Molecular gas freeze-out in the pre-stellar core L1689B

C17O (J=2-1) observations have been carried out towards the pre-stellar core L1689B. By comparing the relative strengths of the hyperfine components of this line, the emission is shown to be optically thin. This allows accurate CO column densities to be determined and, for reference, this calculation is described in detail. The hydrogen column densities that these measurements imply are substantially smaller than those calculated from SCUBA dust emission data. Furthermore, the C17O column densities are approximately constant across L1689B whereas the SCUBA column densities are peaked towards the centre. The most likely explanation is that CO is depleted from the central regions of L1689B. Simple models of pre-stellar cores with an inner depleted region are compared with the results. This enables the magnitude of the CO depletion to be quantified and also allows the spatial extent of the freeze-out to be firmly established. We estimate that within about 5000 AU of the centre of L1689B, over 90% of the CO has frozen onto grains. This level of depletion can only be achieved after a duration that is at least comparable to the free-fall timescale.Comment: MNRAS letters. 5 pages, 5 figure

Human breast cancer bone metastasis in vitro and in vivo: a novel 3D model system for studies of tumour cell-bone cell interactions.

Bone is established as the preferred site of breast cancer metastasis. However, the precise mechanisms responsible for this preference remain unidentified. In order to improve outcome for patients with advanced breast cancer and skeletal involvement, we need to better understand how this process is initiated and regulated. As bone metastasis cannot be easily studied in patients, researchers have to date mainly relied on in vivo xenograft models. A major limitation of these is that they do not contain a human bone microenvironment, increasingly considered to be an important component of metastases. In order to address this shortcoming, we have developed a novel humanised bone model, where 1 × 10(5) luciferase-expressing MDA-MB-231 or T47D human breast tumour cells are seeded on viable human subchaodral bone discs in vitro. These discs contain functional osteoclasts 2-weeks after in vitro culture and positive staining for calcine 1-week after culture demonstrating active bone resorption/formation. In vitro inoculation of MDA-MB-231 or T47D cells colonised human bone cores and remained viable for <4 weeks, however, use of matrigel to enhance adhesion or a moving platform to increase diffusion of nutrients provided no additional advantage. Following colonisation by the tumour cells, bone discs pre-seeded with MDA-MB-231 cells were implanted subcutaneously into NOD SCID mice, and tumour growth monitored using in vivo imaging for up to 6 weeks. Tumour growth progressed in human bone discs in 80 % of the animals mimicking the later stages of human bone metastasis. Immunohistochemical and PCR analysis revealed that growing MDA-MB-231 cells in human bone resulted in these cells acquiring a molecular phenotype previously associated with breast cancer bone metastases. MDA-MB-231 cells grown in human bone discs showed increased expression of IL-1B, HRAS and MMP9 and decreased expression of S100A4, whereas, DKK2 and FN1 were unaltered compared with the same cells grown in mammary fat pads of mice not implanted with human bone discs

Molecular line contamination in the SCUBA-2 450 {\mu}m and 850 {\mu}m continuum data

Observations of the dust emission using millimetre/submillimetre bolometer arrays can be contaminated by molecular line flux, such as flux from 12CO. As the brightest molecular line in the submillimetre, it is important to quantify the contribution of CO flux to the dust continuum bands. Conversion factors were used to convert molecular line integrated intensities to flux detected by bolometer arrays in mJy per beam. These factors were calculated for 12CO line integrated intensities to the SCUBA-2 850 {\mu}m and 450 {\mu}m bands. The conversion factors were then applied to HARP 12CO 3-2 maps of NGC 1333 in the Perseus complex and NGC 2071 and NGC 2024 in the Orion B molecular cloud complex to quantify the respective 12CO flux contribution to the 850 {\mu}m dust continuum emission. Sources with high molecular line contamination were analysed in further detail for molecular outflows and heating by nearby stars to determine the cause of the 12CO contribution. The majority of sources had a 12CO 3-2 flux contribution under 20 per cent. However, in regions of molecular outflows, the 12CO can dominate the source dust continuum (up to 79 per cent contamination) with 12CO fluxes reaching \sim 68 mJy per beam.Comment: Accepted 2012 April 19 for publication in MNRAS. 21 pages, 13 figures, 3 table

The relationship between the prestellar core mass function and the stellar initial mass function

Stars form from dense molecular cores, and the mass function of these cores (the CMF) is often found to be similar to the form of the stellar initial mass function (IMF). This suggests that the form of the IMF is the result of the form of the CMF. However, most stars are thought to form in binary and multiple systems, therefore the relationship between the IMF and the CMF cannot be trivial. We test two star formation scenarios - one in which all stars form as binary or triple systems, and one in which low-mass stars form in a predominantly single mode. We show that from a log-normal CMF, similar to those observed, and expected on theoretical grounds, the model in which all stars form as multiples gives a better fit to the IMF.Comment: 7 pages, 3 figures, A&A in pres

Profiling filaments: comparing near-infrared extinction and submillimetre data in TMC-1

Interstellar filaments are an important part of star formation. To understand the structure of filaments, cross-section profiles are often fitted with Plummer profiles. This profiling is often done with submm studies, such as Herschel. It would be convenient if filament properties could also be studied using groundbased NIR data. We compare the filament profiles obtained by NIR extinction and submm observations to find out if reliable profiles can be derived using NIR data. We use J-, H-, and K-band data of a filament north of TMC-1 to derive an extinction map from colour excesses of background stars. We compare the Plummer profiles obtained from extinction maps with Herschel dust emission maps. We present 2 methods to estimate profiles from NIR: Plummer profile fits to median Av of stars or directly to the Av of individual stars. We compare the methods by simulations. In simulations extinction maps and the new methods give correct results to within ~10-20 for modest densities. Direct fit to data on individual stars gives more accurate results than extinction map, and can work in higher density. In profile fits to real observations, values of Plummer parameters are generally similar to within a factor of ~2. Although parameter values can vary significantly, estimates of filament mass usually remain accurate to within some tens of per cent. Our results for TMC-1 are in agreement with earlier results. High resolution NIR data give more details, but 2MASS data can be used to estimate profiles. NIR extinction can be used as an alternative to submm observations to profile filaments. Direct fits of stars can also be a valuable tool. Plummer profile parameters are not always well constrained, and caution should be taken when making fits. In the evaluation of Plummer parameters, one can use the independence of dust emission and NIR data and the difference in the shapes of the confidence regions.Comment: accepted to Astronomy & Astrophysics; abstract has been shortened for astrop

Measurements of cosmic-ray energy spectra with the 2nd CREAM flight

During its second Antarctic flight, the CREAM (Cosmic Ray Energetics And Mass) balloon experiment collected data for 28 days, measuring the charge and the energy of cosmic rays (CR) with a redundant system of particle identification and an imaging thin ionization calorimeter. Preliminary direct measurements of the absolute intensities of individual CR nuclei are reported in the elemental range from carbon to iron at very high energy.Comment: 4 pages, 3 figures, presented at XV International Symposium on Very High Energy Cosmic Ray Interactions (ISVHECRI 2008

A VLA search for young protostars embedded in dense cores

Four dense cores, L1582A, L1689A, B133 and B68, classified as prestellar in terms of the absence of detectable NIR emission, are observed at radio wavelengths to investigate whether they nurture very young protostars. No definite young protostars were discovered in any of the four cores observed. A few radio sources were discovered close to the observed cores, but these are most likely extragalactic sources or YSOs unrelated to the cores observed. In L1582A we discovered a weak radio source near the centre of the core with radio characteristics and offset from the peak of the submillimeter emission similar to that of the newly discovered protostar in the core L1014, indicating a possible protostellar nature for this source. This needs to be confirmed with near- and/or mid-infrared observations (e.g. with Spitzer). Hence based on the current observations we are unable to confirm unequivocally that L1582A is starless. In L1689A a possible 4.5-sigma radio source was discovered at the centre of the core, but needs to be confirmed with future observations. In B133 a weak radio source, possibly a protostar, was discovered at the edge of the core on a local peak of the core submm emission, but no source was detected at the centre of the core. Thus, B133 is probably starless, but may have a protostar at its edge. In B68 no radio sources were discovered inside or at the edge of the core, and thus B68 is indeed starless. Four more radio sources with spectral indices characteristic of young protostars were discovered outside the cores but within the extended clouds in which these cores reside. Conclusions: We conclude that the number of cores misclassified as prestellar is probably very small and does not significantly alter the estimated lifetime of the prestellar phase.Comment: Accepted by A&

Energy spectra of cosmic-ray nuclei at high energies

We present new measurements of the energy spectra of cosmic-ray (CR) nuclei from the second flight of the balloon-borne experiment Cosmic Ray Energetics And Mass (CREAM). The instrument included different particle detectors to provide redundant charge identification and measure the energy of CRs up to several hundred TeV. The measured individual energy spectra of C, O, Ne, Mg, Si, and Fe are presented up to $\sim 10^{14}$ eV. The spectral shape looks nearly the same for these primary elements and it can be fitted to an $E^{-2.66 \pm 0.04}$ power law in energy. Moreover, a new measurement of the absolute intensity of nitrogen in the 100-800 GeV/$n$ energy range with smaller errors than previous observations, clearly indicates a hardening of the spectrum at high energy. The relative abundance of N/O at the top of the atmosphere is measured to be $0.080 \pm 0.025$(stat.)$\pm 0.025$(sys.) at $\sim$800 GeV/$n$, in good agreement with a recent result from the first CREAM flight.Comment: 32 pages, 10 figures. Accepted for publication in Astrophysical Journa