Why Don't Farmers Adopt Precision Farming Technologies in Cotton Production?

We used the 2009 Southern Cotton Precision Farming Survey data collected from farmers in twelve U.S. states (Alabama, Arkansas, Florida, Georgia, Louisiana, Missouri, Mississippi, North Carolina, South Carolina, Tennessee, Texas, and Virginia) to understand why farmers do not adopt seemingly profitable precision farming technology. Farmers provided cost, time constraint, satisfaction with the current practice and other as reasons for not adopting precision farming technology. Results from a multinomial logit regression model indicated that manure application on field, more formal education, larger farm size, participation in conservation easement or agricultural easement generally decreases the probability of nonadoption of precision agriculture in cotton production.precision agriculture, technology adoption, multinomial logit, Crop Production/Industries, Farm Management, Research and Development/Tech Change/Emerging Technologies, C25, Q16,

Seed germination and seedling allogamy in Rosmarinus officinalis: the costs of inbreeding

1) Self-pollination by geitonogamy is likely in self-compatible plants that simultaneously expose large numbers of flowers to pollinators. However, the progeny of these plants is often highly allogamous. Although mechanisms to increase cross-pollination have been identified and studied, their relative importance has rarely been addressed simultaneously in plant populations. (2) We used Rosmarinus officinalis to explore the factors that influence the probability of self-fertilization due to geitonogamy or that purge its consequences, focusing on their effect on seed germination and allogamy rate. For doing this, we experimentally tested the effects of geitonogamy on the proportion of filled seeds and how it influences germination rates. Then during two field seasons, we studied how life-history and flowering traits of individuals influence seed germination and allogamy rates of their progeny in wild populations at the extremes of the altitudinal range. The traits considered were plant size, population density, duration of the flowering season, number of open flowers, flowering synchrony among individuals within populations, and the proportion of male-sterile flowers. (3) We found that most seeds obtained experimentally from self-pollinations were apparently healthy but in fact empty, and that the presence of filled seeds drove the differences in germination rates between self- and cross-pollination experiments. Plants from wild populations consistently showed low germination rates and high rates of allogamy as determined with microsatellites. Germination rates related positively to the length of the flowering season, flowering synchrony and the rate of male-sterile flowers whereas the rate of allogamous seedlings was positively related only to the rate of male-sterile flowers. (4) Rosemary plants purge most of the inbreeding caused by its pollination system by aborting seeds. This study showed that the rates of seed germination and of the resulting allogamy are a function of a complex combination of factors that vary in space and time. Male sterility of flowers, length of the flowering season and flowering synchrony of individuals within populations all favor high rates of cross-pollination, therefore increasing germination and allogamy rates. These flowering traits appear to be highly plastic and respond to local and seasonal environmental conditions

Profiling embryonic stem cell differentiation by MALDI TOF mass spectrometry:development of a reproducible and robust sample preparation workflow

MALDI TOF mass spectrometry (MS) is widely used to characterise and biotype bacterial samples, but a complementary method for profiling of mammalian cells is still underdeveloped. Current approaches vary dramatically in their sample preparation methods and are not suitable for high-throughput studies. In this work, we present a universal workflow for mammalian cell MALDI TOF MS analysis and apply it to distinguish ground-state naïve and differentiating mouse embryonic stem cells (mESCs), which can be used as a model for drug discovery. We employed a systematic approach testing many parameters to evaluate how efficiently and reproducibly each method extracted unique mass features from four different human cell lines. These data enabled us to develop a unique mammalian cell MALDI TOF workflow involving a freeze-thaw cycle, methanol fixing and a CHCA matrix to generate spectra that robustly phenotype different cell lines and are highly reproducible in peak identification across replicate spectra. We applied our optimised workflow to distinguish naïve and differentiating populations using multivariate analysis and reproducibly identify unique features. We were also able to demonstrate the compatibility of our optimised method for current automated liquid handling technologies. Consequently, our MALDI TOF MS profiling method enables identification of unique features and robust phenotyping of mESC differentiation in under 1 hour from culture to analysis, which is significantly faster and cheaper when compared with conventional methods such as qPCR. This method has the potential to be automated and can in the future be applied to profile other cell types and expanded towards cellular MALDI TOF MS screening assays.</p

Retos y nuevas perspectivas en la revegetación de sistemas forestales

Entre los días 21 y 23 de noviembre de 2007 tuvo lugar la I Reunión del Grupo de Trabajo de Repoblaciones Forestales de la Sociedad Española de Ciencias Forestales (SECF) y el grupo de trabajo de Restauración Ecológica de la Asociación Española de Ecología Terrestre, y IV Reunión del Grupo de Trabajo de Repoblaciones Forestales de la SECF en las instalaciones del Rectorado de la Universidad de Alcalá. A la reunión asistieron 74 participantes, y se presentaron un total de 39 comunicaciones orales, 18 paneles y 8 ponencias invitadas. Además, se presentó el libro “Calidad de Planta Forestal para la Restauración en Ambientes Mediterráneos” y se organizaron dos mesas redondas, una sobre “Necesidades reales de investigación en calidad de planta y cultivo de planta forestal en vivero” (Tabla 1) y otra sobre “Necesidades reales de la investigación en reforestación” (Tabla 2, 3). Una densa agenda para alcanzar los principales objetivos de la reunión, la presentación de investigación de calidad en el ámbito de la revegetación de sistemas forestales, y el intercambio de experiencias e inquietudes entre los diversos colectivos implicados en la misma. Pero ¿qué imagen podemos extraer del estado actual de nuestros conocimientos en esta área a partir de las comunicaciones presentadas

La metodología de aprendizaje basado en proyectos (ABP) aplicada a asignaturas de ciencia de los materiales en ingeniería en la red IdM@ti

En este trabajo se va a presentar una experiencia docente llevada a cabo de forma coordinada entre profesorado de la red de innovación docente en ciencia de materiales IdM@ti. Se trata por tanto de una experiencia interuniversitaria que se ha desarrollado entre cuatro universidades públicas españolas de manera simultánea. Concretamente, el presente trabajo muestra la implementación y el desarrollo de la aplicación de la metodología de aprendizaje basado en proyectos en asignaturas de grados de ingeniería en el ámbito de ciencia de los materiales e ingeniería metalúrgica. Se presentan las experiencias llevadas a cabo durante los últimos cuatro años, mostrando los puntos fuertes y débiles de esta metodología, así como la problemática asociada a la implementación de la misma en asignaturas de curso general de grado. Se presentarán también los resultados obtenidos, así como las principales conclusiones a las que ha llegado el equipo de trabajo de la red IdM@ti

Prediction of near field overpressure from quarry blasting

This paper investigates the propagation of airblast or pressure waves in air produced by bench blasting (i.e. detonation of the explosive in a row of blastholes, breaking the burden of rock towards the free vertical face of the block). Peak overpressure is calculated as a function of blasting parameters (explosive mass per delay and velocity at which the detonation sequence proceeds along the bench) and the polar coordinates of the position of interest (distance to the source and azimuth with respect to the free face). The model has been fitted to empirical data using linear least squares. The data set is composed of 122 airblast records monitored at distances less than 400 m in 41 production blasts carried out in two quarries. The model is statistically significant and has a determination coefficient of 0.87. The formula is validated from 12 airblast measurements gathered in five additional blasts

Aging enhances contraction to thromboxane A2 in aorta from female senescence mice

The time-course for aging-associated effects on vascular reactivity to U46619, a stable analogue of thromboxane A2 (TXA2), was studied in aorta from female senescence-accelerated mice-prone (SAMP8), a murine model of accelerated senescence. SAMP8 and senescence-accelerated mice-resistant (SAMR1) were divided into three groups: 3-, 6- and 10-month-old. Contractile curves to U46619 (10−9 to 10−6 M) were performed in aortic rings in the absence or in the presence of nitric oxide synthase (NOS) inhibitor NG-nitro-L-arginine methyl ester (L-NAME; 10−4 M) and/or cyclooxygenase (COX) inhibitor indomethacin (10−5 M). Protein and gene expression for COX-1 and COX-2 were determined by immunofluorescence and real-time PCR, respectively. Maximal contraction to U46619 was markedly higher in SAMP8 at all ages. In SAMR1, increases were seen at 10 months, while SAMP8 displays augmented contraction at 6 months, which was further increased at 10 months. L-NAME enhanced U46619 contractions in both 6-month-old groups, although the increase was higher on vessels from SAMR1 at this age. Indomethacin equally increased U46619 contractions in both 3-month-old groups, suggesting the production of vasodilator prostaglandin in young animals. In contrast, at 6 and 10 months indomethacin decreased U46619 contractions in both groups, indicating an aging-associated swap to a release of contractile prostanoids in aorta. In conclusion, aging enhances contractile responses to TXA2 in aorta from female mice by a mechanism involving a decrease of NO production and increased action of contractile prostanoids. This process occurs earlier in SAMP8 mice, establishing these mice as good model to study cardiovascular aging in a convenient and standard time-course

Laser Calibration System for Time of Flight Scintillator Arrays

A laser calibration system was developed for monitoring and calibrating time of flight (TOF) scintillating detector arrays. The system includes setups for both small- and large-scale scintillator arrays. Following test-bench characterization, the laser system was recently commissioned in experimental Hall B at the Thomas Jefferson National Accelerator Facility for use on the new Backward Angle Neutron Detector (BAND) scintillator array. The system successfully provided time walk corrections, absolute time calibration, and TOF drift correction for the scintillators in BAND. This showcases the general applicability of the system for use on high-precision TOF detectors.Comment: 11 pages, 11 figure