Utilización de inmunoreacciones para la detección de Scrapie en Óbex de ovinos provenientes de la XII Región de Chile

Scrapie is an neurodegenerative and infectious disease that affects sheep and goats. The purpose of this study is to compare both tecniques, ELISA and IHC, to determine the application factibility giving the conditions of the laboratory in the country. To develop this assay, fourty obex samples were taken from sheep of the XII Region of Chile and were processed for both techniques ELISA and IHC. The results showed 100% of the samples negative for Scrapie, using both diagnostic methods. Based in obtained and previously reported results, ELISA technique can be recommended to use in massive screening, since yields reliable and rapid test results and IHC is a confirmatory technique. Both techniques are necessary in the creation of current epidemiological surveillance programs in Chile. ELISA and IHC are easily performed given the current laboratories conditions of the Chilean country.    El Scrapie es una enfermedad infecciosa neurodegenerativa que afecta a ovinos y caprinos. Dada la importancia que tiene esta enfermedad es que se desarrolló este estudio enmarcado en la comparación de la técnica de ELISA y la de inmunohistoquímica en el diagnóstico de Scrapie, para determinar la factibilidad de aplicarlas en las condiciones de laboratorio país. Para ello se procesaron 40 muestras de óbex de ovinos en duplicado, provenientes de la XII Región de Chile, las cuales fueron sometidas paralelamente a la técnica de ELISA e IHQ. Como resultados se obtuvo un 100% de muestras negativas a Scrapie, sometidas a ambos métodos diagnósticos. Con los valores informados previamente y los obtenidos en esta investigación, es posible recomendar el ELISA como una técnica utilizable en muestreos masivos, ya que aporta resultados confiables de manera más rápida. La IHQ corresponde a una técnica confirmatoria y que ambas, son necesarias en la creación de programas de vigilancia epidemiológica activa en Chile, siendo factibles de realizar en las actuales condiciones de laboratorio país.  

Streamlining the Change-Over Protocol for the RPA Mission Intelligence Coordinator by way of Situation Awareness Oriented Design and Discrete Event Simulation

Incredible loiter times coupled with the ability to make extremely detailed collections at significant stand-off distances with a relatively expendable platform has made demand for, and diversity of, Remotely Piloted Aircraft (RPA) operations grow at voracious rates. Conversely, financial resources are becoming increasingly constrained. As such, innovators are looking to maximize the effectiveness of existing personnel and assets by considering concepts such as simultaneous Multiple Aircraft Control (MAC) by a single aircrew. Research has identified procedural inefficiencies in current operations as well as substantial impediments to MAC implementation, including dynamic task saturation and communication challenges. An identified inefficiency afflicting both current operations and the feasibility of MAC is the time required to transfer operational situation awareness at shift change -- dubbed change-over. The present research employed synergistic application of Cognitive Task Analyses, Situation Awareness Oriented Design, and Monte Carlo simulation to inform the development of a highly efficient user-centered process for the Mission Intelligence Coordinator -- the RPA aircrew\u27s situation awareness linchpin. Discrete-event simulations were performed on existing and proposed protocols. These analyses indicate that the proposed protocol could require as little as one-third the time required by the current method. It is proposed that such an improvement could significantly increase current RPA mission-readiness as well as diminish a known obstacle to MAC implementation

Relationship Between Osteoporosis and Marginal Bone Loss in Osseointegrated Implants: A 2-Year Retrospective Study

Background: Fitting implants in osteoporotic patients has raditionally been controversial, and there is little scientific evidence relating osteoporosis to marginal bone loss (MBL). The aims of this study are as follows: 1) to evaluate the possibility of a correlation between osteoporosis, as measured by the mandibular cortical index (MCI), and MBL and 2) to assess how various systemic diseases, periodontitis, and placement of implants in regenerated bone are correlated with MBL and MCI. Methods: This retrospective study examines 212 implants inserted in 67 patients. To take a possible cluster failure into account, an implant for each patient was selected (n = 67 implants). MBL was assessed. Osteoporosis was evaluated using the MCI. Both MBL and MCI were assessed from panoramic radiographs. x2 test was performed (Haberman post hoc test). Significance was P <0.05. Results: When the total sample implant (N = 212) was evaluated, a significant association was found between the presence of osteoporosis and MCI (P <0.001) and between the presence of diabetes mellitus and MCI (P <0.01). Significant associations were also found between MBL and placement of implants in regenerated sites (P <0.001) and between MBL and a previous history of periodontitis (P <0.05). When the sample is evaluated only in selected implants (one per patient, n = 67), significant differences appear to relate only to the MBL with the placement of implants in regenerated bone sites (P <0.001). Conclusions: Osteoporosis (as evaluated by MCI) does not pose a risk for the development of greater MBL. Parameters adversely affecting the development of increased MBL are a previous history of periodontitis and especially the placement of implants at sites of bone regeneration

Buenas prácticas de transferencia del conocimiento en la Universidad de Córdoba

La iniciativa consiste en proporcionar la materia prima. El proyecto, que se ha realizado con cerdos y ha tenido una duración de tres años, demuestra que la administración de Cardiotrofina-1 en el transplante hepático incrementa la supervivencia del animal, mejora su función cardiaca, respiratoria y renal, y también consigue reducir el daño hepatocelecuar y el estrés oxidativo y nitrosativo en el injerto

Movilidad cotidiana hacia el Campus Resistencia: el caso de los estudiantes de arquitectura de la FAU-UNNE

University students correspond to an important sector of thepopulation with particular travel patterns that combine study,work and leisure activities. Understanding their ways of gettingaround can help universities and local administrations promotepolicies that encourage forms of mobility that guarantee accessibility to the campus. This work seeks to characterize themobility of students towards the Resistencia Campus of theNational University of the Northeast. The data analyzed comesfrom a mobility survey carried out on advanced students ofthe Architecture career, where they were consulted about themeans used, reasons and main conditions of the trip.Los estudiantes universitarios corresponden a un importante sector de la población con patrones de desplazamientoparticulares que combinan actividadesde estudio, trabajo y ocio. Entender susformas de movilizarse puede ayudar alas universidades y administracioneslocales a promover políticas que fomenten formas de movilidad que garanticenla accesibilidad al campus. Este trabajobusca caracterizar la movilidad de losestudiantes hacia el Campus Resistencia de la Universidad Nacional del Nordeste. Los datos analizados proceden deuna encuesta de movilidad realizada aestudiantes avanzados de la carrera deArquitectura, donde se consultó sobrelos medios utilizados, motivos y principales condicionantes del viaje

Multi-dimensional entanglement generation with multi-core optical fibers

Trends in photonic quantum information follow closely the technical progress in classical optics and telecommunications. In this regard, advances in multiplexing optical communications channels have also been pursued for the generation of multi-dimensional quantum states (qudits), since their use is advantageous for several quantum information tasks. One current path leading in this direction is through the use of space-division multiplexing multi-core optical fibers, which provides a new platform for efficiently controlling path-encoded qudit states. Here we report on a parametric down-conversion source of entangled qudits that is fully based on (and therefore compatible with) state-of-the-art multi-core fiber technology. The source design uses modern multi-core fiber beam splitters to prepare the pump laser beam as well as measure the generated entangled state, achieving high spectral brightness while providing a stable architecture. In addition, it can be readily used with any core geometry, which is crucial since widespread standards for multi-core fibers in telecommunications have yet to be established. Our source represents an important step towards the compatibility of quantum communications with the next-generation optical networks.Comment: 9 pages, 7 figure

Quinolone Resistance Reversion by Targeting the SOS Response

Suppression of the SOS response has been postulated as a therapeutic strategy for potentiating antimicrobial agents. We aimed to evaluate the impact of its suppression on reversing resistance using a model of isogenic strains of Escherichia coli representing multiple levels of quinolone resistance. E. coli mutants exhibiting a spectrum of SOS activity were constructed from isogenic strains carrying quinolone resistance mechanisms with susceptible and resistant phenotypes. Changes in susceptibility were evaluated by static (MICs) and dynamic (killing curves or flow cytometry) methodologies. A peritoneal sepsis murine model was used to evaluate in vivo impact. Suppression of the SOS response was capable of resensitizing mutant strains with genes encoding three or four different resistance mechanisms (up to 15-fold reductions in MICs). Killing curve assays showed a clear disadvantage for survival (Δlog10 CFU per milliliter [CFU/ml] of 8 log units after 24 h), and the in vivo efficacy of ciprofloxacin was significantly enhanced (Δlog10 CFU/g of 1.76 log units) in resistant strains with a suppressed SOS response. This effect was evident even after short periods (60 min) of exposure. Suppression of the SOS response reverses antimicrobial resistance across a range of E. coli phenotypes from reduced susceptibility to highly resistant, playing a significant role in increasing the in vivo efficacy

Meta-tirosina. Un poderoso agente anti-metastásico sin efectos tóxicos colaterales detectables

La resistencia concomitante antitumoral (RC) es el fenómeno según el cual un individuo portador de tumor inhibe el crecimiento de implantes tumorales secundarios y metástasis. Si bien desde hace tiempo se sabe que la RC inducida por tumores inmunogénicos de pequeño tamaño es generada por mecanismos inmunológicos dependientes de células T, por otro lado, la manifestación más universal de la RC, generada tanto por tumores inmunogénicos como no-inmunogénicos de gran tamaño, había sido asociada con un (unos) factor sérico antitumoral cuya naturaleza permaneció elusiva por años. En un trabajo reciente, nuestro grupo de trabajo identificó este factor como la mezcla equi-molar de meta-tirosina y orto-tirosina, dos isómeros de tirosina que no están presentes en proteínas normales y que demostraron ser responsables del 90% y 10%, respectivamente, de la actividad antitumoral total del suero. En este trabajo, continuamos nuestras investigaciones demostrando que la administración periódica de meta-tirosina reducía drásticamente el número de metástasis pulmonares y hepáticas en ratones portadores de dos tumores murinos altamente metastásicos y disminuía dramáticamente la mortandad (de 100% a 25%) de ratones con metástasis ya establecidas al momento de la extirpación quirúrgica del tumor. Estos efectos anti-metastásicos se lograron aun con muy bajas concentraciones de meta-tirosina y sin efectos tóxicos perceptibles, lo que sugiere que su uso puede ayudar a diseñar nuevas y menos nocivas estrategias para el tratamiento del cáncer, especialmente aquellas destinadas a controlar el crecimiento metastásico, que es el problema más grave en la enfermedad oncológica.Concomitant tumor resistance (CR) is a phenomenon in which a tumor-bearing host is resistant to the growth of secondary tumor implants and metastasis. While former studies have indicated that T-cell dependent processes mediate CR in hosts bearing immunogenic small tumors, the most universal manifestation of CR induced by immunogenic and non-immunogenic large tumors had been associated with an antitumor serum factor that remained an enigma for many years. In a recent paper, we identified that elusive factor(s) as an equi-molar mixture of meta-tyrosine and ortho-tyrosine, two isomers of tyrosine that are not present in normal proteins and that proved to be responsible for 90% and 10%, respectively, of the total serum anti-tumor activity. In this work, we have extended our previous findings demonstrating that a periodic intravenous administrationof meta-tyrosine induced a dramatic reduction of lung and hepatic metastases generated in mice bearing two different metastatic murine tumors and decreased the rate of death from 100% up to 25% in tumor-excised mice that already exhibited established metastases at the time of surgery. These anti-metastatic effects were achieved even at very low concentrations and without displaying any detectable toxic-side effects, suggesting that the use of meta-tyrosine may help to develop new and less harmful means of managing malignant diseases, especiallythose aimed to control the growth of metastases that is the most serious problem in cancer pathology.Fil: Machuca, Damián Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Chiarella, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Montagna, Daniela Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Dran, Graciela Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Meiss, Roberto P.. Academia Nacional de Medicina de Buenos Aires; ArgentinaFil: Ruggiero, Raul Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentin