Wirkung des in Staehlen enthaltenen Siliciums beim Feuerverzinken von Stahlteilen Gebrauchseigenschaften und Betriebsverhalten. Abschlussbericht. Vertragsdauer: 1.12.1975-30.11.1979

TIB Hannover: Z 2297 (7402) / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekSIGLEDEGerman

Bone marrow cytological evaluation in dogs with chronic kidney disease

Since anemia is indicated as an important compromising factor for the quality of lifeof dogs with chronic kidney disease (CKD), bone marrow cytological analysis mayprovide more information on the hematological profile these dogs and, therefore,allow clinicians to not only choose the most adequate treatment but also monitor theresponse to therapy. The aim of this study was to investigate the feasibility withsternal bone marrow puncture in chronic kidney disease (CKD) using only localanesthesia and check if the cytological analysis is helpful to determine thehematological status. We found that erythroid hypoplasia occurred only in terminalCKD patients, and that the bone marrows of dogs with CKD stages 2 and 3 werequantitatively similar to those of elderly dogs. All dogs tolerated the bone marrowpuncture using only local anesthesia with lidocaine and bone marrow cytologicalevaluation may be a useful tool for hematopoietic evaluation of anemic dogs withCKD.Sendo a anemia apontada como um importante fator comprometedor da qualidade de vidados cães portadores de doença renal crônica (DRC), a análise citológica da medulaóssea poderia fornecer mais informações acerca do perfil hematológico desses animaie, portanto, possibilitar ao clínico não somente optar por um tratamento maiadequado, mas também monitorar a resposta à terapia. Diante disso, objetivou-se comeste estudo avaliar a praticabilidade e a aceitação do procedimento de coleta demedula óssea do osso esterno de cães com doença renal crônica (DRC) utilizandosomente anestesia local, bem como verificar se os resultados fornecidos na análisecitológica fazem-se úteis à composição do quadro hematológico desses pacientes.Observou-se que a manifestação de hipoplasia da série eritroide só ocorreu nopacientes em estádio terminal da DRC, e que as medulas ósseas dos DRC em estádio 2 e3 assemelharam-se quantitativamente à medula de animais idosos. Os cães de todos dogrupos toleraram bem a punção do osso esterno somente com uso de lidocaína, podendoser esta, portanto, uma ferramenta útil na complementação da abordagem hematopoiéticade cães anêmicos com DRC.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Estadual Paulista (UNESP), JaboticabalUniversidade Estadual Paulista (UNESP), JaboticabalFAPESP: 2008/56616-1FAPESP: 2009/52230-

Analysis of chemokine receptor CCR7 expression on porcine blood T lymphocytes using a CCL19-Fc fusion protein

The chemokine receptor CCR7 has been a useful marker for the characterization of human and mouse T cell subsets. We have produced the porcine CCR7 ligand CCL19 fused to the human IgG1 Fc fragment, and used it to analyse CCR7 expression in swine. CCL19-Fc bound to and induced the migration of cells expressing porcine CCR7 but not of untransfected cells, corroborating its specificity. On blood lymphocytes, CCL19-Fc labelled the majority of CD4+ T cells expressing the 2E3 marker, associated with a naïve phenotype, whereas the 2E3- cells were mostly negative. Among CD8+ T cells CCL19-Fc labelled two subsets one, CD8βhi CD11alo CD45RA+, perforin-/lo , which produced low amounts of IFN-γ after stimulation, which might correspond to naïve cells; and a second small population of CD8βlo cells which expressed high levels of CD11a, and were mostly CD45RA-, a phenotype which resembles that of human central memory T cells. © 2012 Elsevier Ltd