Definition of danger zones for gas bore holes

Eksploatacja złóż węglowodorów ciekłych i gazowych ma już długoletnią tradycję. Dlatego coraz częściej spotykamy się z negatywnymi skutkami tej działalności przede wszystkim po zaprzestaniu eksploatacji. Do skutków tych zaliczyć można zanieczyszczenia występujące w środowisku naturalnym, w warstwach powierzchniowych, zanieczyszczonych wodach po niekontrolowanych ucieczkach ropy naftowej w procesie eksploatacji lub przeróbki. Problemem są zlikwidowane przed laty odwierty ropne czy gazowe. Z powodu industrializacji terenów, a także długiego czasu, jaki upłynął od zakończenia eksploatacji, ich stan techniczny pozostawia wiele do życzenia. W pracy przedstawiono niektóre zagadnienia dotyczące prognozowania stref zagrożenia związanych z nieczynnymi już kopalniami ropy i gazu oraz podziemnymi magazynami gazu.The exploitation of oil and gas has long lasting tradition. Therefore, more and more often there can be found negative results of this activity, above all so called ecologic loads. To these loads belong: a contamination of natural environment, contamination in superficial layer and contamination of water after uncontrolled leaks of rock oil during the mining or processing. Major problem are long closed gas and oil wells. Because of the industrialization of the areas, and also because of the long time, which passed since the end of exploitations, their condition leaves a lot to desire. This paper presents some of the problems concerning technical forecasting of out of operation oil and gas mines contamination zones

Control of cytokinin and auxin homeostasis in cyanobacteria and algae

Background and Aims The metabolism of cytokinins (CKs) and auxins in vascular plants is relatively well understood, but data concerning their metabolic pathways in non-vascular plants are still rather rare. With the aim of filling this gap, 20 representatives of taxonomically major lineages of cyanobacteria and algae from Cyanophyceae, Xanthophyceae, Eustigmatophyceae, Porphyridiophyceae, Chlorophyceae, Ulvophyceae, Trebouxiophyceae, Zygnematophyceae and Klebsormidiophyceae were analysed for endogenous profiles of CKs and auxins and some of them were used for studies of the metabolic fate of exogenously applied radiolabelled CK, [3H]trans-zeatin (transZ) and auxin ([3H]indole-3-acetic acid (IAA)), and the dynamics of endogenous CK and auxin pools during algal growth and cell division. Methods Quantification of phytohormone levels was performed by high-performance or ultrahigh-performance liquid chromatography–electrospray tandem mass spectrometry (HPLC-MS/MS, UHPLC-MS/MS). The dynamics of exogenously applied [3H]transZ and [3H]IAA in cell cultures were monitored by HPLC with on-line radioactivity detection. Key Results The comprehensive screen of selected cyanobacteria and algae for endogenous CKs revealed a predominance of bioactive and phosphate CK forms while O- and N-glucosides evidently did not contribute greatly to the total CK pool. The abundance of cis-zeatin-type CKs and occurrence of CK 2-methylthio derivatives pointed to the tRNA pathway as a substantial source of CKs. The importance of the tRNA biosynthetic pathway was proved by the detection of tRNA-bound CKs during the course of Scenedesmus obliquus growth. Among auxins, free IAA and its oxidation catabolite 2-oxindole-3-acetic acid represented the prevailing endogenous forms. After treatment with [3H]IAA, IAA-aspartate and indole-3-acetyl-1-glucosyl ester were detected as major auxin metabolites. Moreover, different dynamics of endogenous CKs and auxin profiles during S. obliquus culture clearly demonstrated diverse roles of both phytohormones in algal growth and cell division. Conclusions Our data suggest the existence and functioning of a complex network of metabolic pathways and activity control of CKs and auxins in cyanobacteria and algae that apparently differ from those in vascular plants