Millimetre-VLBI Monitoring of AGN with Sub-milliarcsecond Resolution

Global millimetre VLBI allows detailed studies of the most central jet regions of AGN with unprecedent spatial resolution of a few 100-1000 Schwartzschild radii to be made. Study of these regions will help to answer the question how the highly relativistic AGN jets are launched and collimated. Since the early 1990s, bright mm-sources have been observed with global 3 mm VLBI. Here we present new images from an ongoing systematic analysis of the available observations. In particular, we focus on the structure and structural evolution of the best observed AGN jets, taking 3C 454.3 as a characteristic example. This core-dominated and highly variable quasar shows a complex morphology with individual jet components accelerating superluminally towards the outer structure. We briefly discuss the X-ray properties of 3C 454.3 and present its radio- to X-ray large-scale brightness distribution.Comment: 4 pages, 4 figures, Proceedings of the 7th EVN Symposium held in Toledo, Spain in October 2004, needs evn2004.cl

Modeling of the Super-Eddington Phase for Classical Novae: Five IUE Novae

We present a light curve model for the super-Eddington luminosity phase of five classical novae observed with IUE. Optical and UV light curves are calculated based on the optically thick wind theory with a reduced effective opacity for a porous atmosphere. Fitting a model light curve with the UV 1455 \AA light curve, we determine the white dwarf mass and distance to be (1.3 M_sun, 4.4 kpc) for V693 CrA, (1.05 M_sun, 1.8 kpc) for V1974 Cyg, (0.95 M_sun, 4.1 kpc) for V1668 Cyg, (1.0 M_sun, 2.1 kpc) for V351 Pup, and (1.0 M_sun, 4.3 kpc) for OS And.Comment: 9 pages including 8 figures, to appear in the Astrophysical Journa

Dynamics of Line-Driven Winds from Disks in Cataclysmic Variables. I. Solution Topology and Wind Geometry

We analyze the dynamics of 2-D stationary, line-driven winds from accretion disks in cataclysmic variable stars. The driving force is that of line radiation pressure, in the formalism developed by Castor, Abbott & Klein for O stars. Our main assumption is that wind helical streamlines lie on straight cones. We find that the Euler equation for the disk wind has two eigenvalues, the mass loss rate and the flow tilt angle with the disk. Both are calculated self-consistently. The wind is characterized by two distinct regions, an outer wind launched beyond four white dwarf radii from the rotation axis, and an inner wind launched within this radius. The inner wind is very steep, up to 80 degrees with the disk plane, while the outer wind has a typical tilt of 60 degrees. In both cases the ray dispersion is small. We, therefore, confirm the bi-conical geometry of disk winds as suggested by observations and kinematical modeling. The wind collimation angle appears to be robust and depends only on the disk temperature stratification. The flow critical points lie high above the disk for the inner wind, but close to the disk photosphere for the outer wind. Comparison with existing kinematical and dynamical models is provided. Mass loss rates from the disk as well as wind velocity laws are discussed in a subsequent paper.Comment: 21 pages, 10 Postscript figures; available also from http://www.pa.uky.edu/~shlosman/publ.html. Astrophysical Journal, submitte

de Sitter Supersymmetry Revisited

We present the basic $\mathcal{N} =1$ superconformal field theories in four-dimensional de Sitter space-time, namely the non-abelian super Yang-Mills theory and the chiral multiplet theory with gauge interactions or cubic superpotential. These theories have eight supercharges and are invariant under the full $SO(4,2)$ group of conformal symmetries, which includes the de Sitter isometry group $SO(4,1)$ as a subgroup. The theories are ghost-free and the anti-commutator $\sum_\alpha\{Q_\alpha, Q^{\alpha\dagger}\}$ is positive. SUSY Ward identities uniquely select the Bunch-Davies vacuum state. This vacuum state is invariant under superconformal transformations, despite the fact that de Sitter space has non-zero Hawking temperature. The $\mathcal{N}=1$ theories are classically invariant under the $SU(2,2|1)$ superconformal group, but this symmetry is broken by radiative corrections. However, no such difficulty is expected in the $\mathcal{N}=4$ theory, which is presented in appendix B.Comment: 21 pages, 2 figure

Watching Proteins at Work - Conformational Dynamics of G proteins revealed by Site-directed Spin Labeling EPR Spectroscopy in the framework of Integrative Structural Approaches

Comprehensive understanding of the function of proteins requires knowledge about the structural and dynamics properties of these molecules, especially when they work as enzymes or interact with other proteins. In general, the key step for any process in life is a binding event. Either a substrate binds to an enzyme, a protein-ligand interaction, or two or more proteins form a complex involving protein-protein interactions, and in some cases, like for the GTP-hydrolyzing proteins investigated in this work, a protein-protein interaction, namely (homo-)dimerization, is required to form a functional enzyme and protein-protein and protein-ligand interactions influence each other. The results presented here have been obtained using site-directed spin labeled protein variants and EPR spectroscopy to obtain information about the structure and dynamics of five different G proteins that dimerize (Ras, MnmE, CtRoco, hLRRK2) or form larger oligomeric assemblies (hGBP1). The information about spin label side chain dynamics from cw EPR experiments and inter-spin distances mainly from pulsed EPR, specifically DEER, experiments obtained in these studies has been combined with other biophysical data and computational methods, foremost MD simulations, in an integrative approach. In integrative structural biology approaches, data from multiple methods is combined to obtain structural models and reveal information about the dynamic behavior of large and often complex macromolecular assemblies. The studies presented in this work all use integrative approaches to different extents to obtain insights into the structural and dynamic properties of different G proteins that form larger complexes by dimerization. The results obtained are discussed in the present context

The crystal structure of the C-terminal domain of the salmonella enterica pduo protein: An old fold with a new heme-binding mode

The two-domain protein PduO, involved in 1,2-propanediol utilization in the pathogenic Gram-negative bacterium Salmonella enterica is an ATP:Cob(I)alamin adenosyltransferase, but this is a function of the N-terminal domain alone. The role of its C-terminal domain (PduOC) is, however, unknown. In this study, comparative growth assays with a set of Salmonella mutant strains showed that this domain is necessary for effective in vivo catabolism of 1,2-propanediol. It was also shown that isolated, recombinantly-expressed PduOC binds heme in vivo. The structure of PduOC co-crystallized with heme was solved (1.9 \uc5 resolution) showing an octameric assembly with four heme moieities. The four heme groups are highly solvent-exposed and the heme iron is hexa-coordinated with bis-His ligation by histidines from different monomers. Static light scattering confirmed the octameric assembly in solution, but a mutation of the heme-coordinating histidine caused dissociation into dimers. Isothermal titration calorimetry using the PduOC apoprotein showed strong heme binding (Kd = 1.6 7 10 127 M). Biochemical experiments showed that the absence of the C-terminal domain in PduO did not affect adenosyltransferase activity in vitro. The evidence suggests that PduOC:heme plays an important role in the set of cobalamin transformations required for effective catabolism of 1,2-propanediol. Salmonella PduO is one of the rare proteins which binds the redox-active metabolites heme and cobalamin, and the heme-binding mode of the C-terminal domain differs from that in other members of this protein family