How the other half lives: CRISPR-Cas's influence on bacteriophages

CRISPR-Cas is a genetic adaptive immune system unique to prokaryotic cells used to combat phage and plasmid threats. The host cell adapts by incorporating DNA sequences from invading phages or plasmids into its CRISPR locus as spacers. These spacers are expressed as mobile surveillance RNAs that direct CRISPR-associated (Cas) proteins to protect against subsequent attack by the same phages or plasmids. The threat from mobile genetic elements inevitably shapes the CRISPR loci of archaea and bacteria, and simultaneously the CRISPR-Cas immune system drives evolution of these invaders. Here we highlight our recent work, as well as that of others, that seeks to understand phage mechanisms of CRISPR-Cas evasion and conditions for population coexistence of phages with CRISPR-protected prokaryotes.Comment: 24 pages, 8 figure

Methodology for dimensioning the socio-economic impact of power-to-gas technologies in a circular economy scenario

Innovative and sustainable energy technologies are needed in the transition of energy toward a circular economy. Because of the use of renewable energy and carbon utilization, power-to-gas could be a cutting-edge technology that supports the circular model in future sustainable energy markets. However, this technology faces new technical and socio-economic challenges. The use of power-to-gas is limited because of barriers that limit the mobilization of investment capital. In addition, social and economic impacts on the territories in which these facilities are located are under study. In this context, the aims of this paper are: (i) To explore the determinants and barriers for power-to-gas technology to enhance the understanding of investment in innovative energy technologies; and (ii) to support effective policymaking and energy companies’ decision-making processes. This study defines and measures, from a circular economy perspective, the main impacts of the deployment of this technology on a territory in terms of volume of investment, employment generation, and CO2 capture. The study also provides a simplified methodology to contribute to the analysis of the use of power-to-gas. Finally, it improves the knowledge of the socio-economic impact of this cutting-edge technology for the transition of energy to a zero-emission scenario

Novel interactions of transglutaminase-2 with heparan sulphate proteoglycans: reflection on physiological implications

This mini-review brings together information from publications and recent conference proceedings that have shed light on the biological interaction between transglutaminase-2 and heparan sulphate proteoglycans. We subsequently draw hypothesis of possible implications in the wound healing process. There is a substantial overlap in the action of transglutaminase-2 and the heparan sulphate proteoglycan syndecan-4 in normal and abnormal wound repair. Our latest findings have identified syndecan-4 as a possible binding and signalling partner of fibronectinbound TG2 and support the idea that transglutaminase-2 and syndecan-4 acts in synergy

Biabduction (and related problems) in array separation logic

We investigate array separation logic (\mathsf {ASL}), a variant of symbolic-heap separation logic in which the data structures are either pointers or arrays, i.e., contiguous blocks of memory. This logic provides a language for compositional memory safety proofs of array programs. We focus on the biabduction problem for this logic, which has been established as the key to automatic specification inference at the industrial scale. We present an \mathsf {NP} decision procedure for biabduction in \mathsf {ASL}, and we also show that the problem of finding a consistent solution is \mathsf {NP}-hard. Along the way, we study satisfiability and entailment in \mathsf {ASL}, giving decision procedures and complexity bounds for both problems. We show satisfiability to be \mathsf {NP}-complete, and entailment to be decidable with high complexity. The surprising fact that biabduction is simpler than entailment is due to the fact that, as we show, the element of choice over biabduction solutions enables us to dramatically reduce the search space

Creating the cultures of the future: cultural strategy, policy and institutions in Gramsci. Part three: Is there a theory of cultural policy in Gramsci’s prison notebooks?

In this article, I argue that Gramsci’s prison notes on questions of cultural strategy, policy and institutions, which have so far been largely overlooked by scholars, provide further analytical insights to those offered by his more general concepts. Together they enrich the theoretical underpinnings for critical frameworks of analysis as well as for radical practices of cultural strategy, cultural policy-making and cultural organisation. On the basis of a detailed analysis of these notes, I then answer the question of whether they amount to a theory of cultural policy

The Impact of Eco-Innovation on Performance Through the Measurement of Financial Resources and Green Patents

The main objective of this article is to contribute empirically to the understanding of the impact that eco-innovation has on firms’ financial performance within the framework of the resources-based view. Specifically, eco-innovation is measured by using eco-innovative activities and financial resources applied to eco-innovation to argue that the identification and measurement of certain resources of firms allow companies that are particularly active in investing in eco-innovation to be more competitive. Furthermore, the analysis attempts to ascertain whether firms that own green patents and other characteristics exhibit different level of financial performance than firms without registered green patents. The empirical partial least squares structural equation modeling results indicate a positive relationship between the investment of resources and the financial performance of eco-innovative firms. The effects of involving managers in eco-innovative processes as an environmental capability of firms are also tested

Analysis of energy poverty intensity from the perspective of the regional administration: Empirical evidence from households in southern Europe

The current economic situation has increased the number of households in Europe experiencing restrictions and/or limitations of affordability of energy services, demonstrating the urgent need to intervene in those extreme cases in which households suffer the daily consequences of what is internationally defined as energy poverty. In such a context, this paper presents the results obtained in a case study characterising a sample of 615 households with demonstrated energy poverty in the region of Aragón (Spain). In parallel, the intensity of energy poverty in the studied cases is examined by measuring the percentage of energy expenditures with respect to income in the households that suffer it, and a descriptive analysis of the main determinants of energy poverty in the homes studied is presented as well as the policy implication at regional level

ESCOGITARE Project: Towards a Grid-based e-Science Infrastructure for the Italian Agricultural Research Council (CRA)

Advances in agricultural research have resulted in massive increases in the volume and variety of available data. In particular, the complexity of biological information necessitates powerful bioinformatics computational resources both at the level of storage and analysis of data. Moreover, the heterogeneous bioinformatics-related knowledge of scientists involved in agricultural research makes it fundamental to provide an in-situ training program dedicated to the most utilized computational tools. All these challenges are being tackled by the ”ESCOGITARE” project which should provide an e-Science based, virtual lab structure for plant and animal genomics to help in the understanding of important traits such as yield, stress resistance and quality. The recently founded Italian Agricultural Research Council can be considered as a good example of application of such a collaborative research approach due to the number of the belonging institutes and to the wide range of competences characterizing each of them