Optimization of capsaicin acylase production from Streptomyces mobaraensis in bench-top fermenter

Capsaicin, the major pungent principle in hot pepper fruit, can be hydrolyzed enzymatically to vanillylamine (a natural precursor of vanillin) using a specific acylase from Streptomyces mobaraensis. Production of this enzyme using strain DSM40847 was studied under batch fermentation conditions in stirred tank (STR) and airlift (AR) bioreactors. The process performance in both fermentation devices was different with respect to biomass, enzyme concentration and specific yield (enzyme activity/biomass content); in particular the specific yield was lower in the AR (5.7 mU/g of biomass) than in the STR (6.25 mU/g of biomass). Experiments carried out in STR bioreactors at controlled (DO = 20% of saturation) and uncontrolled dissolved oxygen concentration, and at constant stirrer speeds (300, 450 and 600 rpm) demonstrated that the DO level has no remarkable effect on the production of the capsaicin-hydrolyzing enzyme, which is mainly produced in a cell-associated

Selection of Co-Substrate and Aeration Conditions for Vanillin Production by Escherichia coli JM109/pBB1

Yeast extract, Luria-Bertani medium and tryptone were tested as co-substrates for vanillin production from ferulic acid by resting cells of Escherichia coli JM109/pBB1. Yeast extract proved to be the best component for sustaining such a bioconversion, which is not self-sustained from the bioenergetic point of view. Tests were also performed under variable aeration conditions by simultaneously varying the ratio of medium to vessel volume and the agitation speed. The results of these tests suggest that, under excess aeration, a non-specific oxidase activity was very likely responsible for the oxidation of a significant portion of vanillin to vanillic acid, thus reducing the vanillin yield

From marine neglected substrata new fungal taxa of potential biotechnological interest: the case of Pelagia noctiluca

Introduction: The marine environment is extremely complex and exerts strong evolutionary pressure often leading to the appearance of microbial strains with new metabolic competencies. Microorganisms in marine ecosystems are still largely unknown and should be explored and conserved for biodiversity preservation, possible ecosystem restoring, and other applications. Biodiversity conservation should become a basic ecological strategy of particular significance in relation to global change. In this context, the present research aimed at exploring the culturable mycobiota associated with the jellyfish Pelagia noctiluca, never studied before. In addition, the isolated strains were tested for potential application (antimicrobial activity and presence of genes related to the production of secondary metabolites). Methods: Five jellyfishes were collected in the coastal area of Giglio Island and processed to isolate epizoic fungi. The strains were identified using a polyphasic approach (morphological, physiological, and molecular) and their salt preference was also investigated. The antifungal and antibacterial activity were tested for each strain with agar plug diffusion test. The presence of some key genes related to the main pathways for the production of secondary metabolites in fungi, polyketide synthases (PKSs), and non-ribosomal peptide synthase (NRPSs), was also assessed. Results: A total of 164 isolates were obtained; after the dereplication, 40 morphotypes, and 23 species were identified. The phylogenetic analyses suggested the presence of new taxa belonging to Pleosporales: two new genera and species, and a new species of Tamaricicola. The detected mycobiota showed a relatively high diversity, if compared to other epizoic fungal communities. All isolated strains were marine fungi as confirmed by their salt preference and marked euryhalinism. The genes related to the two main pathways for the production of secondary metabolites in fungi, PKSs and NRPSs, were identified in four and nine strains, respectively. The antimicrobial activity was revealed in 70% of the strains, including the new taxa. The abundance of bioactive strains may be related to the potential involvement of epizoic fungi in host defense strategies. Moreover, these strains could show a high potential for further biotechnological applications particularly in the case of new taxa. All strains are maintained in culture collections

Long-term heliomagnetic field variation based on cosmogenic 44Ti in meteorites

Reconstructions of the heliospheric magnetic field (HMF) in the past centuries are mainly based on the analysis of sunspot activity, geomagnetic data or on measurement of cosmogenic radioisotopes stored in terrestrial reservoirs (tree rings and ice cores). There are, however, significant discrepancies among the results obtained by various techniques using different proxies of solar magnetic activity. In this work, new results obtained from a unique approach based on the measurement of the cosmogenic 44Ti activity detected in meteorites are presented and compared with the most recent reconstructions of the near-Earth HMF strength. The very low level of 44Ti activity in several meteorites fallen in the last 250 years was determined by using gamma-ray spectrometers (HPGe+NaI) located in the underground laboratory of Monte dei Cappuccini (INAF-OATo) in Torino, Italy. This approach, specifically designed to overcome the main problems affecting other methods, yields a powerful independent tool to reconstruct the long-term evolution of the HMF through the last two and a half centuries

Improvement of the extraction method of faint signals in γ -activity measurements of meteorites

The final publication is available at Springer via https://doi.org/10.1140/epjp/i2017-11556-yAt the underground laboratory of Monte dei Cappuccini (OATo-INAF) in Torino (Italy) we set up selective HPGe-NaI(Tl) spectrometers for measurements of cosmogenic radioisotopes in meteorites in order to study centennial-scale modulation of solar activity. 44 Ti is a suitable proxy for this timescale, but its detection is difficult due to the strong interference by naturally occurring 214 Bi. In order to optimize the extraction of the 44 Ti signal, we have developed software procedures specifically designed to improve selectivity of the Ge-NaI detectors coincidence