PCR-based sex determination of cetaceans and dugong from the Indian seas

A sex-determination technique based on PCR amplifi- cation of genomic DNA extracted from the skin tissue has been standardized in cetaceans and dugong sam-pled from the Indian seas. A Y-chromosome-specific region (SRY or Sex-determining Y-chromosome gene) of 210–224 bp size in the genome has been amplified (only in males) using specific PCR primers. A fragment of the ZFX/ZFY (zinc finger protein genes located both on the X and Y chromosomes respectively) re-gion in the size range 442–445 bp is also amplified (in both sexes) using another pair of primers simultaneously as positive controls for confirmation of sex. Molecular sexing was standardized in spinner dolphin (Stenella longirostris), bridled dolphin (Stenella attenuata), bottlenose dolphin (Tursiops aduncus), Indo-Pacific humpbacked dolphin (Sousa chinensis), Risso’s dolphin (Grampus griseus), finless porpoise (Neopho-caena phocaenoides), sperm whale (Physeter macro-cephalus), blue whale (Balaenoptera musculus), Bryde’s whale (Balaenoptera edeni) and dugong (Dugong du-gon), which are all vulnerable/endangered species pro- tected under the Indian Wildlife Act

EVALUATING THE CLINICAL EFFICACY OF A POLYHERBAL FORMULATION AROGH PLUS ON STRESS – A RANDOMISED CLINICAL STUDY

Background : Stress is a common entity, widely spoken about among the working population, for which a safe and effective remedy is needed.Purpose of the study : To evaluate the clinical effectiveness of Arogh plus a polyherbal formulation, towards stress relieving properties in a randomized clinical trial in volunteers.Results : Ten volunteers from Marketing field, evaluated to be under stress, completed the full course. All were clinically evaluated based upon symptoms, anthropometric evaluation, hematological, diabetic and serum cortisol and urine profile. All the parameters were evaluated during 0 day, 15th day, 30th day and on 45th day after stopping the drug internally. Three grams of Arogh plus was given twice daily for a period of 30 days was found to decrease symptoms due to stress and the benefits was reinforced by way of significant reduction in serum cortisol with a reduction of 36.99 % within a month.Conclusion: Arogh plus is an effective formulation in relieving stress and improving the quality of life

Molecular Identification of Delphinids and Finless Porpoise (Cetacea) from the Arabian Sea and Bay of Bengal

The exact number of extant delphinid species from seas around India is still debated and the lack of adequate field keys and reliable inventory has resulted in misidentification of several species. As a part of a project to develop a molecular taxonomy of cetaceans from this region, partial sequences of mtDNA cytochrome b were generated from accidentally caught/stranded delphinids and finless porpoise. Species were identified by phylogenetic reconstruction of sample sequences with the reference sequences available in portals GenBank (NCBI) and the web-based program DNA Surveillance. A comparison was made with the homologous sequences of corresponding species from other seas of the world. Our molecular investigations allowed us to identify five species of cetaceans from Indian coasts, including Delphinus capensis, previously reported as D. delphis. We detected unique haplotypes in Indo pacific humpbacked dolphin (Sousa chinensis; n = 2) and finless porpoise (Neophocaena phocaenoides; n = 12) from Indian coast. On the other hand, some haplotypes were shared with other regional populations in spinner dolphin (Stenella longirostris; n = 16) and bottlenose dolphin (Tursiops aduncus; n = 3). Common dolphins (Delphinus capensis; n = 2) had both unique and shared haplotypes including one highly divergent sequence

A note on observations on cetaceans in the western Indian sector of the Southern Ocean (20-56°S and 45-57°30’E), January to March 2004

A multi-disciplinary and multi-institutional pilot expedition was organised by the National Centre for Antarctic and Ocean Research (NCAOR) to the ice-free areas of the Western Indian Ocean sector of the Southern Ocean onboard ORV Sagar Kanya during the austral summer of 2004 (January-March). This survey, conducted by the Central Marine Fisheries Research Institute (CMFRI), is the first Indian attempt to survey for cetacean diversity in the Southern Ocean. The ultimate objective is to determine distribution, relative abundance, migration patterns and critical habitat parameters. 68% of a total of 13 sightings (22 individuals) were positively identified and species observed included Antarctic minke whales (Balaenoptera bonaerensis), fin whales (B. physalus), sei whales (B. borealis) and blue whales (B. musculus). The blue whales were not identified to the sub-species level. The highest concentration of cetaceans was between 35° and 37°S (along 45°E) and between 48° and 53°S (along 45°E). Relatively small numbers of cetaceans were observed during the present cruise, possibly because most of the cetacean sighting effort was made during inclement sea conditions. Results on the sighting characteristics and occurrence patterns of the cetaceans in relation to the region and hydrographical parameters are discussed briefly vis a vis published information from the Southern Ocean

Indian Efforts on the Inventorization of Marine Mammal Species for their Conservation and Management

The present study is the first attempt to use molecular tools for identification of marine mammals in India. The objective was to develop a database of genetic sequences for future marine mammal research in addition to confirming the species identity of cetaceans and dugongs using a molecular approach. Partial sequencing of mitochondrial DNA loci was carried out in accidentally caught/stranded specimens of Spinner dolphin (Stenella longirostris), Pantropical spotted dolphin/bridled dolphin (Stenella attenuata), Bottlenose dolphin (Tursiops aduncus), Long-beaked common dolphin (Delphinus capensis), Indopacific humpbacked dolphin (Sousa chinensis), Risso’s dolphin (Grampus griseus), Finless porpoise (Neophocaena phocaenoides), Sperm whale (Physeter macrocephalus), Blue whale (Balaenoptera musculus), Bryde’s whale (Balaenoptera edeni) and Dugong (Dugong dugon). Molecular identification of species was done by phylogenetic reconstruction of the sequences using portals GenBank and DNA Surveillance. Apart from ratifying their morphological identification, the analysis was able to distinguish specimens that otherwise, could not have been identified using conventional approaches. Phylogenetic analysis of the Sousa-Stenella-Tursiops-Delphinus group indicated more or less robust monophyly for all species in this complex, except Delphinus capensis. A sister-group relationship for Sperm whales and Baleen whales was evident, that would place the former closer to the latter than to any other group of toothed whales

A note on cetacean distribution in the Indian EEZ and contiguous seas during 2003-07

Relatively little is known about the distribution of cetaceans in Indian seas due to lack of systematic surveys. For collecting data on species distribution, 35 opportunistic surveys were conducted onboard FORV Sagar Sampada between October 2003 and February 2007 in the Indian EEZ and contiguous seas. In 5,254 hours of sighting effort, a total of 473 cetacean records were made with 5,865 individuals. The occurrence of 10 species from three cetacean families was confirmed. The Indo-Pacific bottlenose dolphin was the most frequently sighted species, whereas the spinner dolphin was dominant in terms of abundance. Long-beaked common dolphins, Indo-Pacific hump-backed dolphin and sperm whales were also recorded at frequent intervals. Cetaceans were found to have a wide geographical distribution in the Indian EEZ and contiguous seas. High abundance and species richness were recorded in the Southeastern Arabian Sea and southern Sri Lankan waters. From the information collected during the present study, the platform of opportunity has proved to be a useful means for cetacean surve

Stomach contents of cetaceans incidentally caught along Mangalore and Chennai coasts of India

Abstract The stomachs of 32 individuals of seven cetacean species incidentally caught in gill net and purseseine fisheries along Mangalore and Chennai coasts (India) between 2004 and 2006 were examined. The whole stomach (fore-gut, mid-gut and hind-gut) was examined in all cases. Prey remains (666 prey items comprising six species of teleosts, one crustacean and one squid species) were found in the stomachs of eight individuals (the remaining 24 stomachs were found to be empty). All cetaceans were found to feed mostly on teleosts with wide range of trophic levels. Based on an index that included frequency of occurrence, percentage by number and by weight, the oil sardine Sardinella longiceps was the main prey in the sample. Cetaceans appear to favour both pelagic as well as demersal prey, possibly indicating surface and benthic feeding habits