Extraction and sensitive detection of toxins A and B from the human pathogen Clostridium difficile in 40 seconds using microwave-accelerated metal-enhanced fluorescence.

Clostridium difficile is the primary cause of antibiotic associated diarrhea in humans and is a significant cause of morbidity and mortality. Thus the rapid and accurate identification of this pathogen in clinical samples, such as feces, is a key step in reducing the devastating impact of this disease. The bacterium produces two toxins, A and B, which are thought to be responsible for the majority of the pathology associated with the disease, although the relative contribution of each is currently a subject of debate. For this reason we have developed a rapid detection assay based on microwave-accelerated metal-enhanced fluorescence which is capable of detecting the presence of 10 bacteria in unprocessed human feces within 40 seconds. These promising results suggest that this prototype biosensor has the potential to be developed into a rapid, point of care, real time diagnostic assay for C. difficile

Introducing ‘facilitymetrics’: a first review and analysis of commonly used measures of scientific leadership among synchrotron radiation facilities worldwide

Big Science accelerator complexes are no longer mere tools for nuclear and particle physics, but modern-day experimental resources for a wide range of natural sciences and often named instrumental to scientific and technological development for innovation and economic growth. Facilities compete on a global market to attract the best users and facilitate the best science, and advertise the achievement of their users as markers of quality and productivity. Thus a need has risen for (quantitative) quality assessment of science on the level of facilities. In this article, we examine some quantitative performance measurements frequently used by facilities to display quality: technical reliability, competition for access, and publication records. We report data from the world’s three largest synchrotron radiation facilities from the years 2004–2010, and discuss their meaning and significance by placing them in proper context. While we argue that quality is not possible to completely capture in these quantitative metrics, we acknowledge their apparent importance and, hence, we introduce and propose facilitymetrics as a new feature of the study of modern big science, and as a new empirical focus for scientometrical study, in the hope that future studies can contribute to a deeper, much-needed analysis of the topic

Local Government Revenue Mobilisation in Anglophone Africa

This paper examines opportunities and constraints facing local revenue mobilisation in anglophone Africa, with an emphasis on urban settings. It discusses specific revenue instruments and their effects on economic efficiency, income distribution and accountability. In particular, it addresses political and administrative constraints facing various revenue instruments and factors affecting citizens’ compliance. The analysis is illustrated with examples from across anglophone Africa. A general conclusion emerging from the study is that local revenues mobilised in most local government authorities in Africa are necessary but not sufficient to develop and supply adequate services for the fast-growing population. On this basis, areas for further research on local government revenue mobilisation in Africa are identifiedDfI

Towards Customary Legal Empowerment

Rule of Law and Development: Formation, Implementation and Improvement of Law and Governance in Developing Countrie

Publisher Correction: Stroke genetics informs drug discovery and risk prediction across ancestries (Nature, (2022), 611, 7934, (115-123), 10.1038/s41586-022-05165-3)

In the version of this article initially published, the name of the PRECISE4Q Consortium was misspelled as “PRECISEQ” and has now been amended in the HTML and PDF versions of the article. Further, data in the first column of Supplementary Table 55 were mistakenly shifted and have been corrected in the file accompanying the HTML version of the article

Publisher Correction: Stroke genetics informs drug discovery and risk prediction across ancestries.

In the version of this article initially published, the name of the PRECISE4Q Consortium was misspelled as “PRECISEQ” and has now been amended in the HTML and PDF versions of the article. Further, data in the first column of Supplementary Table 55 were mistakenly shifted and have been corrected in the file accompanying the HTML version of the article