A comparison of the DNA binding and bending capacities and the oligomeric states of the immunity repressors of heteroimmune coliphages P2 and WPhi

Bacteriophages P2 and WΦ are heteroimmune members of the P2-like family of temperate Escherichia coli phages. Temperate phages can grow lytically or form lysogeny after infection. A transcriptional switch that contains two con-vergent promoters, Pe and Pc, and two repressors regulate what life mode to enter. The immunity repressor C is the first gene of the lysogenic operon, and it blocks the early Pe promoter. In this work, some characteristics of the C proteins of P2 and WΦ are compared. An in vivo genetic analysis shows that WΦ C, like P2 C, has a strong dimerization activity in the absence of its DNA target. Both C proteins recognize two directly repeated sequences, termed half-sites and a strong bending is induced in the respective DNA target upon binding. P2 C is unable to bind to one half-site as opposed to WΦ, but both half-sites are required for repression of WΦ Pe. A reduction from three to two helical turns between the centers of the half-sites in WΦ has no significant effect on the capacity to repress Pe. However, the protein–DNA complexes formed differ, as determined by electrophoretic mobility shift experiments. A difference in spontaneous phage production is observed in isogenic lysogens

The POINT-AGAPE survey II: An Unrestricted Search for Microlensing Events towards M31

An automated search is carried out for microlensing events using a catalogue of 44554 variable superpixel lightcurves derived from our three-year monitoring program of M31. Each step of our candidate selection is objective and reproducible by a computer. Our search is unrestricted, in the sense that it has no explicit timescale cut. So, it must overcome the awkward problem of distinguishing long-timescale microlensing events from long-period stellar variables. The basis of the selection algorithm is the fitting of the superpixel lightcurves to two different theoretical models, using variable star and blended microlensing templates. Only if microlensing is preferred is an event retained as a possible candidate. Further cuts are made with regard to (i) sampling, (ii) goodness of fit of the peak to a Paczynski curve, (iii) consistency of the microlensing hypothesis with the absence of a resolved source, (iv) achromaticity, (v) position in the colour-magnitude diagram and (vi) signal-to-noise ratio. Our results are reported in terms of first-level candidates, which are the most trustworthy, and second-level candidates, which are possible microlensing but have lower signal-to-noise and are more questionable. The pipeline leaves just 3 first-level candidates, all of which have very short full-width half-maximum timescale (<5 days) and 3 second-level candidates, which have timescales of 31, 36 and 51 days respectively. We also show 16 third-level lightcurves, as an illustration of the events that just fail the threshold for designation as microlensing candidates. They are almost certainly mainly variable stars. Two of the 3 first-level candidates correspond to known events (PA 00-S3 and PA 00-S4) already reported by the POINT-AGAPE project. The remaining first-level candidate is new.Comment: 22 pages, 18 figures, MNRAS, to appea

The POINT-AGAPE Survey: Comparing Automated Searches of Microlensing Events toward M31

Searching for microlensing in M31 using automated superpixel surveys raises a number of difficulties which are not present in more conventional techniques. Here we focus on the problem that the list of microlensing candidates is sensitive to the selection criteria or "cuts" imposed and some subjectivity is involved in this. Weakening the cuts will generate a longer list of microlensing candidates but with a greater fraction of spurious ones; strengthening the cuts will produce a shorter list but may exclude some genuine events. We illustrate this by comparing three analyses of the same data-set obtained from a 3-year observing run on the INT in La Palma. The results of two of these analyses have been already reported: Belokurov et al. (2005) obtained between 3 and 22 candidates, depending on the strength of their cuts, while Calchi Novati et al. (2005) obtained 6 candidates. The third analysis is presented here for the first time and reports 10 microlensing candidates, 7 of which are new. Only two of the candidates are common to all three analyses. In order to understand why these analyses produce different candidate lists, a comparison is made of the cuts used by the three groups...Comment: 28 pages, 24 figures, 9 table

Efficient red electroluminescence from diketopyrrolopyrrole copolymerised with a polyfluorene

We thank the EC Seventh Framework Programme (FP7/2007-2013) under Grant Agreement No. 212311 (ONE-P), the RTN THREADMILL (EU-Contract No.: MRTN-CT-2006-036040), the ITNs SUPERIOR (PITN-CT-2009-238177), and CONTEST (PITN-CT-2012-317488) as well as the Royal Society, and EPSRC for funding

Classical novae from the POINT-AGAPE microlensing survey of M31 -- I. The nova catalogue

The POINT-AGAPE survey is an optical search for gravitational microlensing events towards the Andromeda Galaxy (M31). As well as microlensing, the survey is sensitive to many different classes of variable stars and transients. Here we describe the automated detection and selection pipeline used to identify M31 classical novae (CNe) and we present the resulting catalogue of 20 CN candidates observed over three seasons. CNe are observed both in the bulge region as well as over a wide area of the M31 disk. Nine of the CNe are caught during the final rise phase and all are well sampled in at least two colours. The excellent light-curve coverage has allowed us to detect and classify CNe over a wide range of speed class, from very fast to very slow. Among the light-curves is a moderately fast CN exhibiting entry into a deep transition minimum, followed by its final decline. We have also observed in detail a very slow CN which faded by only 0.01 mag day$^{-1}$ over a 150 day period. We detect other interesting variable objects, including one of the longest period and most luminous Mira variables. The CN catalogue constitutes a uniquely well-sampled and objectively-selected data set with which to study the statistical properties of classical novae in M31, such as the global nova rate, the reliability of novae as standard-candle distance indicators and the dependence of the nova population on stellar environment. The findings of this statistical study will be reported in a follow-up paper.Comment: 21 pages, 13 figures, re-submitted for publication in MNRAS, typos corrected, references updated, figures 5-9 made cleare

Weak Lensing Mass Reconstruction of the Galaxy Cluster Abell 209

Weak lensing applied to deep optical images of clusters of galaxies provides a powerful tool to reconstruct the distribution of the gravitating mass associated to these structures. We use the shear signal extracted by an analysis of deep exposures of a region centered around the galaxy cluster Abell 209, at redshift z=0.2, to derive both a map of the projected mass distribution and an estimate of the total mass within a characteristic radius. We use a series of deep archival R-band images from CFHT-12k, covering an area of 0.3 deg^2. We determine the shear of background galaxy images using a new implementation of the modified Kaiser-Squires-Broadhurst pipeline for shear determination, which we has been tested against the ``Shear TEsting Program 1 and 2'' simulations. We use mass aperture statistics to produce maps of the 2 dimensional density distribution, and parametric fits using both Navarro-Frenk-White (NFW) and singular-isothermal-sphere profiles to constrain the total mass. The projected mass distribution shows a pronounced asymmetry, with an elongated structure extending from the SE to the NW. This is in general agreement with the optical distribution previously found by other authors. A similar elongation was previously detected in the X-ray emission map, and in the distribution of galaxy colours. The circular NFW mass profile fit gives a total mass of M_{200} = 7.7^{+4.3}_{-2.7} 10^{14} solar masses inside the virial radius r_{200} = 1.8\pm 0.3 Mpc. The weak lensing profile reinforces the evidence for an elongated structure of Abell 209, as previously suggested by studies of the galaxy distribution and velocities.Comment: accepted by A&A, 15 pages, 11 figure

The Anomaly in the Candidate Microlensing Event PA-99-N2

The lightcurve of PA-99-N2, one of the recently announced microlensing candidates towards M31, shows small deviations from the standard Paczynski form. We explore a number of possible explanations, including correlations with the seeing, the parallax effect and a binary lens. We find that the observations are consistent with an unresolved RGB or AGB star in M31 being microlensed by a binary lens. We find that the best fit binary lens mass ratio is about one hundredth, which is one of most extreme values found for a binary lens so far. If both the source and lens lie in the M31 disk, then the standard M31 model predicts the probable mass range of the system to be 0.02-3.6 solar masses (95 % confidence limit). In this scenario, the mass of the secondary component is therefore likely to be below the hydrogen-burning limit. On the other hand, if a compact halo object in M31 is lensing a disk or spheroid source, then the total lens mass is likely to lie between 0.09-32 solar masses, which is consistent with the primary being a stellar remnant and the secondary a low mass star or brown dwarf. The optical depth (or alternatively the differential rate) along the line of sight toward the event indicates that a halo lens is more likely than a stellar lens provided that dark compact objects comprise no less than 15 per cent (or 5 per cent) of haloes.Comment: Latex, 23 pages, 9 figures, in press at The Astrophysical Journa

Search for exoplanets in M31 with pixel-lensing and the PA-99-N2 event revisited

Several exoplanets have been detected towards the Galactic bulge with the microlensing technique. We show that exoplanets in M31 may also be detected with the pixel-lensing method, if telescopes making high cadence observations of an ongoing microlensing event are used. Using a Monte Carlo approach we find that the mean mass for detectable planetary systems is about $2 M_{\rm {J}}$. However, even small mass exoplanets ($M_{\rm P} < 20 M_{\oplus}$) can cause significant deviations, which are observable with large telescopes. We reanalysed the POINT-AGAPE microlensing event PA-99-N2. First, we test the robustness of the binary lens conclusion for this light curve. Second, we show that for such long duration and bright microlensing events, the efficiency for finding planetary-like deviations is strongly enhanced with respect to that evaluated for all planetary detectable events.Comment: 14 pages, 8 figures. Paper presented at the "II Italian-Pakistani Workshop on Relativistic Astrophysics, Pescara, July 8-10, 2009. To be published in a special issue of General Relativity and Gravitation (eds. F. De Paolis, G.F.R. Ellis, A. Qadir and R. Ruffini

Driving factors of aerosol properties over the foothills of central Himalayas based on 8.5 years continuous measurements

This study presents analysis of in situ measurements conducted over the period 2005–2014 in the Indian Himalayas to give a thorough overview of the factors and causes that drive aerosol properties. Aerosol extensive properties (namely, particle number concentration, scattering coefficient, equivalent black carbon, PM2.5, and PM10) have 1.5–2 times higher values in the early to late afternoon than during the night, and a strong seasonality. The interannual variability is ±20% for both PM2.5 and total particle number concentration. Analysis of the data shows statistically significant decreasing trends of −2.3 μg m−3 year−1 and −2.7 μg m−3 year−1 for PM2.5 and PM10, respectively, over the study period. The mountainous terrain site (Mukteshwar, MUK) is primarily under the influence of air from the plains. This is due to convective transport processes that are enhanced by local and mesoscale topography, leading to pronounced valley/mountain winds and consequently to atmospheric boundary layer air lifting from the plains below. The transport from plains is evident in seasonal‐diurnal patterns observed at MUK. The timing of the patterns corresponds with changes in turbulence and water vapor (q). According to our analysis, using these as proxies is a viable method for examining boundary layer influence in the absence of direct atmospheric boundary layer height measurements. Comparing the measurements with climate models shows that even regional climate models have problems capturing the orographic influence accurately at MUK, highlighting the importance of long‐term direct measurements at multiple points to understand aerosol behavior in mountainous areas

Metabolic and physiological adjustment of Suaeda maritima to combined salinity and hypoxia

BACKGROUND AND AIMS: Suaeda maritima is a halophyte commonly found on coastal wetlands in the intertidal zone. Due to its habitat S. maritima has evolved tolerance to high salt concentrations and hypoxic conditions in the soil caused by periodic flooding. In the present work, the adaptive mechanisms of S. maritima to salinity combined with hypoxia were investigated on a physiological and metabolic level. METHODS: To compare the adaptive mechanisms to deficient, optimal and stressful salt concentrations, S. maritima plants were grown in a hydroponic culture under low, medium and high salt concentrations. Additionally, hypoxic conditions were applied to investigate the impact of hypoxia combined with different salt concentrations. A non-targeted metabolic approach was used to clarify the biochemical pathways underlying the metabolic and physiological adaptation mechanisms of S. maritima . KEY RESULTS: Roots exposed to hypoxic conditions showed an increased level of tricarboxylic acid (TCA)-cycle intermediates such as succinate, malate and citrate. During hypoxia, the concentration of free amino acids increased in shoots and roots. Osmoprotectants such as proline and glycine betaine increased in concentrations as the external salinity was increased under hypoxic conditions. CONCLUSIONS: The combination of high salinity and hypoxia caused an ionic imbalance and an increase of metabolites associated with osmotic stress and photorespiration, indicating a severe physiological and metabolic response under these conditions. Disturbed proline degradation in the roots induced an enhanced proline accumulation under hypoxia. The enhanced alanine fermentation combined with a partial flux of the TCA cycle might contribute to the tolerance of S. maritima to hypoxic conditions