Scheepswrak aan het Wrakkenpad.:Waardestellend onderzoek van scheepswrak B 36, gemeente Noordoostpolder

Standaardrapport van waardestellend onderzoek aan scheepswrak B 36, gemeente Noordoostpolde

Three DNA polymerases, recruited by different mechanisms, carry out NER repair synthesis in human cells

Nucleotide excision repair (NER) is the most versatile DNA repair system that deals with the major UV photoproducts in DNA, as well as many other DNA adducts. The early steps of NER are well understood, whereas the later steps of repair synthesis and ligation are not. In particular, which polymerases are definitely involved in repair synthesis and how they are recruited to the damaged sites has not yet been established. We report that, in human fibroblasts, approximately half of the repair synthesis requires both polκ and polδ, and both polymerases can be recovered in the same repair complexes. Polκ is recruited to repair sites by ubiquitinated PCNA and XRCC1 and polδ by the classical replication factor complex RFC1-RFC, together with a polymerase accessory factor, p66, and unmodified PCNA. The remaining repair synthesis is dependent on polɛ, recruitment of which is dependent on the alternative clamp loader CTF18-RFC

Factors associated with work ability in patients with chronic whiplash-associated disorder grade II-III: a cross-sectional analysis

Objective: To investigate the factors related to self-perceived work ability in patients with chronic whiplash-associated disorder grades II-III. Design: Cross-sectional analysis. Patients: A total of 166 working age patients with chronic whiplash-associated disorder. Methods: A comprehensive survey collected data on work ability (using the Work Ability Index); demographic, psychosocial, personal, work- and condition-related factors. Forward, stepwise regression modelling was used to assess the factors related to work ability. Results: The proportion of patients in each work ability category were as follows: poor (12.7%); moderate (39.8%); good (38.5%); excellent (9%). Seven factors explained 65% (adjusted R2= 0.65, p < 0.01) of the variance in work ability. In descending order of strength of association, these factors are: greater neck disability due to pain; reduced self-rated health status and health-related quality of life; increased frequency of concentration problems; poor workplace satisfaction; lower self-efficacy for performing daily tasks; and greater work-related stress. Conclusion: Condition-specific and psychosocial factors are associated with self-perceived work ability of individuals with chronic whiplash-associated disorder

Aanloop Molengat – Maritime archaeology and intermediate trade during the Thirty Years’ War

In 1635 or shortly thereafter, a Dutch ship was laden with all sorts of materials and products, mostly metals, but also textiles from the booming wool industries in both Flanders and Holland, a shipment of leather and exotic ivory. It was a ship of considerable size (at least 300 last) and departed from the Dutch Republic at a time of profound troubles. The Eighty Years’ War between the Republic and Spain was far from settled. War at sea was unremitting and intensifying, with Dunkirk privateers an unruly menace to Dutch shipping. Spanish rule in the southern Low Countries was highly militarised, and constant campaigns were waged against it from the North. Central Europe was devastated by the Thirty Years’ War, which had entered a new phase through new alliances. The heavy and strategically valuable cargo of the Dutch ship was assembled from North and South, as well as from a range of places in central Europe. The ship departed for a destination that it never reached. It sank off the coast of Texel, where it was discovered 350 years later. From 1985 to 1999 the wreck site and finds were subject to archaeological research, producing information on the ship, its setting and historical context as well as on the production and distribution of the individual shipments in the cargo, and informing us about the structure of early modern industry and trade, operating despite and because of the war. The present study, initiated by Wilma Gijsbers in 2010 and supported by the Cultural Heritage Agency of the Netherlands (RCE), the Maritime Archaeology Programme at the University of Southern Denmark (MAP-SDU) and the Netherlands Organisation for Scientific Research (NWO; a one-year Odyssee grant), is the first to bring together all this evidence and evaluate it as a whole. Central to the study is the analysis of the ship and cargo assemblage as excavated, which is presented in Part 2 and 3 of this article. This is combined with an analysis of the discovery, its impact and the efficiency of fieldwork methodology in Part 1, and with reflections on the contribution the project makes to our understanding of production, trade and international relations in the specific historical context in Part 4

Aanloop Molengat – Maritime archaeology and intermediate trade during the Thirty Years’ War

In 1635 or shortly thereafter, a Dutch ship was laden with all sorts of materials and products, mostly metals, but also textiles from the booming wool industries in both Flanders and Holland, a shipment of leather and exotic ivory. It was a ship of considerable size (at least 300 last) and departed from the Dutch Republic at a time of profound troubles. The Eighty Years’ War between the Republic and Spain was far from settled. War at sea was unremitting and intensifying, with Dunkirk privateers an unruly menace to Dutch shipping. Spanish rule in the southern Low Countries was highly militarised, and constant campaigns were waged against it from the North. Central Europe was devastated by the Thirty Years’ War, which had entered a new phase through new alliances. The heavy and strategically valuable cargo of the Dutch ship was assembled from North and South, as well as from a range of places in central Europe. The ship departed for a destination that it never reached. It sank off the coast of Texel, where it was discovered 350 years later. From 1985 to 1999 the wreck site and finds were subject to archaeological research, producing information on the ship, its setting and historical context as well as on the production and distribution of the individual shipments in the cargo, and informing us about the structure of early modern industry and trade, operating despite and because of the war. The present study, initiated by Wilma Gijsbers in 2010 and supported by the Cultural Heritage Agency of the Netherlands (RCE), the Maritime Archaeology Programme at the University of Southern Denmark (MAP-SDU) and the Netherlands Organisation for Scientific Research (NWO; a one-year Odyssee grant), is the first to bring together all this evidence and evaluate it as a whole. Central to the study is the analysis of the ship and cargo assemblage as excavated, which is presented in Part 2 and 3 of this article. This is combined with an analysis of the discovery, its impact and the efficiency of fieldwork methodology in Part 1, and with reflections on the contribution the project makes to our understanding of production, trade and international relations in the specific historical context in Part 4

Aanloop Molengat – Maritime archaeology and intermediate trade during the Thirty Years’ War

In 1635 or shortly thereafter, a Dutch ship was laden with all sorts of materials and products, mostly metals, but also textiles from the booming wool industries in both Flanders and Holland, a shipment of leather and exotic ivory. It was a ship of considerable size (at least 300 last) and departed from the Dutch Republic at a time of profound troubles. The Eighty Years’ War between the Republic and Spain was far from settled. War at sea was unremitting and intensifying, with Dunkirk privateers an unruly menace to Dutch shipping. Spanish rule in the southern Low Countries was highly militarised, and constant campaigns were waged against it from the North. Central Europe was devastated by the Thirty Years’ War, which had entered a new phase through new alliances. The heavy and strategically valuable cargo of the Dutch ship was assembled from North and South, as well as from a range of places in central Europe. The ship departed for a destination that it never reached. It sank off the coast of Texel, where it was discovered 350 years later. From 1985 to 1999 the wreck site and finds were subject to archaeological research, producing information on the ship, its setting and historical context as well as on the production and distribution of the individual shipments in the cargo, and informing us about the structure of early modern industry and trade, operating despite and because of the war. The present study, initiated by Wilma Gijsbers in 2010 and supported by the Cultural Heritage Agency of the Netherlands (RCE), the Maritime Archaeology Programme at the University of Southern Denmark (MAP-SDU) and the Netherlands Organisation for Scientific Research (NWO; a one-year Odyssee grant), is the first to bring together all this evidence and evaluate it as a whole. Central to the study is the analysis of the ship and cargo assemblage as excavated, which is presented in Part 2 and 3 of this article. This is combined with an analysis of the discovery, its impact and the efficiency of fieldwork methodology in Part 1, and with reflections on the contribution the project makes to our understanding of production, trade and international relations in the specific historical context in Part 4