Ultrafast laser pulse heating of metallic photocathodes and its contribution to intrinsic emittance

The heating of the electronic distribution of a copper photocathode due to an intense drive laser pulse is calculated under the two-temperature model using fluences and pulse lengths typical in RF photoinjector operation. Using the finite temperature-extended relations for the photocathode intrinsic emittance and quantum efficiency, the time-dependent emittance growth due to the same photoemission laser pulse is calculated. This laser heating is seen to limit the intrinsic emittance achievable for photoinjectors using short laser pulses and low quantum efficiency metal photocathodes. A pump-probe photocathode experiment in a standard 1.6 cell S-band gun is proposed, in which simulations show the time dependent thermal emittance modulation within the bunch from laser heating can persist for meters downstream and, in principle, be measured using a slice emittance diagnostic

Gamow-Jordan Vectors and Non-Reducible Density Operators from Higher Order S-Matrix Poles

In analogy to Gamow vectors that are obtained from first order resonance poles of the S-matrix, one can also define higher order Gamow vectors which are derived from higher order poles of the S-matrix. An S-matrix pole of r-th order at z_R=E_R-i\Gamma/2 leads to r generalized eigenvectors of order k= 0, 1, ... , r-1, which are also Jordan vectors of degree (k+1) with generalized eigenvalue (E_R-i\Gamma/2). The Gamow-Jordan vectors are elements of a generalized complex eigenvector expansion, whose form suggests the definition of a state operator (density matrix) for the microphysical decaying state of this higher order pole. This microphysical state is a mixture of non-reducible components. In spite of the fact that the k-th order Gamow-Jordan vectors has the polynomial time-dependence which one always associates with higher order poles, the microphysical state obeys a purely exponential decay law.Comment: 39 pages, 3 PostScript figures; sub2.eps may stall some printers and should then be printed out separately; ghostview is o.

Demonstration of single-shot picosecond time-resolved MeV electron imaging using a compact permanent magnet quadrupole based lens

We present the results of an experiment where a short focal length (~ 1.3 cm) permanent magnet electron lens is used to image micron-size features of a metal sample in a single shot, using an ultra- high brightness ps-long 4 MeV electron beam from a radiofrequency photoinjector. Magnifcation ratios in excess of 30x were obtained using a triplet of compact, small gap (3.5 mm), Halbach-style permanent magnet quadrupoles with nearly 600 T/m field gradients. These results pave the way to- wards single shot time-resolved electron microscopy and open new opportunities in the applications of high brightness electron beams.Comment: 5 pages, 6 figure

Determination of Fluorescence Polarization and Absorption Anisotropy in Molecular Complexes Having Threefold Rotational Symmetry

The current work concerns investigation of the polarization properties of complex molecular ensembles exhibiting threefold (C3) rotational symmetry, particularly with regard to the interplay between their structure and dynamics of internal energy transfer. We assume that the molecules or chromophores in such complexes possess strongly overlapped spectra both for absorption and fluorescence. Such trimeric structures are widely found in biological preparations, as for example the trimer of C-phycocyanin (C-PC). Higher order aggregates, e.g. hex-amers and three-hexamer rods, are also investigated and compared with the trimer case. The theory addresses both steady-state and 8-pulse excitation and establishes some links between them. Monochromophoric, bichro-mophoric and trichromophoric molecular complexes are individually examined. For steady-state excitation, analytical formulas are reported for the degree of fluorescence polarization and absorption anisotropy. It is shown that the polarization is dependent on the chromophore inclination relative to the symmetry axis, the relative efficiencies of absorption and fluorescence by chromophores of different spectral types, and the rates of energy equilibration. To assess the validity of the theory, it has been applied to C-PC aggregates. Here it was found that different C-PC aggregates provide practically identical polarization response. For S-pulse excitation we give analytical formulas for determination of the fluorescence depolarization, and also the depolarization associated with absorption recovery, both for a monochromophoric trimer and some particular cases of bichromophoric trimer. More complicated systems are analyzed by computer modeling. Thus it transpires that the initial polarization anisotropy r(t = 0) takes the value 0.4 for all considered aggregates; the long-time limit r(t →∞) has about the same value as is associated with steady-state excitation. We also show that with steady-state excitation the degree of fluorescence polarization is practically equal for various C3 aggregates of C-PC, and that the major factor determining the polarization is the chromophore orientation relative to the symmetry axis

The influence of teachers\u27 beliefs on literacy instruction for at-risk first graders: A multiple case study

Through a multiple case study design the influence of teachers\u27 beliefs on literacy instruction for at-risk first graders was examined and described. A volunteer sample included five female teachers who taught in different high risk schools within the same school district. Five research questions guided the study: (1) What are teachers\u27 beliefs about instructing young at-risk children to read and write? (2) What do teachers say they do as they instruct young at-risk children to read and write? (3) What do teachers actually do as they instruct young at-risk children to read and write? (4) What influences teachers\u27 instructional decisions as they teach young at-risk children to read and write? (5) Are there congruencies between teachers\u27 stated beliefs and their practice?;Data were collected from interviews, observations, questionnaires and a reflective activity over an academic year. Through the constant comparative method twelve general findings emerged: (1) Teachers must possess an understanding of the individual needs of at-risk children and address those needs; (2) Teachers must recognize and build on children\u27s individual strengths; (3) Teachers should nurture children\u27s enthusiasm for learning to read and write; (4) The learning process should begin at the appropriate developmental level; (5) At-risk children should be continuously stimulated in order to build confidence necessary for learning. A structured environment is important to accomplishing this goal; (6) At-risk children break the bonds of at-riskness by becoming literate; (7) Literacy instructional theory does not influence teachers\u27 practice as much as their beliefs; (8) There is no single method of literacy instruction for at-risk children, a combination of pedagogical approaches best serves their literacy needs; (9) Teacher modeling is a positive motivational factor for at-risk children learning to read and write; (10) All at-risk children can learn; Other findings indicated: (1) Teachers\u27 literacy instructional decisions are influenced by multiple factors; (2) There are congruencies between teachers\u27 stated beliefs and practice; The five case studies validate the work of previous researchers who suggested that teachers\u27 beliefs are an integral part of classroom practice. The teachers provided documented instances of the congruency between beliefs and classroom practice

Lean value marketing programs

Recent promotion of the National Pork Producers Lean Guide to Pork Value has focused attention on providing pork producers with an economic incentive to raise lean, meaty hogs. The packing industry version of this value-based idea is generally termed a grade and yield program. While each individual packing plant may have its own grade and yield program, several factors need to be considered when marketing hogs grade and yield, regardless of the packing plant. The preferred weight range for a packing plants grade and yield program must be known. Packing plants available to Kansas usually range from 210-240 lb or from 220-250 lb. If your hogs fall below or above the preferred weight range, price discounts will begin. Generally, the farther a pig is outside the preferred weight range, the greater the discount.; Swine Day, Manhattan, KS, November 20, 198

Cyclic dermal BMP signalling regulates stem cell activation during hair regeneration

In the age of stem cell engineering it is critical to understand how stem cell activity is regulated during regeneration. Hairs are mini-organs that undergo cyclic regeneration throughout adult life1, and are an important model for organ regeneration. Hair stem cells located in the follicle bulge2 are regulated by the surrounding microenvironment, or niche3. The activation of such stem cells is cyclic, involving periodic -catenin activity4, 5, 6, 7. In the adult mouse, regeneration occurs in waves in a follicle population, implying coordination among adjacent follicles and the extrafollicular environment. Here we show that unexpected periodic expression of bone morphogenetic protein 2 (Bmp2) and Bmp4 in the dermis regulates this process. This BMP cycle is out of phase with the WNT/-catenin cycle, thus dividing the conventional telogen into new functional phases: one refractory and the other competent for hair regeneration, characterized by high and low BMP signalling, respectively. Overexpression of noggin, a BMP antagonist, in mouse skin resulted in a markedly shortened refractory phase and faster propagation of the regenerative wave. Transplantation of skin from this mutant onto a wild-type host showed that follicles in donor and host can affect their cycling behaviours mutually, with the outcome depending on the equilibrium of BMP activity in the dermis. Administration of BMP4 protein caused the competent region to become refractory. These results show that BMPs may be the long-sought 'chalone' inhibitors of hair growth postulated by classical experiments. Taken together, results presented in this study provide an example of hierarchical regulation of local organ stem cell homeostasis by the inter-organ macroenvironment. The expression of Bmp2 in subcutaneous adipocytes indicates physiological integration between these two thermo-regulatory organs. Our findings have practical importance for studies using mouse skin as a model for carcinogenesis, intra-cutaneous drug delivery and stem cell engineering studies, because they highlight the acute need to differentiate supportive versus inhibitory regions in the host skin

The Legionella effector WipB is a translocated Ser/Thr phosphatase that targets the host lysosomal nutrient sensing machinery

Legionella pneumophila infects human alveolar macrophages and is responsible for Legionnaire’s disease, a severe form of pneumonia. L. pneumophila encodes more than 300 putative effectors, which are translocated into the host cell via the Dot/Icm type IV secretion system. These effectors highjack the host’s cellular processes to allow bacterial intracellular growth and replication. Here we adopted a multidisciplinary approach to investigate WipB, a Dot/Icm effector of unknown function. The crystal structure of the N-terminal domain at 1.7 Å resolution comprising residues 25 to 344 revealed that WipB harbours a Ser/Thr phosphatase domain related to the eukaryotic phospho-protein phosphatase (PPP) family. The C-terminal domain (residues 365–524) is sufficient to pilot the effector to acidified LAMP1-positive lysosomal compartments, where WipB interacts with the v-ATPase and the associated LAMTOR1 phosphoprotein, key components of the lysosomal nutrient sensing (LYNUS) apparatus that controls the mammalian target of rapamycin (mTORC1) kinase complex at the lysosomal surface. We propose that WipB is a lysosome-targeted phosphatase that modulates cellular nutrient sensing and the control of energy metabolism during Legionella infection