Incorporation of cytochrome oxidase into cardiolipin bilayers and induction of nonlamellar phases.

Cytochrome oxidase from beef heart has been lipid-substituted with beef heart cardiolipin. The lipid phase behavior and protein aggregation state of the reconstituted complexes have been studied with 31P NMR, freeze-fracture electron microscopy, and saturation-transfer ESR of the spin-labeled protein. In the absence of salt, the lipid has a lamellar arrangement, and the protein is integrated and uniformly distributed in the membrane vesicles and undergoes rapid rotational diffusion. The presence of the protein stabilizes the cardiolipin lamellar phase against salt-induced transitions to the inverted hexagonal phase. The threshold salt concentration becomes higher and the extent of conversion becomes lower with decreasing lipid:protein ratio. In high salt, lamellar-phase lipid with integrated protein coexists with hexagonal-phase lipid free of protein, and the rotational diffusion of the protein is drastically reduced as a result of the high packing density

Parametric study of transport aircraft systems cost and weight

The results of a NASA study to develop production cost estimating relationships (CERs) and weight estimating relationships (WERs) for commercial and military transport aircraft at the system level are presented. The systems considered correspond to the standard weight groups defined in Military Standard 1374 and are listed. These systems make up a complete aircraft exclusive of engines. The CER for each system (or CERs in several cases) utilize weight as the key parameter. Weights may be determined from detailed weight statements, if available, or by using the WERs developed, which are based on technical and performance characteristics generally available during preliminary design. The CERs that were developed provide a very useful tool for making preliminary estimates of the production cost of an aircraft. Likewise, the WERs provide a very useful tool for making preliminary estimates of the weight of aircraft based on conceptual design information

Underperforming policy networks : the biopesticides network in the United Kingdom

Loosely integrated and incomplete policy networks have been neglected in the literature. They are important to consider in terms of understanding network underperformance. The effective delivery and formulation of policy requires networks that are not incomplete or underperforming. The biopesticides policy network in the United Kingdom is considered and its components identified with an emphasis on the lack of integration of retailers and environmental groups. The nature of the network constrains the actions of its agents and frustrates the achievement of policy goals. A study of this relatively immature policy network also allows for a focus on network formation. The state, via an external central government department, has been a key factor in the development of the network. Therefore, it is important to incorporate such factors more systematically into understandings of network formation. Feedback efforts from policy have increased interactions between productionist actors but the sphere of consumption remains insufficiently articulated

A Radial Velocity Study of CTCV J1300-3052

We present time-resolved spectroscopy of the eclipsing, short period cataclysmic variable CTCV J1300-3052. Using absorption features from the secondary star, we determine the radial velocity semi-amplitude of the secondary star to be K2 = 378 \pm 6 km/s, and its projected rotational velocity to be v sin i = 125 \pm 7 km/s. Using these parameters and Monte Carlo techniques, we obtain masses of M1 = 0.79 \pm 0.05 MSun for the white dwarf primary and M2 = 0.198 \pm 0.029 MSun for the M-type secondary star. These parameters are found to be in excellent agreement with previous mass determinations found via photometric fitting techniques, supporting the accuracy and validity of photometric mass determinations in short period CVs.Comment: Accepted for publication in MNRAS (24th January 2012). 10 pages, 9 figures (black and white

Time-resolved spectroscopy of the pulsating CV GW Lib

We present time-resolved optical spectroscopy of the dwarf nova GW Librae during its rare 2007 April superoutburst and compare these with quiescent epochs. The data provide the first opportunity to track the evolution of the principal spectral features. In the early stages of the outburst, the optically thick disc dominates the optical and the line components show clear orbital radial velocity excursions. In the course of several weeks, optically thin regions become more prominent as strong emission lines replace the broad disc absorption. Post-outburst spectroscopy covering the I band illustrates the advantages of Ca II relative to the commonly used Balmer lines when attempting to constrain binary parameters. Due to the lower ionization energy combined with smaller thermal and shear broadening of these lines, a sharp emission component is seen to be moving in between the accretion disc peaks in the Ca II line. No such component is visible in the Balmer lines. We interpret this as an emission component originating on the hitherto unseen mass donor star. This emission component has a mean velocity of similar to -15 +/- 5 km s(-1) which is associated with the systemic velocity., and a velocity semi-amplitude of K-em = 82.2 +/- 4.9 km s(-1). Doppler tomography reveals an asymmetric accretion disc, with the S-wave mapping to a sharp spot in the tomogram with a velocity consistent to what is obtained with line profile fitting. A centre of symmetry analysis of the disc component suggests a very small value for the WD orbital velocity K-1 as is also inferred from double Gaussian fits to the spectral lines. While our conservative dynamical limits place a hard upper limit on the binary mass ratio of q < 0.23, we favour a significantly lower value near q similar to 0.06. Pulsation modelling suggests a white dwarf mass similar to 1 M-circle dot. This, paired with a low-mass donor, near the empirical sequence of an evolved cataclysmic variable close to the period bounce, appears to be consistent with all the observational constraints to date

KIC 2856960: the impossible triple star

KIC 2856960 is a star in the Kepler field which was observed by Kepler for 4 years. It shows the primary and secondary eclipses of a close binary of 0.258d as well as complex dipping events that last for about 1.5d at a time and recur on a 204d period. The dips are thought to result when the close binary passes across the face of a third star. In this paper we present an attempt to model the dips. Despite the apparent simplicity of the system and strenuous efforts to find a solution, we find that we cannot match the dips with a triple star while satisfying Kepler's laws. The problem is that to match the dips the separation of the close binary has to be larger than possible relative to the outer orbit given the orbital periods. Quadruple star models can get round this problem but require the addition of a so-far undetected intermediate period of order 5 -- 20d that has be a near-perfect integer divisor of the outer 204d period. Although we have no good explanation for KIC 2856960, using the full set of Kepler data we are able to update several of its parameters. We also present a spectrum showing that KIC 2856960 is dominated by light from a K3- or K4-type star.Comment: 11 pages, 13 figures, accepted for publication in MNRAS August 21, 201

Surface tension in bilayer membranes with fixed projected area

We study the elastic response of bilayer membranes with fixed projected area to both stretching and shape deformations. A surface tension is associated to each of these deformations. By using model amphiphilic membranes and computer simulations, we are able to observe both the types of deformation, and thus, both the surface tensions, related to each type of deformation, are measured for the same system. These surface tensions are found to assume different values in the same bilayer membrane: in particular they vanish for different values of the projected area. We introduce a simple theory which relates the two quantities and successfully apply it to the data obtained with computer simulations

Evidence for bimodal orbital separations of white dwarf-red dwarf binary stars

We present the results of a radial velocity survey of 20 white dwarf plus M dwarf binaries selected as a follow up to a \textit{Hubble Space Telescope} study that aimed to spatially resolve suspected binaries. Our candidates are taken from the list of targets that were spatially unresolved with \textit{Hubble}. We have determined the orbital periods for 16 of these compact binary candidates. The period distribution ranges from 0.14 to 9.16\,d and peaks near 0.6\,d. The original sample therefore contains two sets of binaries, wide orbits ($\approx100-1000$\,au) and close orbits ($\lesssim1-10$\,au), with no systems found in the $\approx10-100$\,au range. This observational evidence confirms the bimodal distribution predicted by population models and is also similar to results obtained in previous studies. We find no binary periods in the months to years range, supporting the post common envelope evolution scenario. One of our targets, WD\,1504+546, was discovered to be an eclipsing binary with a period of 0.93\,d

Dissipative Particle Dynamics with energy conservation

Dissipative particle dynamics (DPD) does not conserve energy and this precludes its use in the study of thermal processes in complex fluids. We present here a generalization of DPD that incorporates an internal energy and a temperature variable for each particle. The dissipation induced by the dissipative forces between particles is invested in raising the internal energy of the particles. Thermal conduction occurs by means of (inverse) temperature differences. The model can be viewed as a simplified solver of the fluctuating hydrodynamic equations and opens up the possibility of studying thermal processes in complex fluids with a mesoscopic simulation technique.Comment: 5 page

Dissipative Particle Dynamics with Energy Conservation

The stochastic differential equations for a model of dissipative particle dynamics with both total energy and total momentum conservation in the particle-particle interactions are presented. The corresponding Fokker-Planck equation for the evolution of the probability distribution for the system is deduced together with the corresponding fluctuation-dissipation theorems ensuring that the ab initio chosen equilibrium probability distribution for the relevant variables is a stationary solution. When energy conservation is included, the system can sustain temperature gradients and heat flow can be modeled.Comment: 7 pages, submitted to Europhys. Let