Mass budget of the grounded ice in Lambert Glacier-Amery Ice Shelf system

We used remote-sensing and in situ measurements of surface accumulation rate, ice surface velocity, thickness and elevation to evaluate the mass budgets of grounded ice-flow regimes that form the Lambert Glacier-Amery Ice Shelf system. Three distinct drainage regimes are considered: the western and eastern margins of the ice shelf, and the southern grounding line at the major outlet glacier confluence, which can be identified with drainage zones 9, 11 and 10 respectively of Giovinetto and Zwally (2000). Our findings show the entire grounded portion of the basin is approximately in balance, with a mass budget of -4.2_9.8 Gt a-1. Drainages 9, 10 and 11 are within balance to the level of our measurement uncertainty, with mass budgets of -2.5_2.8 Gt a-1, -2.6_7.8 Gt a-1 and 0.9_2.3 Gt a-1, respectively. The region upstream of the Australian Lambert Glacier basin (LGB) traverse has a net mass budget of 4.4_6.3 Gt a-1, while the downstream region has -8.9_9.9 Gt a-1. These results indicate that glacier drainages 9, 10 and 11, upstream and downstream of the Australian LGB traverse, are in balance to within our measurement error

Mass budgets of the Lambert, Mellor and Fisher Glaciers and basal fluxes beneath their flowbands on Amery Ice Shelf

We used in situ measurements and remote-sensing data sets to evaluate the mass budgets of the Lambert, Mellor and Fisher Glaciers and the basal melting and freezing rates beneath their flowbands on the Amery Ice Shelf. Our findings show the Lambert and Mellor Glaciers upstream of the ANARE Lambert Glacier Basin (LGB) traverse may have positive imbalances of 3.9±2.1 Gt a−1 and 2.1±2.4 Gt a−1, respectively, while the Fisher Glacier is approximately in balance. The upstream region as a whole has a positive imbalance of 5.9±4.9 Gt a−1. The three same glaciers downstream of the ANARE LGB traverse line are in negative imbalance, where the whole downstream region has a negative imbalance of −8.5±5.8 Gt a−1. Overall the mass budgets of the Lambert, Mellor, and Fisher Glaciers are close to balance, and the collective three-glacier system is also nearly in balance with a mass budget of −2.6±6.5 Gt a−1. The significant positive imbalances for the interior basin upstream of the ice-movement stations established in the early 1970s (GL line) reported previously are possibly due to an overestimate of the total accumulation and an underestimate of the ice flux through the GL line.The mean melting rate is −23.0±3.5 m ice a−1 near the southern grounding line, which decreases rapidly downstream, and transitions to refreezing at around 300 km from the southern extremity of the Amery Ice Shelf. Freezing rates along the flowbands are around 0.5±0.1 to 1.5±0.2 m ice a−1. The percentage of ice lost from the interior by basal melting beneath the flowbands is about 80%±5%. The total basal melting and refreezing beneath the three flowbands is 50.3±7.5 Gt ice a−1 and 7.0±1.1 Gt ice a−1, respectively. We find a much larger total basal melting and net melting than the results for the whole Amery Ice Shelf derived from previous modeling and oceanographic measurements

Immunodominance analysis of CTL responses to influenza PR8 virus reveals two new dominant and subdominant Kb-restricted epitopes.

Abstract In the present study, a systematic analysis of the influenza (Flu) PR8 determinants recognized by H-2b mice was undertaken. A single Db-restricted immunodominant epitope (NP(366)) was previously known in this system. Twenty-three different Flu PR8-derived peptides that bound either Kb or Db molecules in vitro were identified. Sixteen were immunogenic following peptide immunization of C57BL/6 mice, yet CTL induced by peptide immunization recognized PR8-infected target cells only in the case of the NP(366) and NS2(114) epitopes. Similarly, CTL responses following whole-PR8 virus immunization were detected only for the same two determinants. CTL recognizing these dominant epitopes had high avidity for peptide-pulsed target cells, with 5 to 200 pM of peptide required for 30% specific lysis. In contrast, most (80%) of the remaining epitopes were recognized with lower avidity (30% effective concentration in the range of 0.4-50 nM). Repeated in vitro stimulation of primary CTL cultures revealed one additional Kb-restricted epitope (M1(128)). This peptide bound Kb with high affinity (4.6 nM) and induced CTL that effectively recognized PR8-infected cells. These results suggest that 1) this epitope is produced by natural processing in relatively high amounts and 2) low precursor frequency might be related to the subdominant status of the M1(128) epitope. Taken together, these results illustrate the crucial contributions of MHC-binding capacity, and T cell repertoire availability, to the shaping of the repertoire of CTL specificities for Flu Ag virus.</jats:p

Ex vivo priming for long-term maintenance of antileukemia human cytotoxic T cells suggests a general procedure for adoptive immunotherapy

Adoptive cellular immunotherapy has proven to be a successful approach in preventing and curing cytomegalovirus infection and Epstein-Barr virus-associated lymphomas after bone marrow transplantation. Translation of this approach for preventing leukemia relapse after bone marrow transplantation might require ex vivo priming and long-term maintenance of leukemia blast-specific T cells. To accomplish this goal, procedures were optimized for the in vitro priming of naive CD8 using dendritic cells activated by CD40 ligation, interleukin-12 (IL-12), and IL-7. Using T lymphocytes and dendritic cells obtained from HLA-matched allogeneic bone marrow transplantation donors and leukemia blasts as a source of tumor antigens, anti-acute myeloid leukemia cytotoxic T lymphocytes (CTLs) were induced. In these experiments, it was found that though it is possible to induce CTLs using immature dendritic cells, IL-12, and IL-7, obtaining long-term CTLs requires the presence of CD4 T cells in the priming phase. Using this approach, long-term antileukemia CTL lines could be generated from 4 of 4 bone marrow donors. Because this procedure does not require definition of the target antigen and because it selects responding cells from a virgin T-cell repertoire, its general application is suggested in adoptive immunotherapy and in the definition of tumor rejection antigens

Random association between the peptide repertoire of A2.1 class I and several different DR class II molecules.

Abstract The interaction between synthetic peptides and A2.1 class I MHC molecules has been investigated using an inhibition of Ag presentation assay and unbiased peptide sets derived of either viral or eucaryotic origin. For the various sets, strong binding (defined as significant inhibition at the 30 micrograms/ml level) was detected in 7 to 46% of the peptides tested, with an overall frequency of 26%. A set of self-peptides derived from human beta 2 microglobulin was also included in the study. In this case, strong binding was detected in 3 of 15 peptides (20%), thus formally demonstrating a lack of self-/non-self-discrimination at the level of class I molecules. When the whole A2.1-binding database of 105 peptides thus generated was examined by sequence analysis, a significant correlation was found with a recently proposed A2.1-binding motif, whereas no particular positive or negative association was detected between the capacity to bind A2.1 and three different class II alleles (DR1, DR5, and DR7). Finally, using this approach, several peptides capable of binding both A2.1 and multiple DR alleles have been identified, suggesting possible candidates for development of peptide vaccines eliciting both class I and class II restricted responses.</jats:p