registration is a fundamental task in image guided interventions. Due to the physics of the X-ray imaging, however, traditional point based methods meet new challenges, where the local point features are indistinguishable, creating difficulties in establishing correspondence between 2D image feature points and 3D model points. In this paper, we propose a novel method to accomplish 3D-2D registration without known correspondences. Given a set of 3D and 2D unmatched points, this is achieved by introducing correspondence probabilities that we model as a mixture model. By casting it into the expectation conditional maximization framework, without establishing one-to-one point correspondences, we can iteratively refine the registration parameters. The method has been tested on 100 real X-ray images. The experiments showed that the proposed method accurately estimated the rotations (< 1°) and in-plane (X-Y plane) translations (< 1 mm). © 2011 SPIE.published_or_final_versionThe SPIE Medical Imaging 2011, Lake Buena Vista, FL., 12-17 February 2011. In Progress in Biomedical Optics and Imaging, 2011, v. 7964, art. no. 79642

Loss of axonal mitochondria promotes tau-mediated neurodegeneration and Alzheimer\u27s disease-related tau phosphorylation via PAR-1.

Abnormal phosphorylation and toxicity of a microtubule-associated protein tau are involved in the pathogenesis of Alzheimer\u27s disease (AD); however, what pathological conditions trigger tau abnormality in AD is not fully understood. A reduction in the number of mitochondria in the axon has been implicated in AD. In this study, we investigated whether and how loss of axonal mitochondria promotes tau phosphorylation and toxicity in vivo. Using transgenic Drosophila expressing human tau, we found that RNAi-mediated knockdown of milton or Miro, an adaptor protein essential for axonal transport of mitochondria, enhanced human tau-induced neurodegeneration. Tau phosphorylation at an AD-related site Ser262 increased with knockdown of milton or Miro; and partitioning defective-1 (PAR-1), the Drosophila homolog of mammalian microtubule affinity-regulating kinase, mediated this increase of tau phosphorylation. Tau phosphorylation at Ser262 has been reported to promote tau detachment from microtubules, and we found that the levels of microtubule-unbound free tau increased by milton knockdown. Blocking tau phosphorylation at Ser262 site by PAR-1 knockdown or by mutating the Ser262 site to unphosphorylatable alanine suppressed the enhancement of tau-induced neurodegeneration caused by milton knockdown. Furthermore, knockdown of milton or Miro increased the levels of active PAR-1. These results suggest that an increase in tau phosphorylation at Ser262 through PAR-1 contributes to tau-mediated neurodegeneration under a pathological condition in which axonal mitochondria is depleted. Intriguingly, we found that knockdown of milton or Miro alone caused late-onset neurodegeneration in the fly brain, and this neurodegeneration could be suppressed by knockdown of Drosophila tau or PAR-1. Our results suggest that loss of axonal mitochondria may play an important role in tau phosphorylation and toxicity in the pathogenesis of AD

Stabilization of Microtubule-Unbound Tau via Tau Phosphorylation at Ser262/356 by Par-1/MARK Contributes to Augmentation of AD-Related Phosphorylation and Aβ42-Induced Tau Toxicity.

Abnormal accumulation of the microtubule-interacting protein tau is associated with neurodegenerative diseases including Alzheimer\u27s disease (AD). β-amyloid (Aβ) lies upstream of abnormal tau behavior, including detachment from microtubules, phosphorylation at several disease-specific sites, and self-aggregation into toxic tau species in AD brains. To prevent the cascade of events leading to neurodegeneration in AD, it is essential to elucidate the mechanisms underlying the initial events of tau mismetabolism. Currently, however, these mechanisms remain unclear. In this study, using transgenic Drosophila co-expressing human tau and Aβ, we found that tau phosphorylation at AD-related Ser262/356 stabilized microtubule-unbound tau in the early phase of tau mismetabolism, leading to neurodegeneration. Aβ increased the level of tau detached from microtubules, independent of the phosphorylation status at GSK3-targeted SP/TP sites. Such mislocalized tau proteins, especially the less phosphorylated species, were stabilized by phosphorylation at Ser262/356 via PAR-1/MARK. Levels of Ser262 phosphorylation were increased by Aβ42, and blocking this stabilization of tau suppressed Aβ42-mediated augmentation of tau toxicity and an increase in the levels of tau phosphorylation at the SP/TP site Thr231, suggesting that this process may be involved in AD pathogenesis. In contrast to PAR-1/MARK, blocking tau phosphorylation at SP/TP sites by knockdown of Sgg/GSK3 did not reduce tau levels, suppress tau mislocalization to the cytosol, or diminish Aβ-mediated augmentation of tau toxicity. These results suggest that stabilization of microtubule-unbound tau by phosphorylation at Ser262/356 via the PAR-1/MARK may act in the initial steps of tau mismetabolism in AD pathogenesis, and that such tau species may represent a potential therapeutic target for AD

Multilayer gas cells for sub-Doppler spectroscopy

We have carried out theoretical research on ultra-high resolution spectroscopy of atoms (or molecules) in the suggested cell with a series of plane-parallel thin gas layers between spatially separated gas regions of this cell for optical pumping and probing. It is shown the effective velocity selection of optically pumped atoms because of their specific transit time and collisional relaxation in such a cell, which lead to narrow sub-Doppler resonances in absorption of the probe monochromatic light beam. Resolution of this spectroscopic method is analyzed in cases of stationary and definite nonstationary optical pumping of atoms by the broadband radiation versus geometrical parameters of given cells and pumping intensity. The suggested multilayer gas cell is the compact analog of many parallel atomic (molecular) beams and may be used also as the basis of new compact optical frequency standards of high accuracy.Comment: 12 pages, 4 figure

CANGAROO-III Observation of TeV Gamma Rays from the vicinity of PSR B1 706-44

Observation by the CANGAROO-III stereoscopic system of the Imaging Cherenkov Telescope has detected extended emission of TeV gamma rays in the vicinity of the pulsar PSR B1706$-$44. The strength of the signal observed as gamma-ray-like events varies when we apply different ways of emulating background events. The reason for such uncertainties is argued in relevance to gamma-rays embedded in the "off-source data", that is, unknown sources and diffuse emission in the Galactic plane, namely, the existence of a complex structure of TeV gamma-ray emission around PSR B1706$-$44.Comment: 10 pages, 13 figures, to be published in Ap