Perfluoro Alkyl Hypofluorites and Peroxides Revisited

A more convenient synthesis of the perfluoro alkyl hypofluorite (F3C)3COF as well as the hitherto unknown (C2F5)(F3C)2COF compound is reported. Both hypofluorites can be prepared by use of the corresponding tertiary alcohols RFOH and elemental fluorine in the presence of CsF. An appropriate access to these highly reactive hypofluorites is crucial. The hypofluorites are then transferred into their corresponding perfluoro bisalkyl peroxides RFOORF [RF=(F3C)3C, (C2F5)(F3C)2C] by treatment with partially fluorinated silver wool. NMR, gas‐phase infrared, and solid‐state Raman spectra of the perfluoro bisalkyl peroxides are presented and their chemical properties are discussed

Complete genome sequence of Syntrophobacter fumaroxidans strain (MPOB(T)).

Syntrophobacter fumaroxidans strain MPOB(T) is the best-studied species of the genus Syntrophobacter. The species is of interest because of its anaerobic syntrophic lifestyle, its involvement in the conversion of propionate to acetate, H2 and CO2 during the overall degradation of organic matter, and its release of products that serve as substrates for other microorganisms. The strain is able to ferment fumarate in pure culture to CO2 and succinate, and is also able to grow as a sulfate reducer with propionate as an electron donor. This is the first complete genome sequence of a member of the genus Syntrophobacter and a member genus in the family Syntrophobacteraceae. Here we describe the features of this organism, together with the complete genome sequence and annotation. The 4,990,251 bp long genome with its 4,098 protein-coding and 81 RNA genes is a part of the Microbial Genome Program (MGP) and the Genomes to Life (GTL) Program project

Bacteriophage DNA glucosylation impairs target DNA binding by type I and II but not by type V CRISPR-Cas effector complexes

Prokaryotes encode various host defense systems that provide protection against mobile genetic elements. Restriction-modification (R-M) and CRISPR-Cas systems mediate host defense by sequence specific targeting of invasive DNA. T-even bacteriophages employ covalent modifications of nucleobases to avoid binding and therefore cleavage of their DNA by restriction endonucleases. Here, we describe that DNA glucosylation of bacteriophage genomes affects interference of some but not all CRISPR-Cas systems. We show that glucosyl modification of 5-hydroxymethylated cytosines in the DNA of bacteriophage T4 interferes with type I-E and type II-A CRISPR-Cas systems by lowering the affinity of the Cascade and Cas9-crRNA complexes for their target DNA. On the contrary, the type V-A nuclease Cas12a (also known as Cpf1) is not impaired in binding and cleavage of glucosylated target DNA, likely due to a more open structural architecture of the protein. Our results suggest that CRISPR-Cas systems have contributed to the selective pressure on phages to develop more generic solutions to escape sequence specific host defense systems

Mindful “Servants:” Reflective Practices in a High School Service-Learning Classroom

Increasingly, public school teachers, administrators, and students are turning towards including reflective practices, such as “mindfulness” activities, in their daily teaching and learning experiences. This article shows the importance of how practitioners and students use and experience these practices, especially in a service-learning program aimed at pushing students to change habits and ways of thinking. This qualitative study explores reflective techniques applied to a high school service-learning course that was created and maintained to push students out of their “comfort zones” and further into community exploration and self-reflection. Results of the study conclude that reflective activities were effective in class and service contexts, supported personal growth and connection, and that students experienced “buy-in,” embracing weekly journal assignments, one-one-one-conversations, and meditation. Keywords: Mindfulness, service-learning, reflection, meditation, adolescents, high schoo

(Re)connect social and environmental responsibility to learners’ living environments: Curriculum challenges and possible solutions for teaching-learning in Life Orientation

An analysis of the National Curriculum Statement indicates a strong focus on the promotion of knowledge in local context, whilst being sensitive to global imperatives. This implies that learning experiences must reflect local realities first; a call which compels teachers to adapt their teaching with the purpose to make learning relevant and meaningful for the learner. It is therefore an appropriate time to ask the key question: Do Life Orientation teachers (re)connect their teaching-learning on social and environmental responsibility with learners’ living environments to adhere to curriculum requirements of relevance and meaningfulness? The qualitative data obtained from 13 structured classroom observations, with specific reference to the topic social and environmental responsibility, revealed that Life Orientation teachers experienced challenges. Based on these qualitative findings the researchers propose place-based education as an appropriate teaching-learning strategy to (re)connect social and environmental responsibility with the learner’s living environment

Tetrabenzoporphyrin and -mono-, - Cis -di- and tetrabenzotriazaporphyrin derivatives: Electrochemical and spectroscopic implications of meso CH Group replacement with nitrogen

Nonperipherally hexyl-substituted metal-free tetrabenzoporphyrin (2H-TBP, 1a) tetrabenzomonoazaporphyrin (2H-TBMAP, 2a), tetrabenzo-cis-diazaporphyrin (2H-TBDAP, 3a), tetrabenzotriazaporphyrin (2H-TBTAP, 4a), and phthalocyanine (2H-Pc, 5a), as well as their copper complexes (1b-5b), were synthesized. As the number of meso nitrogen atoms increases from zero to four, Îmax of the Q-band absorption peak becomes red-shifted by almost 100 nm, and extinction coefficients increased at least threefold. Simultaneously the blue-shifted Soret (UV) band substantially decreased in intensity. These changes were related to the relative electron-density of each macrocycle expressed as the group electronegativity sum of all meso N and CH atom groups, âχR. X-ray photoelectron spectroscopy differentiated between the three different types of macrocyclic nitrogen atoms (the Ninner, (NH)inner, and Nmeso) in the metal-free complexes. Binding energies of the Nmeso and Ninner,Cu atoms in copper chelates could not be resolved. Copper insertion lowered especially the cathodic redox potentials, while all four observed redox processes occurred at larger potentials as the number of meso nitrogens increased. Computational chemical methods using density functional theory confirmed 1b to exhibit a Cu(II) reduction prior to ring-based reductions, while for 2b, Cu(II) reduction is the first reductive step only if the nonperipheral substituents are hydrogen. When they are methyl groups, it is the second reduction process; when they are ethyl, propyl, or hexyl, it becomes the third reductive process. Spectro-electrochemical measurements showed redox processes were associated with a substantial change in intensity of at least two main absorbances (the Q and Soret bands) in the UV spectra of these compounds

The COVID-19 pandemic and blood utilisation in South Africa

Background. The COVID‐19 pandemic, first reported in December 2019, affected every sector of every country worldwide. Health services were the first to experience the direct impact. Blood services were advised to decrease blood utilisation in anticipation of reduced blood collections. The South African National Blood Services (SANBS) saw a substantial decline in blood demand following the institution of strict national lockdown measures, but the impact of the epidemic and various mitigation strategies remained unknown. Objective. This retrospective study reviews red blood cell (RBC) utilisation during a 4‐year period from 2019 to 2022 in the South African (SA) population, stratified by public and private healthcare sectors. Methods. To measure the impact of COVID‐19, blood utilisation pre COVID‐19 was compared with an acute COVID‐19 period and a post‐acute period. Each period covered 450 days. Blood utilisation was evaluated against the background of reported COVID‐19 cases and national lockdown regulations. Results. With the onset of the COVID‐19 pandemic, overall RBC utilisation dropped by ~17%. The initial decline was more pronounced in the public sector. Overall, utilisation recovered to pre‐COVID‐19 levels in the post‐acute COVID‐19 period, but at different rates in the public and private sectors. Conclusion. There was a significant change in RBC utilisation during the course of the COVID‐19 pandemic, much more pronounced in the public sector, which services 85% of the SA population. During the post‐acute COVID‐19 period, blood utilisation recovered, but at a much slower rate in the public sector. The COVID‐19 pandemic accentuated the differences in blood utilisation patterns between the public and private healthcare sectors in SA, and each sector’s resilience in adapting to the challenges of the epidemic

(Re)connect social and environmental responsibility to learners’ living environments: Curriculum challenges and possible solutions for teaching-learning in Life Orientation

An analysis of the National Curriculum Statement indicates a strong focus on the promotion of knowledge in local context, whilst being sensitive to global imperatives. This implies that learning experiences must reflect local realities first; a call which compels teachers to adapt their teaching with the purpose to make learning relevant and meaningful for the learner. It is therefore an appropriate time to ask the key question: Do Life Orientation teachers (re)connect their teaching-learning on social and environmental responsibility with learners’ living environments to adhere to curriculum requirements of relevance and meaningfulness? The qualitative data obtained from 13 structured classroom observations, with specific reference to the topic social and environmental responsibility, revealed that Life Orientation teachers experienced challenges. Based on these qualitative findings the researchers propose place-based education as an appropriate teaching-learning strategy to (re)connect social and environmental responsibility with the learner’s living environment

FXYD2 and Na,K-ATPase Expression in Isolated Human Proximal Tubular Cells: Disturbed Upregulation on Renal Hypomagnesemia?

Autosomal dominant renal hypomagnesemia (OMIM 154020), associated with hypocalciuria, has been linked to a 121G to A mutation in the FXYD2 gene. To gain insight into the molecular mechanisms linking this mutation to the clinical phenotype, we studied isolated proximal tubular cells from urine of a patient and a healthy subject. Cells were immortalized and used to assess the effects of hypertonicity-induced overexpression of FXYD2 on amount, activity and apparent affinities for Na+, K+ and ATP of Na,K-ATPase. Both cell lines expressed mRNA for FXYD2a and FXYD2b, and patient cells contained both the wild-type and mutated codons. FXYD2 protein expression was lower in patient cells and could be increased in both cell lines upon culturing in hyperosmotic medium but to a lesser extent in patient cells. Similarly, hyperosmotic culturing increased Na,K-ATPase protein expression and ATP hydrolyzing activity but, again, to a lesser extent in patient cells. Apparent affinities of Na,K-ATPase for Na+, K+ and ATP did not differ between patient and control cells or after hyperosmotic induction. We conclude that human proximal tubular cells respond to a hyperosmotic challenge with an increase in FXYD2 and Na,K-ATPase protein expression, though to a smaller absolute extent in patient cells