HAX-1 overexpression, splicing and cellular localization in tumors

<p>Abstract</p> <p>Background</p> <p>HAX-1 has been described as a protein potentially involved in carcinogenesis and especially metastasis. Its involvement in regulation of apoptosis and cell migration along with some data indicating its overexpression in cancer cell lines and tumors suggests that HAX-1 may play a role in neoplastic transformation. Here we present the first systematic analysis of HAX-1 expression in several solid tumors.</p> <p>Methods</p> <p>Using quantitative RT-PCR, we have determined the mRNA levels of <it>HAX1 </it>splice variant I in several solid tumors. We have also analyzed by semiquantitative and quantitative RT-PCR the expression of five <it>HAX-1 </it>splice variants in breast cancer samples and in normal tissue from the same individuals. Quantitative PCR was also employed to analyze the effect of estrogen on <it>HAX1 </it>expression in breast cancer cell line. Immunohistochemical analysis of HAX-1 was performed on normal and breast cancer samples.</p> <p>Results</p> <p>The results reveal statistically important <it>HAX1 </it>up-regulation in breast cancer, lung cancer and melanoma, along with some minor variations in the splicing pattern. HAX-1 up-regulation in breast cancer samples was confirmed by immunohistochemical analysis, which also revealed an intriguing HAX-1 localization in the nuclei of the tumor cells, associated with strong ER status.</p> <p>Conclusion</p> <p>HAX-1 elevated levels in cancer tissues point to its involvement in neoplastic transformation, especially in breast cancer. The connection between HAX-1 nuclear location and ER status in breast cancer samples remains to be clarified.</p

Cardiopoietic cell therapy for advanced ischemic heart failure: results at 39 weeks of the prospective, randomized, double blind, sham-controlled CHART-1 clinical trial

Cardiopoietic cells, produced through cardiogenic conditioning of patients' mesenchymal stem cells, have shown preliminary efficacy. The Congestive Heart Failure Cardiopoietic Regenerative Therapy (CHART-1) trial aimed to validate cardiopoiesis-based biotherapy in a larger heart failure cohort

Lithium Transference Number Measurements in Anion Trapping Triphenyloborane Composites

Abstract not Available.</jats:p

Discrimination of amino acids by electronic tongue

Obecnie potrzebne są metody szybkiego, dokładnego, a przede wszystkim taniego oznaczania aminokwasów w próbkach biologicznych. Do takich technik należy potencjometryczny elektroniczny język, który pozwala na rozróżnianie próbek ciekłych. W pracy przedstawiono zastosowanie potencjometrycznego elektronicznego języka do klasyfikowania 5 aminokwasów. Sprawdzono możliwość rozpoznania poszczególnych analitów w roztworach o różnych pH, a następnie wykorzystując metodę częściowych najmniejszych kwadratów (PLS) rozróżniono poszczególne aminokwasy.In the last few years medical discoveries showed that presence of amino acids can be associated with many diseases. Usually, these compounds are determined using HPLC method (high performance liquid chromatography), however, due to its noxiousness, new methods are investigated. Application of electronic tongue system can be an alternative method for fast, accurate, affordable determination of amino acids in biological samples. This device is used for analysis and classification of complex liquid samples. Its main advantage is the ability to distinguish between samples containing various components on-line, without prior sample preparation and modification. In this paper there are presented preliminary studies on the recognition of 5 amino acids (glutamic acid, alanine, serine, arginine, aspartic acid) with use of a sensor array composed of ion-selective electrodes of various selectivity. Chemical images of the samples were obtained by measuring the electrode signals in solutions of amino acids for various pH ranging from 2.5 to 11.5. The possibility of identification of individual analytes in solutions of different pH was examined with use of the Partial Least Squares Analysis