Fish stock assessment of the northern New Caledonian lagoons : 3-Fishing pressure, potential yields and impact on management options

Cette étude évalue le potentiel d’exploitation des poissons démersaux récifo-lagonaires d’intérêt commercial dans les lagons de la Province Nord de la Nouvelle-Calédonie. La pression de pêche est estimée d’après les relevés des pêcheurs professionnels et le budget de consommation des ménages. Les captures totales ont été estimées à 1 326 tonnes en 1995, dont 94 % sont représentés par la pêche de poissons consommés sur place. Les captures sont effectuées sur les côtes ouest et est, et sont très faibles dans le lagon nord (Bélep). Compte tenu de cette pression de pêche d’une part, et des stocks d’autre part, la prise maximale soutenue annuelle a été estimée à 12 600 t, soit environ 10 % du stock total (138 300 t). Elle est principalement constituée par des espèces de cinq familles : les Lethrinidés, Acanthuridés, Scaridés, Serranidés et Lutjanidés. Comparés à la pression de pêche sur l’ensemble des lagons, les stocks semblent loin d’être menacés dans leur ensemble. Il serait donc possible d’augmenter sensiblement l’effort de pêche. Cependant, à une échelle géographique plus fine, certains secteurs communaux (Koné et Népoui sur la côte ouest), certains biotopes récifaux (les abords récifaux), ou certains groupes d’espèces (poissons de ligne) semblent supporter déjà une activité conséquente. Des recommandations sont formulées, notamment la mise en place d’un suivi de la ressource des espèces cibles et la réorientation de l’effort de pêche en direction d’autres groupes d’espèces et d’autres biotopes grâce à une diversification des techniques de captures

THE IMPACT OF DIETARY PROTEIN OR AMINO ACID SUPPLEMENTATION ON MUSCLE MASS AND STRENGTH IN ELDERLY PEOPLE: INDIVIDUAL PARTICIPANT DATA AND META-ANALYSIS OF RCT’S

Objectives Increasing protein or amino acid intake has been promoted as a promising strategy to increase muscle mass and strength in elderly people, however, long-term intervention studies show inconsistent findings. Therefore, we aim to determine the impact of protein or amino acid supplementation compared to placebo on muscle mass and strength in older adults by combining the results from published trials in a metaanalysis and pooled individual participant data analysis. Design We searched Medline and Cochrane databases and performed a meta-analysis on eight available trials on the effect of protein or amino acid supplementation on muscle mass and strength in older adults. Furthermore, we pooled individual data of six of these randomized double-blind placebo-controlled trials. The main outcomes were change in lean body mass and change in muscle strength for both the meta-analysis and the pooled analysis. Results The meta-analysis of eight studies (n=557) showed no significant positive effects of protein or amino acid supplementation on lean body mass (mean difference: 0.014 kg: 95% CI -0.152; 0.18), leg press strength (mean difference: 2.26 kg: 95% CI -0.56; 5.08), leg extension strength (mean difference: 0.75 kg: 95% CI: -1.96, 3.47) or handgrip strength (mean difference: -0.002 kg: 95% CI -0.182; 0.179). Likewise, the pooled analysis showed no significant difference between protein and placebo treatment on lean body mass (n=412: p=0.78), leg press strength (n=121: p=0.50), leg extension strength (n=121: p=0.16) and handgrip strength (n=318: p=0.37). Conclusions There is currently no evidence to suggest that protein or amino acid supplementation without concomitant nutritional or exercise interventions increases muscle mass or strength in predominantly healthy elderly people

Sequence-specific antimicrobials using efficiently delivered RNA-guided nucleases

Current antibiotics tend to be broad spectrum, leading to indiscriminate killing of commensal bacteria and accelerated evolution of drug resistance. Here, we use CRISPR-Cas technology to create antimicrobials whose spectrum of activity is chosen by design. RNA-guided nucleases (RGNs) targeting specific DNA sequences are delivered efficiently to microbial populations using bacteriophage or bacteria carrying plasmids transmissible by conjugation. The DNA targets of RGNs can be undesirable genes or polymorphisms, including antibiotic resistance and virulence determinants in carbapenem-resistant Enterobacteriaceae and enterohemorrhagic Escherichia coli. Delivery of RGNs significantly improves survival in a Galleria mellonella infection model. We also show that RGNs enable modulation of complex bacterial populations by selective knockdown of targeted strains based on genetic signatures. RGNs constitute a class of highly discriminatory, customizable antimicrobials that enact selective pressure at the DNA level to reduce the prevalence of undesired genes, minimize off-target effects and enable programmable remodeling of microbiota.National Institutes of Health (U.S.) (New Innovator Award 1DP2OD008435)National Centers for Systems Biology (U.S.) (Grant 1P50GM098792)United States. Defense Threat Reduction Agency (HDTRA1-14-1-0007)Massachusetts Institute of Technology. Institute for Soldier Nanotechnologies (W911NF13D0001)National Institute of General Medical Sciences (U.S.) (Interdepartmental Biotechnology Training Program 5T32 GM008334)Fonds de la recherche en sante du Quebec (Master's Training Award

Parasympathetic Activity and Blood Catecholamine Responses Following a Single Partial-Body Cryostimulation and a Whole-Body Cryostimulation

The aim of this study was to compare the effects of a single whole-body cryostimulation (WBC) and a partial-body cryostimulation (PBC) (i.e., not exposing the head to cold) on indices of parasympathetic activity and blood catecholamines. Two groups of 15 participants were assigned either to a 3-min WBC or PBC session, while 10 participants constituted a control group (CON) not receiving any cryostimulation. Changes in thermal, physiological and subjective variables were recorded before and during the 20-min after each cryostimulation. According to a qualitative statistical analysis, an almost certain decrease in skin temperature was reported for all body regions immediately after the WBC (mean decrease±90% CL, -13.7±0.7°C) and PBC (-8.3±0.3°C), which persisted up to 20-min after the session. The tympanic temperature almost certainly decreased only after the WBC session (-0.32±0.04°C). Systolic and diastolic blood pressures were very likely increased after the WBC session, whereas these changes were trivial in the other groups. In addition, heart rate almost certainly decreased after PBC (-10.9%) and WBC (-15.2%) sessions, in a likely greater proportion for WBC compared to PBC. Resting vagal-related heart rate variability indices (the root-mean square difference of successive normal R-R intervals, RMSSD, and high frequency band, HF) were very likely increased after PBC (RMSSD: +54.4%, HF: +138%) and WBC (RMSSD: +85.2%, HF: +632%) sessions without any marked difference between groups. Plasma norepinephrine concentrations were likely to very likely increased after PBC (+57.4%) and WBC (+76.2%), respectively. Finally, cold and comfort sensations were almost certainly altered after WBC and PBC, sensation of discomfort being likely more pronounced after WBC than PBC. Both acute cryostimulation techniques effectively stimulated the autonomic nervous system (ANS), with a predominance of parasympathetic tone activation. The results of this study also suggest that a whole-body cold exposure induced a larger stimulation of the ANS compared to partial-body cold exposure

Sex Specific Differences in the Response to Short Term Muscle Disuse in Middle Aged Adults

Research on short term muscle disuse has demonstrated the numerous adverse effects it causes on skeletal muscle. These include reductions in function and mass, as well as insulin sensitivity, mitochondrial function and muscle protein synthesis. However, little is known about the sex specific differences in how males and females respond to muscle disuse. PURPOSE: To investigate the sex specific differences in how males and females respond to short term disuse. METHODS: Sixteen healthy, middle-aged adults (8 males; 57.9±1.8 yr; BMI 29.9±1.2 kg·m-2; 8 females; 55.1±1.6 yr; BMI 28.9±1.0 kg·m-2) underwent 7 days of unilateral leg immobilization via unilateral lower limb suspension (ULLS). Muscle biopsies from the vastus lateralis were collected prior to and following immobilization (in both control [CON] and immobilized [IMM] legs). We applied the stable isotope tracer deuterium oxide to measure muscle protein synthesis (MPS), immunoblotting to assess mTOR signaling and assessed leg strength changes via unilateral leg extension 1 repetition maximum (1RM). We hypothesized that males would experience greater reductions in MPS, mitochondrial function and leg strength compared to females. RESULTS: MPS was 36.5±10.2 % and 16.5±15.6 % lower in the IMM leg compared to the CON leg in females (P\u3c0.05) and males (P\u3e0.05) respectively during immobilization. There was no difference in MPS in the IMM leg between males and females (P\u3e0.05) during immobilization. Leg extension 1RM declined to the same extent in females 17.1±2.1 % and males 24.2±2.4 % after 7 days of IMM (both P\u3c0.05). Markers of mTOR signaling were unaltered by IMM in both males and females (P\u3e0.05). CONCLUSION: Middle aged males and females experience the same reduction in leg strength and muscle protein synthesis after short term disuse

Erythropoietin Does Not Enhance Skeletal Muscle Protein Synthesis Following Exercise in Young and Older Adults

PURPOSE: Erythropoietin (EPO) is a renal cytokine that is primarily involved in hematopoiesis while also playing a role in non-hematopoietic tissues expressing the EPO-receptor (EPOR). The EPOR is present in human skeletal muscle. In mouse skeletal muscle, EPO stimulation can activate the AKT serine/threonine kinase 1 (AKT) signaling pathway, the main positive regulator of muscle protein synthesis. We hypothesized that a single intravenous EPO injection combined with acute resistance exercise would have a synergistic effect on skeletal muscle protein synthesis via activation of the AKT pathway. METHODS: Ten young (24.2 ± 0.9 years) and 10 older (66.6 ± 1.1 years) healthy subjects received a primed, constant infusion of [ring-13C(6)] L-phenylalanine and a single injection of 10,000 IU epoetin-beta or placebo in a double-blind randomized, cross-over design. 2 h after the injection, the subjects completed an acute bout of leg extension resistance exercise to stimulate skeletal muscle protein synthesis. RESULTS: Significant interaction effects in the phosphorylation levels of the members of the AKT signaling pathway indicated a differential activation of protein synthesis signaling in older subjects when compared to young subjects. However, EPO offered no synergistic effect on vastus lateralis mixed muscle protein synthesis rate in young or older subjects. CONCLUSIONS: Despite its ability to activate the AKT pathway in skeletal muscle, an acute EPO injection had no additive or synergistic effect on the exercise-induced activation of muscle protein synthesis or muscle protein synthesis signaling pathways

Synthetic biology to access and expand nature's chemical diversity

Bacterial genomes encode the biosynthetic potential to produce hundreds of thousands of complex molecules with diverse applications, from medicine to agriculture and materials. Accessing these natural products promises to reinvigorate drug discovery pipelines and provide novel routes to synthesize complex chemicals. The pathways leading to the production of these molecules often comprise dozens of genes spanning large areas of the genome and are controlled by complex regulatory networks with some of the most interesting molecules being produced by non-model organisms. In this Review, we discuss how advances in synthetic biology — including novel DNA construction technologies, the use of genetic parts for the precise control of expression and for synthetic regulatory circuits — and multiplexed genome engineering can be used to optimize the design and synthesis of pathways that produce natural products

Diurnal cortisol patterns are associated with physical performance in the Caerphilly Prospective Study

Background Cross-sectional studies have suggested that elevated cortisol is associated with worse physical performance, a surrogate of ageing. We examined the relationship between repeat cortisol measures over 20 years and physical performance in later life

Palladium-Catalyzed Carboetherification and Carboamination Reactions of Γ-Hydroxy- and Γ-Aminoalkenes for the Synthesis of Tetrahydrofurans and Pyrrolidines

Substituted tetrahydrofuran and pyrrolidine moieties are displayed in a wide range of interesting biologically active molecules. The Pd-catalyzed carboetherification or carboamination of Γ-hydroxy and Γ-aminoalkenes is a powerful tool for the construction of these heterocycles, as it is convergent and can allow access to a variety of analogs from a single Γ-hydroxy- or Γ-aminoalkene starting material. This microreview describes the current state of this field. (© Wiley-VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2007)Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/55970/1/571_ftp.pd