Squeezed Fermions at Relativistic Heavy Ion Colliders

Large back-to-back correlations of observable fermion -- anti-fermion pairs are predicted to appear, if the mass of the fermions is modified in a thermalized medium. The back-to-back correlations of protons and anti-protons are experimentally observable in ultra-relativistic heavy ion collisions, similarly to the Andreev reflection of electrons off the boundary of a superconductor. While quantum statistics suppresses the probability of observing pairs of fermions with nearby momenta, the fermionic back-to-back correlations are positive and of similar strength to bosonic back-to-back correlations.Comment: LaTeX, ReVTeX 12 pages, uses epsf.sty, 2 eps figures, improved presentatio

Back-to-Back Correlations for Finite Expanding Fireballs

Back-to-Back Correlations of particle-antiparticle pairs are related to the in-medium mass-modification and squeezing of the quanta involved. They are predicted to appear when hot and dense hadronic matter is formed in high energy nucleus-nucleus collisions. The survival and magnitude of the Back-to-Back Correlations of boson-antiboson pairs generated by in-medium mass modifications are studied here in the case of a thermalized, finite-sized, spherically symmetric expanding medium. We show that the BBC signal indeed survives the finite-time emission, as well as the expansion and flow effects, with sufficient intensity to be observed at RHIC.Comment: 24 pages, 4 figure

Determination of Ganciclovir in Plasma of Newborns with Congenital CMV Infection

Infections caused by human cytomegalovirus are important causes of fetal and neonatal morbidity and mortality. Ganciclovir ([(9-(1,3-dihydroxy-2- propoxymethyl) guanine, GCV) is a synthetic acyclic nucleoside which has shown activity against Cytomegalovirus. GCV treatment has been associated with serious toxic hematological effects such as neutropenia and leukopenia, thus drug monitoring is needed, especially in the case of newborns. The aim of the work is to develop and validate an HPLC method for the quantification of GCV in plasm

Comparisons of Protein and Peptide Complexity in Poneroid and Formicoid Ant Venoms

© 2016 American Chemical Society. Animal venom peptides are currently being developed as novel drugs and bioinsecticides. Because ants use venoms for defense and predation, venomous ants represent an untapped source of potential bioactive toxins. This study compared the protein and peptide components of the poneroid ants Neoponera commutata, Neoponera apicalis, and Odontomachus hastatus and the formicoid ants Ectatomma tuberculatum, Ectatomma brunneum, and Myrmecia gulosa. 1D and 2D PAGE revealed venom proteins in the mass range 250 kDa. NanoLC-ESI-QTOF MS/MS analysis of tryptic peptides revealed the presence of common venom proteins and also many undescribed proteins. RP-HPLC separation followed by MALDI-TOF MS of the venom peptides also revealed considerable heterogeneity. It was found that the venoms contained between 144 and 1032 peptides with 5-95% of peptides in the ranges 1-4 and 1-8 kDa for poneroid and formicoid ants, respectively. By employing the reducing MALDI matrix 1,5-diaminonapthalene, up to 28 disulfide-bonded peptides were also identified in each of the venoms. In particular, the mass range of peptides from poneroid ants is lower than peptides from other venoms, indicating possible novel structures and pharmacologies. These results indicate that ant venoms represent an enormous, untapped source of novel therapeutic and bioinsecticide leads

TLR3 engagement induces IRF-3-dependent apoptosis in androgen-sensitive prostate cancer cells and inhibits tumour growth in vivo

Toll-like receptors (TLRs) are a family of highly conserved transmembrane proteins expressed in epithelial and immune cells that recognize pathogen associated molecular patterns. Besides their role in immune response against infections, numerous studies have shown an important role of different TLRs in cancer, indicating these receptors as potential targets for cancer therapy. We previously demonstrated that the activation of TLR3 by the synthetic double-stranded RNA analogue poly I:C induces apoptosis of androgen-sensitive prostate cancer (PCa) LNCaP cells and, much less efficiently, of the more aggressive PC3 cell line. Therefore, in this study we selected LNCaP cells to investigate the mechanism of TLR3-mediated apoptosis and the in vivo efficacy of poly I:C-based therapy. We show that interferon regulatory factor-3 (IRF-3) signalling plays an essential role in TLR3-mediated apoptosis in LNCaP cells through the activation of the intrinsic and extrinsic apoptotic pathways. Interestingly, hardly any apoptosis was induced by poly I:C in normal prostate epithelial cells RWPE-1. We also demonstrate for the first time the direct anticancer effect of poly I:C as a single therapeutic agent in a well-established human androgen-sensitive PCa xenograft model, by showing that tumour growth is highly impaired in poly I:C-treated immunodeficient mice. Immunohistochemical analysis of PCa xenografts highlights the antitumour role of poly I:C in vivo both on cancer cells and, indirectly, on endothelial cells. Notably, we show the presence of TLR3 and IRF-3 in both human normal and PCa clinical samples, potentially envisaging poly I:C-based therapy for PCa

Stem-like and highly invasive prostate cancer cells expressing CD44v8-10 marker originate from CD44-negative cells

In human prostate cancer (PCa), the neuroendocrine cells, expressing the prostate cancer stem cell (CSC) marker CD44, may be resistant to androgen ablation and promote tumor recurrence. During the study of heterogeneity of the highly aggressive neuroendocrine PCa cell lines PC3 and DU-145, we isolated and expanded in vitro a minor subpopulation of very small cells lacking CD44 (CD44neg). Unexpectedly, these sorted CD44neg cells rapidly and spontaneously converted to a stable CD44high phenotype specifically expressing the CD44v8-10 isoform which the sorted CD44high subpopulation failed to express. Surprisingly and potentially interesting, in these cells expression of CD44v8-10 was found to be induced in stem cell medium. CD44 variant isoforms are known to be more expressed in CSC and metastatic cells than CD44 standard isoform. In agreement, functional analysis of the two sorted and cultured subpopulations has shown that the CD44v8-10pos PC3 cells, resulting from the conversion of the CD44neg subpopulation, were more invasive in vitro and had a higher clonogenic potential than the sorted CD44high cells, in that they produced mainly holoclones, known to be enriched in stem-like cells. Of interest, the CD44v8-10 is more expressed in human PCa biopsies than in normal gland. The discovery of CD44v8-10pos cells with stem-like and invasive features, derived from a minoritarian CD44neg cell population in PCa, alerts on the high plasticity of stem-like markers and urges for prudency on the approaches to targeting the putative CSC

Bose-Einstein Correlations for Three-Dimensionally Expanding, Cylindrically Symmetric, Finite Systems

The parameters of the Bose-Einstein correlation function may obey an {\it $M_t$-scaling}, as observed in $S + Pb$ and $Pb + Pb$ reactions at CERN SPS. This $M_t$-scaling implies that the Bose-Einstein correlation functions view only a small part of the big and expanding system. The full sizes of the expanding system at the last interaction are shown to be measurable with the help the invariant momentum distribution of the emitted particles. A vanishing duration parameter can also be generated in the considered model-class with a specific $M_t$ dependence.Comment: 35 pages, ReVTeX, LaTeX, no figures, discussion extende

An accurate spline polynomial cubature formula for double integration with logarithmic singularity

The paper studied the integration of logarithmic singularity problem J(ӯ) = ∫∫Δζ(ӯ)log|ӯ - ӯ 0∗|dA, where ӯ=(α,β), y0=(α0,β0) the domain Δ is rectangle Δ = [r1, r2] × [r3, r4], the arbitrary point ӯ ϵ Δ and the fixed point ӯ0 ϵ Δ. The given density function ζ(ӯ), is smooth on the rectangular domain Δ and is in the functions class C2,τ (Δ). Cubature formula (CF) for double integration with logarithmic singularities (LS) on a rectangle Δ is constructed by applying type (0, 2) modified spline function DΓ(P). The results obtained by testing the density functions ζ(ӯ) as linear and absolute value functions shows that the constructed CF is highly accurate

Thermal Infrared Radiometric Calibration of the Entire Landsat 4, 5, and 7 Archive (1982-2010)

Landsat's continuing record of the thermal state of the earth's surface represents the only long term (1982 to the present) global record with spatial scales appropriate for human scale studies (i.e., tens of meters). Temperature drives many of the physical and biological processes that impact the global and local environment. As our knowledge of, and interest in, the role of temperature on these processes have grown, the value of Landsat data to monitor trends and process has also grown. The value of the Landsat thermal data archive will continue to grow as we develop more effective ways to study the long term processes and trends affecting the planet. However, in order to take proper advantage of the thermal data, we need to be able to convert the data to surface temperatures. A critical step in this process is to have the entire archive completely and consistently calibrated into absolute radiance so that it can be atmospherically compensated to surface leaving radiance and then to surface radiometric temperature. This paper addresses the methods and procedures that have been used to perform the radiometric calibration of the earliest sizable thermal data set in the archive (Landsat 4 data). The completion of this effort along with the updated calibration of the earlier (1985 1999) Landsat 5 data, also reported here, concludes a comprehensive calibration of the Landsat thermal archive of data from 1982 to the presen