Prothonotary warbler nestling growth and condition inresponse to variation in aquatic and terrestrial preyavailability

Aquatic prey subsidies entering terrestrial habitats are well documented, but little is known about the degree to which these resources provide fitness benefits to riparian consumers. Riparian species take advantage of seasonal pulses of both terrestrial and aquatic prey, although aquatic resources are often over-looked in studies of how diet influences the reproductive ecology of these organisms. Ideally, the timing of resource pulses should occur at the time of highest reproductive demand. This study investigates the availability of aquatic(mayfly) and terrestrial (caterpillar) prey resources as well as the nestling diet of the prothonotary warbler (Protonotaria citrea) at two sites along the lower James River in Virginia during the 2014 breeding season. We found large differences in availability of prey items between the two sites, with one having significantly higher mayfly availability. Nestling diet was generally reflective of prey availability, and nestlings had faster mean growth rates at the site with higher aquatic prey availability. Terrestrial prey were fed more readily at the site with lower aquatic prey availability, and at this site, nestlings fed mayflies had higher mean growth rates than nestlings fed only terrestrial prey. Our results suggest that aquatic subsidies are an important resource for nestling birds and are crucial to understanding the breeding ecology of riparian species

Detection of Pulsed X-ray Emission from PSR B1706-44

We report the first detection of pulsed X-ray emission from the young, energetic radio and Gamma-ray pulsar PSR B1706-44. We find a periodic signal at a frequency of f = 9.7588088 +/- 0.0000026 Hz (at epoch 51585.34104 MJD), consistent with the radio ephemeris, using data obtained with the High Resolution Camera on-board the Chandra X-ray Observatory}. The probability that this detection is a chance occurrence is 3.5E-5 as judged by the Rayleigh test. The folded light curve has a broad, single-peaked profile with a pulsed fraction of 23% +/- 6%. This result is consistent the ROSAT PSPC upper limit of < 18% after allowing for the ability of Chandra to resolve the pulsar from a surrounding synchrotron nebula. We also fitted Chandra spectroscopic data on PSR B1706-44, which require at least two components, e.g., a blackbody of temperature T(infinity) between 1.51E6 K and 1.83E6 K and a power-law of Gamma = 2.0 +/- 0.5. The blackbody radius at the nominal 2.5 kpc distance is only R(infinity) = 3.6 +/- 0.9 km, indicating either a hot region on a cooler surface, or the need for a realistic atmosphere model that would allow a lower temperature and larger area. Because the power-law and blackbody spectra each contribute more than 23% of the observed flux, it is not possible to decide which component is responsible for the modulation in the spectrally unresolved light curve.Comment: 6 pages, 4 figures, Latex, emulateapj. Published version. Includes an updated radio ephemeris and presents the absolute radio/X-ray phase alignmen

Cloning, preparation and preliminary crystallographic studies of penicillin V acylase autoproteolytic processing mutants

The crystallization of three catalytically inactive mutants of penicillin Vacylase (PVA) from Bacillus sphaericus in precursor and processed forms is reported. The mutant proteins crystallize in different primitive monoclinic space groups that are distinct from the crystal forms for the native enzyme. Directed mutants and clone constructs were designed to study the post-translational autoproteolytic processing of PVA. The catalytically inactive mutants will provide threedimensional structures of precursor PVA forms, plus open a route to the study of enzyme-substrate complexes for this industrially important enzyme

Multi-transition study and new detections of class II methanol masers

We have used the ATNF Mopra antenna and the SEST antenna to search in the directions of several class II methanol maser sources for emission from six methanol transitions in the frequency range 85-115 GHz. The transitions were selected from excitation studies as potential maser candidates. Methanol emission at one or more frequencies was detected from five of the maser sources, as well as from Orion KL. Although the lines are weak, we find evidence of maser origin for three new lines in G345.01+1.79, and possibly one new line in G9.62+0.20. The observations, together with published maser observations at other frequencies, are compared with methanol maser modelling for G345.01+1.79 and NGC6334F. We find that the majority of observations in both sources are consistent with a warm dust (175 K) pumping model at hydrogen density ~10^6 cm^-3 and methanol column density ~5 x 10^17 cm^-2. The substantial differences between the maser spectra in the two sources can be attributed to the geometry of the maser region.Comment: 13 pages, 6 figures, Accepted for publication in MNRA

Correlation between X-ray Lightcurve Shape and Radio Arrival Time in the Vela Pulsar

We report the results of simultaneous observations of the Vela pulsar in X-rays and radio from the RXTE satellite and the Mount Pleasant Radio Observatory in Tasmania. We sought correlations between the Vela's X-ray emission and radio arrival times on a pulse by pulse basis. At a confidence level of 99.8% we have found significantly higher flux density in Vela's main X-ray peak during radio pulses that arrived early. This excess flux shifts to the 'trough' following the 2nd X-ray peak during radio pulses that arrive later. Our results suggest that the mechanism producing the radio pulses is intimately connected to the mechanism producing X-rays. Current models using resonant absorption of radio emission in the outer magnetosphere as a cause of the X-ray emission are explored as a possible explanation for the correlation.Comment: 6 pages, 5 figures, accepted by Ap